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| 1 | Stocking density ef- fects on broiler welfare: identifying sensitive ranges for different indicators显示文摘 | Buijs S Keeling L Rettenbacher S | 2009 | Poultry Science2009，88，8： | 1 |
| 2 | Translocation (12;22)(p13;q1 1) in myeloproliferative disorders results in fusion of the ETS-like TEL gene on 12p13 to the MN1 gene on 22q11显示文摘 | Buijs A Sherr S van Banl S | 1995 | Oneogene1995，10，： | 1 |
| 3 | The influence of stock- ing density on broiler chicken bone quality and fluctuat- ing a symmetry显示文摘 | BUIJS S VAN P E VAN D S | 2012 | Poultry Science2012，91，8： | 1 |
| 4 | Concentration-dependency of beta-lactam-indueed filament formation in Gram-negative bacteria显示文摘 | Buijs J Dofferhoff A S Mouton J W | 2008 | Clin Microbiol Infect2008，14，4： | 1 |
| 5 | Fatigue and relating factors in high-risk breast cancer patients treated with adjuvant standard or high-dose chemotherapy: a longitudinal study 显示文摘 | Nieboer P Buijs C Rodenhuis S | 2005 | J Clin Oncol2005，23，33： | 1 |
| 6 | TEL/AML1 fusion resulting from a cryptic t (12;21)is the most common genetic lesion in pediatric ALL and defines a subgroup of patients with an excellent prognosis 显示文摘 | SHURTLE S A BUIJS A BEHM F G | 1995 | Leukemia1995，9，： | 1 |
| 7 | Transloeation(12;22)(p13;q11)in myeloproliferative disorders results in fusion of the ETS-like TEL gene on 12p13 to the MN1 gene on 22q11显示文摘 | Buijs A Sherr S van Baal S | 1995 | Oncogene1995，10，8： | 1 |
| 8 | Behavior of domestic fowl in anticipation of positive and negative stimuli 显示文摘 | Zimmerman P H Buijs S A F Bolhuis J E | 2011 | Animal Behavior2011，81，3： | 1 |
| 9 | Protection of bovine enamel and dentine by chlorhexidine and fluoride varnishes in a bacterial demineralization model显示文摘 | Van Loveren C Buijs JF Buij s MJ | 1996 | Caries Res1996，30，1： | 1 |
| 10 | Cominuous administration of PBP - 2 - and PBP - 3 - specific beta - lactams causes higher cytokine responses in murine Pseudomonas aeruginosa and Escherichia coli sepsis 显示文摘 | BUIJS J DOFFERHOFF A S MOUTON J W | 2007 | J Antimicrob Chemother2007，59，5： | 1 |
| 11 | Concentration-dependency of beta-laetam-indueed filament formation in Gram-negative bacteria 显示文摘 | Buijs J Dofferhoff A S Mouton J W | 2008 | Clin Microbiol Infect2008，14，4： | 1 |
| 12 | Stocking density effects on broiler welfare:identifying sensitive ranges for different indicators 显示文摘 | BUIJS S KEELING L RETTENBACHER S | 2009 | Poultry Science2009，88，8： | 1 |
| 13 | The influence of stocking density on broiler chick-en bone quality and fluctuating asymmetry 显示文摘 | BUIJS S VAN POUCKE E VAN DONGEN S etal | 2012 | PoultSci2012，91，8： | 1 |
| 14 | TEL/ AML1 fusion resulting from a cryptic t(12;21) is the most common genetic lesion in pediatric ALL and defines a subgroup of patients with an excellent prognosis显示文摘 | SHURTLE S A BUIJS A BEHM F G | 1995 | Leukemia1995，9，： | 1 |
| 15 | Mammalian Cry1 and Cry2 are essential for maintenance of circadian rhythms显示文摘 | van der Horst GT Muijtjens M Kobayashi K Takano R Kanno S Takao M de Wit J Verkerk A Eker AP van Leenen D Buijs R Bootsma D Hoeijmakers JH Yasui A | | 0，，6728： | 1 |
| 16 | Concentration-dependency of fl-lactam-indueed Filament forma- tion in Gram-negative Bacteria 显示文摘 | BUIJS J DOFFERHOFF A S M MOUTON J W | 2008 | Clin Microbiol Infect2008，14，4： | 1 |
| 17 | Novel multivalent design of a monoclonal antibody improves binding strength to soluble aggregates of amyloid beta显示文摘Background:Amyloid-β(Aβ)immunotherapy is a promising therapeutic strategy in the fght against Alzheimer’s disease(AD).A number of monoclonal antibodies have entered clinical trials for AD.Some of them have failed due to the lack of efcacy or side-efects,two antibodies are currently in phase 3,and one has been approved by FDA.The soluble intermediate aggregated species of Aβ,termed oligomers and protofbrils,are believed to be key pathogenic forms,responsible for synaptic and neuronal degeneration in AD.Therefore,antibodies that can strongly and selectively bind to these soluble intermediate aggregates are of great diagnostic and therapeutic interest.Methods:We designed and recombinantly produced a hexavalent antibody based on mAb158,an Aβprotofbrilselective antibody.The humanized version of mAb158,lecanemab(BAN2401),is currently in phase 3 clinical trials for the treatment of AD.The new designs involved recombinantly fusing single-chain fragment variables to the N-terminal ends of mAb158 antibody.Real-time interaction analysis with LigandTracer and surface plasmon resonance were used to evaluate the kinetic binding properties of the generated antibodies to Aβprotofbrils.Diferent ELISA setups were applied to demonstrate the binding strength of the hexavalent antibody to Aβaggregates of diferent sizes.Finally,the ability of the antibodies to protect cells from Aβ-induced efects was evaluated by MTT assay.Results:Using real-time interaction analysis with LigandTracer,the hexavalent design promoted a 40-times enhanced binding with avidity to protofbrils,and most of the added binding strength was attributed to the reduced rate of dissociation.Furthermore,ELISA experiments demonstrated that the hexavalent design also had strong binding to small oligomers,while retaining weak and intermediate binding to monomers and insoluble fbrils.The hexavalent antibody also reduced cell death induced by a mixture of soluble Aβaggregates.Conclusion:We provide a new antibody design with increased valency to promote binding avidity to an enhanced range of sizes of Aβaggregates.This approach should be general and work for any aggregated protein or repetitive target. | Fadi Rofo Jos Buijs Ronny Falk Ken Honek Lars Lannfelt Anna M.Lilja Nicole G.Metzendorf Tobias Gustavsson Dag Sehlin Linda Söderberg Greta Hultqvist | 2021 | Translational Neurodegeneration2021，10，3： | 0 |
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