维普中文期刊产品整合服务

Single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling

查看全文 作　　者：Min [1,2,3]Wei;Yanping [1,2,3]Sun;Shouzhen [1,2,3]Li;Yunuo [1,2,3]Chen;Longfei [1,2,3]Li;Minghao [1,2,3]Fang;Ronghua [3]Shi;Dali [2,3]Tong;Jutao [1,2,3]Chen;Yuqian [1,2,3]Ma;Kun [1,2,3]Qu;Mei [1,2,3]Zhang;Tian [1,2,3,4,5]Xue 高影响力作者 机构地区：[1]Division of Life Sciences and Medicine,Department of Ophthalmology,The First Affiliated Hospital of USTC,University of Science and Technology of China,Hefei 230026,China;[2]Hefei National Research Center for Physical Sciences at the Microscale,Neurodegenerative Disorder Research Center,CAS Key Laboratory of Brain Function and Disease,University of Science and Technology of China,Hefei 230026,China;[3]Division of Life Sciences and Medicine,School of Life Sciences,University of Science and Technology of China,Hefei 230026,China;[4]Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences,Shanghai 200031,China;[5]Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100864,China高影响力机构 出　　处：《Protein & Cell》索引2023年第14卷第8期,共15页高影响力期刊 摘　　要：Light adaptation enables the vertebrate visual system to operate over a wide range of ambient illumination.Regulation of phototransduction in photoreceptors is considered a major mechanism underlying light adaptation.However,various types of neurons and glial cells exist in the retina,and whether and how all retinal cells interact to adapt to light/dark conditions at the cellular and molecular levels requires systematic investigation.Therefore,we utilized single-cell RNA sequencing to dissect retinal cell-type-specific transcriptomes during light/dark adaptation in mice.The results demonstrated that,in addition to photoreceptors,other retinal cell types also showed dynamic molecular changes and specifically enriched signaling pathways under light/dark adaptation.Importantly,Müller glial cells(MGs)were identified as hub cells for intercellular interactions,displaying complex cell‒cell communication with other retinal cells.Furthermore,light increased the transcription of the deiodinase Dio2 in MGs,which converted thyroxine(T4)to active triiodothyronine(T3).Subsequently,light increased T3 levels and regulated mitochondrial respiration in retinal cells in response to light conditions.As cones specifically express the thyroid hormone receptor Thrb,they responded to the increase in T3 by adjusting light responsiveness.Loss of the expression of Dio2 specifically in MGs decreased the light responsive ability of cones.These results suggest that retinal cells display global transcriptional changes under light/dark adaptation and that MGs coordinate intercellular communication during light/dark adaptation via thyroid hormone signaling. 关 键 词：single cell Müller glial cells intercellular communication light/dark adaptation thyroid hormone signaling
相关文献

参考文献(61)

引证文献(2)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费