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| 1 | Subtyping Animal Influenza Virus with General Multiplex RT-PCR and Liquichip High Throughput (GMPLex)显示文摘This study developed a multiplex RT-PCR integrated with luminex technology to rapidly subtype simultaneously multiple influenza viruses. Primers and probes were designed to amplify NS and M genes of influenza A viruses HA gene of H1, H3, H5, H7, H9 subtypes, and NA gene of the N1 and N2 subtypes. Universal super primers were introduced to establish a multiplex RT-PCR (GM RT-PCR). It included three stages of RT-PCR amplification, and then the RT-PCR products were further tested by LiquiChip probe, combined to give an influenza virus (IV) rapid high throughput subtyping test, designated as GMPLex. The IV GMPLex rapid high throughput subtyping test presents the following features: high throughput, able to determine the subtypes of 9 target genes in H1, H3, H5, H7, H9, N1, and N2 subtypes of the influenza A virus at one time; rapid, completing the influenza subtyping within 6 hours; high specificity, ensured the specificity of the different subtypes by using two nested degenerate primers and one probe, no cross reaction occurring between the subtypes, no non-specific reactions with other pathogens and high sensitivity. When used separately to detect the product of single GM RT-PCR for single H5 or N1 gene, the GMPLex test showed a sensitivity of 10-5(= 280ELD50) forboth tests and the Luminex qualitative ratio results were 3.08 and 3.12, respectively. When used to detect the product of GM RT-PCR for H5N1 strain at the same time, both showed a sensitivity of 10-4(=2800 ELD50). The GMPLex rapid high throughput subtyping test can satisfy the needs of influenza rapid testing. | Zhi-feng Qin Jie Sun Ti-kang Lu Shao-ling Zeng Qun-yi Hua Qing-yan Ling Shu-kun Chen Jian-qiang Lv Cai-hong Zhang Bing Cheng Zhou-xi Ruan Ying-zuo Bi Joseph J Giambrone Hong-zhuan Wu | 2012 | Virologica Sinica2012，27，2： | 8 |
| 2 | Soil phosphorus bioavailability assessed by XANES and Hedley sequential fractionation technique in a glacier foreland chronosequence in Gongga Mountain, Southwestern China显示文摘The primary productivity of terrestrial ecosystems is influenced by soil phosphorus bioavailability, which depends largely on chemical fractions of phosphorus. The sequential fractionation technique developed by Hedley et al. or its subsequent modification is a well-known method to determine soil phosphorus forms. Hedley sequential fractionation technique separates the phosphorus into fractions based on their different chemical solubilities in extractants with certain chemical properties. Recently, synchrotron-based X-ray absorption near edge structure(XANES) spectroscopy has been employed to measure soil phosphorus species directly and non-invasively. The XANES method provides information concerning local structure and chemical information of target elements at a molecular level. Thus, it can distinguish phosphorus fractions bound by metal oxides or hydroxides(such as Fe, Al, and Ca). In this present work, the phosphorus speciation of topsoil along a glacial foreland chronosequence in Gongga Mountain is determined using these two methods. The changes in soil phosphorus bioavailability along the 120-year-old chronosequence are assessed based on comparisons of the results obtained by these two methods. The results indicate that Hedley sequential fractionation technique shows a greater ability to determine soil bioavailable phosphorus(Resin-P and NaCHO3-P), while XANES is effective in distinguishing phosphorus bound by metal compounds. In the chronosequence, Ca- and Al-bound phosphorus were derived mainly from primary minerals, whose phosphorus contents decreased within 120 years of moraine weathering and soil development. The content of soil bioavailable phosphorus increased rapidly after 30 years since deglaciation. The increasing phosphorus bioavailability promoted the colonizing and primary succession vegetation. | WU YanHong Jrg PRIETZEL ZHOU Jun BING HaiJian LUO Ji YU Dong SUN ShouQin LIANG JianHong SUN HongYang | 2014 | Science China Earth Sciences2014，57，8： | 6 |
| 3 | Morphology and electrochemical activity of Ru-Ti-Sn ternary-oxide electrodes in 1M NaCl solution显示文摘 | Deng T S Bing J H | 1993 | Electrochem Acta1993，38，15： | 2 |
| 4 | Weight loss in tumour-hearing mice is not associated with changes in resistin gene expression in white adipose tissue 显示文摘 | Gomez-Ambrosi J Zabalegui N Bing C | 2002 | Horm Metab Res2002，34，1112： | 1 |
| 5 | 显示文摘 | Feng Lianghuan Zhang J ingquan Li Bing | 2005 | Thin Solid Films2005，491，12： | 1 |
| 6 | Robust fixture configuration design for sheet metal assembly with laser welding显示文摘 | Li Bing Shiu B W Lau K J | 2003 | Journal of Manufacturing Science and Engineering2003，125，1： | 1 |
| 7 | Integration of microfabricated devices to capillary electrophoresis-electrospray mass spectrometry using a low dead volume connection: application to rapid analysis of proteolytic digests显示文摘 | Li J J Thibault P Bings N H | 1999 | Analytical Chemistry1999，71，15： | 1 |
| 8 | Oxidative processes occurring When pulsed high voltage discharges degrade phenol in aqueous solution显示文摘 | Bing Sun Sato Masayuki Clements J S | 2000 | Journal: Environmental science & technology2000，34，3： | 1 |
| 9 | Detection ofcrack loca- tion and size in structures using wavelet fi - nite element methods 显示文摘 | Li Bing Chen Xuefeng Maetal J X | 2005 | Journal of Sound and Vi - bration2005，285，： | 1 |
| 10 | Oxidative damage causes formation of lipofuscin-like substances in the hippocampus of the senescence-accelerated mouse after kainite treatment显示文摘 | Bing GY J hoo WK | 2002 | Behav Brain Res2002，131，12： | 1 |
| 11 | Relative fiber uniformity between parent and F1 and F2 generations in cotton显示文摘 | Dever J K Bing J R Gannawy | 1992 | Crop Sci1992，32，： | 1 |
| 12 | Acidic polysaccharide of Panax ginseng regulates the mitochondria/caspase-depend- ent apoptotic pathway in radiation-induced damage to the jejunum in mice 显示文摘 | Bing S J Kim M J Ahn G | 2014 | Acta Histochemica2014，116，3： | 1 |
| 13 | Effects of perfusion pressure on myocardial performance, metabolism and wall thinkness ,and compliance显示文摘 | Bing OHL Gasch WH | 1982 | J Thorac Cardiovasc Surg,1982，84，4： | 1 |
| 14 | Low-k damage re- duction using a modified plasma strip process 显示文摘 | RAJESH M BING J STEVE S | 2010 | Solid-State- Technology2010，53，5： | 1 |
| 15 | Physical properties and loading capacity of starch-based microparticles crosslinked with trisodium trime- taphosphate 显示文摘 | Bing Z L Li J W Dong L A | 2009 | Journal of Food Engineering2009，92，： | 1 |
| 16 | Heart muscle viability following hypoxia : protective effect of acidosis显示文摘 | Bing O H Brooks W W Messer J V | 1973 | Science1973，180，： | 1 |
| 17 | Microbubble dynamics visualized in the intact capillary circulation 显示文摘 | Feinstein S B Shah P M Bing R J | 1984 | J Am Coil Cardiol1984，4，3： | 1 |
| 18 | The allocation of risk in PPP/PFI construction pro-jects in the UK显示文摘 | LI Bing AKINTOYE K EDWARDS P J | 2005 | International Journal of ProjectManagement2005，23，1： | 1 |
| 19 | Oxidative processes occurring when pulsed high voltage discharges degrade phenol in aqueous solution显示文摘 | Bing Sun Sato Masayuki Clements J S | | 0，，03： | 1 |
| 20 | Different genotypes of nephropathogenic infectious bronchitis viruses co-circulating in chicken population in China显示文摘 | BING G X LIU X PU J | 2007 | Virus Genes2007，35，2： | 1 |
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