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| 1 | Interaction mechanisms of organic contaminants with burned straw ash charcoal显示文摘Black carbons(e.g.,charcoal) have a great impact on the transport of organic contaminants in soil and water because of its strong affnity and ubiquity in the environment.To further elucidate their interaction mechanism,sorption of polar(p-nitrotoluene,m-dinitrobenzene and nitrobenzene) and nonpolar(naphthalene) aromatic contaminants to burned straw ash charcoal under different de-ashed treatments were investigated.The sorption isotherms fitted well with Freundlich equation,and the Freundlich N values were all around 0.31-0.38,being independent of the sorbate properties and sorbent types.After sequential removal of ashes by acid treatments(HCl and HCl-HF) ,both adsorption and partition were enhanced due to the enrichment of charcoal component.The separated contribution of adsorption and partition to total sorption were quantified.The effective carbon content in ash charcoal functioned as adsorption sites,partition phases,and hybrid regions with adsorption and partition were conceptualized and calculated.The hybrid regions increased obviously after de-ashed treatment.The linear relationships of Freundlich N values with the charring-temperature of charcoal or biochar(the charred byproduct in biomass pyrolysis) were observed based on the current study and the cited publications which included 15 different temperatures(100-850°C) ,10 kinds of precursors of charcoal/biochar,and 10 organic sorbates. | Wenhai Huang,Baoliang Chen Department of Environmental Science,Zhejiang University,Hangzhou 310028,China | 2010 | Journal of Environmental Sciences2010，22，10： | 14 |
| 2 | Precise orbit determination of a maneuvered GEO satellite using CAPS ranging data显示文摘Wheel-off-loadings and orbital maneuvers of the GEO satellite result in additional accelerations to the satellite itself. Complex and difficult to model, these time varying accelerations are an important error source of precise orbit determination (POD). In most POD practices, only non-maneuver orbital arcs are treated. However, for some applications such as satellite navigation RDSS services, uninterrupted orbital ephemeris is demanded, requiring the development of POD strategies to be processed both during and after an orbital maneuver. We in this paper study the POD for a maneuvered GEO satellite, using high precision and high sampling rate ranging data obtained with Chinese Area Positioning System (CAPS). The strategy of long arc POD including maneuver arcs is studied by using telemetry data to model the maneuver thrust process. Combining the thrust and other orbital perturbations, a long arc of 6 days’ CAPS ranging data is analyzed. If the telemetry data are not available or contain significant errors, attempts are made to estimate thrusting parameters using CAPS ranging data in the POD as an alternative to properly account for the maneuver. Two strategies achieve reasonably good data fitting level in the tested arc with the maximal position difference being about 20 m. | HUANG Yong HU XiaoGong HUANG Cheng YANG QiangWen JIAO WenHai | 2009 | Science China(Physics,Mechanics & Astronomy)2009，52，3： | 13 |
| 3 | Preparation of bioactive glasses with controllable degradation behavior and their bioactive characterization显示文摘Bioactive glasses and ceramics have been widely investigated for bone repair because of their excel-lent bioactive characteristics. However, these biomaterials undergo incomplete conversion into a bone-like material, which severely limits their biomedical application. In this paper, borosilicate bioac-tive glasses were prepared by traditional melting process. The results showed that borosilicate glasses possessed high biocompatibility and bioactivity. In addition, when immersed in a 0.02 mol/L K2HPO4 solution, particles of a borate glass were fully converted to HA. The desirable conversion rate to HA may be achieved through the adjustment of the B2O3/SiO2 ratio. The results of XRD and FTIR analysis indicated that the degradation product was carbonate-substituted hydroxyapatite, which was similar to the inorganic component of bone. | YAO AiHua WANG DePing FU Qiang HUANG WenHai Mohamed N. RAHAMAN | 2007 | Chinese Science Bulletin2007，52，2： | 5 |
| 4 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 5 | Effect of background electrolytes on the adsorption of nitroaromatic compounds onto bentonite显示文摘To further elucidate interaction of nitroaromatic compounds with mineral surface, the sorption of m-dinitrobenzene (m-DNB) and nitrobenzene to original bentonite in aqueous solution containing different electrolytes (i.e., KCl, NH4Cl, CaCl2 and Tetramethy- lammonium bromide (TMAB)) was studied. The sorption of m-DNB was greatly enhanced with the presence of KCl and NH4Cl, while little influence was observed with CaCl2 and TMAB, following the order of KCl > NH4Cl TMAB, CaCl2, or DI water. For nitrobenzene, sorption enhancement only occurred at high nitrobenzene concentrations in the presence of KCl, and the solute equilibrium concentration at inflexion point was lowered with increasing KCl concentration. These sorption enhancements were significantly promoted with the increase of electrolyte concentration. The salting-out effect is insufficient to account for the sorption enhancement by original bentonite with increasing KCl or NH4Cl concentration. X-ray diffraction patterns of bentonite suspensions indicated that the sorption enhancement of m-DNB was attributed to the intercalation of K+ or NH4+ into bentonite interlayer and then dehydration with m-DNB to form inner-sphere complexes, which caused previously expanded bentonite interlayers to collapse in aqueous suspension, thus further enhanced the interaction of phenyl with siloxane surface. In comparison, the sorption enhancement of NB is attributed to the formation of outer-sphere complexes with K+ at high solute-loadings (> 200–400 mg/kg). The sorption of m-DNB to initially modified TMA+-bentonite and K+-bentonite was almost the same as respective sorption to original bentonite in solution containing TMA+ and K+. | CHEN Baoliang, HUANG Wenhai Department of Environmental Science, Zhejiang University, Hangzhou 310028, Zhejiang, China. | 2009 | Journal of Environmental Sciences2009，21，8： | 4 |
| 6 | Strontium modulates osteogenic activity of bone cement composed of bioactive borosilicate glass particles by activating Wnt/β-catenin signaling pathway显示文摘There is a need for synthetic grafts to reconstruct large bone defects using minimal invasive surgery.Our previous study showed that incorporation of Sr into bioactive borate glass cement enhanced the osteogenic capacity in vivo.However,the amount of Sr in the cement to provide an optimal combination of physicochemical properties and capacity to stimulate bone regeneration and the underlying molecular mechanism of this stimulation is yet to be determined.In this study,bone cements composed of bioactive borosilicate glass particles substituted with varying amounts of Sr(0 mol%to 12 mol%SrO)were created and evaluated in vitro and in vivo.The setting time of the cement increased with Sr substitution of the glass.Upon immersion in PBS,the cement degraded and converted more slowly to HA(hydroxyapatite)with increasing Sr substitution.The released Sr2+modulated the proliferation,differentiation,and mineralization of hBMSCs(human bone marrow mesenchymal stem cells)in vitro.Osteogenic characteristics were optimally enhanced with cement(designated BG6Sr)composed of particles substituted with 6mol%SrO.When implanted in rabbit femoral condyle defects,BG6Sr cement supported better peri-implant bone formation and bone-implant contact,comparing to cements substituted with 0mol%or 9mol%SrO.The underlying mechanism is involved in the activation of Wnt/β-catenin signaling pathway in osteogenic differentiation of hBMSCs.These results indicate that BG6Sr cement has a promising combination of physicochemical properties and biological performance for minimally invasive healing of bone defects. | Xu Cui Yadong Zhang Jianyun Wang Chengcheng Huang Yudong Wang Hongsheng Yang Wenlong Liu Ting Wang Deping Wang Guocheng Wang Changshun Ruan Dafu Chen William W.Lu Wenhai Huang Mohamed N.Rahaman Haobo Pan | 2020 | Bioactive Materials2020，5，2： | 3 |
| 7 | Development of a Zebrafish Model for Rapid Drug Screening against Alzheimer's Disease显示文摘 | Wenhai Huang Chuansheng Li Zhengrong Shen Xiaoyu Zhu Bo Xia Chunqi Li | 2016 | Journal of Pharmacy and Pharmacology2016，4，4： | 2 |
| 8 | Immunogenicity and protective efficacy of a recombinant protein subunit vaccine and an inactivated vaccine against SARS-CoV-2 variants in non-human primates显示文摘Emerging SARS-CoV-2 variants and the gradually decreasing neutralizing antibodies over time post vaccination have led to an increase in incidents of breakthrough infection across the world.To investigate the potential protective effect of the recombinant protein subunit COVID-19 vaccine targeting receptor-binding domain(RBD)(PS-RBD)and whole inactivated virus particle vaccine(IV)against the variant strains,in this study,rhesus macaques were immunized with PS-RBD or IV vaccine,followed by a Beta variant(B.1.351)challenge.Although neutralizing activity against the Beta variant was reduced compared with that against the prototype,the decreased viral load in both upper and lower respiratory tracts,milder pathological changes,and downregulated inflammatory cytokine levels in lung tissues after challenge demonstrated that PS-RBD and IV still provided effective protection against the Beta variant in the macaque model.Furthermore,PS-RBD-induced macaque sera possessed general binding and neutralizing activity to Alpha,Beta,Delta,and Omicron variants in our study,though the neutralizing antibody(NAb)titers declined by varying degrees,demonstrating potential protection of PS-RBD against current circulating variants of concern(VOCs).Interestingly,although the IV vaccine-induced extremely low neutralizing antibody titers against the Beta variant,it still showed reduction for viral load and significantly alleviated pathological change.Other correlates of vaccine-induced protection(CoP)like antibody-dependent cellular cytotoxicity(ADCC)and immune memory were both confirmed to be existing in IV vaccinated group and possibly be involved in the protective mechanism. | Qian He Qunying Mao Xiaozhong Peng Zhanlong He Shuaiyao Lu Jialu Zhang Fan Gao Lianlian Bian Chaoqiang An Wenhai Yu Fengmei Yang Yanan Zhou Yun Yang Yanyan Li Yadi Yuan Xujia Yan Jinghuan Yang Xing Wu Weijin Huang Changgui Li Junzhi Wang Zhenglun Liang Miao Xu | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 2 |
| 9 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 10 | Treatment of osteomyelitis and repair of bone defect by degradable bioactive borate glass releasing vancomycin显示文摘 | Zongping Xie Xin Liu Weitao Jia Changqing Zhang Wenhai Huang Jianqiang Wang | 2009 | Journal of Controlled Release2009，，2： | 1 |
| 11 | Prepa- ration and characterization of composite microspheres for brachytherapy and hyperthermia treatment of cancer 显示文摘 | Zhao Di Huang Wenhai Rahaman Mohamed | 2012 | Mater Sci Eng C2012，32，： | 1 |
| 12 | Strength of hollow hydroxyapatite microspheres prepared by a glass conversion process显示文摘 | Wenhai Huang Mohamed N. Rahaman Delbert E. Day Brad A. Miller | 2009 | Journal of Materials Science: Materials in Medicine2009，，1： | 1 |
| 13 | Fabrication of hydroxyapatite with highly ordered macroporous frame by colloidal templating method 显示文摘 | ZHOU Liyun WANG Deping HUANG Wenhai | 2009 | Mater Res Bull2009，44，2： | 1 |
| 14 | Enhanced sorption of naphthalene and nitroarnmane compounds to bentonite by potassium and cetyltrimethylamrnonium cations显示文摘 | CHEN Baoliang HUANG Wenhai MAO Jiefei | 2008 | Journal of Hazardous Materials2008，158，1： | 1 |
| 15 | 显示文摘 | Lin Jian Huang Wenhai Lei Shuhua | 2008 | Journal of Ceramic Processing Research2008，4，： | 1 |
| 16 | Complex primary crystallization kinetics of amorphous finemet alloy显示文摘 | Lu Wei Yan Biao Huang Wenhai | 2005 | J Noncryst Solids2005，351，： | 1 |
| 17 | Bioactive borate glass scaffolds: in vitro and in vivo evaluation for use as a drug delivery system in the treatment of bone infection显示文摘 | Xin Liu Zongping Xie Changqing Zhang Haobo Pan Mohamed N. Rahaman Xin Zhang Qiang Fu Wenhai Huang | 2010 | Journal of Materials Science: Materials in Medicine2010，，2： | 1 |
| 18 | Complex primary crystallization kinetics of amorphous finement alloy显示文摘 | Lu Wei Yan Biao Huang Wenhai | 2005 | J of Non-crystalline Solids2005，351，4042： | 1 |
| 19 | Evaluation of the proliferation and differentiation behaviors of mesenchymalstem cells with partially converted borate glass containing different amountsof strontium in vitro显示文摘 | Yi Zhu Yuanming Ouyang Yi Chang Congfeng Luo Jun Xu Changqing Zhang Wenhai Huang | 2013 | Molecular Medicine Reports2013，，4： | 1 |
| 20 | In Vitro Comparative Effect of Three Novel Borate Bioglasses on the Behaviors of Osteoblastic MC3T3-E1 Cells显示文摘Most related investigations focused on the effects of borate glass on cell proliferation/biocompatibility in vitro or bone repair in vivo;however,very few researches were carried out on other cell behaviors.Three novel borate bioglasses were designed as scaffolds for bone regeneration in this wok.Comparative effects of three bioglasses on the behaviors of osteoblastic MC3T3-E1 cells were evaluated.Excellent cytocompatibility of these novel borate bioglasses were approved in this work.Meanwhile,the promotion on cell proliferation,protein secretion and migration with minor cell apoptosis were also discussed in details,which contributed to the potential clinical application as a new biomaterial for orthopedics. | Xiaojuan Wei Tingfei Xi Yufeng Zheng Changqing Zhang Wenhai Huang | 2014 | Journal of Materials Science & Technology2014，30，10： | 1 |
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