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| 1 | Haplotype-resolved genome of diploid ginger (Zingiber officinale) and its unique gingerol biosynthetic pathway显示文摘Ginger(Zingiber officinale),the type species of Zingiberaceae,is one of the most widespread medicinal plants and spices.Here,we report a high-quality,chromosome-scale reference genome of ginger‘Zhugen’,a traditionally cultivated ginger in Southwest China used as a fresh vegetable,assembled from PacBio long reads,Illumina short reads,and high-throughput chromosome conformation capture(Hi-C)reads.The ginger genome was phased into two haplotypes,haplotype 1(1.53 Gb with a contig N50 of 4.68 M)and haplotype 0(1.51 Gb with a contig N50 of 5.28 M).Homologous ginger chromosomes maintained excellent gene pair collinearity.In 17,226 pairs of allelic genes,11.9%exhibited differential expression between alleles.Based on the results of ginger genome sequencing,transcriptome analysis,and metabolomic analysis,we proposed a backbone biosynthetic pathway of gingerol analogs,which consists of 12 enzymatic gene families,PAL,C4H,4CL,CST,C3’H,C3OMT,CCOMT,CSE,PKS,AOR,DHN,and DHT.These analyses also identified the likely transcription factor networks that regulate the synthesis of gingerol analogs.Overall,this study serves as an excellent resource for further research on ginger biology and breeding,lays a foundation for a better understanding of ginger evolution,and presents an intact biosynthetic pathway for species-specific gingerol biosynthesis. | Hong-Lei Li Lin Wu Zhaoming Dong Yusong Jiang Sanjie Jiang Haitao Xing Qiang Li Guocheng Liu Shuming Tian Zhangyan Wu Bin Wu Zhexin Li Ping Zhao Yan Zhang Jianmin Tang Jiabao Xu Ke Huang Xia Liu Wenlin Zhang Qinhong Liao Yun Ren Xinzheng Huang Qingzhi Li Chengyong Li Yi Wang Baskaran Xavier-Ravi Honghai Li Yang Liu Tao Wan Qinhu Liu Yong Zou Jianbo Jian Qingyou Xia Yiqing Liu | 2021 | Horticulture Research2021，8，1： | 2 |
| 2 | Incidence,genomic diversity,and evolution of strawberry mottle virus in China显示文摘Strawberry mottle virus(SMoV)is one of the most common viruses infecting strawberries,causing losses to fruit yield and quality.In this study,165 strawberry leaf samples were collected from six provinces of China,46 of which tested positive for SMoV.The complete genome sequences of 11 SMoV isolates were obtained from Liaoning(DGHY3,DGHY16-2,DGHY17,DGHY20-2,DGHY21,DGHY26-2),Shandong(SDHY1,SDHY5,SDHY31-2,SDHY33-2),and Beijing(BJMX7).The RNA1 and RNA2 nucleotide identities between the 11 Chinese isolates were 95.4-99.3%and 96.3-99.6%,respectively,and they shared 78.4-96.6%and 84.8-93.5%identities with the available SMoV isolates in GenBank.Recombination analysis revealed that Chinese isolate SDHY33-2 and Canadian isolates Ontario and Simcoe were recombinants,and recombination events frequently occurred in the 3’UTR of SMoV.Phylogenetic analysis showed that in an RNA1 tree,most Chinese isolates clustered into the same group while isolate DGHY17 clustered into another group together with Czech isolate C and three Canadian isolates.In an RNA2 tree,all Chinese isolates clustered into a single group.The phylogenetic analysis based on nucleotide sequences was consistent with the results based on coat protein(CP)and RNA-dependent RNA polymerase(RdRp).Further evolutionary analysis indicated that negative selection drives SMoV evolution,and gene flow plays a major role in genetic differentiation.Additionally,reassortment and recombination also influence the evolution of SMoV.To our knowledge,this is the first report of the complete genome of SMoV isolates from China and a detailed analysis of the SMoV population structure. | LINGJIAO FAN CHENGYONG HE MENGMENG WU DEHANG GAO ZHENFEI DONG SHENGFAN HOU ZEKUN FENG HONGQING WANG | 2021 | BIOCELL2021，45，4： | 1 |
| 3 | Correction:Haplotype-resolved genome of diploid ginger (Zingiber officinale)and its unique gingerol biosynthetic pathway显示文摘After online publication of the article 1,the authors noticed the affiliation“College of Landscape Architecture and Life Science/Institute of Special Plants,Chongqing University of Arts and Sciences,Yongchuan,Chongqing,China”for author Yiqing Liu was missing. | Hong-Lei Li Lin Wu Zhaoming Dong Yusong Jiang Sanjie Jiang Haitao Xing Qiang Li Guocheng Liu Shuming Tian Zhangyan Wu Bin Wu Zhexin Li Ping Zhao Yan Zhang Jianmin Tang Jiabao Xu Ke Huang Xia Liu Wenlin Zhang Qinhong Liao Yun Ren Xinzheng Huang Qingzhi Li Chengyong Li Yi Wang Baskaran Xavier-Ravi Honghai Li Yang Liu Tao Wan Qinhu Liu Yong Zou Jianbo Jian Qingyou Xia Yiqing Liu | 2021 | Horticulture Research2021，8，1： | 1 |
| 4 | A unique two-photon fluorescent probe based on ICT mechanism for imaging palladium in living cells and mice显示文摘Palladium(0)as one of the vital transition metals,is employed in numerous industries,such as drug synthesis,aerospace high-tech field and automobile industry.When the Pd(0)enter into the body,it will bind with thiol-containing amino acids,DNA,RNA,and other biomolecules damaging to human health.Thus,developing a novel tool for monitoring and imaging of Pd(0)in vivo is very urgent.In the work,based on a intramolecular charge transfer(ICT)mechanism a two-photon fluorescent probe NIPd had been designed and synthesized for the recognition Pd(0).In vitro experiments data displayed that probe NIPd exhibited a 13-fold fluorescent increase for Pd(0)in 30 min in the aqueous solution with a detection limit of 16 nmol/L.It also showed the outstanding selectivity and antijamming performance.More importantly,NIPd could be served as a two-photon fluorescent probe for real-time monitoring Pd(0)in living cells and mice. | Shuangzhe Zhang Haidong Li Qjchao Yao Sahar Ghazali Jiangli Fan Jianjun Du Jingyun Wang Fengli Gao Miao Li Haibo Wang Chengyong Dong Xiaojun Peng | 2020 | Chinese Chemical Letters2020，31，11： | 1 |
| 5 | Recent Advances in Processing of Biological Tissues显示文摘The advancement of surgical technology has greatly improved the capability of clinical treatments in recent decades.Advanced surgical technologies,such as minimally invasive,implantation interventional,and robotassisted surgeries,have been emerging,and bring enormous benefits to patients.While these technologies have been widely employed in clinical applications,the fundamentals of the clinical surgeries can also be interpreted in an engineering term as the processing and manipulation of biological tissues and has been merging with biomechanics,materials science and manufacturing science,although surgical technology may stand and often treated as an individual discipline. | Chengyong Wang Zhirong Liao Dong Wang | 2022 | Chinese Journal of Mechanical Engineering2022，35，3： | 0 |
| 6 | Recent Advances in Soft Biological Tissue Manipulating Technologies显示文摘Biological soft tissues manipulation,including conventional(mechanical)and nonconventional(laser,waterjet and ultrasonic)processes,is critically required in most surgical innervations.However,the soft tissues,with their nature of anisotropic and viscoelastic mechanical properties,and high biological and heat sensitivities,are difficult to manipulated.Moreover,the mechanical and thermal induced damage on the surface and surrounding tissue during the surgery can impair the proliferative phase of healing.Thus,understanding the manipulation mechanism and the resulted surface damage is of importance to the community.In recent years,more and more scholars carried out researches on soft biological tissue cutting in order to improve the cutting performance of surgical instruments and reduce the surgery induced tissue damage.However,there is a lack of compressive review that focused on the recent advances in soft biological tissue manipulating technologies.Hence,this review paper attempts to provide an informative literature survey of the state-of-the-art of soft tissue manipulation processes in surgery.This is achieved by exploring and recollecting the different soft tissue manipulation techniques currently used,including mechanical,laser,waterjet and ultrasonic cutting and advanced anastomosis and reconstruction processes,with highlighting their governing removal mechanisms as well as the surface and subsurface damages. | Zhihua Liu Zhirong Liao Dong Wang Chengyong Wang Chengli Song Haonan Li Yao Liu | 2022 | Chinese Journal of Mechanical Engineering2022，35，3： | 0 |
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