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| 1 | Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.)显示文摘A narrow leaf mutant was obtained after T-DNA transformation conducted on a rice variety Zhonghua 11. Several abnormal morphological characteristics, including semi-dwarf, delayed flowering time, narrow and inward rolling leaves, and lower seed-setting, were observed. The rate of net photosynthesis (un-der saturate light) of flag leaves in the mutant was significantly lower than that of the wild type. More-over, the leaf transpiration rate and stomatal conductance in the mutant flag leaf were lower than those of the wild type at the grain filling stage. It was found that the mutant phenotype was not caused by the T-DNA insertion. Genetic analysis showed that the mutant was controlled by a single recessive gene, designated as nal3(t). A genetic linkage map was constructed using a large F2 mapping population de-rived from a cross between nal3(t) and an indica variety Longtefu B with 6 polymorphic markers on chromosome 12 identified from 366 SSR markers by the BAS method. Gene nal3(t) was mapped be-tween the markers RM7018 and RM3331. Fine mapping of nal3(t) locus was conducted with 22 newly developed STS markers based on the sequence diversity around the region harboring nal3(t) between Nipponbare and 93-11, and nal3(t) was finally mapped to a 136-kb region between the STS markers NS10 and RH12-8. | WANG DeKai LIU HeQin LI KeLei LI SuJuan TAO YueZhi | 2009 | Chinese Science Bulletin2009，54，5： | 19 |
| 2 | The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice显示文摘Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question.However,the molecular mechanisms by which plants determine their seed size remain elusive.Here,we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice.WG1,which encodes a glutaredoxin protein,promotes grain growth by increasing cell proliferation.Interestingly,WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1,indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor.In contrary,OsbZIP47 restricts grain growth by decreasing cell proliferation.Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation.Genetic analyses confirm that GW2,WG1,and OsbZIP47 function in a comm on pathway to control grain growth.Taken together,ourfindi ngs reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module,providing new targets for improving seed size and weight in crops. | Jianqin Hao Dekai Wang Yingbao Wu Ke Huang Penggen Duan Na Li Ran Xu Dali Zeng Guojun Dong Baolan Zhang Limin Zhang Dirk Inze Qian Qian Yunhai Li | 2021 | Molecular Plant2021，14，8： | 14 |
| 3 | The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells Superior effects over original formula of Buyang Huanwu decoction显示文摘The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction (active principle region of decoction for invigorating yang for recuperation). After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula. | Jinghui Zheng Yi Wan Jianhuai Chi Dekai Shen Tingting Wu Weimin Li Pengcheng Du | 2012 | Neural Regeneration Research2012，7，4： | 9 |
| 4 | OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development显示文摘Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice(Oryza sativa) mutant that exhibited albino and seedling-lethal phenotypes was isolated from a60Co-irradiated rice population. The mutant gene was identified as an ortholog of the Arabidopsis plastid transcriptionally active chromosome protein 2(p TAC2) gene, and the mutant strain was designated osptac2. Sequence and transcription analyses showed that Osp TAC2 encodes a putative chloroplast protein consisting of 10 pentratricopeptide repeat(PPR) domains and a C-terminal small Mut S-related(SMR) domain. Cytological observations via microscopy showed that the Osp TAC2-green fluorescent fusion protein is localized in the chloroplasts. Transmission electron microscopy revealed that the chloroplast of the osptac2 mutant lacks an organized thylakoid membrane. The transcript levels of all investigated PEP(plastid-encoded RNA polymerase)-dependent genes were dramatically reduced in the osptac2 mutant, whereas the transcript levels of NEP(nuclear-encoded polymerase)-dependent genes were increased. These results suggest that Osp TAC2 plays a critical role in chloroplast development and indicate that the molecular function of the Osp TAC2 gene is conserved in rice and Arabidopsis. | Dekai Wang Heqin Liu Guowei Zhai Liangsheng Wang Jianfeng Shao Yuezhi Tao | 2016 | Journal of Genetics and Genomics2016，43，10： | 7 |
| 5 | Novel trichromatic phosphor Co-doped with Eu, Tb in SiO_2 gel matrix显示文摘The Eu, Tb co-doped SiO2 matrix tricolor fluorescence system was prepared by sol-gel technique. Red emission at 618 nm, green emission at 543 nm and blue emission at 350―500 nm were observed in the PL spectra of the sample, indicating that Eu3+, Eu2+ and Tb3+ ions coexisted in the matrix. In the co-doped sample, the blue emission of Eu2+ was much stronger than that of the sample single doped with Eu, which implied that the electron transfer between Eu3+ and Tb3+ maybe happened in the SiO2 matrix. The influences of the annealing temperature and Tb concentration on the PL spectra of the samples were investigated. The optimal doped concentration of Tb was determined to be 0.2% and the optimal annealing temperature 850℃. Annealed at 600℃, Tb3+ had a sensitizing effect on Eu3+ in the SiO2 matrix, and the emission intensity of Eu3+ in the Eu, Tb co-doped sample was more than four times that of the single doped sample, which could be attributed to the energy transfer from Tb3+ to Eu3+. | HU XiaoYun FAN Jun LI Ting ZHANG DeKai BAI Jinmao REN ZhaoYu HOU Xun | 2007 | Chinese Science Bulletin2007，52，4： | 5 |
| 6 | Organellar genome assembly methods and comparative analysis of horticultural plants显示文摘Although organellar genomes(including chloroplast and mitochondrial genomes)are smaller than nuclear genomes in size and gene number,organellar genomes are very important for the investigation of plant evolution and molecular ecology mechanisms.Few studies have focused on the organellar genomes of horticultural plants.Approximately 1193 chloroplast genomes and 199 mitochondrial genomes of land plants are available in the National Center for Biotechnology Information(NCBI),of which only 39 are from horticultural plants.In this paper,we report an innovative and efficient method for high-quality horticultural organellar genome assembly from next-generation sequencing(NGS)data.Sequencing reads were first assembled by Newbler,Amos,and Minimus software with default parameters.The remaining gaps were then filled through BLASTN search and PCR.The complete DNA sequence was corrected based on Illumina sequencing data using BWA(Burrows–Wheeler Alignment tool)software.The advantage of this approach is that there is no need to isolate organellar DNA from total DNA during sample preparation.Using this procedure,the complete mitochondrial and chloroplast genomes of an ornamental plant,Salix suchowensis,and a fruit tree,Ziziphus jujuba,were identified.This study shows that horticultural plants have similar mitochondrial and chloroplast sequence organization to other seed plants.Most horticultural plants demonstrate a slight bias toward A+T rich features in the mitochondrial genome.In addition,a phylogenetic analysis of 39 horticultural plants based on 15 protein-coding genes showed that some mitochondrial genes are horizontally transferred from chloroplast DNA.Our study will provide an important reference for organellar genome assembly in other horticultural plants.Furthermore,phylogenetic analysis of the organellar genomes of horticultural plants could accurately clarify the unanticipated relationships among these plants. | Xuelin Wang Feng Cheng Dekai Rohlsen Changwei Bi Chunyan Wang Yiqing Xu Suyun Wei Qiaolin Ye Tongming Yin Ning Ye | 2018 | Horticulture Research2018，5，1： | 4 |
| 7 | Electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in the injured spinal cord of adult rats显示文摘A contusive model of spinal cord injury at spinal segment T8-9 was established in rats. Huantiao (GB30) and Huatuojiaji (Ex-B05) were punctured with needles, and endogenous neural stem cells were labeled with 5-bromo-2'-deoxyuridine (BrdU) and NG2. Double immunofluorescence staining showed that electroacupuncture markedly increased the numbers of BrdU+/NG2+ cells at spinal cord tissue 15 mm away from the injury center in the rostral and caudal directions. The results suggest that electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in rats with spinal cord injury. | Haiying Wu Min HU Dekai Yuan Yunhui Wang Jing Wang Tao Li Chuanyun Qian Hualin YU | 2012 | Neural Regeneration Research2012，7，15： | 4 |
| 8 | Enhanced Light-Harvesting Efficiency and Adaptation: A Review on Visible-Light-Driven Micro/Nanomotors显示文摘As visible light accounts for a larger proportion of solar energy and is harmless to living organisms,it has the potential to be the energy source of micro/nanomotors,which transform visible-light energy into mechanical motion,for different applications,especially in environmental remediation.However,how to precisely control the motion of visible-light-driven micro/nanomotors(VLD-MNMs)and efficiently utilize the weak visible-light photon energy to acquire rapid motion are significant challenges.This review summarizes the most critical aspects,involving photoactive materials,propulsion mechanisms,control methods,and applications of VLD-MNMs,and discusses strategies to systematically enhance the energyharvesting efficiency and adaptation.At first,the photoactive materials have been divided into inorganic and organic photoactive materials and comprehensively discussed.Then,different propulsion mechanisms of the current VLD-MNMs are presented to explain the improvement in the actuation force,speed,and environmental adaptability.In addition,considering the characteristics of easy control of VLD-MNMs,we summarized the direction,speed,and cluster control methods of VLD-MNMs for different application requirements.Subsequently,the potential applications of VLD-MNMs,e.g.,in environmental remediation,micropumps,cargo delivery,and sensing in microscale,are presented.Finally,discussions and suggestions for future directions to enhance the energy-harvesting efficiency and adaptation of VLD-MNMs are provided. | Dekai Zhou Rencheng Zhuang Xiaocong Chang Longqiu Li | 2020 | Research2020，，1： | 3 |
| 9 | Characterization and molecular cloning of a serine hydroxymethyltransferase 1(OsSHM_1) in rice显示文摘Serine hydroxymethyltransferase(SHMT) is important for one carbon metabolism and photorespiration in higher plants for its participation in plant growth and development,and resistance to biotic and abiotic stresses. A rice serine hydroxymethyltransferase gene, Os SHM1, an ortholog of Arabidopsis SHM1, was isolated using map-based cloning. The osshm1 mutant had chlorotic lesions and a considerably smaller,lethal phenotype under natural ambient CO2 concentrations,but could be restored to wild type with normal growth under elevated CO2levels(0.5% CO2), showing a typical photorespiratory phenotype. The data from antioxidant enzymes activity measurement suggested that osshm1 was subjected to signi fi cant oxidative stress. Also, Os SHM1 was expressed in allorgans tested(root, culm, leaf, and young panicle) but predominantly in leaves. Os SHM1 protein is localized to the mitochondria. Our study suggested that molecular function of the Os SHM1 gene is conserved in rice and Arabidopsis. | Dekai Wang Heqin Liu Sujuan Li Guowei Zhai Jianfeng Shao Yuezhi Tao | 2015 | Journal of Integrative Plant Biology2015，57，9： | 3 |
| 10 | Epigenetic considerations and the clinical reevaluation of the overlap syndrome between primary biliary cirrhosis and autoimmune hepatitis显示文摘 | Qixia Wang Carlo Selmi Xinmin Zhou Dekai Qiu Zenshan Li Qi Miao Xiaoyu Chen Jianhong Wang Edward L. Krawitt M. Eric Gershwin Ying Han Xiong Ma | 2012 | Journal of Autoimmunity2012，，： | 2 |
| 11 | Validation of the simplified criteria for diagnosis of autoimmune hepatitis in Chinese patients显示文摘 | Dekai Qiu Qixia Wang Hui Wang Qing Xie Guoqing Zang Hong Jiang Chuantao Tu Jinsheng Guo Shuncai Zhang Jianshe Wang Yi Lu Ying Han Lei Shen Xiaoyu Chen Xiqi Hu Xiaojin Wang Chengwei Chen Qingchun Fu Xiong Ma | 2010 | Journal of Hepatology2010，，： | 2 |
| 12 | Predicting loss of life in case of dam failure and flash flood 显示文摘 | Dekay M L McClelland G H | 1993 | Risk Analysis1993，，2： | 1 |
| 13 | TLR11 Activation of dendritic cells by a protozoan profilin-like protein显示文摘 | Felix Y Dekai Z John F A | 2005 | Science2005，308，5728： | 1 |
| 14 | Predicting loss of life in cases of dam failure and flash flood显示文摘 | DEKAY M L MCCLELLANDL G H | 1993 | Risk Analysis1993，13，2： | 1 |
| 15 | Synthesis and assay of isoquinoline derivative5 as HIV 1 Tat-TAR interaction inhibitors 显示文摘 | He Meizi Yuan Dekai Yang Ming | 2005 | Bioorganic & Medicinal Chemistry Letter2005，15，： | 1 |
| 16 | Predicting loss of life in case of dam failure and flash flood显示文摘 | McClelland G H | 1993 | Risk Analysis1993，，2： | 1 |
| 17 | Creation of fragrant sorghum by CRISPR/Cas9显示文摘Sorghum,the fifth largest cereal crop,has high value as a staple food and raw material for liquor and vinegar brewing.Due to its high biomass and quality,it is also used as the second most planted silage resource.No fragrant sorghums are currently on the market.Through CRISPR/Cas9-mediated knockout of Sb BADH2,we obtained sorghum lines with extraordinary aromatic smell in both seeds and leaves.Animal feeding experiments showed that fragrant sorghum leaves were attractable.We believe this advantage will produce great value in the sorghum market for both grain and whole biomass forage. | Dan Zhang Sanyuan Tang Peng Xie Dekai Yang Yaorong Wu Shujing Cheng Kai Du Peiyong Xin Jinfang Chu Feifei Yu Qi Xie | 2022 | Journal of Integrative Plant Biology2022，64，5： | 1 |
| 18 | Predicting Loss of Lifein Cases of Dam Failure and Flash Flood显示文摘 | DEKAY M L MCCLELLAND G H | 1993 | Risk Analysis1993，13，2： | 1 |
| 19 | Predicting loss of life in cases of dam failure and flash flood显示文摘 | DEKAY M L MCCLELLAND G H | 1993 | Risk Analysis1993，13，2： | 1 |
| 20 | Stochastic inversion transduction grammars and bilingual parsing of parallel corpora显示文摘 | Dekai WU | 1997 | Computational Linguistics1997，23，3： | 1 |
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