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| 1 | Characterizati0n,development and exploitation of EST-derived microsatellites in Gossypium raimondii Ulbrich显示文摘Microsatellite DNA or simple sequence repeats (SSRs) can be derived from expressed se- quence tags (ESTs). These markers are important for gene mapping as well as marker-assisted selection (MAS). To develop EST-SSRs for cotton gene map- ping, we selected and characterized functional markers in Gossypium raimondii, which consisted of 58906 non-redundant EST sequences from NCBI. Among them there were 2620 microsatellite se- quences containing 2818 EST-SSRs, which amoun- ted to 4.45% of the non-redundant starting sequence population. This incidence was equivalent to one EST-SSR in every 14.8 kb of G. raimondii genetic material. Among the different motifs ranging from 1 to 6 bp, trinucleotide repeats were most abundant (38.31%), followed by dinucleotide repeats (24.09%) and mononucleotide repeats (23.35%). Among all identified motif types, A/T had the highest frequency (18.67%), followed by AT/TA (14.83%). Among the compound motifs, tandem trinucleotides occurred with the highest frequency (48.65%). In all, we identi- fied 1554 EST-SSRs primer pair sequences. 300 of them were randomly selected to screen the poly- morphisms between the mapping parents G· hirsutum acc. TM-1 and G· barbadense cv. Hai7124, to con- struct linkage groups in cultivated allotetraploid cot- ton. Among them, 129 (43%) primer pairs were found to have polymorphisms. Using these EST-SSRs we can compare EST-SSR distributions among different cotton species and various chromosomal locations. | WANG Changbiao GUO Wangzhen CAI Caiping ZHANG Tianzhen | 2006 | Chinese Science Bulletin2006，51，5： | 21 |
| 2 | A preliminary analysis of genome structure and composition in Gossypium hirsutum显示文摘 | Guo Wangzhen Cai Caiping Wang Changbiao | 2008 | BMC Genomics2008，9，： | 1 |
| 3 | Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton显示文摘 | Zhiguo Han Changbiao Wang Xianliang Song Wangzhen Guo Jinying Gou Chunhong Li Xiaoya Chen Tianzhen Zhang | 2006 | Theoretical and Applied Genetics2006，，3： | 1 |
| 4 | Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton显示文摘 | Zhiguo Han Changbiao Wang Xianliang Song Wangzhen Guo Jinying Gou Chunhong Li Xiaoya Chen Tianzhen Zhang | 2006 | Theoretical and Applied Genetics2006，，3： | 1 |
| 5 | Characterization of eight Bacillus thuringiensis isolates originated from fecal samples of Fuzhou Zoo and Fuzhou Panda Center显示文摘 | Changbiao Wu Lili Wu Lingling Zhang Ivan Gelbi? Lei Xu Xiong Guan | 2014 | Journal of Asia-Pacific Entomology2014，，： | 1 |
| 6 | Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton显示文摘 | Zhiguo Han Changbiao Wang Xianliang Song Wangzhen Guo Jinying Gou Chunhong Li Xiaoya Chen Tianzhen Zhang | 2006 | Theoretical and Applied Genetics2006，，3： | 1 |
| 7 | Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton显示文摘 | Zhiguo Han Changbiao Wang Xianliang Song Wangzhen Guo Jinying Gou Chunhong Li Xiaoya Chen Tianzhen Zhang | 2006 | Theoretical and Applied Genetics2006，，3： | 1 |
| 8 | Functional Characterization and Crystal Structure of the Type Ei Peptidyl Carrier Protein ColAia in Collismydns Biosynthesis显示文摘Summary of main observation and conclusion Collismycins(COLs)are antibiotics characterized by a 2,2'-bipyridine(2,2'-BP)core composed of a trisubstituted ring A and an unmodified ring B.The 2,2'-BP core,which possesses metal-chelating ability and plays key roles in various biological activities of COLs,is biosynthesized by a nonribosomal peptide synthetase(NRPS)-polyketide synthase(PKS)hybrid machinery.The starter module of the NRPS-PKS hybrid machinery consists of a type II peptidyl carrier protein(PCP)ColA1a and an adenylation protein ColA1b.We here report the functional characterization of ColAia and ColAlb in vitro,confirming their functions in selection and loading of picolinic acid(PA),instead of normal amino acid substrates,as the origin of ring B in COLs.The 2.1 A crystal structure of ColAia was solved,revealing structural features including the additional helices ala,alb and missing helix a3,which may reflect unique interactions of ColAia with other NRPS-PKS proteins/domains or substrate.Primary and tertiary structural comparison of ColAia with other PCPs revealed the structural basis for their typical a-helical bundle,providing a better understanding of the structural flexibility of PCPs.These results facilitate the starter module engineering for the generation of COL derivatives with ring B modifications in the future. | Xueyang Ma Guiyang Wang Tan Liu Changbiao Chi Zhongyi Zhang Donghui Yang Wen Liu Ming Ma | 2020 | Chinese Journal of Chemistry2020，38，9： | 1 |
| 9 | Application of Attribute Reduction to Well Logging显示文摘很好记载数据的数量指数地正在增加,因此从记载的数据库提取有用信息或归属的方法在记载解释正在变得很重要。那么,记载属性减小的方法基于一个不平的集合被介绍,即,首先决定信息表格的核心,然后计算每个属性的意义,并且最后获得相对减小表格。申请结果证明属性减小的方法是可行的并且能被用于优化记载属性,并且减少冗余的记载信息在很大程度上。 | Li Changbiao Song Jianping Xia Kewen | 2005 | Petroleum Science2005，2，4： | 0 |
| 10 | Enzymatic Synthesis of a Diastereomer of Neoabyssomicin Derivative Using the Diels-Alderase AbyU显示文摘Main observation and conclusion The enzyme AbyU catalyses a Diels-Alder(DA)reaction during abyssomicin C biosynthesis.In this study,AbyU is shown to convert the native substrate of another Diels-Alderase(DAase),AbmU,to a new abyssomicin derivative,abyssomicin 7. | Wenjuan Ding Changbiao Chi Xiaoyi Wei Changli Sun Jiajia Tu Ming Ma Qinglian Li Jianhua Ju | 2021 | Chinese Journal of Chemistry2021，39，7： | 0 |
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