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| 1 | Hyaluronic acid facilitates bone repair effects of calcium phosphate cement by accelerating osteogenic expression显示文摘Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects.However the clinical application of CPC is still not satisfied by its poor cohesiveness and mechanical properties,in particular its osteoinductivity.Hyaluronic acid reinforced calcium phosphate cements(HA/CPC)showed extroadinary potential not only enhancing the compressive strength of the cements but also significantly increasing its osteoinductivity.In our study,the compressive strength of HA/CPC increased significantly when the cement was added 1%hyaluronic acid(denoted as 1-HA/CPC).In the meantime,hyaluronic acid obviously promoted ALP activity,osteogenic related protein and mRNA expression of hBMSCs(human bone marrow mesenchymal stem cells)in vitro,cement group of HA/CPC with 4%hyaluronic acid adding(denoted as 4-HA/CPC)showed optimal enhancement in hBMSCs differentiation.After being implanted in rat tibial defects,4-HA/CPC group exhibited better bone repair ability and bone growth promoting factors,comparing to pure CPC and 1-HA/CPC groups.The underlying biological mechanism of this stimulation for HA/CPC may be on account of higher osteogenic promoting factors secretion and osteogenic genes expression with hyaluronic acid incorporation.These results indicate that hyaluronic acid is a highly anticipated additive to improve physicochemical properties and osteoinductivity performance of CPCs for minimally invasive healing of bone defects. | Xu Cui Chengcheng Huang Zhizhen Chen Meng Zhang Chunyu Liu Kun Su Jianyun Wang Li Li Renxian Wang Bing Li Dafu Chen Changshun Ruan Deping Wang William W.Lu Haobo Pan | 2021 | Bioactive Materials2021，6，11： | 6 |
| 2 | Strontium modulates osteogenic activity of bone cement composed of bioactive borosilicate glass particles by activating Wnt/β-catenin signaling pathway显示文摘There is a need for synthetic grafts to reconstruct large bone defects using minimal invasive surgery.Our previous study showed that incorporation of Sr into bioactive borate glass cement enhanced the osteogenic capacity in vivo.However,the amount of Sr in the cement to provide an optimal combination of physicochemical properties and capacity to stimulate bone regeneration and the underlying molecular mechanism of this stimulation is yet to be determined.In this study,bone cements composed of bioactive borosilicate glass particles substituted with varying amounts of Sr(0 mol%to 12 mol%SrO)were created and evaluated in vitro and in vivo.The setting time of the cement increased with Sr substitution of the glass.Upon immersion in PBS,the cement degraded and converted more slowly to HA(hydroxyapatite)with increasing Sr substitution.The released Sr2+modulated the proliferation,differentiation,and mineralization of hBMSCs(human bone marrow mesenchymal stem cells)in vitro.Osteogenic characteristics were optimally enhanced with cement(designated BG6Sr)composed of particles substituted with 6mol%SrO.When implanted in rabbit femoral condyle defects,BG6Sr cement supported better peri-implant bone formation and bone-implant contact,comparing to cements substituted with 0mol%or 9mol%SrO.The underlying mechanism is involved in the activation of Wnt/β-catenin signaling pathway in osteogenic differentiation of hBMSCs.These results indicate that BG6Sr cement has a promising combination of physicochemical properties and biological performance for minimally invasive healing of bone defects. | Xu Cui Yadong Zhang Jianyun Wang Chengcheng Huang Yudong Wang Hongsheng Yang Wenlong Liu Ting Wang Deping Wang Guocheng Wang Changshun Ruan Dafu Chen William W.Lu Wenhai Huang Mohamed N.Rahaman Haobo Pan | 2020 | Bioactive Materials2020，5，2： | 3 |
| 3 | Stepwise 3D-spatio-temporal magnesium cationic niche: Nanocomposite scaffold mediated microenvironment for modulating intramembranous ossification显示文摘The fate of cells and subsequent bone regeneration is highly correlated with temporospatial coordination of chemical,biological,or physical cues within a local tissue microenvironment.Deeper understanding of how mammalian cells react to local tissue microenvironment is paramount important when designing next generation of biomaterials for tissue engineering.This study aims to investigate that the regulation of magnesium cationic(Mg^2+)tissue microenvironment is able to convince early-stage bone regeneration and its mechanism undergoes intramembranous ossification.It was discovered that moderate Mg^2+content niche(~4.11 mM)led to superior bone regeneration,while Mg^2+-free and strong Mg^2+content(~16.44 mM)discouraged cell adhesion,proliferation and osteogenic differentiation,thereby bone formation was rarely found.When magnesium ions diffused into free Mg zone from concentrated zone in late time point,new bone formation on free Mg zone became significant through intramembranous ossification.This study successfully demonstrates that magnesium cationic microenvironment serves as an effective biochemical cue and is able to modulate the process of bony tissue regeneration.The knowledge of how a Mg^2+cationic microenvironment intertwines with cells and subsequent bone formation gained from this study may provide a new insight to develop the next generation of tissuerepairing biomaterials. | Jie Shen Bo Chen Xinyun Zhai Wei Qiao Shuilin Wu Xuanyong Liu Ying Zhao Changshun Ruan Haobo Pan Paul K.Chu Kenneth M.C.Cheung Kelvin W.K.Yeung | 2021 | Bioactive Materials2021，6，2： | 2 |
| 4 | Fractal Design Boosts Extrusion-Based 3D Printing of Bone-Mimicking Radial-Gradient Scaffolds显示文摘Although extrusion-based three-dimensional(EB-3D)printing technique has been widely used in the complex fabrication of bone tissue-engineered scaffolds,a natural bone-like radial-gradient scaffold by this processing method is of huge challenge and still unmet.Inspired by a typical fractal structure of Koch snowflake,for the first time,a fractal-like porous scaffold with a controllable hierarchical gradient in the radial direction is presented via fractal design and then implemented by EB-3D printing.This radial-gradient structure successfully mimics the radially gradual decrease in porosity of natural bone from cancellous bone to cortical bone.First,we create a design-to-fabrication workflow with embedding the graded data on basis of fractal design into digital processing to instruct the extrusion process of fractal-like scaffolds.Further,by a combination of suitable extruded inks,a series of bone-mimicking scaffolds with a 3-iteration fractal-like structure are fabricated to demonstrate their superiority,including radial porosity,mechanical property,and permeability.This study showcases a robust strategy to overcome the limitations of conventional EB-3D printers for the design and fabrication of functionally graded scaffolds,showing great potential in bone tissue engineering. | Huawei Qu Zhenyu Han Zhigang Chen Lan Tang Chongjian Gao Kaizheng Liu Haobo Pan Hongya Fu Changshun Ruan | 2021 | Research2021，，1： | 2 |
| 5 | RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer显示文摘Objective:RECQL4(a member of the RECQ helicase family)upregulation has been reported to be associated with tumor progression in several malignancies.However,whether RECQL4 sustains esophageal squamous cell carcinoma(ESCC)has not been elucidated.In this study,we determined the functional role for RECQL4 in ESCC progression.Methods:RECQL4 expression in clinical samples of ESCC was examined by immunohistochemistry.Cell proliferation,cellular senescence,the epithelial-mesenchymal transition(EMT),DNA damage,and reactive oxygen species in ESCC cell lines with RECQL4 depletion or overexpression were analyzed.The levels of proteins involved in the DNA damage response(DDR),cell cycle progression,survival,and the EMT were determined by Western blot analyses.Results:RECQL4 was highly expressed in tumor tissues when compared to adjacent non-tumor tissues in ESCC(P<0.001)and positively correlated with poor differentiation(P=0.011),enhanced invasion(P=0.033),and metastasis(P=0.048).RECQL4 was positively associated with proliferation and migration in ESCC cells.Depletion of RECQL4 also inhibited growth of tumor xenografts in vivo.RECQL4 depletion induced G0/G1 phase arrest and cellular senescence.Importantly,the levels of DNA damage and reactive oxygen species were increased when RECQL4 was depleted.DDR,as measured by the activation of ATM,ATR,CHK1,and CHK2,was impaired.RECQL4 was also shown to promote the activation of AKT,ERK,and NF-k B in ESCC cells.Conclusions:The results indicated that RECQL4 was highly expressed in ESCC and played critical roles in the regulation of DDR,redox homeostasis,and cell survival. | Guosheng Lyu Peng Su Xiaohe Hao Shiming Chen Shuai Ren Zixiao Zhao Yaoqin Gong Qiao Liu Changshun Shao | 2021 | Cancer Biology & Medicine2021，18，1： | 2 |
| 6 | Mutation in CUL4B, Which Encodes a Member of Cullin-RING Ubiquitin Ligase Complex, Causes X-Linked Mental Retardation显示文摘 | Yongxin Zou Qiji Liu Bingxi Chen Xiyu Zhang Chenhong Guo Haibin Zhou Jiangxia Li Guimin Gao Yishou Guo Chuanzhu Yan Jianjun Wei Changshun Shao Yaoqin Gong | 2007 | The American Journal of Human Genetics2007，，3： | 1 |
| 7 | NAD^(+) salvage governs the immunosuppressive capacity of mesenchymal stem cells显示文摘Mesenchymal stem/stromal cells(MSCs)possess robust immunoregulatory functions and are promising therapeutics for inflammatory disorders.This capacity is not innate but is activated or‘licensed’by inflammatory cytokines.The licensing mechanism remains unclear.Here,we examined whether inflammatory cytokines metabolically reprogrammed MSCs to confer this immunoregulatory capacity.In response to stimulation by inflammatory cytokines,MSCs exhibited a dramatic increase in the consumption of glucose,which was accompanied by an enhanced use of nicotinamide adenine dinucleotide(NAD^(+))and increased expression of nicotinamide phosphoribosyltransferase(NAMPT),a central enzyme in the salvage pathway for NAD^(+) production.When NAD^(+) synthesis was blocked by inhibiting or depleting NAMPT,the immunosuppressive function of MSCs induced by inflammatory cytokines was greatly attenuated.Consequently,when NAD^(+) metabolism in MSCs was perturbed,their therapeutic benefit was decreased in mice suffering from inflammatory bowel disease and acute liver injury.Further analysis revealed that NAMPT-driven production of NAD^(+) was critical for the inflammatory cytokine-induced increase in glycolysis in MSCs.Furthermore,the increase in glycolysis led to succinate accumulation in the tricarboxylic acid cycle,which led to hypoxia-inducible factor 1α(HIF-1α)stabilization and subsequently increased the transcription of key glycolytic genes,thereby persistently maintaining glycolytic flux.This study demonstrated that unlike its proinflammatory role in immune cells,NAD^(+) metabolism governs the anti-inflammatory function of MSCs during inflammation. | Jiankai Fang Pengbo Hou Shisong Liu Muqiu Zuo Zhanhong Liu Wangwang Chen Yuyi Han Yanan Li Tingting Wang Chao Feng Peishan Li Changshun Shao Yufang Shi | 2023 | Cellular & Molecular Immunology2023，20，10： | 1 |
| 8 | The under- ground stress design method of coal entry bolt supporting 显示文摘 | Ma Nianjie Hu Changshun Liu Xicai | 1997 | Ground Pressure and Strata Contrel1997，14，34： | 1 |
| 9 | Antibacterial effects and biocompatibility of titanium surfaces with graded silver incorporation in titania nanotubes显示文摘 | Shenglin Mei Huaiyu Wang Wei Wang Liping Tong Haobo Pan Changshun Ruan Qianli Ma Mengyuan Liu Huiling Yang Liang Zhang Yicheng Cheng Yumei Zhang Lingzhou Zhao Paul K. Chu | 2014 | Biomaterials2014，，： | 1 |
| 10 | MiR‐182 overexpression in tumourigenesis of high‐grade serous ovarian carcinoma显示文摘 | Zhaojian Liu Jinsong Liu Miguel F Segura Changshun Shao Peng Lee Yaoqin Gong Eva Hernando Jian‐Jun Wei | 2012 | J Pathol2012，，2： | 1 |
| 11 | Neferine, an alkaloid ingredient in lotus seed embryo, inhibits proliferation of human osteosarcoma cells by promoting p38 MAPK-mediated p21 stabilization显示文摘 | Xiyu Zhang Zhaojian Liu Bing Xu Zhaoliang Sun Yaoqin Gong Changshun Shao | 2011 | 2011 (1-3)2011，，1： | 1 |
| 12 | Two-Layer Information Granulation:Mapping-Equivalence Neighborhood Rough Set and Its Attribute Reduction显示文摘Attribute reduction,as one of the essential applications of the rough set,has attracted extensive attention from scholars.Information granulation is a key step of attribute reduction,and its efficiency has a significant impact on the overall efficiency of attribute reduction.The information granulation of the existing neighborhood rough set models is usually a single layer,and the construction of each information granule needs to search all the samples in the universe,which is inefficient.To fill such gap,a new neighborhood rough set model is proposed,which aims to improve the efficiency of attribute reduction by means of two-layer information granulation.The first layer of information granulation constructs a mapping-equivalence relation that divides the universe into multiple mutually independent mapping-equivalence classes.The second layer of information granulation views each mapping-equivalence class as a sub-universe and then performs neighborhood informa-tion granulation.A model named mapping-equivalence neighborhood rough set model is derived from the strategy of two-layer information granulation.Experimental results show that compared with other neighborhood rough set models,this model can effectively improve the efficiency of attribute reduction and reduce the uncertainty of the system.The strategy provides a new thinking for the exploration of neighborhood rough set models and the study of attribute reduction acceleration problems. | Changshun Liu Yan Liu Jingjing Song Taihua Xu | 2023 | Intelligent Automation & Soft Computing2023，37，8： | 0 |
| 13 | Small-molecule amines:a big role in the regulation of bone homeostasis显示文摘Numerous small-molecule amines(SMAs)play critical roles in maintaining bone homeostasis and promoting bone regeneration regardless of whether they are applied as drugs or biomaterials.On the one hand,SMAs promote bone formation or inhibit bone resorption through the regulation of key molecular signaling pathways in osteoblasts/osteoclasts;on the other hand,owing to their alkaline properties as well as their antioxidant and anti-inflammatory features,most SMAs create a favorable microenvironment for bone homeostasis.However,due to a lack of information on their structure/bioactivity and underlying mechanisms of action,certain SMAs cannot be developed into drugs or biomaterials for bone disease treatment.In this review,we thoroughly summarize the current understanding of SMA effects on bone homeostasis,including descriptions of their classifications,biochemical features,recent research advances in bone biology and related regulatory mechanisms in bone regeneration.In addition,we discuss the challenges and prospects of SMA translational research. | Qian Zhang Jirong Yang Nan Hu Juan Liu Huan Yu Haobo Pan Di Chen Changshun Ruan | 2023 | Bone Research2023，11，3： | 0 |
| 14 | 3D printed porous titanium filled with mineralized UV-responsive chitosan hydrogel promotes cell proliferation and osteogenesis in vitro显示文摘The modification of 3D printed porous titanium(Ti),especially for the internal pore structure,is critical and has received more attention to promoting its osteogenesis for clinical use.Ultra-violet(UV)responsive chitosan(CSMA),as an injectable filling material,was firstly incorporated into porous Ti,and then CSMA was in-situ mineralized by carbon oxide(CO_(2))diffusion(CSMA/CaCO_(3)).Their physical-chemical and biological properties were investigated in vitro.CaCO_(3) crystals within CSMA hydrogels were successfully deposited into pores of porous Ti,which exhibited favorable biocompatibility.Ti implants filled with CSMA/CaCO_(3) promoted adhesion and proliferation of bone mesenchymal stem cells(BMSCs).Moreover,Ti implant filled CSMA/CaCO_(3) hydrogels could increase alkaline phosphatase(ALP)activities,up-regulate osteopontin(OPN)and osteocalcin(OCN)expression levels,and enhance extracellular mineralization.3D printed porous Ti filled with mineralized UV-responsive chitosan hydrogel could promote proliferation and osteogenesis of BMSCs,and have great potential for the modification of porous Ti implants in bone tissue engineering. | Jiazhao Yang Fan Liu Changshun Zhou Hejie Li Gaolin Yang Shiyuan Fang In-Seop Lee Yi Liu Hao Bai Cen Chen | 2023 | Journal of Materials Science & Technology2023，，11： | 0 |
| 15 | Cell-matrix reciprocity in 3D culture models with nonlinear elasticity显示文摘Three-dimensional(3D)matrix models using hydrogels are powerful tools to understand and predict cell behavior.The interactions between the cell and its matrix,however is highly complex:the matrix has a profound effect on basic cell functions but simultaneously,cells are able to actively manipulate the matrix properties.This(mechano)reciprocity between cells and the extracellular matrix(ECM)is central in regulating tissue functions and it is fundamentally important to broadly consider the biomechanical properties of the in vivo ECM when designing in vitro matrix models.This manuscript discusses two commonly used biopolymer networks,i.e.collagen and fibrin gels,and one synthetic polymer network,polyisocyanide gel(PIC),which all possess the characteristic nonlinear mechanics in the biological stress regime.We start from the structure of the materials,then address the uses,advantages,and limitations of each material,to provide a guideline for tissue engineers and biophysicists in utilizing current materials and also designing new materials for 3D cell culture purposes. | Kaizheng Liu Maury Wiendels Hongbo Yuan Changshun Ruan Paul H.J.Kouwer | 2022 | Bioactive Materials2022，7，3： | 0 |
| 16 | Fractal Design Boosts Extrusion-Based 3D Printing of Bone-Mimicking Radial-Gradient Scaffolds显示文摘Although extrusion-based three-dimensional(EB-3D)printing technique has been widely used in the complex fabrication of bone tissue-engineered scaffolds,a natural bone-like radial-gradient scaffold by this processing method is of huge challenge and still unmet.Inspired by a typical fractal structure of Koch snowflake,for the first time,a fractal-like porous scaffold with a controllable hierarchical gradient in the radial direction is presented via fractal design and then implemented by EB-3D printing.This radial-gradient structure successfully mimics the radially gradual decrease in porosity of natural bone from cancellous bone to cortical bone.First,we create a design-to-fabrication workflow with embedding the graded data on basis of fractal design into digital processing to instruct the extrusion process of fractal-like scaffolds.Further,by a combination of suitable extruded inks,a series of bone-mimicking scaffolds with a 3-iteration fractal-like structure are fabricated to demonstrate their superiority,including radial porosity,mechanical property,and permeability.This study showcases a robust strategy to overcome the limitations of conventional EB-3D printers for the design and fabrication of functionally graded scaffolds,showing great potential in bone tissue engineering. | Huawei Qu Zhenyu Han Zhigang Chen Lan Tang Chongjian Gao Kaizheng Liu Haobo Pan Hongya Fu Changshun Ruan | 2022 | Research2022，，1： | 0 |
| 17 | Development and application of high-precision multifunction astronomical plate digitizers in China显示文摘Before charge-coupled device detectors became widely employed in observational astronomy,for more than a hundred years,the main detection method was photography on astronomical glass plates.Recently,in order to preserve these historical data and maintain their usability,the International Astronomical Union has appealed to all countries for global digitization of astronomical plates by developing or adopting advanced digitization technology.Specialized digitizers with high precision and high measuring speed represent key equipment for this task.The Shanghai Astronomical Observatory and the Nishimura Co.,Ltd in Japan cooperated between 2013 and 2016 to develop the first Chinese high-precision astronomical plate digitizer,which was then used for complete digitization of all nighttime-observation astronomical plates in China.Then,in 2019–2021,the Shanghai Astronomical Observatory independently developed new models of plate digitizers that enabled countries such as Uzbekistan and Italy to digitize their astronomical plates.Additionally,a new high-precision and multifunction digitizer was also used to digitize valuable microscope slides from the Shanghai Natural History Museum,providing a successful example of cross-domain application of high-precision digitization technology. | Meiting Yang Yong Yu Liangliang Wang Zhengjun Shang Changshun Liu Lixin Zheng Zhendong Chen Jing Yang Dongmei Da Shan Dong Shiyin Shen Zhenghong Tang Jianhai Zhao | 2024 | Astronomical Techniques and Instruments2024，1，1： | 0 |
| 18 | 3D multiphysic simulations of energy field and material process in radial ultrasonic rolling electrochemical micromachining显示文摘The radial ultrasonic rolling electrochemical micromachining(RUR-EMM)combined rolling electrochemical micromachining(R-EMM)and ultrasonic vibration was studied in this paper.The fundamental understanding of the machining process especially the interaction between multiphysics in the interelectrode gap(IEG)was investigated and discussed by the finite element method.The multiphysics coupling model including flow field model,Joule heating model,material dissolution model and vibration model was built.3D multiphysics simulation based on micro dimples process in RUR-EMM and R-EMM was proposed.Simulation results showed that the electrolyte flowed into and out IEG periodically,gas bubbles were easy to squeeze out and the gas void fraction deceased about 16%to 54%,the maximum current density increased by 1.36 times in RUR-EMM than in R-EMM in one vibration period of time.And application of the ultrasonic vibration increased the electrolyte temperature about 1.3–4.4%in IEG.Verification experiments of the micro dimple process denoted better corrosion consistency of array dimples in RUR-EMM,there was no island at the micro dimple bottom which always formed in R-EMM,and an aggregated deviation of less than 8.7%for the micro dimple depth and 4%for the material removal amount between theory and experiment was obtained. | Minghuan WANG Yongchao SHANG Changshun LIU Jiajie WANG Jinsong ZHENG Xufeng XU | 2022 | Chinese Journal of Aeronautics2022，35，3： | 0 |
| 19 | Corrigendum to“Strontium modulates osteogenic activity of bone cement composed of bioactive borosilicate glass particles by activating Wnt/β-catenin signaling pathway”[Bioact.Mater.5(2020)334-347]显示文摘The authors regret that the printed version of the above article contained a number of errors which were not identified during the proofing stage.The correct and final version follows.The authors would like to apologies for any inconvenience caused.The authors regret:1.“…and the underlying molecular mechanism of this simulation is yet to be determined”,Page 335,needs to be corrected to“and the underlying molecular mechanism of this stimulation is yet to be determined”. | Xu Cui Yadong Zhang Jianyun Wang Chengcheng Huang Yudong Wang Hongsheng Yang Wenlong Liu Ting Wang Deping Wang Guocheng Wang Changshun Ruan Dafu Chen William W.Lu Wenhai Huang Mohamed N.Rahaman Haobo Pan | 2021 | Bioactive Materials2021，6，8： | 0 |
| 20 | Research on Transmission Line Tower Tilting and Foundation State Monitoring Technology Based onMulti-Sensor Cooperative Detection and Correction显示文摘The transmission line tower will be affected by bad weather and artificial subsidence caused by the foundation and other factors in the power transmission.The tower’s tilt and severe deformation will cause the building to collapse.Many small changes caused the tower’s collapse,but the early staff often could not intuitively notice the changes in the tower’s state.In the current tower online monitoring system,terminal equipment often needs to replace batteries frequently due to premature exhaustion of power.According to the need for real-time measurement of power line tower,this research designed a real-time monitoring device monitoring the transmission tower attitude tilting and foundation state based on the inertial sensor,the acceleration of 3 axis inertial sensor and angular velocity raw data to pole average filtering pre-processing,and then through the complementary filtering algorithm for comprehensive calculation of tilt angle,the system meets the demand for inclined online monitoring of power line poles and towers regarding measurement accuracy,with low cost and power consumption.The optimization multi-sensor cooperative detection and correction measured tilt angle result relative accuracy can reach 1.03%,which has specific promotion and application value since the system has the advantages of unattended and efficient calculation. | Guangxin Zhang Minghui Liu Shichao Cheng Minzhen Wang Changshun Zhao Hongdan Zhao Gaiming Zhong | 2024 | Energy Engineering2024，121，1： | 0 |
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