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| 1 | Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (KKU-100)显示文摘AIM To establish and dharacterize a nev cholangiocarcinoma cell line from a patient living in the Opisthorchis viverrini (O. viverrini) endemic area of Northeast Thailand.METHODS: Fresh liver biopsy and bile specimens were obtained from a 65-year-old Thai woman with cholangiocarcinoma of the porta hepatis. After digestion, the cells were cultured in Ham's F12 media. The established cell line was then characterized for growth kinetics, cell morphology, imm unocytochemistry and cytogenetics. Tumorigenicity of the cell line was determined by heterotransplanting in nude mice. RESULTS: The primary tumor was a poorly differentiated tubular adenocarcinoma. Examination of the bile revealed malignant cells with O. viverrini eggs. The cholangiocarcinoma cell line KKU-100 was established 4 mo after the primary culture-population doubling time was 72 h. KKU-100 possesses compact and polygonal-shapedepithelial cells. Immunocytochemically, this cell line exhibited cytokeratin, EMA, CEA, and CA125, but not α-fetoprotein (AFP), CA19-9, desmin, c-met, or p53. Such protein expressions parallel those of the primary tumor. Cytogenetic analysis identified aneuploidy karyotypes with a modal chromosome number of 78 and marked chromosomal structural changes. Inoculation of KKU-100 cells into nude mice produced a transplantable, poorly differentiated aden-ocarcinoma, similar to the original tumor.CONCLUSION: KKJ-100 is the first egg-proven, Opisthorchis- associated cholangiocarcinoma cell line, which should prove useful for further investigations of the tumor biology of this cancer. | Banchob Sripa Saman Leungwattanawanit Takayuki Nitta Chaisiri Wongkham Vajarabhongsa Bhudhisawasdi Anucha Puapairoj Chongrak Sripa Masanao Miwa | 2005 | World Journal of Gastroenterology2005，11，22： | 4 |
| 2 | Optimization of resourceprovisioning cost in cloud computing 显示文摘 | Chaisiri S Lee B S Niyato D | 2012 | IEEE Trans onServices Computing2012，5，2： | 1 |
| 3 | Cooperative virtual machine management in smart grid environment 显示文摘 | Kaewpuang R Chaisiri S Niyato D | 2014 | IEEE Transactions on Services Computing2014，7，4： | 1 |
| 4 | Genetic and environmental determinants of risk for cholangiocarcinoma in Thailand显示文摘Cholangiocarcinoma(CCA) is a difficult cancer to diagnose in the early stage and to treat by curative resec-tion. The incidence of CCA in the northeast of Thailand is the highest in the world. To make progress in detecting a high risk group and in the prevention and detection of CCA, we have been analyzing the risk factors for CCA. Although liver fluke infection is known to be a risk factor, there are patients who are not infected with the liver fluke and not all people infected with the liver fluke will suffer from the disease. Therefore, it is of the utmost importance to analyze the risk factors and the mechanism to prevent the disease and also to detect the disease in its early stage to save patients' lives. Through collaboration among Thai and Japanese researchers, we analyzed the genetic and environmental determinants of risks for CCA. Also, we have been trying to develop methods to detect the disease in a non-invasive way. Without repeating findings reported in various reviews on CCA, we will first discuss the environmental and genetic determinants of the risks for CCA. Second, we will discuss the properties of CCA, including the etiological agents and the mechanism of cholangiocarcinogenesis, and finally, we will discuss future approaches to prevent and cure CCA from the standpoint of evidence-based medicine. We will discuss these points by including the data from our laboratories. We would like to emphasize the importance of the genetic data, especially whole genome approaches, to understand the properties of CCA, to find a high risk population for CCA and to develop effective preventative methods to stop the carcinogenic steps toward CCA in the near future. In addition, it is of the upmost importance to develop a non-invasive, specific and sensitive method to detect CCA in its early stage for the application of modern medical approaches to help patients with CCA. | Masanao Miwa Satoshi Honjo Gyokukou You Masakazu Tanaka Kazuhiko Uchida Petcharin Srivatanakul Thiravud Khuhaprema Watcharin Loilome Anchalee Techasen Chaisiri Wongkham Temduang Limpaiboon Puangrat Yongvanit Sopit Wongkham | 2014 | World Journal of Gastrointestinal Pathophysiology2014，5，4： | 1 |
| 5 | Decreased expression of galectin-3 is associated with metastatic potential of liver fluke-associated cholangiocarcinoma显示文摘 | Mutita Junking Chaisiri Wongkham Banchob Sripa Kanlayanee Sawanyawisuth Norie Araki Sopit Wongkham | 2008 | European Journal of Cancer2008，，4： | 1 |
| 6 | Optimization of resource provisioning cost in cloud computing 显示文摘 | CHAISIRI S LEE B S NIYATO D | 2012 | IEEE Transactions on Services Computing2012，5，2： | 1 |
| 7 | Incidence and risk factors for tenofovir-associated renal function decline among Thai HIV-infected patients with low-body weight显示文摘 | Chaisiri K Bowonwatanuwong C Kasettratat N | 2010 | Curr HIV Res2010，8，7： | 1 |
| 8 | Optimization of resource provisioning cost in cloud computing 显示文摘 | CHAISIRI S LEE B NIYATO D | 2011 | IEEE Transactions on Services Computing2011，99，7： | 1 |
| 9 | Optimization of Resource Provisioning Cost in Cloud Computing显示文摘 | Sivadon Chaisiri Bu -Sung Lee Dusit Niyato | 2012 | IEEE Trans- actions on Services Computing2012，5，2： | 1 |
| 10 | Serodiagnosis of humen Fascioliasis by a cystatin capture enzyme-linked immuosorbent assay with recombinant Fasciola gigantica Cathepsin L antigen显示文摘 | Chairrat Tantrawatpan Wanchai Maleewong Chaisiri Wongkham | 2005 | Am J Trop Med Hyg2005，72，1： | 1 |
| 11 | Optimization of Resource Provisioning Cost in Cloud Computing显示文摘 | Chaisiri S Lee A S Niyato D | 2011 | IEEE Transactions on Services Computing2011，5，2： | 1 |
| 12 | Cooperative Virtual Machine Management in Smart Grid Environment显示文摘 | Kaewpuang R Chaisiri S Niyato D | 2014 | IEEE Transactions on Services Computing2014，7，4： | 1 |
| 13 | Optimization of resource provisioning cost in cloud computing显示文摘 | CHAISIRI S LEE B NIYATO D | | 0，，07： | 1 |
| 14 | Roles and Mechanisms of β-Thymosins in Cell Migration and Cancer Metastasis: An Update显示文摘 | Sirinapa Sribenja Sopit Wongkham Chaisiri Wongkham Qizhi Yao Changyi Chen | 2013 | Cancer Investigation2013，，2： | 1 |
| 15 | Optimization of resource provisioning cost in cloud computing显示文摘 | Chaisiri S Lee B Niyato D | 2011 | IEEE Transactions on Services Computing2011，99，7： | 1 |
| 16 | CA ‐S27: A novel Lewis a associated carbohydrate epitope is diagnostic and prognostic for cholangiocarcinoma显示文摘 | Atit Silsirivanit Norie Araki Chaisiri Wongkham Kulthida Vaeteewoottacharn Chawalit Pairojkul Kazuhiko Kuwahara Yoshiki Narimatsu Hiromichi Sawaki Hisashi Narimatsu Seiji Okada Nobuo Sakaguchi Sopit Wongkham | 2013 | Cancer Sci2013，，10： | 1 |
| 17 | Efficacy and safety of different dual antiplatelet strategies in patients undergoing percutaneous coronary intervention:A systematic review and network meta-analysis显示文摘Background:Dual antiplatelet therapy(DAPT)is key for preventing ischaemic events post-percutaneous coronary intervention(PCI).Various DAPT modifications like the shortened duration or P2Y12 inhibitor(P2Y12i)de-escalation are implemented to reduce bleeding risk.However,these strategies lack direct comparative studies.This study aimed to assess the efficacy and safety of such DAPT strategies,including de-escalated and short DAPT,in patients undergoing PCI.Methods:We searched PubMed,Embase,Cochrane Central Register of Controlled Trials,and ClinicalTrials.gov databases for relevant randomized controlled trials(RCTs).We performed a network meta-analysis(NMA)to estimate risk ratios(RRs)and 95%confidence intervals(CIs).The primary efficacy endpoint was major adverse cardiac events(MACEs),and the primary safety endpoint was major bleeding.Secondary endpoints included individual components of MACEs and net adverse clinical events(NACEs).Results:A total of 17 RCTs comprising 53,156 patients(median age,62.0 years,24.8%female)were included.NMA suggested that de-escalation DAPT was associated with a significantly lower risk of MACEs(risk ratio[RR]=0.79,95%confidence interval[CI]=0.64-0.98),bleeding(RR=0.63,95%CI=0.49-0.82),and NACEs(RR=0.69,95%CI=0.60-0.79)compared with standard DAPT.Short DAPT followed by P2Y12i monotherapy exhibited a significantly decreased risk of major bleeding(RR=0.63,95%CI=0.46-0.86)compared with standard DAPT.Conclusions:De-escalation DAPT was the most effective strategy for preventing the risk of MACEs without increasing bleeding events,while short DAPT followed by P2Y12i monotherapy was the most effective strategy for reducing the risk of bleeding among patients undergoing PCI. | Yuttana Wongsalap Kirati Kengkla Preyanate Wilairat Khemanat Ratworawong Surasak Saokaew Chaisiri Wanlapakorn | 2023 | Chronic Diseases and Translational Medicine2023，9，4： | 0 |
| 18 | Biofilm formation in trimethoprim/sulfamethoxazole-susceptible and trimethoprim/sulfamethoxazoleresistant uropathogenic Escherichia coli显示文摘Objective: To compare bioi lm formation in trimethoprim/sulfamethoxazole(SXT)-susceptible Escherichia coli(E. coli)(SSEC) and SXT-resistant E. coli(SREC) isolated from patients with urinary tract infections, and study the motile ability and physical characteristics of the isolates.Methods: A total of 74 E. coli isolates were tested for antimicrobial susceptibility with the disc diffusion assay. Based on the SXT-susceptibility test, the E. coli isolates were divided into SSEC(N = 30) and SREC(N = 44) groups. All E. coli isolates were examined for motile ability by using a motility test medium, and for checking bioi lm formation a scanning electron microscope was used. Bacterial colony size was measured with a vernier caliper and bacterial cell length was measured under a light microscope. The bacterial growth rate was studied by plotting the cell growth(absorbance) versus the incubation time. Results: The frequencies of non-motility and biofilm formation in the SREC group were signii cantly higher than that in the SSEC group(P < 0.01). The SREC bacterial cell length was shorter than that in the SSEC group [(1.35 ± 0.05) vs.(1.53 ± 0.05) μm, P < 0.05)], whereas the bacterial colony size and mid-log phase of the growth curve were not signii cantly dif erent. Conclusions: The present study indicated that bioi lm formation and phenotypic change of uropathogenic E. coli can be attributed to the mechanism of E. coli SXT resistance. | Nitis Smanthong Ratree Tavichakorntrakool Phitsamai Saisud Vitoon Prasongwatana Pipat Sribenjalux Aroonlug Lulitanond Orathai Tunkamnerdthai Chaisiri Wongkham Patcharee Boonsiri | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，6： | 0 |
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