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| 1 | Solution structure of the RNA recognition domain of METTL3-METTL14 N^6-methyladenosine methyltransferase显示文摘N^6-methyladenosine(m6A),a ubiquitous RNA modification,is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between the methyltransferase domains(MTDs)of METTL3 and METTL14 has been previously determined.However,the MTDs alone possess no enzymatic activity.Here we present the solution structure for the zinc finger domain(ZFD)of METTL3,the inclusion of which fulfills the methyltransferase activity of METTL3-METTL14.We show that the ZFD specifically binds to an RNA containing 5'-GGACU-3'consensus sequence,but does not to one without.The ZFD thus serves as the target recognition domain,a structural feature previously shown for DNA methyltransferases,and cooperates with the MTDs of METTL3-METTL14 for catalysis.However,the interaction between the ZFD and the specific RNA is extremely weak,with the binding affinity at several hundred micromolar under physiological conditions.The ZFD contains two CCCH-type zinc fingers connected by an anti-parallel P-sheet.Mutational analysis and NMR titrations have mapped the functional interface to a contiguous surface.As a division of labor,the RNA-binding interface comprises basic residues from zinc finger 1 and hydrophobic residues fromβ-sheet and zinc finger 2.Further we show that the linker between the ZFD and MTD of METTL3 is flexible but partially folded,which may permit the cooperation between the two domains during catalysis.Together,the structural characterization of METTL3 ZFD paves the way to elucidate the atomic details of the entire process of RNA m6A modification. | Jinbo Huang Xu Dong Zhou Gong Ling-Yun Qin Shuai Yang Yue-Ling Zhu Xiang Wang Delin Zhang Tingting Zou Ping Yin Chun Tang | 2019 | Protein & Cell2019，10，4： | 11 |
| 2 | A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var.beijingensis显示文摘The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi.It is one of the important effective components in Agricultural Antibiotic120,which has been widely used as naturally-originated agents for treatment of crop decay in China.The chemical synthesis of anisomycin has recently been reported,but the complex process with low productivity made the biosynthesis still to be a vital mainstay in efforts.The biosynthetic gene cluster(BGC)of anisomycin in Streptomyces hygrospinosus var.beijingensis has been identified in our previous work,while poor understanding of the regulatory mechanism limited the yield enhancement via regulation engineering of S.hygrospinosus var.beijingensis.In this study here,we characterized AniF as an indispensable LuxR family transcriptional regulator for the activation of anisomycin biosynthesis.The genetic manipulations of aniF and the real-time quantitative PCR(RT-qPCR)revealed that it positively regulated the transcription of the anisomycin BGC.Moreover,the overexpression of aniF contributed to the improvement of the production of anisomycin and its derivatives.Dissection of the mechanism underlying the function of AniF revealed that it directly activated the transcription of the genes aniR-G involved in anisomycin biosynthesis.Especially,one AniF-binding site in the promoter region of aniR was identified by DNase I footprinting assay and an inverted repeat sequence(5′-GGGC-3′)composed of two 4-nt half sites in the protected region was found.Taken together,our systematic study confirmed the positive regulatory role of AniF and might facilitate the future construction of engineering strains with high productivity of anisomycin and its derivatives. | Jufang Shen Lingxin Kong Yan Li Xiaoqing Zheng Qing Wang Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 10 |
| 3 | O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an “O-Follow-N” rule显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2),the causative agent of coronavirus disease 2019(COVID-19),emerged in late 2019 and has since caused a pandemic.Although there have been extensive studies worldwide,our understanding of this newly emerged pathogen is far from sufficient.The pathogenesis of the SARS-CoV-2 infection is not fully understood,although a“two-stage”hypothesis was proposed in our previous study. | Wenmin Tian Delin Li Nan Zhang Guijie Bai Kai Yuan Haixia Xiao Feng Gao Yang Chen Catherine C.L.Wong George Fu Gao | 2021 | Cell Research2021，31，10： | 9 |
| 4 | Target-enclosing affine formation control of two-layer networked spacecraft with collision avoidance显示文摘This paper addresses a target-enclosing problem for multiple spacecraft systems by proposing a two-layer affine formation control strategy. Compared with the existing methods,the adopted two-layer network structure in this paper is generally directed, which is suitable for practical space missions. Firstly, distributed finite-time sliding-mode estimators and formation controllers in both layers are designed separately to improve the flexibility of the formation control system. By introducing the properties of affine transformation into formation control protocol design,the controllers can be used to track different time-varying target formation patterns. Besides, multilayer time-varying encirclements can be achieved with particular shapes to surround the moving target. In the sequel, by integrating adaptive neural networks and specialized artificial potential functions into backstepping controllers, the problems of uncertain Euler-Lagrange models, collision avoidance as well as formation reconfiguration are solved simultaneously. The stability of the proposed controllers is verified by the Lyapunov direct method. Finally, two simulation examples of triangle formation and more complex hexagon formation are presented to illustrate the feasibility of the theoretical results. | Yang XU Delin LUO Dongyu LI Yancheng YOU Haibin DUAN | 2019 | Chinese Journal of Aeronautics2019，32，12： | 9 |
| 5 | Genetic Mapping of Laminaria japonica and L. Iongissima Using Amplified Fragment Length Polymorphism Markers in a "Two-Way Pseudo- Testcross" Strategy显示文摘 | Yuhui Li Yingxia Yang Jidong Liu Xiuliang Wang Tianxiang Gao Delin Duan | 2007 | Journal of Integrative Plant Biology2007，49，3： | 8 |
| 6 | Affine formation maneuver tracking control of multiple second-order agents with time-varying delays显示文摘This paper considers an affine maneuver tracking control problem for leader-follower type second-order multi-agent systems in the presence of time-varying delays, where the interaction topology is directed. Using the property of the affine transformation,this paper gives the sufficient and necessary conditions of achieving the affine localizability and extends it to the second-order condition. Under the(n + 1)-reachable condition of the given n-dimensional nominal formation with n + 1 leaders, a formation of agents can be reshaped in arbitrary dimension by only controlling these leaders. When the neighboring positions and velocities are available, a formation maneuver tracking control protocol with time-varying delays is constructed with the form of linear systems, where the tracking errors of the followers can be specified. Based on Lyapunov-Krasovskii stability theory, sufficient conditions to realize affine maneuvers are proposed and proved, and the unknown control gain matrix can be solved only by four linear matrix inequalities independent of the number of agents. Finally, corresponding simulations are carried out to verify the theoretical results, which show that these followers can track the time-varying references accurately and continuously. | XU Yang LI DongYu LUO DeLin YOU YanCheng | 2019 | Science China(Technological Sciences)2019，62，4： | 5 |
| 7 | Characterization of the positive SARP family regulator PieR for improving piericidin A1 production in Streptomyces piomogeues var.Hangzhouwanensis显示文摘Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity. | Yan Li Lingxin Kong Jufang Shen Qing Wang Qian Liu Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 4 |
| 8 | New advances in multiple autonomous aerial robots formation control technology显示文摘Autonomous aerial robotics has become a hot direction ofresearch inside the community of robotics and control. Theprimary problem addressed by formation control is to steermultiple aerial robots to form desired geometric patterns and,at the same time, realize desired collective swarming behaviorsin a decentralized or distributed manner. In contrast toground vehicles, aerial robots have the ability to work inthree-dimensional (3D) airspace. Equipped with electric orhydraulic motors, the vertical take-off and landing (VTOL)capability is a typical performance of aerial robots. Formationcontrol technology for such aerial robots is incessantlyspringing up to satisfy the requirements of highly intelligentautonomous systems, which affects both military and civilareas, including missile defense, battlefield surveillance,satellite network construction, fire suppression, power gridinspection, commercial show, etc. [1–5]. Such a problem ofmultiple aerial robots formation control is exceptionallychallenging to analyze if practical constraints such as complexdynamics, motion constraints, and imperfect measurementsare incorporated. | XU Yang LUO DeLin YOU YanCheng DUAN HaiBin | 2019 | Science China(Technological Sciences)2019，62，10： | 3 |
| 9 | Affine transformation based formation maneuvering for discrete-time directed networked systems显示文摘For the tracking control problems of affine formation maneuvers with discrete leader-following multi-agent systems, various distributed control schemes based on the directed graphs are designed in this paper. Consider the single and double-integrator models, different types of formation maneuvers can be achieved subject to the states of leaders with the novel characteristic of affine localizability. Meanwhile, the properties of affine transformation and signed Laplacian are introduced, which simplify the structures of controllers with distinct constraints to a great extent. Moreover, the convergence analysis of proposed protocols is proven. Static, constant and time-varying target formations can be tracked successfully. In the end, corresponding numerical simulations are carried out to certify the validity of theoretical results. | XU Yang LUO DeLin YOU YanCheng DUAN HaiBin | 2020 | Science China(Technological Sciences)2020，63，1： | 3 |
| 10 | Distributed planar formation maneuvering of leader-follower networked systems via a barycentric coordinate-based approach显示文摘This paper presents a distributed planar leader-follower formation maneuver control strategy for multi-agent systems with different agent dynamic models.This method is based on the barycentric coordinate-based(BCB)control,which can be performed in the local coordinate frame of each agent with required local measurements.By exploring the properties of BCB Laplacians,a time-varying target formation can be BCB localizable by a sufficient number of leaders uniquely,and this formation is converted from a given nominal formation with geometrical similarity transformation.The proposed control laws can continuously maneuver collective single-and double-integrator agents to achieve a translation,scale,rotation,or even their compositions in various directions.For the formation shape control problem of multi-car systems with/without saturation constraints,the obtained control performance can preserve good robustness.Global stability is also proven by mathematical derivations and verified by numerical simulations. | XU Yang LUO DeLin DUAN HaiBin | 2021 | Science China(Technological Sciences)2021，64，8： | 2 |
| 11 | Synthesis and Anti-HIV Activity of a Series of 6-Modified 2',3'-Dideoxyguanosine and 2',3'-Didehydro-2',3'- dideoxyguanosine Analogs显示文摘寻找潜在的 2,3-dideoxyguanosine (ddG ) 和 2,3-didehydro-2,3-dideoxyguanosine (D4G ) prodrugs,一系列 6-modified ddG, D4G 类似物在基于房间的试金为他们的 anti-HIV 活动和 cytotoxities 被综合并且评估。所有类似物显示出低 cytotoxicities,他们中的一些显示了良性的 anti-HIV 活动。在 vivo, ddGTP 和 D4TTP 的活跃 triphosphate 形式,被一个新奇、灵巧的“一个壶”也综合方法。由 Taq, Therminater DNA 聚合酶和在 DNA/RNA 海滨合并的 HIV 反向的 transcriptase (RT ) 的 ddGTP 和 D4TTP 的识别被一个非放射性方法调查, Km 是坚定的。 | Lujia Xie Xiantao Yang Delin Pan Yingli Cao Mou Cao Guichun Lin Zhu Guan Ying Guo Lihe Zhang Zhenjun Yang | 2013 | Chinese Journal of Chemistry2013，31，9： | 2 |
| 12 | Analysis of Spatial Clustering Optimization显示文摘Spatial clustering is widely used in many fields such as WSN (Wireless Sensor Networks), web clustering, remote sensing and so on for discovery groups and to identify interesting distributions in the underlying database. By discussing the relationships between the optimal clustering and the initial seeds, a clustering validity index and the principle of seeking initial seeds were proposed, and on this principle we recommend an initial seed-seeking strategy: SSPG (Single-Shortest-Path Graph). With SSPG strategy used in clustering algorithms, we find that the result of clustering is optimized with more probability. At the end of the paper, according to the combinational theory of optimization, a method is proposed to obtain optimal reference k value of cluster number, and is proven to be efficient. | YANG Jianfeng YAN Puliu XIA Delin GENG Qing | 2008 | Geo-Spatial Information Science2008，11，4： | 2 |
| 13 | GRAIN REFINEMENT IN BULK UNDERCOOLED Ni-BASED ALLOY显示文摘Fluxing of 5 g bulk melt Ni77Si13B10 permits high undercoolings to be attained prior to nu-cleation onset.Investigations of grain refinement in the bulk undercooled alloy as a function ofundercooling,recalescence behavior and cooling rate have been reported.A significant inhomo-geneity of reduction in grain size of a bulk sample is observed,which is caused by the different so-lidification conditions:(1)recalescence process,and(2)the followed plateau in which the heatrelease and extraction rates are equal.It is concluded that the homogeneous refined microstructurecan be achieved if the initial undercooling prior to nucleation,or cooling rate after recalescence isfurther increased. | Li,Delin Yang,Gencang Zhou,Yaohe Northwestern Polytechnical University,Xi’an 710072,China | 1993 | 中国有色金属学会会刊:英文版1993，3，4： | 2 |
| 14 | Secondary donor-derived CD19 CAR-T therapy is safe and efficacious in acute lymphoblastic leukemia with extramedullary relapse after first autologous CAR-T therapy显示文摘Despite the advancement of treatments,adults with relapsed/refractory(R/R)B-lineage acute lymphoblastic leukemia(B-ALL)have poor prognosis,with an expected five-year overall survival(OS)rate of 10%‒20%(Nguyen et al.,2008;Oriol et al.,2010).Extramedullary relapse of B-ALL is regarded as a highrisk factor generally associated with poor survival,occurring in about 15%to 20%of all relapsed patients(Ding et al.,2017;Sun et al.,2018).The central nervous system(CNS)and the testes are the most common sites of extramedullary relapse of B-ALL.In addition,extramedullary leukemia can appear in the skin,eyes,breasts,bones,muscles,and abdominal organs.The prognosis of relapsed extramedullary B-ALL after allogeneic hematopoietic stem cell transplantation(allo-HSCT)is extremely poor(Spyridonidis et al.,2012;Dahlberg et al.,2019).Conventional chemotherapy or radiation is often ineffective in such patients.At present,there are no optimal treatment strategies for treating extramedullary leukemia after allo-HSCT. | Delin KONG Tingting YANG Jia GENG Ruirui JING Qiqi ZHANG GuoqingWEI He HUANG Yongxian HU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，10： | 2 |
| 15 | Molecular simulations of charged complex fluids: A review显示文摘Molecular simulation plays an increasingly important role in studying the properties of complex fluid systems containing charges,such as ions,piezoelectric materials,ionic liquids,ionic surfactants,polyelectrolytes,zwitterionic materials,nucleic acids,proteins,biomembranes and etc.,where the electrostatic interactions are of special significance.Several methods have been available for treating the electrostatic interactions in explicit and implicit solvent models.Accurate and efficient treatment of such interactions has therefore always been one of the most challenging issues in classical molecular dynamics simulations due to their inhomogeneity and long-range characteristics.Currently,two major challenges remain in the application field of electrostatic interactions in molecular simulations;(i)improving the representation of electrostatic interactions while reducing the computational costs in molecular simulations;(ii)revealing the role of electrostatic interactions in regulating the specific properties of complex fluids.In this review,the calculation methods of electrostatic interactions,including basic principles,applicable conditions,advantages and disadvantages are summarized and compared.Subsequently,the specific role of electrostatic interactions in governing the properties and behaviors of different complex fluids is emphasized and explained.Finally,challenges and perspective on the computational study of charged systems are given. | Zhiyong Xu Zhongjin He Xuebo Quan Delin Sun Zhaohong Miao Hai Yu Shengjiang Yang Zheng Chen Jinxiang Zeng Jian Zhou | 2021 | Chinese Journal of Chemical Engineering2021，34，3： | 2 |
| 16 | Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress显示文摘(Rce1 ) 地岬和 a-factor-converting 酶 1 位于 endoplasmic 蜂窝胃(嗯) 并且被认为为绝大多数 CAAX 蛋白质处理的 endoproteolytic 负责。Endoplasmic 蜂窝胃应力(ERS ) 在肾的房间癌(RCC ) 起一个重要作用;然而,在 RCC 的 Rce1 的表示和角色广泛地没被学习。我们试图在 RCC 786-O 房间在嗯导致 apoptosis 在 RCC 纸巾和它的分子的机制调查 Rce1 的表示。我们首先使用了检测在肾的癌纸巾和 paracancerous 纸巾的 Rce1 表示的西方的弄污,量的反向的 transcriptase 聚合酶链反应,和 immunohistochemistry。Rce1 表示是在 RCC 的 upregulated,这被发现纸巾,和它的积极表示水平强烈与 clinicopathologic 特征被联系。下次,我们在人的胚胎的肾房间线 HEK293 和人的肾的癌房间线 786-O 检测了 Rce1 的表示, ACHN,和 A498。Rce1 的更高的表示在人的肾的癌房间线被发现,特别在 786-O 房间。在 786-O 房间的 Rce1 击倒增加的 apoptosis | Jianjun Li Delin Wang Junnan Liu Yunlang Qin Liangliang Huang Qiangfeng Zeng Maolin Xiao Jie Hu Qixin Yang Jiang He Li Mai Ying Li Wujiang Liu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 2 |
| 17 | Metabolic engineering of a methyltransferase for production of drug precursors demecycline and demeclocycline in Streptomyces aureofaciens显示文摘Demecycline(DMTC)and demeclocycline(DMCTC)are C6-demethylated derivatives of tetracycline(TC)and chlortetracycline(CTC),respectively.They are precursors of minocycline and tigecycline,which showed remarkable bioactivity against TC-resistant bacteria and have been used clinically for decades.In order to biosynthesize drug precursors DMTC and DMCTC,the function of a possible C-methyltransferase encoding gene ctcK was studied systematically in the CTC high-yielding industrial strain Streptomyces aureofaciens F3.TheΔctcK mutant accumulated two new products,which were turned out to be DMTC and DMCTC.Meanwhile,timecourse analysis of the fermentation products detected the epimers of DMTC and DMCTC transformed spontaneously.Finally,an engineering strain with higher productivity of DMCTC was constructed by deleting ctcK and overexpressing ctcP of three extra copies simultaneously.Construction of these two engineering strains not only served as a successful example of synthesizing required products through metabolic engineering,but also provided original strains for following elaborate engineering to synthesize more effective tetracycline derivatives. | Weinan Yang Lingxin Kong Qing Wang Zixin Deng Delin You | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 2 |
| 18 | Dynamic affine formation control of networked under-actuated quad-rotor UAVs with three-dimensional patterns显示文摘This paper focuses on the solution to the dynamic affine formation control problem for multiple networked underactuated quad-rotor unmanned aerial vehicles(UAVs)to achieve a configuration that preserves collinearity and ratios of distances for a target configuration.In particular,it is investigated that the quad-rotor UAVs are steered to track a reference linear velocity while maintaining a desired three-dimensional target formation.Firstly,by integrating the properties of the affine transformation and the stress matrix,the design of the target formation is convenient and applicable for various three-dimensional geometric patterns.Secondly,a distributed control method is proposed under a hierarchical framework.By introducing an intermediary control input for each quad-rotor UAV in the position loop,the necessary thrust input and the desired attitude are extracted.In the attitude loop,the desired attitude represented by the unit quaternion is tracked by the designed torque input.Both conditions of linear velocity unavailability and mutual collision avoidance are also tackled.In terms of Lyapunov theory,it is prooved that the overall closed-loop error system is asymptotically stable.Finally,two illustrative examples are simulated to validate the effectiveness of the proposed theoretical results. | XU Yang ZHENG Weiming LUO Delin DUAN Haibin | 2022 | Journal of Systems Engineering and Electronics2022，33，6： | 1 |
| 19 | Experimental study on the oxidation of MgB2 in air at high temperature显示文摘 | Yang Delin Sun Hongwei Liu Hongxia | 2003 | Supercond Sci Technol2003，16，： | 1 |
| 20 | Patent Propensity in Small Technologybased Firms:Evidence from Zhongguancun Science Park显示文摘用在科学在中国北京停的 Zhongguancun 管理的调查,这篇论文在小基于技术的公司的专利倾向上调查 R&D 人员相关的知识产权管理惯例的影响。R&D 人员相关的管理练习,这被发现,包括训练和报酬机制,在提高一个公司的心甘情愿获专利是有效的。特别地,我们发现报酬机制能否定地在一个公司的心甘情愿上节制尺寸的效果获专利。从分析出现了的一个含意是一个小公司能在由介绍开发得好的报酬机制获专利抵抗它的尺寸劣势。 | Delin Yang Xibao Li Jiagui Chen | 2010 | China & World Economy2010，18，1： | 1 |
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