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| 1 | 苎麻遗传转化再生体系的建立显示文摘苎麻(BoehmerianiveaL.Guad)是荨麻科苎麻属宿根型草本植物,主要分布在热带和亚热带地区,为我国特产,是重要的纺织纤维和饲料作物。由于苎麻为杂合体,其遗传背景十分复杂,有性杂交等常规方法难以取得育种上的突破,因此,探讨利用基因工程技术改良苎麻品种具有重要意义。本研究以苎麻品种圆叶青叶盘为外植体,选用MS培养基为基本培养基,附加激素BA、IAA、GA3和NAA,通过研究不同激素浓度组合对外植体愈伤诱导及不定芽分化的影响以及不同激素配比对生根的作用,建立了较好的遗传转化再生体系。试验结果表明圆叶青叶盘最适诱导愈伤培养基为MS+6-BA2.0mg/L+GA30.4mg/L+IAA0.2mg/L+蔗糖30g/L+琼脂7.5g/L;分化培养基为MS+6-BA2.0mg/L+GA30.4mg/L+蔗糖30g/L+琼脂7.5g/L;小苗的最佳生根培养基为MS+NAA0.2mg/L+IAA0.1mg/L+蔗糖30g/L+琼脂7.5g/L。在抗生素浓度梯度筛选实验中获得50mg/L卡那霉素可以抑制叶盘的分化;40mg/L卡那霉素可以抑制再生植株的生根。筛选出的遗传转化再生体系,为进一步利用基因工程技术对苎麻进行遗传改良打下基础。 | 孔华 郭安平 章霄云 姚庆荣 郭运玲 贺立卡 Kong Hua Guo Anping Zhang Xiaoyun Yao Qingrong Guo Yunling He Lika | 2006 | 分子植物育种2006，4，2： | 16 |
| 2 | OsSPL18 controls grain weight and grain number in rice显示文摘Grain weight and grain number are two important traits directly determining grain yield in rice. To date,a lot of genes related to grain weight and grain number have been identified; however, the regulatory mechanism underlying these genes remains largely unknown. In this study, we studied the biological function of OsSPL18 during grain and panicle development in rice. Knockout (KO) mutants of OsSPL18exhibited reduced grain width and thickness, panicle length and grain number, but increased tiller number. Cytological analysis showed that OsSPL18 regulates the development of spikelet hulls by affecting cell proliferation. qRT-PCR and GUS staining analyses showed that OsSPL18 was highly expressed in developing young panicles and young spikelet hulls, in agreement with its function in regulating grain and panicle development. Transcriptional activation experiments indicated that OsSPL18is a functional transcription factor with activation domains in both the N-terminus and C-terminus, and both activation domains are indispensable for its biological functions. Quantitative expression analysis showed that DEP1, a major grain number regulator, was significantly down-regulated in OsSPL18 KO lines.Both yeast one-hybrid and dual-luciferase (LUC) assays showed that OsSPL18 could bind to the DEP1promoter, suggesting that OsSPL18 regulates panicle development by positively regulating the expression of DEP1. Sequence analysis showed that OsSPL18 contains the OsmiR156k complementary sequence in the third exon; 5?RLM-RACE experiments indicated that OsSPL18 could be cleaved by OsmiR156k. Taken together, our results uncovered a new OsmiR156k-OsSPL18-DEP1 pathway regulating grain number in rice. | Hua Yuan Peng Qin Li Hu Shijie Zhan Shifu Wang Peng Gao Jing Li Mengya Jin Zhengyan Xu Qiang Gao Anping Du Bin Tu Weilan Chen Bingtian Ma Yuping Wang Shigui Li | 2019 | Journal of Genetics and Genomics2019，46，1： | 8 |
| 3 | Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2显示文摘Euglena gracilis is a unicellular green eukaryotic microalga that features characteristics of both plants and animals.The photosynthetic function of its chloroplast is easily lost under stress resulting in bleached mutants,while the physiological role of their residual plastid DNAs remains unclear.In this study,we obtained five bleached mutants by ofloxacin(Ofl)treatment,identified 12 residual plastid genes in five bleached mutants,and determined the mRNA levels in the wild type E.gracilis(WT)and one bleached mutant(OflB2)under dark and light stimulation conditions by quantitative reverse transcribed PCR(qRTPCR).Results show that the expression of all selected plastid genes in both WT and OflB2 mutant did not change significantly in darkness,while their responses to light stimulation were different.Under the light stimulation conditions,half of the genes did not change significantly,while most of the other genes were down-regulated in OflB2 mutant and up-regulated in WT.Therefore,the bleached mutant retains part of the plastid genome and the plastid relic is responsive to light.Our research will help to understand the functions of residual plastid DNA and evolution of chloroplasts. | QIN Huan GUO Qingqing LIU Chenchen LI Fenglan ZHANG Hua CHU Zihan WANG Jiangxin LEI Anping | 2020 | Journal of Oceanology and Limnology2020，38，6： | 0 |
| 4 | Twelve-week study of moxa smoke: occupational exposure in female rats显示文摘OBJECTIVE: To assess the toxicity of moxa smoke in rats.METHODS: Forty-eight female Wister rats were randomly divided into 4 groups(n = 12/group) to simulate moxa smoke exposure in Chinese medicine clinics(CMCs): the control group, and three moxa-smoke exposed groups of PM10 mass concentrations 3-5, 7-9 and 27-30 mg/m^3, respectively.These concentrations were 1 ×, 2-3 ×, and 7-9 ×fold the concentrations found in CMCs. Exposures continued for 12 weeks(200 min/d, 5 d/week).RESULTS: No deaths were noted. After the exposure, the body weights, ratios of organ weight to body weight, urinary parameters, hematological parameters, clinical chemistry parameters and microscopic examinations revealed no obvious toxicity.CONCLUSION: Moxa smoke did not induce toxic effects in female rats in the study. These findings provide new evidence to the toxicity of moxa smoke. | Han Li Huang Chang Yang Jia Wang Lei Bai Hua Liu Juntian Jian Huang Liu Ping Lim MinYee Ju Dahong Lao Lixing Liu Changxi Zhao Baixiao Sun Wen Xu Anping Yang Xiuyan Zhang Cong He Rui | 2019 | Journal of Traditional Chinese Medicine2019，39，2： | 0 |
| 5 | Fracture behavior dependent on crack-tip shapes in nanoscale crack-defect monolayer boron nitride sheets显示文摘Nanoscale defects,including cracks,circular holes,and the triangular-shaped defects,often occur in the growth of boron nitride nanosheets(BNNS).In this study,the fracture behavior of chiral BNNS with different crack-tip shapes and the interactions of nanoscale crack-defects are studied using molecular dynamics(MD)simulations and finite element(FE)analysis.Both MD and FE results indicate that the fracture strength of BNNS with two crack tips(t=2)is significantly higher than that with one crack tip(t=1),in which the difference in zigzag(ZZ)direction is more obvious than that in armchair(AC)direction,mainly due to the fact that the change of bond angles near the crack tips is more substantial in the ZZ direction than those in the AC direction.Our results show that the fracture strength of BNNS strongly depends on crack-tip shapes,chiral angles,the defect-to-crack tip spacing and deflection angles.Checking against the current MD simulations and FE analysis shows the present results are reasonable.This study should be of great importance for enhancing the fracture performance of BNNS by modulating their crack-tip shapes and the interactions of nanoscale crack-defects. | Wentao Su Chunhua Zhu Anping Hua Shanchen Li Junhua Zhao | 2021 | International Journal of Smart and Nano Materials2021，12，1： | 0 |
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