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| 1 | Articular cartilage and osteochondral tissue engineering techniques:Recent advances and challenges显示文摘In spite of the considerable achievements in the field of regenerative medicine in the past several decades,osteochondral defect regeneration remains a challenging issue among diseases in the musculoskeletal system because of the spatial complexity of osteochondral units in composition,structure and functions.In order to repair the hierarchical tissue involving different layers of articular cartilage,cartilage-bone interface and subchondral bone,traditional clinical treatments including palliative and reparative methods have showed certain improvement in pain relief and defect filling.It is the development of tissue engineering that has provided more promising results in regenerating neo-tissues with comparable compositional,structural and functional characteristics to the native osteochondral tissues.Here in this review,some basic knowledge of the osteochondral units including the anatomical structure and composition,the defect classification and clinical treatments will be first introduced.Then we will highlight the recent progress in osteochondral tissue engineering from perspectives of scaffold design,cell encapsulation and signaling factor incorporation including bioreactor application.Clinical products for osteochondral defect repair will be analyzed and summarized later.Moreover,we will discuss the current obstacles and future directions to regenerate the damaged osteochondral tissues. | Wenying Wei Honglian Dai | 2021 | Bioactive Materials2021，6，12： | 14 |
| 2 | Preparation and characterization of a degradable magnesium phosphate bone cement显示文摘A kind of degradable magnesium phosphate bone cement(MPBC)was fabricated by using the mixed powders of magnesium oxide(MgO),potassium dihydrogen phosphate(KH_(2)PO_(4))and calcium dihydrogen phosphate(Ca(H_(2)PO_(4))2.H_(2)O).As MgKPO_(4),the main product of MgO and KH_(2)PO_(4)was alkaline,the Ca(H_(2)PO_(4))2.H_(2)Owas added to neutralize the alkali of the system.And the effects of Ca(H_(2)PO_(4))2.H_(2)Oon the performance of MPBC were discussed.The results showed that the adding of Ca(H_(2)PO_(4))2.H_(2)Oextended the setting time,which was about 6 min to 18 min.The compressive strength increased first and then decreased,and maximum value reached 31.2MPa after setting for 24 h without any additional pressure.The MPBC was degradable in Tris–HCl solution,and the extracts of the cytotoxicity assay showed that the MPBC had good biocompatibility,indicating that the MPBC had good biodegradable and biocompatible properties. | Ying Yu Chao Xu Honglian Dai | 2016 | Regenerative Biomaterials2016，3，4： | 6 |
| 3 | Iron oxide nanoparticles with photothermal performance and enhanced nanozyme activity for bacteria-infected wound therapy显示文摘Metal-based nanomaterials usually have broad-spectrum antibacterial properties,low biological toxicity and no drug resistance due to their intrinsic enzyme-like catalytic properties and external field(magnetic,thermal,acoustic,optical and electrical)responsiveness.Herein,iron oxide(Fe_(3)O_(4))nanoparticles(IONPs)synthesized by us have good biosafety,excellent photothermal conversion ability and peroxidase-like catalytic activity,which can be used to construct a photothermal-enzymes combined antibacterial treatment platform.IONPs with peroxide-like catalytic activity can induce H_(2)O_(2)to catalyze the production of·OH in a slightly acidic environment,thus achieving certain bactericidal effects and increasing the sensitivity of bacteria to heat.When stimulated by near-infrared light,the photothermal effect could destroy bacterial cell membranes,resulting in cleavage and inactivation of bacterial protein,DNA or RNA.Meanwhile,it can also improve the catalytic activity of peroxidase-like and promote IONPs to catalyze the production of more·OH for killing bacteria.After IONPs synergistic treatment,the antibacterial rate of Escherichia coli and Staphylococcus aureus reached nearly 100%.It also has an obvious killing effect on bacteria in infected wounds of mice and can effectively promote the healing of S.aureusinfected wounds,which has great application potential in clinical anti-infection treatment. | Jiaxin Guo Wenying Wei Yanan Zhao Honglian Dai | 2022 | Regenerative Biomaterials2022，9，1： | 3 |
| 4 | Effect of Hydroxyapatitc Nanoparticles on K562 Cells in vitro显示文摘稳定、分散单人赛的 hydroxyapatite (幸运) nanoparticles 与超声帮助方法被综合。幸运 nanoparticles 被动态轻散布, XRD (X 光检查衍射) 和 TEM (传播电子显微镜学) 描绘。K562 人的骨髓内产生的白血病房间线上的幸运 nanoparticles 的效果被 MTT 试金和房间计数测试调查,并且机制通过房间周期和超微结构的变化被学习。结果证明幸运 nanoparticles 在 vitro 戏剧性地禁止了 K562 房间的增长。幸运 nanoparticles 进入了 K562 房间的细胞质,这样,房间在 G2/M 阶段被逮捕房间直接死了。 | CHEN Pei DAI Honglian HAN Yingchao YIN Meizhen LI Shipu | 2008 | Journal of Wuhan University of Technology(Materials Science)2008，23，2： | 2 |
| 5 | Preparation of Nano-TiO_2 by Liquid Hydrolysis and Characterization of Its Antibacterial Activity显示文摘The nano-TiO2 particles were prepared by liquid hydrolysis method and characterized using XRD. Its antibacterial activity against two representative bacterial, Escherichia coli and Staphylococcus aureus, was also studied. The experimental results showed that the nano-TiO2 calcinated at 600-700 ℃ contained the obvious anatase phase and exerted excellent antibacterial activity. The feature of antibacterial activity of nanoTiO2 was non-strains specificity and exerted best antibacterial activity at concentration of 0.8 g/L. | 张启焕 YAN Xin 邵荣 DAI Honglian LI Shipu | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，2： | 2 |
| 6 | A New Type of Nanogel Carrier based on Mixed Pluronic Loaded with Low-Dose Antitumor Drugs显示文摘To design a new type of antitumor nanodrug carrier with good biocompatibility, a drug delivery system with a 2.19% drug-loading rate, measured by high-performance liquid chromatography(HPLC), was prepared by membrane hydration using a mixed polymer: Pluronic■ F-127, which binds folic acid(FA), Pluronic■ F-68 and triptolide(TPL)(FA-F-127/F-68-TPL). As a control, another drug delivery system based on a single polymer(FA-F-127-TPL) with a 1.90% drug-loading rate was prepared by substituting F-68 with F-127. The average particle sizes of FA-F-127/F-68-TPL and FA-F-127-TPL measured by a particle size analyzer were 30.7 nm and 31.6 nm, respectively. Their morphology was observed by atomic force microscopy(AFM). The results showed that FA-F-127-TPL self-assembled into nanomicelles, whereas FA-F-127/F-68-TPL self-assembled into nanogels. An MTT assay showed that a very low concentration of FA-F-127/F-68-TPL or FA-F-127-TPL could significantly inhibit the proliferation of multidrug-resistant(MDR) breast cancer cells(MCF-7/ADR cells) and induce cell death. The effects were significantly different from those of free TPL(P < 0.01). Using the fluorescent probe Nile red(Nr) as the drug model, FA-F-127/F-68-Nr nanogels and FAF-127-Nr nanomicelles were prepared and then incubated with human hepatocarcinoma(HepG2) and MCF-7/ADR cells, and the fluorescence intensity in the cells was measured by a multifunctional microplate reader. The results indicated that both FA-F-127/F-68-Nr and FA-F-127-Nr had sustained release in the cells, but HepG2 and MCF-7/ADR cells exhibited significantly higher endocytosis of FA-F-127/F-68-Nr than that of FA-F-127-Nr(P < 0.01). A nude mice transplanted tumor model was prepared to monitor FA-F-127/F-68-Nr in the tumor tissue and organs by whole-body fluorescent imaging. The results showed that FA-F-127/F-68-Nr targeted tumor tissues. The prepared nanogels had small particle size, were easy to swallow, exhibited slow release property,targeted tumor cells, and could improve the antitumor effects of TPL;hence, they are ideal carriers for low-dose antineoplastic drugs. | 尹美珍 SU Zhenhong CUI Bingcun HAN Yingchao DAI Honglian 喻昕 | 2019 | Journal of Wuhan University of Technology(Materials Science)2019，34，4： | 2 |
| 7 | EPMA Study of the Interface of β-Tricalcium Phosphate Ceramics in Vivo显示文摘Electron probe and X-ray energy spectrum were used to investigate the chemical composition of the interface between material and new bone after porous tricalcium phosphate ceramic implanted in tibia of rabbits. The element changes of the interface, the materials transformation and the situation of new bone formation at different implantation period were observed. The results showed that the carbon element content decreased gradually in new bone tissue, and the content of calcium and phosphor element increased by degrees with the implantation time. At the same time, calcium-phosphor ratio in the new bone kept a higher level. New bone grew into the materials interior, material dispersed and degraded simultaneously. Both composition of materials and new bone tended to be consentient. Finally, the materials were substituted by new bone. After implantation, not only the materials itself dissolved and degraded partially, but also new bone formed on the outer and pore surface of β-TCP porous bioceramics, which showed that the degradation products of lifeless calcium phosphate inorganic materials took part in constituting of new bone tissue. | Honglian DAI, Xianying CAO, Haichen SHAO, Chunhua SHEN and Shipu LIBiomedicaI Materials and Engineering Center, Wuhan University of Technology, Wuhan 430070, China | 2004 | Journal of Materials Science & Technology2004，20，6： | 1 |
| 8 | Promotion of peripheral nerve regeneration and prevention of neuroma formation by PRGD/PDLLA/b-TCP conduit:report of two cases显示文摘In the field of nerve repair,one major challenge is the formation of neuroma.However,reports on both the promotion of nerve regeneration and prevention of traumatic neuroma in the clinical settings are rare in the field of nerve repair.One of the reasons could be the insufficiency in the follow-up system.We have conducted 33 cases of nerve repair using PRGD/PDLLA/b-TCP conduit without any sign of adverse reaction,especially no neuroma formation.Among them,we have selected two cases as representatives to report in this article.The first case was a patient with an upper limb nerve wound was bridged by PRGD/PDLLA/b-TCP conduit and a plate fixation was given.After nearly 3-years’follow-up,the examination results demonstrated that nerve regeneration effect was very good.When the reoperation was performed to remove the steel plate we observed a uniform structure of the regenerated nerve without the formation of neuroma,and to our delight,the implanted conduit was completely degraded 23 months after the implantation.The second case had an obsolete nerve injury with neuroma formation.After removal of the neuroma,the nerve was bridged by PRGD/PDLLA/b-TCP conduit.Follow-up examinations showed that the structure and functional recovery were improved gradually in the 10-month follow-up;no end-enlargement and any other abnormal reaction associated with the characteristic of neuroma were found.Based on our 33-case studies,we have concluded that PRGD/PDLLA/b-TCP nerve conduit could both promote nerve regeneration and prevent neuroma formation;therefore,it is a good alternative for peripheral nerve repair. | Yixia Yin Binbin Li Qiongjiao Yan Honglian Dai Xinyu Wang Jifeng Huang Shipu Li | 2015 | Regenerative Biomaterials2015，2，2： | 1 |
| 9 | Synthesis and luminescence of Eu 3+ doped hydroxyapatite nanocrystallines: Effects of calcinations and Eu 3+ content显示文摘 | Yingchao Han Xinyu Wang Honglian Dai Shipu Li | 2013 | Journal of Luminescence2013，，： | 1 |
| 10 | Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway显示文摘There is a continuing need for artificial bone substitutes for bone repair and reconstruction,Magnesium phosphate bone cement(MPC)has exceptional degradable properties and exhibits promising biocompatibility.However,its mechanical strength needs improved and its low osteo-inductive potential limits its therapeutic application in bone regeneration.We functionally modified MPC by using a polymeric carboxymethyl chitosan-sodium alginate(CMCS/SA)gel network.This had the advantages of:improved compressive strength,ease of handling,and an optimized interface for bioactive bone in-growth.The new composites with 2%CMCS/SA showed the most favorable physicochemical properties,including mechanical strength,wash-out resistance,setting time,injectable time and heat release.Biologically,the composite promoted the attachment and proliferation of osteoblast cells.It was also found to induce osteogenic differentiation in vitro,as verified by expression of osteogenic markers.In terms of molecular mechanisms,data showed that new bone cement activated the Wnt pathway through inhibition of the phosphorylation ofβ-catenin,which is dependent on focal adhesion kinase.Through micro-computed tomography and histological analysis,we found that the MPC-CMCS/SA scaffolds,compared with MPC alone,showed increased bone regeneration in a rat calvarial defect model.Overall,our study suggested that the novel composite had potential to help repair critical bone defects in clinical practice. | Ling Yu Tian Gao Wei Li Jian Yang Yinchu Liu Yanan Zhao Ping He Xuefeng Li Weichun Guo Zhengfu Fan Honglian Dai | 2023 | Bioactive Materials2023，，2： | 1 |
| 11 | Bioactive calcium and mangnesium phosphate bone adhesive for enhanced vascularization and bone regeneration显示文摘Bone adhesive is a promising material for the treatment of bone fractures,which is helpful for the fast and effective reduction and fixation of broken bones.However,the existing adhesives bond weakly to bone tissues,and are non-absorbable,or hard to cure under wet conditions.Herein,inspired by the cement-based adhesive used in the industry field,we report a bioactive calcium and magnesium phosphate bone adhesive(MPBA)with the properties of facile preparation,robust adhesion,and bioactive.MPBA is equipped with similar strength to cancellous bones and shows reliable bonding performance for various interfaces,such as Ti6Al4V,Al2O3,and poly(ether-ether-ketone).MPBA achieves excellent bonding ability for the above interfaces with the bonding strengths of 2.28±0.47,2.32±0.15,and 1.44±0.38 MPa,respectively.Besides,it also shows reliable fixation ability for bovine bone surfaces.The bonding behavior to materials and bones suggests that MPBA could be used for both fracture treatment and implant fixation.Meanwhile,MPBA possesses good biological activity,which could promote the vascularization process and osteogenic differentiation.Finally,in vivo experiments confirmed MPBA can effectively restore bone strength and promote bone regeneration. | Jiawei Liu Honglei Kang Wenying Wei Rong Tu Takashi Goto Feng Li Honglian Dai | 2023 | Journal of Materials Science & Technology2023，，33： | 0 |
| 12 | Bioadaptable bioactive glass-β-tricalcium phosphate scaffolds with TPMS-gyroid structure by stereolithography for bone regeneration显示文摘Bone defect repair remains a troubling problem in clinical orthopedics,which involves complex biological processes.Calcium phosphates(CaPs)have been widely used owing to their advantage of biocompatibility.However,single component and traditional fabrication methods cannot meet the requirements of bioadaptability during the tissue repair process.In this work,0%,5%,15%,25%wt%of BG-TCP(bioactive glass-β-tricalcium phosphate)bioresorbable scaffolds with triply-periodic minimal surfaces(TPMS)-gyroid structure were prepared by the stereolithography(SLA)technology.TPMS-gyroid structure provided an accurate mimicry of natural bone tissue,and the incorporation of BG improved the compressive strength ofβ-TCP matrix,matched with the defective bone(2–12 MPa).Rapid but tunable degradation kinetics(compared with pure TCP)of BG enabled the BG-TCP system to sh8ow adaptable biodegradability to new bone generation.In vitro studies have shown that composite scaffolds have better mechanical properties(7.82 MPa),and can released appropriate contents of calcium,phosphorous,and magnesium ions,which promoted the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)and angiogenic ability of endothelial progenitor cells(EPCs).Moreover,the in vivo assessment of rat femoral defect revealed that TPMS-structure-based TCP scaffolds accelerated bone ingrowth to the pores.Moreover,BG-TCP scaffolds,especially 15BG-TCP group,exhibited superior bone regeneration capacity at both 4 and 8 weeks,which achieved an optimal match between the rate of material degradation and tissue regeneration.In summary,this study provides insight into influences of bioactive components(BG)and bionic structures(TPMS)on the physical-chemical properties of materials,cell behavior and tissue regeneration,which offers a promising strategy to design bioadaptive ceramic scaffolds in the clinical treatment of bone defects. | Meng Li Jiawei Jiang Wenbin Liu Xiaolong Huang Xiaopei Wu Wenying Wei Hao Zhu Jinyong Zhang Jun Xiao Honglian Dai | 2023 | Journal of Materials Science & Technology2023，，24： | 0 |
| 13 | Effects of β-TCP Ceramics on Osteoblast Cellular Proliferating,Mineralization and Osteocalcin Expression显示文摘After co-cultrured osteoblast with β-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that β-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that β-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that β-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess. | 齐志涛 张启焕 ZHENG Qiang DAI Honglian WANG Zisheng QIU Ming LI Shipu | 2012 | Journal of Wuhan University of Technology(Materials Science)2012，27，1： | 0 |
| 14 | RGD Gifted PDLLA-PRGD Conduits Promotes the Sciatic Nerve Regeneration显示文摘Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA films with RGD in vivo. The results revealed that RGD coating with the concentration of 100-500 ug/mL promoted the cell proliferation and boosted the cell migration. Molecularly, RGD coating also enhanced the expression of the proliferation related genes(c-fos and c-jun) and the cell behavior related genes(actin, tublin, tau and MAP1) at fi rst stages of the seeding, which is similar to the effects from laminin coating. In vivo, RGD addition improved the recovery effi ciency of the transected nerve in regard of the more survived Schwann cells in vivo and the formation of more mature myelin sheath. Taken together, RGD peptides are good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration by prompting responses in Schwann cells. | 方晓青 邱彤 XIE Lijuan YIN Yixia LI Binbin YAN Qiongjiao DAI Honglian WANG Xinyu LI Shipu | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，3： | 0 |
| 15 | Preparation and Characterization of Porous Calcium Phosphate Bioceramics显示文摘β-tricalcium phosphate (β-TCP) powder and Na2O-CaO-MgO-P2O5 glass binder were synthesized and mixed, and then the biodegradable porous calcium phosphate ceramics were successfully prepared by foaming and sintering at 8500C. The as-prepared ceramics possess a high porosity with partial three-dimension intercon- nected macro- and micro-pores. As in vitro experiment testified, the calcium phosphate ceramics (CPCs) has good degradability. | Honglian Dai Xinyu Wang Yinchao Han Xin Jiang Shipu Li | 2011 | Journal of Materials Science & Technology2011，27，5： | 0 |
| 16 | Design and fabrication of high-performance injectable self-setting trimagnesium phosphate显示文摘Magnesium phosphate bone cement has become a widely used orthopedic implant due to the advantages of fast-setting and high early strength. However, developing magnesium phosphate cement possessing applicable injectability, high strength, and biocompatibility simultaneously remains a significant challenge. Herein, we propose a strategy to develop high-performance bone cement and establish a trimagnesium phosphate cement (TMPC) system. The TMPC exhibits high early strength, low curing temperature, neutral pH, and excellent injectability, overcoming the critical limitations of recently studied magnesium phosphate cement. By monitoring the hydration pH value and electroconductivity, we demonstrate that the magnesium-to-phosphate ratio could manipulate the components of hydration products and their transformation by adjusting the pH of the system, which will influence the hydration speed. Further, the ratio could regulate the hydration network and the properties of TMPC. Moreover, in vitro studies show that TMPC has outstanding biocompatibility and bone-filling capacity. The facile preparation properties and these advantages of TMPC render it a potential clinical alternative to polymethylmethacrylate and calcium phosphate bone cement. This study will contribute to the rational design of high-performance bone cement. | Jiawei Liu Wen Hou Wenying Wei Jian Peng Xiaopei Wu Chenxi Lian Yanan Zhao Rong Tu Takashi Goto Honglian Dai | 2023 | Bioactive Materials2023，，10： | 0 |
| 17 | Preparation and Drug-release Behavior of β-TCP Ceramics Drug Carrier in vitro显示文摘β-TCP ceramics drug carrier was first prepared and characterized. SEM showed that β-TCP carrier was in porous amorphous structure with diameters around 10 μm. The physical properties including apparent porosity, volume-weight, tensile strength and the permeability were measured and the results indicated those properties fit the clinical usage of β-TCP drug carrier. Furthermore, drug release experiment in vitro showed that the carrier could prolong drug release in simulated body fluid which provides basis for the clinical use of β-TCP ceramics as drug carrier. | 张启焕 YAN Xin YAN Yuhua DAI Honglian JIANG Xin LI Shipu | 2012 | Journal of Wuhan University of Technology(Materials Science)2012，27，6： | 0 |
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