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| 1 | Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice显示文摘Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity. | Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang | 2021 | Molecular Plant2021，14，2： | 8 |
| 2 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 3 | Construction of Non-infectious SARS-CoV-2 Replicons and Their Application in Drug Evaluation显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused a devastating pandemic worldwide.Vaccines and antiviral drugs are the most promising candidates for combating this global epidemic,and scientists all over the world have made great efforts to this end.However,manipulation of the SARS-CoV-2 should be performed in the biosafety level3 laboratory.This makes experiments complicated and time-consuming.Therefore,a safer system for working with this virus is urgently needed.Here,we report the construction of plasmid-based,non-infectious SARS-CoV-2 replicons with turbo-green fluorescent protein and/or firefly luciferase reporters by reverse genetics using transformation-associated recombination cloning in Saccharomyces cerevisiae.Replication of these replicons was achieved simply by direct transfection of cells with the replicon plasmids as evident by the expression of reporter genes.Using SARS-CoV-2 replicons,the inhibitory effects of E64-D and remdesivir on SARS-CoV-2 replication were confirmed,and the halfmaximal effective concentration(EC50)value of remdesivir and E64-D was estimated by different quantification methods respectively,indicating that these SARS-CoV-2 replicons are useful tools for antiviral drug evaluation. | Bei Wang Chongyang Zhang Xiaobo Lei Lili Ren He Huang Jianwei Wang Zhendong Zhao | 2021 | Virologica Sinica2021，36，5： | 3 |
| 4 | Anaerobic phenanthrene biodegradation with four kinds of electron acceptors enriched from the same mixed inoculum and exploration of metabolic pathways显示文摘Polycyclic aromatic hydrocarbons (PAHs) are widespread and persistent contaminants worldwide, especially in environments devoid of molecular oxygen. For lack of molecular oxygen, researchers enhanced anaerobic zones PAHs biodegradation by adding sulfate, bicarbonate, nitrate, and iron. However, microbial community reports of them were limited, and information of metabolites was poor except two-ring PAH, naphthalene. Here, we reported on four phenanthrene-degrading enrichment cultures with sulfate, bicarbonate, nitrate, and iron as electron acceptors from the same initial inoculum. The high-to-low order of the anaerobic phenanthrene biodegradation rate was the nitrate-reducing conditions>sulfate-reducing conditions>methanogenic conditions>iron-reducing conditions. The dominant bacteria populations were Desulfobacteraceae, Anaerolinaceae, and Thermodesulfobiaceae under sulfate-reducing conditions;Moraxellaceae, Clostridiaceae, and Comamonadaceae under methanogenic conditions;Rhodobacteraceae, Planococcaceae, and Xanthomonadaceae under nitrate-reducing conditions;and Geobacteraceae, Carnobacteriaceae, and Anaerolinaceae under iron-reducing conditions, respectively. Principal component analysis (PCA) indicated that bacteria populations of longtime enriched cultures with four electron acceptors all obtained significant changes from original inoculum, and bacterial communities were similar under nitrate-reducing and iron-reducing conditions. Archaea accounted for a high percentage under iron-reducing and methanogenic conditions, and Methanosarcinaceae and Methanobacteriaceae, as well as Methanobacteriaceae, were the dominant archaea populations under iron-reducing and methanogenic conditions. The key steps of phenanthrene biodegradation under four reducing conditions were carboxylation, further ring system reduction, and ring cleavage. | Zuotao Zhang Chongyang Wang Jianzhong He Hui Wang | 2019 | Frontiers of Environmental Science & Engineering2019，13，5： | 3 |
| 5 | Simultaneous acquisition of multi-nuclei enhanced NMR/MRI by solution-state dynamic nuclear polarization显示文摘Dynamic nuclear polarization(DNP) has become a very important hyperpolarization method because it can dramatically increase the sensitivity of nuclear magnetic resonance(NMR) of various molecules.Liquid-state DNP based on Overhauser effect is capable of directly enhancing polarization of all kinds of nuclei in the system.The combination of simultaneous Overhauser multi-nuclei enhancements with the multi-nuclei parallel acquisitions provides a variety of important applications in both MR spectroscopy(MRS) and image(MRI).Here we present two simple illustrative examples for simultaneously enhanced multi-nuclear spectra and images to demonstrate the principle and superiority.We have observed very large simultaneous DNP enhancements for different nuclei,such as ~1H and ^(23)Na,~1H and ^(31)P,^(19)F and ^(31)P,especially for the first time to report sodium ion enhancement in liquid.We have also obtained the simultaneous images of ^(19)H and ^(31)P,^(19)F and ^(31)P at low field by solution-state DNP for the first time. | Yugui He Zhen Zhang Jiwen Feng Chongyang Huang Fang Chen Chaoyang Liu Maili Liu | 2016 | Science China Chemistry2016，59，7： | 2 |
| 6 | In-situ structural evolution of Bi_(2)O_(3) nanoparticle catalysts for CO_(2) electroreduction显示文摘Under the complex external reaction conditions,uncovering the true structural evolution of the catalyst is of profound significance for the establishment of relevant structure–activity relationships and the rational design of electrocatalysts.Here,the surface reconstruction of the catalyst was characterized by ex-situ methods and in-situ Raman spectroscopy in CO_(2)electroreduction.The final results showed that the Bi_(2)O_(3) nanoparticles were transformed into Bi/Bi_(2)O_(3) two-dimensional thin-layer nanosheets(NSs).It is considered to be the active phase in the electrocatalytic process.The Bi/Bi_(2)O_(3) NSs showed good catalytic performance with a Faraday efficiency(FE)of 94.8%for formate and a current density of 26 mA cm^(−2) at−1.01 V.While the catalyst maintained a 90%FE in a wide potential range(−0.91 V to−1.21 V)and long-term stability(24 h).Theoretical calculations support the theory that the excellent performance originates from the enhanced bonding state of surface Bi-Bi,which stabilized the adsorption of the key intermediate OCHO^(∗) and thus promoted the production of formate. | Hongbo Wang Chongyang Tang Bo Sun Jiangchao Liu Yan Xia Wenqing Li Changzhong Jiang Dong He Xiangheng Xiao | 2022 | International Journal of Extreme Manufacturing2022，4，3： | 1 |
| 7 | Function of hydroxycinnamoyl transferases for the biosynthesis of phenolamides in rice resistance to Magnaporthe oryzae显示文摘Phenolamide(PA)metabolites play important roles in the interaction between plants and pathogens.The putrescine hydroxycinnamoyl transferase genes Os PHT3 and Os PHT4 positively regulate rice cell death and resistance to Magnaporthe oryzae.The b ZIP transcription factor APIP5,a negative regulator of cell death and rice immunity,directly binds to the Os PHT4 promoter to regulate putrescine-derived PAs.Whether other hydroxycinnamoyl transferase(HT)genes also participate in APIP5-mediated immunity remains unclear.Surprisingly,we find that genes encoding agmatine hydroxycinnamoyl transferases Os AHT1 and Os AHT2,tryptamine hydroxycinnamoyl transferases Os TBT1 and Os TBT2,and tyramine hydroxycinnamoyl transferases Os THT1 and Os THT2,responsible for the biosynthesis of polyamine-derived PAs are all up-regulated in APIP5-RNAi transgenic plants compared with segregated wild-type rice.Furthermore,both Os AHT1/2 and Os TBT1/2 are induced during M.oryzae infection,showing expression patterns similar to those previously reported for Os THT1/2 and Os PHT3/4.Transgenic plants overexpressing either Os AHT2-GFP or Os TBT1-GFP show enhanced resistance against M.oryzae and accumulated more PA metabolites and lignin compared with wild-type plants.Interestingly,as demonstrated for Os PHT4,APIP5 directly binds to the promoters of Os AHT1/2,Os TBT1/2,and Os THT1/2,repressing their transcription.Together,these results indicate that the HT genes are common targets of APIP5 and that PAs play critical roles in rice immunity. | Hong Fang Fan Zhang Chongyang Zhang Dan Wang Shuangqian Shen Feng He Hui Tao Ruyi Wang Min Wang Debao Wang Xionglun Liu Jie Luo Guo-Liang Wang Yuese Ning | 2022 | Journal of Genetics and Genomics2022，49，8： | 1 |
| 8 | The d-orbital coupling modulation of CuNi alloy for acetonitrile electrochemical reduction and in-situ hydrogenation behavior characterization显示文摘Electrochemical reduction of acetonitrile to ethylamine with a high selectivity is a novel approach to manufacture valuable primary amines which are important raw material in organic chemical industry. However, the poor ethylamine Faradic efficiency(FE_(ethylamine)) and catalyst stability at the high current density prohibit this method from being practically used. Herein, CuNi alloy ultrafine-nano-particles based on the d-orbital coupling modulation were synthesized through the electrodeposition and their catalytic performance towards acetonitrile reduction reaction(ACNRR) has been systematically studied. The highest FE_(ethylamine)(97%) is achieved with the current density of-114 mA cm^(-2). For practical application, the current density can reach-602.8 mA cm^(-2) with 82.8% FE_(ethylamine)maintained. With the appearance of other organics which co-exist with acetonitrile in the SOHIO process, CuNi can also hydrogenate acetonitrile in it with more than 80% FE_(ethylamine). Our in-situ spectroscopy analysis and DFT calculations towards the acetonitrile hydrogenation behavior reveal that the evenly dispersed Ni in Cu modulates the dband so as to endow CuNi with the better acetonitrile adsorption, milder binding energy with the reaction intermediates, smaller barrier for *CH_3CH_2NH_2 desorption and higher ability for H_2O dissociation to provide *H. | Boling Liu Dong He Zunjian Ke Hongbo Wang Chongyang Tang Qi Zhang Hang Xu Menghua Yang Yafei Yang Qi Liu Xiangheng Xiao | 2023 | Science China Chemistry2023，66，11： | 0 |
| 9 | Fluid-Dynamics Analysis and Structural Optimization of a 300 kW MicroGas Turbine Recuperator显示文摘Computational Fluid Dynamics(CFD)is used here to reduce pressure loss and improve heat exchange efficiency in the recuperator associated with a gas turbine.First,numerical simulations of the high-temperature and lowtemperature channels are performed and,the calculated results are compared with experimental data(to verify the reliability of the numerical method).Second,the flow field structure of the low-temperature side channel is critically analyzed,leading to the conclusion that the flow velocity distribution in the low-temperature side channel is uneven,and its resistance is significantly higher than that in the high-temperature side.Therefore,five alternate structural schemes are proposed for the optimization of the low-temperature side.In particular,to reduce the flow velocity in the upper channel,the rib length of each channel at the inlet of the low-temperature side region is adjusted.The performances of the 5 schemes are compared,leading to the identification of the configuration able to guarantee a uniform flow rate and minimize the pressure drop.Finally,the heat transfer performance of the optimized recuperator structure is evaluated,and it is shown that the effectiveness of the recuperator is increased by 1.5%. | Weiting Jiang Tingni He Chongyang Wang Weiguo Pan Jiang Liu | 2023 | Fluid Dynamics & Materials Processing2023，19，6： | 0 |
| 10 | Electronic Coupling of Single Atom and FePS_3 Boosts Water Electrolysis显示文摘Engineering the electronic structure of surface active sites at the atomic level can be an efficient way to modulate the reactivity of catalysts.Herein,we report the rational tuning of surface electronic structure of FePS_(3) nanosheets(NSs)by anchoring atomically dispersed metal atom.Theoretical calculations predict that the strong electronic coupling effect in single-atom Ni-FePS_(3) facilitates electron aggregation from Fe atom to the nearby Ni-S bond and enhances the electron-transfer of Ni and S sites,which balances the oxygen species adsorption capacity,reinforces water adsorption and dissociation process to accelerate corresponding oxygen evolution reaction(OER)and hydrogen evolution reaction(HER).The optimal Ni-FePS_(3)NSs/C exhibits outstanding electrochemical water-splitting activities,delivering an overpotential of 287 mV at the current density of 10 mA cm^(-2) and a Tafel slope of 41.1 mV dec^(-1) for OER;as well as an overpotential decrease of 219 mV for HER compared with pure FePS_(3)NSs/C.The concept of electronic coupling interaction between the substrate and implanted single active species offers an additional method for catalyst design and beyond. | Chongyang Tang Dong He Nan Zhang Xianyin Song Shuangfeng Jia Zunjian Ke Jiangchao Liu Jianbo Wang Changzhong Jiang Ziyu Wang Xiaoqing Huang Xiangheng Xiao | 2022 | Energy & Environmental Materials2022，5，3： | 0 |
| 11 | 水稻通过抑制稻瘟菌效应蛋白引起的细胞坏死抵御稻瘟菌的侵染显示文摘由稻瘟菌引起的稻瘟病是水稻生产中最严重的真菌性病害。然而,稻瘟菌如何引起水稻细胞坏死,以及水稻如何抑制稻瘟菌从活体营养阶段转换到死体营养阶段的分子机制还不清楚。 | Ruyi Wang Yuese Ning Xuetao Shi Feng He Chongyang Zhang Jiangbo Fan Nan Jiang Yu Zhang Ting Zhang Yajun Hu Maria Bellizzi 王国梁 | 2017 | 科学新闻2017，19，4： | 0 |
| 12 | Effects of vibration conditions,spatial confinement and friction on mixing and segregation characteristics of mixed pebble beds for CFETR WCCB blanket显示文摘This paper presents the study of the dynamic characteristics of confined mixed pebble beds with different friction under different vibration conditions using the discrete element method.Theλsegre-gation index is used to quantify the degree of particle mixing or segregation.The percolation,convection and diffusion mechanisms are responsible for the segregation patterns of the mixed pebble.The results show the degree of segregation can be suppressed by decreasing the vibration acceleration or free space height below a threshold.Further simulation reveals the threshold of vibration acceleration or free space height both are related to the bed height increment which determines the strength of the percolation mechanism.In addition,the strength of percolation and convection becomes weaker by decreasing the friction of particles and walls,which makes the pebbled bed remains in a mixed state under vibration.These findings are significant to clarify the main factors behind the three segregation mechanisms and hence provide solutions to retaining the mixed state of the Li2TiO3&Be12Ti mixed pebble bed. | Yong Liu Chongyang He Cong Wang Lei Chen Songlin Liu | 2024 | Particuology2024，85，2： | 0 |
| 13 | Artificial Intelligence Based Multi-Scenario mmWave Channel Modeling for Intelligent High-Speed Train Communications显示文摘A large amount of mobile data from growing high-speed train(HST)users makes intelligent HST communications enter the era of big data.The corresponding artificial intelligence(AI)based HST channel modeling becomes a trend.This paper provides AI based channel characteristic prediction and scenario classification model for millimeter wave(mmWave)HST communications.Firstly,the ray tracing method verified by measurement data is applied to reconstruct four representative HST scenarios.By setting the positions of transmitter(Tx),receiver(Rx),and other parameters,the multi-scenarios wireless channel big data is acquired.Then,based on the obtained channel database,radial basis function neural network(RBF-NN)and back propagation neural network(BP-NN)are trained for channel characteristic prediction and scenario classification.Finally,the channel characteristic prediction and scenario classification capabilities of the network are evaluated by calculating the root mean square error(RMSE).The results show that RBF-NN can generally achieve better performance than BP-NN,and is more applicable to prediction of HST scenarios. | Zhang Mengjiao Liu Yu Huang Jie He Ruisi Zhang Jingfan Yu Chongyang Wang Chengxiang | 2024 | China Communications2024，21，3： | 0 |
| 14 | Simulation and Optimization of the Fluid Solidification Process in Brazed Plate Heat Exchangers显示文摘When a brazed plate heat exchanger is used as an evaporator,the working mass in the channel may undergo soli-dification,thereby hindering the refrigeration cycle.In this study the liquid solidification process and its optimi-zation in a brazed plate heat exchanger are investigated numerically for different inlet velocities;moreover,different levels of corrugation are considered.The results indicate that solidificationfirst occurs around the con-tacts,followed by the area behind the contacts.It is also shown that deadflow zones exist in the sharp areas and such areas are prone to liquid solidification.After optimization,the solidification area attains its smallest value when a corrugation spacingλ=4.2 mm is considered. | Weiting Jiang Lei Zhao Chongyang Wang Tingni He Weiguo Pan | 2023 | Fluid Dynamics & Materials Processing2023，19，10： | 0 |
| 15 | Recombinant human erythropoietin ameliorates cognitive dysfunction of APP/PS1 mice by attenuating neuron apoptosis via HSP90β显示文摘Dear Editor,Recently,lower hemoglobin/anemia in the elderly is found to be associated with cognitive impairment and Alzheimer’s disease(AD),1 implying that erythropoietin(EPO)may be of benefit for AD.As a clinically safe-to-use drug,besides its hematopoietic function,recombinant human EPO(rhEPO)is reported to present multifaceted neuroprotective effects.2 Unfortunately,few clinical studies investigated the effect of rhEPO in AD patients. | Hua-Li Wan Bing-Ge Zhang Chongyang Chen Qian Liu Ting Li Ye He Yongmei Xie Xifei Yang Jian-Zhi Wang Gong-Ping Liu | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 0 |
| 16 | Highly elastic energy storage device based on intrinsically super-stretchable polymer lithium-ion conductor with high conductivity显示文摘Stretchable power sources,especially stretchable lithium-ion batteries(LIBs),have attracted increasing attention due to their enormous prospects for powering flexible/wearable electronics.Despite recent advances,it is still challenging to develop ultra-stretchable LIBs that can withstand large deformation.In particular,stretchable LIBs require an elastic electrolyte as a basic component,while the conductivity of most elastic electrolytes drops sharply during deformation,especially during large deformations.This is why highly stretchable LIBs have not yet been realized until now.As a proof of concept,a super-stretchable LIB with strain up to 1200%is created based on an intrinsically super-stretchable polymer electrolyte as the lithium-ion conductor.The super-stretchable conductive system is constructed by an effective diblock copolymerization strategy via photocuring of vinyl functionalized 2-ureido-4-pyrimidone(VFUpy),an acrylic monomer containing succinonitrile and a lithium salt,achieving high ionic conductivity(3.5×10^(-4)mS cm^(-1)at room temperature(RT))and large deformation(the strain can reach 4560%).The acrylic elastomer containing Li-ion conductive domains can strongly increase the compatibility between the neighboring elastic networks,resulting in high ionic conductivity under ultra-large deformation,while VFUpy increases elasticity modulus(over three times)and electrochemical stability(voltage window reaches 5.3 V)of the prepared polymer conductor.At a strain of up to 1200%,the resulting stretchable LIBs are still sufficient to power LEDs.This study sheds light on the design and development of high-performance intrinsically super-stretchable materials for the advancement of highly elastic energy storage devices for powering flexible/wearable electronics that can endure large deformation. | Shi Wang Jixin He Qiange Li Yu Wang Chongyang Liu Tao Cheng Wen-Yong Lai | 2024 | Fundamental Research2024，4，1： | 0 |
| 17 | Transcriptome Analysis of oserf922 Mutants Reveals New Insights into Rice Blast Resistance显示文摘Rice blast disease,caused by fungal Magnaporthe oryzae,severely threatens food security.The susceptibility(S) genes have emerged to support pathogenesis,and disruption of S gene usually confers increased resistance to multiple pathogen isolates.Rice ethylene response factor gene Os ERF922 is a potential S gene in blast disease.However,how Os ERF922 negatively regulates resistance against M.oryzae is still unknown. | QIN Mengchao TAO Hui SHI Xuetao ZHANG Chongyang HE Feng WANG Min LIU Zheng WANG Jisong ZHANG Rongxue WANG Shutong WANG Guoliang NING Yuese WANG Ruyi | 2023 | Rice science2023，30，5： | 0 |
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