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| 1 | Bacteriocin from Bacillus subtilis as a novel drug against diabetic foot ulcer bacterial pathogens显示文摘Objective:To isolate and identify Bacillus subtilis(B.subtilis)from soil and to characterize and partially purify the bacteriocin.To evaluate the antimicrobial activity against four diabetic foot ulcer bacterial pathogens.Methods:Genotypic identification was done based on Sergey's manual of systemic bacteriology.Antimicrobial susceptibility test was done by Kirby-Bauer disc diffusion method.Colonies were identified by colony morphology and biochemical characterization and also compared with MTCC 121 strain.Further identification was done by 16S rRNA sequencing.Inhibitory activities of partially purified bacteriocin on all the DFU isolates were done by agar well diffusion method.The strain was identified to produce bacteriocin by stab overlay assay.Bacteriocin was extracted by organic solvent extraction using chloroform,further purified by HPI.C and physical,and chemical characterization was performed.Results:The four isolates showed high level of resistance to amoxyclav and sensitivity to ciprofloxacin.HPLC purification revealed that the extracts are bacteriocin.The phylogenetic tree analysis results showed that the isolate was 99%related to B.subtilis BSF01.The results reveled activity to all the four isolates and high level of activity was seen in case of Klebsiella sp.Conclusions:Partially purified bacteriocin was found to have antimicrobial activity against the four diabetic foot ulcer bacterial pathogens,which can thus be applied as a better drug molecule on further studies.The strain B.subtilis are found to be safe for use and these antimicrobial peptides can be used as an antimicrobial in humans to treat DFU bacterial pathogens. | Baby Joseph Berlina Dhas Vimalin Hena Justin Raj | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，12： | 2 |
| 2 | Growing seasons of Nordic mountain birch in northernmost Europe as indicated by long-term field studies and analyses of satellite images显示文摘 | E. Shutova F. E. Wielgolaski S. R. Karlsen O. Makarova N. Berlina T. Filimonova E. Haraldsson P. E. Aspholm L. Fl? K. A. H?gda | | 0，，： | 1 |
| 3 | Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk显示文摘 | Berlina A N Taranovan N A Zherdev A V | 2013 | Analytical and Bioanalytical Chemistry2013，405，14： | 1 |
| 4 | Glyceconjugate profiling of primary melanoma and its sentinel node and distant metastases:Implications for diagnosis and pathophysiology of metastases显示文摘 | THIESA BERLINA BRUNNERG | 2007 | Cancer Lett2007，248，1： | 1 |
| 5 | TAFI gene haplotypes,TAFI plasma levels and future risk of coronary heart disease: the PRIME Study显示文摘 | Morange PE Tregouet DA Berlina RM | 2005 | Thromb Haemost2005，3，7： | 1 |
| 6 | Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk显示文摘 | Anna N. Berlina Nadezhda A. Taranova Anatoly V. Zherdev Yuri Y. Vengerov Boris B. Dzantiev | 2013 | Analytical and Bioanalytical Chemistry2013，，14： | 1 |
| 7 | Vegetation changes in semi-natural meadows with unchanged management in southern Sweden, 1965 -1990 显示文摘 | Gudrun A I Berlina Anna-Carin Linusson E Gunilla A Olsson | 2000 | Acta Oecologica2000，21，2： | 1 |
| 8 | Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk显示文摘 | Anna N. Berlina Nadezhda A. Taranova Anatoly V. Zherdev Yuri Y. Vengerov Boris B. Dzantiev | 2013 | Analytical and Bioanalytical Chemistry2013，，14： | 1 |
| 9 | Advantages of soybean peroxidase over horseradish peroxidase as the enzyme label in chemiluminescent enzyme-linked immunosorbent assay of sulfamethoxypyridazine显示文摘 | Sakharov I Y Berlina A N Zherdev A V | 2010 | Journal of Agricultural and Food Chemistry2010，58，6： | 1 |
| 10 | 里斯本的魔法空间显示文摘“陆地之终,海洋之始。”诗人曾赋予了里斯本如画的描述。清晨,我在第一缕阳光的催促下起床,站在阳台上欣赏还未苏醒的城市的景色,沉静的海在一片红屋顶的远端浮动,泛着粼粼的波光,如同一幅文艺复兴的画作。 | Berlina | 2014 | 旅游世界2014，，4： | 0 |
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