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| 1 | H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression显示文摘AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents. | Valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa André Márcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst | 2008 | World Journal of Gastroenterology2008，14，6： | 17 |
| 2 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 3 | Diffuse intestinal ganglioneuromatosis an uncommon manifestation of Cowden syndrome显示文摘Diffuse intestinal ganglioneuromatosis is a hamartomatous polyposis characterized by a disseminated, intramural or transmural proliferation of neural elements involving the enteric plexuses. It has been associated with MEN Ⅱ, neurofibromatosis type 1 and hamartomatous polyposis associated with phosphatase and tensin homolog mutation. We report the case of a female patient with a history of a breast and endometrial tumor who presented in a colonoscopy performed for rectal bleeding diffuse ganglioneuromatosis, which oriented the search for other characteristic findings of Cowden syndrome given the personal history of the patient. The presence of an esophagogastric polyposis was also noted. Cowden syndrome is characterized by skin lesions, but it is rarely diagnosed by these lesions, because they are usually overlooked. Intestinal polyposis is not a major diagnostic criterion but it is very useful for early diagnosis. The combination of colonic polyposis and glucogenic acanthosis should orient the diagnosis to Cowden syndrome. | Maria Teresa Herranz Bachiller Jesus Barrio Andrés Fernando Pons Noelia Alcaide Suárez Rafael Ruiz-Zorrilla Lorena Sancho del Val Sara Lorenzo Pelayo Carlos De La Serna Higuera Ramon Atienza Sánchez Manuel Perez Miranda | 2013 | World Journal of Gastrointestinal Oncology2013，5，2： | 5 |
| 4 | The right ventricle, outflow tract, and ventricular septum comprise a restricted expression domain within the secondary/anterior heart field 显示文摘 | Verzi MP McCulley DJ De Val S | 2005 | Dev Biol2005，287，1： | 1 |
| 5 | Transcriptional control of endothelial cell development 显示文摘 | De VAL S BLACK BL | 2009 | Dev Cell2009，16，： | 1 |
| 6 | Inactivation of COX-2, HMLH1 and CDKN2A Gene by Promoter Methylation in Gastric Cancer: Relationship with Histological Subtype, Tumor Location and Helicobacter pylori Genotype显示文摘 | Alves Markênia Kélia Santos Ferrasi Adriana Camargo Lima Valeska Portela Ferreira Márcia Valéria Pitombeira De Moura Campos Pardini Maria Inês Rabenhorst Silvia Helena Barem | 2011 | EN2011，，5： | 1 |
| 7 | Assessment and detection of pain in noncommunicative severely brain-injured patients显示文摘 | Schnakers C Chatelle (3 Majerus S C osseries O De Val M Laureys S | 2010 | Expert Rev Neurother2010，10，11： | 1 |
| 8 | The right ventricle,outflow tract,and ventricular septum comprise a restricted expression domain within the secondary/anterior heart field显示文摘 | VERZI M P MCCULLEY D J DE VAL S | 2005 | Dev Biol2005，287，1： | 1 |
| 9 | In vivo enhancer analysis of human conserved non-coding sequences显示文摘 | Pennacchio LA Ahituv N Moses AM Prabhakar S Nobrega MA Shoukry M Minovitsky S Dubchak I Holt A Lewis KD Plajzer-Frick I Akiyama J De Val S Afzal V Black BL Couronne O Eisen MB Visel A Rubin EM | | 0，，7118： | 1 |
| 10 | Rubber tree clones with resistance to mites显示文摘 | Silva HA de S da Vieira MR Valério Filho WV | | 0，，02： | 1 |
| 11 | Hydrolysis of oligosaccharides in soybean products by Debaryomyces hansenii UFV-1 α-galactosidases显示文摘 | Pollyanna Amaral Viana Sebasti?o Tavares de Rezende Daniel Luciano Falkoski Thiago de Almeida Leite Inês Chamel José Maurílio Alves Moreira Valéria Monteze Guimar?es | 2006 | Food Chemistry2006，，2： | 1 |
| 12 | Arachidonylethanolamide Induces Apoptosis of Human Glioma Cells through Vanilloid Receptor-1显示文摘 | Emmanuel C Rick W Mirna T Belkouch MC Valérie S Nicolas de T | 2004 | J Neuropathol Exp Neurol2004，63，： | 1 |
| 13 | Combinatorial regulation of endothelial gene express and forkhead tranSCription factors显示文摘 | De Val S Chi NC Meadows SM | 2008 | Cell2008，135，6： | 1 |
| 14 | The right ventricle, outflow tract, and ventricular septum comprise a restricted ex- pression domain within the secondary/anterior heart field显示文摘 | Verzi M P McCuUey D J De Val S | 2005 | Dev Biol2005，287，1： | 1 |
| 15 | The right ventricle, outflow tract, and ventricular septum comprise a restricted expression domain within the secondary/anterior heart field 显示文摘 | Verzi MP McCulley DJ De Val S | 2005 | Dev Biol2005，287，1： | 1 |
| 16 | Practices and perception of risk in human immunodeficiency virus infected males who have sex with other males 显示文摘 | Fernandez de Mosteyrin S del Val Acebr6n M Fernindez de Mosteyrin T | 2014 | Enferm Infecc Microbiol Clin2014，32，4： | 1 |
| 17 | Foxn4 directly regulates tbx2b expression and atrioventricular canal formation显示文摘 | CHIN C SHAW R M DE VAL S | | 0，，06： | 1 |
| 18 | Continuous spinal anesthesia:a comparative study of standard microcather and Spinocath显示文摘 | De Andrés J Valía JC Olivares A | 1999 | Reg Anesth Pain Med1999，24，2： | 1 |
| 19 | The right ventricle,outflow tract,and ventricular septum comprise a restricted expression domain within the secondary/anterior heart field显示文摘 | Verzi M P McCulley D J De Val S | 2005 | Dev Biol2005，287，1： | 1 |
| 20 | Combinatorial regulation of endothelial gene expression by ets and fork- head transcription factors 显示文摘 | De Val S Chi NC Meadows SM et ai | 2008 | Cell2008，135，： | 1 |
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