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| 1 | Development of Human in vitro Brain-blood Barrier Model from Induced Pluripotent Stem Cell-derived Endothelial Cells to Predict the in vivo Permeability of Drugs显示文摘An in vitro blood-brain barrier(BBB) model is critical for enabling rapid screening of the BBB permeability of the drugs targeting on the central nervous system.Though many models have been developed, their reproducibility and renewability remain a challenge. Furthermore, drug transport data from many of the models do not correlate well with the data for in vivo BBB drug transport.Induced-pluripotent stem cell(i PSC) technology provides reproducible cell resources for in vitro BBB modeling.Here, we generated a human in vitro BBB model by differentiating the human i PSC(hi PSC) line GM25256 into brain endothelial-type cells. The model displayed BBB characteristics including tight junction proteins(ZO-1,claudin-5, and occludin) and endothelial markers(von Willebrand factor and Ulex), as well as high transendothelial electrical resistance(TEER)(1560 X.cm2±230 X.cm2) and c-GTPase activity. Co-culture with primary rat astrocytes significantly increased the TEER of the model(2970 X.cm2 to 4185 X.cm2). RNAseq analysis confirmed the expression of key BBB-related genes in the hi PSC-derived endothelial cells in comparison with primary human brain microvascular endothelial cells,including P-glycoprotein(Pgp) and breast cancer resistant protein(BCRP). Drug transport assays for nine CNS compounds showed that the permeability of non-Pgp/BCRP and Pgp/BCRP substrates across the model was strongly correlated with rodent in situ brain perfusion data for these compounds(R2= 0.982 and R2= 0.9973,respectively), demonstrating the functionality of the drug transporters in the model. Thus, this model may be used to rapidly screen CNS compounds, to predict the in vivo BBB permeability of these compounds and to study the biology of the BBB. | Yuan Li Xueying Sun Houfu Liu Liang Huang Guofeng Meng Yu Ding Wenji Su Jiaqi Lu Sophie Gong Georg C.Terstappen Ru Zhang Wandong Zhang | 2019 | Neuroscience Bulletin2019，35，6： | 3 |
| 2 | High-tempera-ture liquid-liquid structure transition in liquid Sn-Bi alloys:experimental evidence by electrical resistivity method显示文摘 | Li Xianfen Zu Fangqiu Ding Houfu | 2006 | Physics Letters A2006，354，4： | 1 |
| 3 | Influence of Impact Energy on Impact Corrosion-abrasion of High Manganese Steel显示文摘 | Xiaodong Du Houfu Ding Kai | 2007 | Journal of Wuhan University of Technology2007，22，3： | 1 |
| 4 | High-temperature liquid-liquid structure transition in liquid Sn-Bi alloys: expefimental evidence by electrical resistively method 显示文摘 | Li Xianfen Zu Fangqiu Ding Houfu | 2006 | Physics Letters A2006，354，4： | 1 |
| 5 | Effect of component and microstrueture on impact wear property and mechanism of steels in corrosive condition 显示文摘 | DING Houfu CUI Fangming DU Xiaodong | 2006 | Materials Science and Engineering: A2006，421，12： | 1 |
| 6 | A study of the solid state reaction betweensilicon carbide and iron显示文摘 | TANG Wenming ZHENG Zhixiang DING Houfu | 2002 | Mater Chem Phys2002，74，： | 1 |
| 7 | Study on Microstructure and Thermal Stability Behavior of Rapidly Solidified Al-Fe-Ce Alloy显示文摘Rapidly solidified Al 8Fe 4Ce alloy was prepared by melt spinning.As quenched and as annealed microstructures were studied by TEM and energy dispersive spectrum analysis.The microhardness of the alloy at different annealing temperature was measured.The results obtained indicated that as quenched microstructure varied with different cooling rates.The microstructure annealed at 300℃ was much the same as that of the as quenched.The dispersed phases at grain boundary of the microstructure annealed at 400℃ became coarsening.After annealing at 450℃ for 2 hours,the primary phase and the intercellular dispersed phases,metastable phase Al 6Fe and Al 20 Fe 5Ce respectively,coarsened further.The soften temperature was deduced at over 300℃ by measuring microhardness. | Chen Yiqing,Su Yong,Ding Houfu and Zheng Yuchun (Department of Materials Science & Technology,Hefei University of Technology,Hefei 230009) | 2000 | Rare Metals2000，19，3： | 0 |
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