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| 1 | PARylation regulates stress granule dynamics, phase separation, and neurotoxicity of disease-related RNA-binding proteins显示文摘Mutations in RNA-binding proteins (RBPs) localized in ribonucleoprotein (RNP) granules, such as hnRNP A1 and TDP-43, promote aberrant protein aggregation, which is a pathological hallmark of various neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Protein posttranslational modifications (PTMs) are known to 佗gulate RNP granules. In this study, we investigate the function of poly(ADP-ribosyl)ation (PARylation), an important PTM involved in DNA damage repair and cell death, in RNP granule-related neurodegeneration. We reveal that PARylation levels are a major regulator of the assembly-disassembly dynamics of RNP granules containing disease-related RBPs, hnRNP A1 and TDP-43. We find that hnRNP A1 can both be PARylated and bind to PARylated proteins or poly(ADP-ribose)(PAR). We further uncover that PARylation of hnRNP A1 at K298 controls its nucleocytoplasmic transport, whereas PAR-binding via the PAR-binding motif (PBM) of hnRNP Al regulates its association with stress granules. Moreover, we reveal that PAR not only dramatically enhances the liquid-liquid phase separation of hnRNP A1, but also promotes the co-phase separation of hnRNP A1 and TDP-43 in vitro and their interaction in vivo. Finally, both genetic and pharmacological inhibition of PARP mitigates hnRNP Al- and TDP-43-mediated neurotoxicity in cell and Drosophila models of ALS. Together, our findings suggest a novel and crucial role for PARylation in regulating the dynamics of RNP granules, and that dysregulation in PARylation and PAR levels may con tribute to ALS disease pathoge nesis by promoting protei n aggregation. | Yongjia Duan Aiying Du Jinge Gu Gang Duan Chen Wang Xinrui Gui Zhiwei Ma Beituo Qian Xue Deng Kai Zhang Le Sun Kuili Tian Yaoyang Zhang Hong Jiang Cong Liu Yanshan Fang | 2019 | Cell Research2019，29，3： | 13 |
| 2 | Comprehensive analysis of NAC transcription factors in diploid Gossypium: sequence conservation and expression analysis uncover their roles during fiber development显示文摘Determining how function evolves following gene duplication is necessary for understanding gene expansion.Transcription factors(TFs)are a class of proteins that regulate gene expression by binding to specific cis-acting elements in the promoters of target genes,subsequently activating or repressing their transcription.In the present study,we systematically examined the functional diversification of the NAC transcription factor(NAC-TFs)family by analyzing their chromosomal location,structure,phylogeny,and expression pattern in Gossypium raimondii(Gr)and G.arboreum(Ga).The 145 and 141 NAC genes identified in the Gr and Ga genomes,respectively,were annotated and divided into 18 subfamilies,which showed distinct divergence in gene structure and expression patterns during fiber development.In addition,when the functional parameters were examined,clear divergence was observed within tandem clusters,which suggested that subfunctionalization had occurred among duplicate genes.The expression patterns of homologous gene pairs also changed,suggestive of the diversification of gene function during the evolution of diploid cotton.These findings provide insights into the mechanisms underlying the functional differentiation of duplicated NAC-TFs genes in two diploid cotton species. | Haihong Shang Zhongna Wang Changsong Zou Zhen Zhang Weijie Li Junwen Li Yuzhen Shi Wankui Gong Tingting Chen Aiying Liu Juwu Gong Qun Ge Youlu Yuan | 2016 | Science China(Life Sciences)2016，59，2： | 12 |
| 3 | Influence of Substrate Negative Bias on Structure and Properties of TiN Coatings Prepared by Hybrid HIPIMS Method显示文摘TiN coatings were deposited using a hybrid home-made high power impulse magnetron sputtering(HIPIMS)technique at room temperature.The effects of substrate negative bias voltage on the deposition rate,composition,crystal structure,surface morphology,microstructure and mechanical properties were investigated.The results revealed that with the increase in bias voltage from-50 to-400 V,TiN coatings exhibited a trend of densification and the crystal structure gradually evolved from(111) orientation to(200)orientation.The growth rate decreased from about 12.2 nm to 7.8 nm per minute with the coating densification.When the bias voltage was-300 V,the minimum surface roughness value of 10.1 nm was obtained,and the hardness and Young's modulus of TiN coatings reached the maximum value of 17.4 GPa and 263.8 GPa,respectively.Meanwhile,the highest adhesion of 59 N was obtained between coating and substrate. | Zhenyu Wang Dong Zhang Peiling Ke Xincai Liu Aiying Wang | 2015 | Journal of Materials Science & Technology2015，31，1： | 10 |
| 4 | Preparation of Ti_2AlC MAX Phase Coating by DC Magnetron Sputtering Deposition and Vacuum Heat Treatment显示文摘Due to the excellent corrosion resistance and high irradiation damage resistance,Ti_2AlC MAX phase is considered as a candidate for applications as corrosion resistant and irradiation resistant protective coating.MAX phase coatings can be fabricated through firstly depositing a coating containing the three elements M,A,and X close to stoichiometry of the MAX phases using physical vapor deposition,followed by heat treatment in vacuum.In this work,Ti-Al-C coating was prepared on austenitic stainless steels by reactive DC magnetron sputtering with a compound Ti_(50)Al_(50) target,and CH4 used as the reactive gas.It was found that the as-deposited coating is mainly composed of Ti_3AlC antiperovskite phase with supersaturated solid solution of Al.Additionally,the ratio of Ti/Al remained the same as that of the target composition.Nevertheless,a thicker thermally grown Ti_2AlC MAX phase coating was obtained after being annealed at 800℃ in vacuum for 1 h.Meanwhile,the ratio of Ti/Al became close to stoichiometry of Ti_2AlC MAX phases.It can be understood that owing to the higher activity of Al,it diffused quickly into the substrate during annealing,and then more stable Ti_2AlC MAX phases transformed from the Ti_3AlC antiperovskite phase. | Zongjian Feng Peiling Ke Aiying Wang | 2015 | Journal of Materials Science & Technology2015，31，12： | 10 |
| 5 | Correlation between BRAF^(V600E) mutation and clinicopathological features in pediatric papillary thyroid carcinoma显示文摘In adults, the presence of the BRAF^(V600E) mutation in papillary thyroid cancer(PTC) has been demonstrated to be strongly associated with aggressive cancer-cell characteristics and poor patient prognosis. In contrast, the frequency of this mutation in pediatric PTC has undergone limited study, and the few available estimates range from 0 to 63%. Furthermore, the role of the BRAF^(V600E) mutation in pediatric PTC is controversial; thus, the present study aimed to investigate the prevalence and role of the BRAF^(V600E) mutation in48 pediatric patients with PTC, aged 3–13 years. Of these patients, 41 were diagnosed with classic PTC, five were found to have a follicular variant of PTC, and two to exhibit a diffuse sclerosing PTC variant. The BRAF^(V600E) mutation was identified to be present in 35.4% of the 48 analyzed patients, and in 41.5% of the patients diagnosed with classical PTC. Furthermore, the presence of the BRAF^(V600E) mutation was found to be associated with a patient age at diagnosis of less than ten years(P=0.011), the performance of a thyroidectomy(P=0.03), exhibited tumor multifocality(P=0.02) and/or extra-thyroidal invasion(P=0.003), and both a low MACIS(Metastases, Age, Completeness of resection, Invasion, Size)(P=0.036) and AMES(Age, Metastasis, Extent of tumor,Size)(P=0.001)score. Together, these data suggest that the presence of the BRAF^(V600E) mutation may be negatively correlated with partial aggressive clinicopathological features of pediatric PTC. | Jiangqiao Geng Huanmin Wang Yuanhu Liu Jun Tai Yaqiong Jin Jie Zhang Lejian He Libing Fu Hong Qin Yingluan Song Jinzhu Su Aiying Zhang Xin Wen Yongli Guo Xin Ni | 2017 | Science China(Life Sciences)2017，60，7： | 9 |
| 6 | Manipulation of SlMXl for enhanced carotenoids accumulation and drought resistance in tomato显示文摘Plants are the ultimate source of nutrients in the human diet. To ensure adequate availability of high quality food for an increasing world population, traits including improved tolerance of stresses and nutrient levels need to be selected in crops, both individually and in combination. Here we report the identification of Sl MX1 encoding a MIXTA-like MYB transcription factor in tomato that simultaneously modulates drought resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways. Over-expression of Sl MX1 results in substantially increased drought tolerance and improved fruit quality, while knocking down Sl MX1 resulted in the opposite phenotypes. Our study indicates an effective way with multiple beneficial traits by genetic engineering of a single regulatory gene and can be a novel approach to breeding crops. | Mohamed Ewas Yanqiang Gao Shouchuang Wang Xianqing Liu Hongyan Zhang Elsayed M. E. Nishawy Farhan Aii Raheel Shahzad Khurram Ziaf Hizar Subthain Cathie Martin Jie Luo | 2016 | Science Bulletin2016，61，18： | 9 |
| 7 | Erosion behavior and failure mechanism of Ti/TiAlN multilayer coatings eroded by silica sand and glass beads显示文摘Severe erosion by hard particles is a crucial problem to engine blades when aircraft take off and land in harsh environments, especially for the developed lightweight titanium alloy components. Here, we deposited the Ti/TiAlN multilayer coatings with various cycles on Ti–6 Al–4 V substrates by a home-made hybrid multisource cathodic arc system. The effects of the silica sand and glass beads on erosion behavior of the coatings were focused. Results showed that the Ti/TiAlN multilayer coatings eroded by the silica sand exhibited the predominant 'layer by layer' failure mechanism. In particular, increasing the number of cycles led to the dramatic increase in erosion rate for Ti/TiAlN multilayer coatings, due to the deterioration of their mechanical properties. Different from the silica sand case, however, the erosion rate of the coatings treated by glass beads indicated faint dependence upon the number of cycles, where the coating failure was dominated by the 'piece by piece' failure mechanism. Noted that the Ti layers along with the formed interfaces enhanced the erosion resistance of the coatings, although the failure mechanisms were differently eroded by silica sand and glass beads. Meanwhile, the Ti layers and interfaces hindered the propagation of radial cracks and restrained the lateral cracks within one single TiAlN layer. | Jintao Shuai Xiao Zuo Zhenyu Wang Lili Sun Rende Chen Li Wang Aiying Wang Peiling Ke | 2021 | Journal of Materials Science & Technology2021，，21： | 8 |
| 8 | Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage显示文摘To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat(SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum×Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centi Morgan(c M) of the cotton AD genome, with an average marker interval of 2.23 c M. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci(QTLs) for lint percentage(LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%–2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality. | Yuzhen Shi Wentan Li Aiguo Li Ruihua Ge Baocai Zhang Junzhi Li Guangping Liu Junwen Li Aiying Liu Haihong Shang Juwu Gong Wankui Gong Zemao Yang Feiyü Tang Zhi Liu Weiping Zhu Jianxiong Jiang Xiaonan Yu Tao Wang Wei Wang Tingting Chen Kunbo Wang Zhengsheng Zhang Youlu Yuan | 2015 | Journal of Integrative Plant Biology2015，57，5： | 7 |
| 9 | Structural design of Cr/GLC films for high tribological performance in artificial seawater:Cr/GLC ratio and multilayer structure显示文摘In this paper,graphite-like carbon(GLC)films with Cr buffer layer were fabricated by DC magnetron sputtering technique with the thickness ratio of Cr to GLC films varying from 1:2 to 1:20.The effect of Cr/GLC modulation ratio on microstructure,mechanical and tribological properties in artificial seawater was mainly investigated by scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),nano-indenter and a reciprocating sliding tribo-meter.The propagation of defects plays an important role in the evolution of delamination,which is critical to wear failure of GLC films in artificial seawater.Designing the proper multilayer structure could inhibit the defects propagation and thus protect the basis material.The multilayer Cr/GLC film with optimized ratio of 1:3 demonstrates a low average friction coefficient of 0.08±0.006 and wear rate of(2.3±0.3)×10^(-8)mm^3/(N m)in artificial seawater,respectively. | Lei Li Peng Guo Lin-Lin Liu Xiaowei Li Peiling Ke Aiying Wang | 2018 | Journal of Materials Science & Technology2018，34，8： | 7 |
| 10 | Simulations of the Dependence of Gas Physical Parameters on Deposition Variables during HFCVD Diamond Films显示文摘在钻石拍摄的热细丝化学药品蒸汽免职(HFCVD ) 的生长期间,在 2-D 的数字模拟数学模型被雇用在煤气的物理参数上调查各种各样的免职参数的影响包括温度,气体的速度和卷密度。甚至在优化免职参数的情况中,煤气的参数的空间分布是异构的,这被发现首先对热阻塞到期来自热细丝和低温实验法的泵效果从冷反应堆墙产生。卷密度的分发与热圆流动的现象同意了很好,到获得高生长的关键障碍之一在 HFCVD 评价进程。在有高温度或反应堆墙的断热的边界状况的等温的边界的优点,然而, thermalround 流动深刻地被减少并且作为后果,煤气的物理参数的一致性更加被改进,由试验性的电影生长识别了。 | Aiying WANG Kwangryeol Lee Chao SUN Lishi WEN | 2006 | Journal of Materials Science & Technology2006，22，5： | 3 |
| 11 | Synthesis and Fungicidal Activity of (E)-5-[1-(2-Oxo- 1 -oxaspiro[4,5]dec/non-3-en-3-yl)ethylidene]-2- aminoimidazolin-4-one Derivatives显示文摘 | Bo Tang Aiying Guan Yu Zhao Jiazhen Jiang Mingan Wang Ligang Zhou | 2017 | Chinese Journal of Chemistry2017，35，7： | 3 |
| 12 | Microstructure, mechanical and tribological behaviors of MoS 2 -Ti composite coatings deposited by a hybrid HIPIMS method显示文摘 | Xiaopeng Qin Peiling Ke Aiying Wang Kwang Ho Kim | 2013 | Surface & Coatings Technology2013，，： | 2 |
| 13 | Effect of Negatively Charged Ions on the Formation of Microarc Oxidation Coating on 2024 Aluminium Alloy显示文摘The present study deals with the effect of negatively charged ions on the ceramic coating formation on 2024 aluminium alloy during microarc oxidation (MAO) process. On the basis of the experimental results, two steps (the formation of an incipient film without arc presence and the growth of a ceramic coating with arc discharge) of MAO process have been observed. For comparison, four different negatively charged ions studied. It is proved that negatively charged ions strongly participated in the formation of an incipient film with high impedance value at the first step. The growth of ceramic coating depends on the combination between Al of the substrate and O from the electrolyte, and the negatively charged ions are little consumed. As an anodic oxide coating is prepared on the sample surface instead of the incipient film, the first step occurs easily and the growth of ceramic coating is accelerated. Furthermore, the mechanism of negatively charged ions in the formation of the MAO coating has been proposed. | Wei Yang Bailing Jiang Aiying Wang Huiying Shi | 2012 | Journal of Materials Science & Technology2012，28，8： | 2 |
| 14 | Phase orientation improved the corrosion resistance and conductivity of Cr_(2)AlC coatings for metal bipolar plates显示文摘In view of the M_(n+1)AX_(n)(MAX)phase coatings benefting the adaptive passivation flm for good corrosion resistance and high electronic density of states for excellent electrical conductivity,here,we reported the Cr_(2)Al C MAX phase coatings with different preferred orientations by a homemade technique consisting of vacuum arc and magnetron sputtering.The dependence of surface and interface microstructural evolution upon the corrosion and electrochemical properties of deposited coating was focused.Results showed that all the Cr_(2)Al C coatings with different phase orientations greatly improved the performance of stainless steel(SS)316 L substrate.Specifcally,the lowest value of interface contact resistance(ICR)reached to 3.16 mΩcm^(2)and the lowest corrosion current density was 2×10^(-2)μA cm^(-2),which were much better than those of bare SS316L.The combined studies of electrochemical properties and theoretical calculations demonstrated that the Cr_(2)Al C coatings with preferred(103)orientation were easier to form oxide passivation flm on their surface to increase the corrosion resistance. | Guanshui Ma Dong Zhang Peng Guo Hao Li Yang Xin Zhenyu Wang Aiying Wang | 2022 | Journal of Materials Science & Technology2022，，10： | 2 |
| 15 | Microstructure and Tribological Behavior of Self-lubricating(Si:N)-DLC/MAO Coatings on AZ80 Magnesium Substrate显示文摘The combined micro arc oxidation(MAO)and a hybrid beam deposition process was used to deposit duplex(Si:N)-DLC/MAO coatings on AZ80 magnesium alloy.The microstructure and composition of the duplex coatings were analyzed by Raman spectroscopy,X-ray photoelectron spectroscope(XPS),scanning electron microscope(SEM)and atomic force microscopy(AFM).Tribological behaviors of the coatings were studied by ball-on-disk friction test.It was found that the ID/IG ratio of the(Si:N)-DLC(diamond-like carbon)top films increases with decreasing C2H2/N2ratio.The(Si:N)-DLC top film with Si3N4was formed on the MAO coated sample as the C2H2/N2ratio was 10sccm:5sccm,which showed an increasing critical load compared with the pure DLC directly deposited on the Mg alloy substrate.As a result,the(Si:N)-DLC/MAO coating exhibited an advanced wear protection for the substrate. | Wei YANG Zhennan DENG Dong ZHANG Peiling KE Aiying WANG | 2013 | Acta Metallurgica Sinica(English Letters)2013，26，6： | 2 |
| 16 | Insights on high temperature friction mechanism of multilayer ta-C films显示文摘In this work,the high temperature friction mechanism of the tetrahedral amorphous carbon(ta-C)film was elucidated.The multilayer ta-C film with alternating hard and soft sub-layers exhibited a low friction coefficient of 0.14 at 400℃ before a sudden failure occurred at 4600 cycles.The wear failure was attributed to the gradual consumption of the ta-C film at the contact region.The design of a hard or soft top layer effectively regulated the high temperature friction properties of the multilayer ta-C.The addition of a hard top layer contributed to a low friction coefficient(0.11)and a minor wear rate(4.0×10^(-7)mm^(3)/(N m)),while a soft top layer deteriorated the lubrication effect.It was proposed that the passivation of dangling bonds at the sliding interface dominated the low-friction mechanism of the ta-C film at high temperature,while the friction induced graphitization and the formation of sp^(2)-rich carbonaceous transfer layer triggered C-C inter-film bonding,resulting in serious adhesion force and lubrication failure.Moreover,the multilayer ta-C film with hard top layer obtained excellent friction performance within 500℃,while the high temperature induced oxidation and volatilization of carbon atoms led to the wear failure at 600℃. | Jing Wei Peng Guo Hao Li Peiling Ke Aiying Wang | 2022 | Journal of Materials Science & Technology2022，，2： | 2 |
| 17 | Melt Flow Properties,Thermal Properties and Morphology of Polycarbonate/Highly Branched Polystyrene Blends显示文摘 | Aiying Li Jieyun Chang Kaiquan Wang Lude Lu Xujie Yang Xin Wang | 2006 | Polymer international2006，55，5： | 1 |
| 18 | Metformin inhibits ceil proliferation, migration and invasion by attenuating CSC function mediated by deregulating miRNAs in pancreatic cancer cel]s显示文摘 | Bao B Wang Z AIi S | 2012 | Cancer Prey Res (Phila)2012，5，3： | 1 |
| 19 | Recent developments of the high strength and high ductility nanostructured materials显示文摘This talk will summarize the recent work related to a kind of new nanomaterials produced by the SMAT (surface mechanical attrition treatment).The concept of surface nanocrystallization of materials will be presented.In terms of the grain refinement mechanism induced by plastic deformation,a novel surface mechanical attrition(SMA) technique was developed for synthesizing a nanostructured surface layer on metallic materials in order to upgrade the overall properties and performance.The grain refinement mechanism of the surface layer during the SMA treatment will be analyzed in terms of the nanostructure observations in several typical materials.Very high yield stress(5 times of the base material) on the surface layer of the material obtained by the SMAT has been observed.The effect of surface nanostructures on the mechanical behavior and on the failure mechanism of metallic material shows the possibility to develop a new strength gradient composite using co-rolling and nitriding.The role of residual stress induced during the treatment will be investigated and discussed.The developed materials are also porosity free materials which can be used as reference material for the local mechanical behavior investigation technique such as the nanoindentation.A general concept for obtaining high strength and high ductility nanostructured materials will be presented.The exceptional high strength and high ductility steels have developed.The simulation of the mechanisms for improving ductility of high strength nanostructured materials will be presented.The potential applications for the land transportation vehicles(car,bus,train) and wind energy have been investigated.Some examples of concept design for the integration of the advanced nanostructured steels will be presented. | Jian LU,Aiying CHEN,Hongning KOU,Ying LI,Leyu WANG and Chunsheng WEN Department of Mechanical Engineering,The Hong Kong Polytechnic University,Hung Hom Kowloon,Hong Kong,China | 2010 | Baosteel Technical Research2010，4，S1： | 1 |
| 20 | Investigation of the microstructure, mechanical properties and tribological behaviors of Ti-containing diamond-like carbon films fabricated by a hybrid ion beam method显示文摘 | Wei Dai Peiling Ke Myoung-Woon Moon Kwang-Ryeol Lee Aiying Wang | 2012 | Thin Solid Films2012，，19： | 1 |
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