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| 1 | H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression显示文摘AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents. | Valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa André Márcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst | 2008 | World Journal of Gastroenterology2008，14，6： | 17 |
| 2 | Interrelationship between chromosome 8 aneuploidy,C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma显示文摘AIM: To investigate chromosome 8 numerical aberra- tions, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histo- pathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immu- nostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneu- ploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level ofchromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal- type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic path- ways. | Danielle Queiroz Calcagno Mariana Ferreira Leal Aline Damaceno Seabra Andre Salim Khayat Elizabeth Suchi Chen Samia Demachki Paulo Pimentel Assumpcao Mario Henrique Girao Faria Silvia Helena Barem Rabenhorst Márcia Valéria Pitombeira Ferreira Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano | 2006 | World Journal of Gastroenterology2006，12，38： | 9 |
| 3 | Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures显示文摘Background: Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments: control, which received no supplement; supplementation for the last 30 d of gestation(30-d; 3.0 kg/d); supplementation for the last 60 d of gestation(60-d; 1.5 kg/d); or supplementation for the last 90 d of gestation(90-d; 1.0 kg/d). All supplemented treatments received the same total amount of supplement throughout the experiment: 90 kg(20% of crude protein). A second experiment(Experiment 2) was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d each.Results: There was a linear effect of the number of days of supplementation on calving body weight(BW; P < 0.05)and a quadratic effect on BW change from parturition to d 31 post-calving(P < 0.05), with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW(P > 0.10). A significant linear effect on interval from parturition to conception(P < 0.05) was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility(P > 0.10). Nitrogen excreted through urine tended to increase linearly with the level of supplementation(P < 0.10).Conclusion: Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of days from calving to re-conception in the following breeding season compared to the usually recommended period of supplementation of 90 d pre-partum. | Aline Gomes da Silva Mário Fonseca Paulino Edenio Detmann Henrique Jorge Fernandes Lincoln da Silva Amorim Román Enrique Maza Ortega Victor Valério de Carvalho Josilaine Aparecida da Costa Lima Felipe Henrique de Moura Mariana Benevides Monteiro Jéssika Almeida Bitencourt | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 2 |
| 4 | Expression analysis of the Ara-bidopsis peroxidase multigenic family显示文摘 | VALéRIO L DE MEYER M PENEL C | 2004 | Phytochemistry2004，65，10： | 1 |
| 5 | Degradation of polypropylene (PP) during multiple extrusions:Thermal analysis,mechanical properties and analysis of variance显示文摘 | Helson M da Costa Valé ria D Ramos Márcia G de Oliveira | | 0，，： | 1 |
| 6 | Expressionanalysis of the Arabidopsis peroxidase multigenic family显示文摘 | VAL RIO L DE MEYER M PENEL C | 2004 | Phytochemistry2004，65，10： | 1 |
| 7 | Inactivation of COX-2, HMLH1 and CDKN2A Gene by Promoter Methylation in Gastric Cancer: Relationship with Histological Subtype, Tumor Location and Helicobacter pylori Genotype显示文摘 | Alves Markênia Kélia Santos Ferrasi Adriana Camargo Lima Valeska Portela Ferreira Márcia Valéria Pitombeira De Moura Campos Pardini Maria Inês Rabenhorst Silvia Helena Barem | 2011 | EN2011，，5： | 1 |
| 8 | Degradation of polypropylene (PP) during multiple extrusions:Thermal analysis,mechanical properties and analysis of variance显示文摘 | Helson M da Costa Valé ria D Ramos M á rcia G de Oliveira | | 0，，： | 1 |
| 9 | Assessment and detection of pain in noncommunicative severely brain-injured patients显示文摘 | Schnakers C Chatelle (3 Majerus S C osseries O De Val M Laureys S | 2010 | Expert Rev Neurother2010，10，11： | 1 |
| 10 | The right ventricle,outflow tract,and ventricular septum comprise a restricted expression domain within the secondary/anterior heart field显示文摘 | VERZI M P MCCULLEY D J DE VAL S | 2005 | Dev Biol2005，287，1： | 1 |
| 11 | In vivo enhancer analysis of human conserved non-coding sequences显示文摘 | Pennacchio LA Ahituv N Moses AM Prabhakar S Nobrega MA Shoukry M Minovitsky S Dubchak I Holt A Lewis KD Plajzer-Frick I Akiyama J De Val S Afzal V Black BL Couronne O Eisen MB Visel A Rubin EM | | 0，，7118： | 1 |
| 12 | Barleyβ-glucans extraction and partial characterization显示文摘 | Valéria M Limberger-Bayer Alicia de Francisco | 2014 | Food Chemistry2014，154，： | 1 |
| 13 | Practices and perception of risk in human immtmodeficiency virus infected males who have sex with other males 显示文摘 | De Mosreyrln SF Del Val Acebr6n M De Mosteyrln TF | 2014 | Enferm Infecc Microbiol Clin2014，32，4： | 1 |
| 14 | The right ventricle, outflow tract, and ventricular septum comprise a restricted ex- pression domain within the secondary/anterior heart field显示文摘 | Verzi M P McCuUey D J De Val S | 2005 | Dev Biol2005，287，1： | 1 |
| 15 | Protective immunization against murine cytomegalovirus infection using adenoviruses and poxviruses expressing hepatitis B virus chimeras 显示文摘 | de Andrade BP Gazzinelli RT Del Val M | 2007 | Int Microbiol2007，10，： | 1 |
| 16 | Practices and perception of risk in human immunodeficiency virus infected males who have sex with other males 显示文摘 | Fernandez de Mosteyrin S del Val Acebr6n M Fernindez de Mosteyrin T | 2014 | Enferm Infecc Microbiol Clin2014，32，4： | 1 |
| 17 | Foxn4 directly regulates tbx2b expression and atrioventricular canal formation显示文摘 | CHIN C SHAW R M DE VAL S | | 0，，06： | 1 |
| 18 | Expression Analysis of the Arabidopsis Peroxidase Multigenic Family显示文摘 | Valério L De Meyer M Penel C | 2004 | Phytochemistry2004，65，10： | 1 |
| 19 | The right ventricle,outflow tract,and ventricular septum comprise a restricted expression domain within the secondary/anterior heart field显示文摘 | Verzi M P McCulley D J De Val S | 2005 | Dev Biol2005，287，1： | 1 |
| 20 | Expression analysis of the Arabidopsis peroxidase multigenic family显示文摘 | Val rio L De Meyer M Penel C | 2004 | Phytochemistry2004，65，10： | 1 |
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