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| 1 | Expression of MMIF, HIF-1α and VEGF in Serum and Endometrial Tissues of Patients with Endometriosis显示文摘 | Fan ZHANG Xiao-ling LIU Wan WANG Hong-ling DONG Yu-fang XIA Li-ping RUAN Li-ping LIU | 2018 | Current Medical Science2018，38，3： | 23 |
| 2 | Fecal microbiota transplantation induces remission of infantile allergic colitis through gut microbiota re-establishment显示文摘AIM To investigate the impact of fecal microbiota transplantation(FMT) treatment on allergic colitis(AC) and gut microbiota(GM).METHODS We selected a total of 19 AC infants,who suffered from severe diarrhea/hematochezia,did not relieve completely after routine therapy or cannot adhere to the therapy,and were free from organ congenital malformations and other contraindications for FMT. Qualified donor-derived stools were collected and injected to the AC infants via a rectal tube. Clinical outcomes and follow-up observations were noted. Stools were collected from ten AC infants before and after FMT,and GM composition was assessed for infants and donors using 16 S r DNA sequencing analysis.RESULTS After FMT treatment,AC symptoms in 17 infants were relieved within 2 d,and no relapse was observed in the next 15 mo. Clinical improvement was also detected in the other two AC infants who were lost to followup. During follow-up,one AC infant suffered from mild eczema and recovered shortly after hormone therapy. Based on the 16 S r DNA analysis in ten AC infants,most of them(n = 6) had greater GM diversity after FMT. As a result,Proteobacteria decreased(n = 6) and Firmicutes increased(n = 10) in post-FMT AC infants. Moreover,Firmicutes accounted for the greatest proportion of GM in the patients. At the genus level,Bacteroides(n = 6),Escherichia(n = 8),and Lactobacillus(n = 4) were enriched in some AC infants after FMT treatment,but the relative abundances of Clostridium(n = 5),Veillonella(n = 7),Streptococcus(n = 6),and Klebsiella(n = 8) decreased dramatically.CONCLUSION FMT is a safe and effective method for treating pediatric patients with AC and restoring GM balance. | Sheng-Xuan Liu Yin-Hu Li Wen-Kui Dai Xue-Song Li Chuang-Zhao Qiu Meng-Ling Ruan Biao Zou Chen Dong Yan-Hong Liu Jia-Yi He Zhi-Hua Huang Sai-Nan Shu | 2017 | World Journal of Gastroenterology2017，23，48： | 19 |
| 3 | Endoplasmic reticulum stress is involved in podocyte apoptosis induced by saturated fatty acid palmitate显示文摘 | TAO Jian-ling WEN Yu-bing SHI Bing-yang ZHANG Hong RUAN Xiong-zhong LI Hang LI Xue-mei DONG Wen-ji LI Xue-wang | 2012 | Chinese Medical Journal2012，，17： | 15 |
| 4 | Changing Proportions of HIV-1 Subtypes and Transmitted Drug Resistance Among Newly Diagnosed HIV/AIDS Individuals—China,2015 and 2018显示文摘Introduction:With the expansion of human immunodeficiency virus(HIV)antiretroviral therapy(ART),HIV drug resistance is becoming more and more serious.This study describes the changing prevalence of HIV-1 subtypes and transmitted drug resistance(TDR)among newly diagnosed individuals in China,2015 and 2018.Methods:A total of 8,980 individuals in 2015 and 2018 from 31 provincial-level administrative divisions(PLADs)were enrolled in this study.Viral RNAs were amplified and sequenced using an in-house polymerase chain reaction(PCR)protocol.The Stanford HIV Drug Resistance Database(HIVdb)was used to predict susceptibility to 12 antiretroviral drugs.Results:The prevalence of TDR was not significantly increased over time.The prevalence of TDR was 3.8%and 4.4%in 2015 and 2018,respectively(P=0.13).The prevalence of CRF55_01B increased from 2.3%in 2015 to 3.9%in 2018(P<0.001).The drug resistance prevalence of nonnucleoside reverse transcriptase inhibitors(NNRTI)increased from 2.4%in 2015 to 3.3%in 2018(P<0.01).The prevalence of E138(P<0.001),H221(P=0.03),and V179(P<0.001)mutations increased from 0.30%,0.09%,and 0.70%in 2015 to 1.10%,0.30%,and 1.70%in 2018,respectively.Conclusions:HIV drug resistance affects the effect of antiretroviral treatment,so the monitoring of HIV TDR should be strengthened to control the transmission of HIV drug resistance. | Jingjing Hao Shan Zheng Mengze Gan Aobo Dong Ruihua Kang Miaomiao Li Shuai Zhao Jing Hu Chang Song Lingjie Liao Yi Feng Yiming Shao Yuhua Ruan Hui Xing | 2021 | China CDC weekly2021，3，53： | 13 |
| 5 | Science Letters:IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1显示文摘Insulin-like growth factor binding-protein-7 (IGFBP7) was obtained from our previous colonic adenocarcinoma (CRC) and normal mucosa suppression subtraction hybridization (SSH) cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR (MSP) and bisulfite sodium PCR (BSP), we found that its expression was associated with DNA hypomethylation of exon1. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2’-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the func-tional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony for-mation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colo-rectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1. | RUAN Wen-jing LIN Jie XU En-ping XU Fang-ying MA Yu DENG Hong HUANG Qiong LV Bing-jian HU Hu CUI Jing DI Mei-juan DONG Jian-kang LAI Mao-de | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，11： | 11 |
| 6 | Changes of main secondary metabolites in leaves of Ginkgo biloba in response to ozone fumigation显示文摘To investigate the effect of elevated O3 on the accumulation of main secondary metabolites in leaves of Ginkgo biloba L.,four-year-old trees were exposed in open-top chambers with ambient air and the air with twice ambient O3 concentration in Shenyang in 2006.Elevated O3 increased the concentrations of terpenes,but decreased the concentrations of phenolics in G.biloba leaves.The results showed that secondary compounds from G.biloba leaves responded to the elevated O3 exposure in a different way when compared to previous studies which showed elevated O3 increased the concentrations of phenolics but had no effect on the terpenes in leaves of other deciduous trees.Furthermore,reduced synthesis of phenolics may decrease the resistance of G.biloba to O3 and other environmental factors.On the other hand,the induced synthesis of terpenes may enhance the antioxidant abilities in G.biloba leaves at the end of O3 fumigation. | HE Xingyuan HUANG Wei CHEN Wei DONG Tian LIU Changbing CHEN Zhenju XU Sheng RUAN Yanan | 2009 | Journal of Environmental Sciences2009，21，2： | 8 |
| 7 | Development of nutritious rice with high zinc/selenium and low cadmium in grains through QTL pyramiding显示文摘Enriching zinc(Zn) and selenium(Se) levels,while reducing cadmium(Cd) concentration in rice grains is of great benefit for human diet and health.Large natural variations in grain Zn, Se, and Cd concentrations in different rice accessions enable Zn/Sebiofortification and Cd-minimization through molecular breeding. Here, we report the development of new elite varieties by pyramiding major quantitative trait loci(QTLs) that significantly contribute to high Zn/Se and low Cd accumulation in grains. A chromosome segment substitution line CSSLGCC7 with the PA64s-derived GCC7 allele in the 93-11 background, exhibited steadily higher Mn and lower Cd concentrations in grains than those of 93-11. This elite chromosome segment substitution line(CSSL) was used as the core breeding material to cross with CSSLs harboring other major QTLs for essential mineral elements, especially CSSLGZC6 for grain Zn concentration and CSSLGSC5 for grain Se concentration. The CSSLGCC7+GZC6 and CSSLGCC7+GSC5 exhibited lower Cd concentration with higher Zn and Se concentrations in grains, respectively. Our study thus provides elite materials for rice breeding targeting high Zn/Se and low Cd concentrations in grains. | Chaolei Liu Shilin Ding Anpeng Zhang Kai Hong Hongzhen Jiang Shenglong Yang Banpu Ruan Bin Zhang Guojun Dong Longbiao Guo Dali Zeng Qian Qian Zhenyu Gao | 2020 | Journal of Integrative Plant Biology2020，62，3： | 7 |
| 8 | Influences of solid particles on the formation of the third phase crud during solvent extraction显示文摘The influences of solid particles in leach solution on the formation of the third phase crud during solvent extrac-tion of copper were studied. Analyzed from the point of view of pH value and surface tension, the study results showed that the solid particle is one of the most important contributors for the formation of the third phase crud. During solvent extrac-tion, if the pH value was greater than 2.30, the number of solid particles in the mother solution increased, in which case the possibility of forming the third phase crud could also increase, and the interface tension value might grow in pace with the quantity of the third phase crud. | ZHENG Qi RUAN Renman WEN Jiankang SONG Yongsheng DONG Qinghai WU Minglin YAO Guocheng | 2007 | Rare Metals2007，26，1： | 6 |
| 9 | Mechanism for calcite dissolution and its contribution to development of reservoir porosity and permeability in the Kela 2 gas field,Tarim Basin,China显示文摘This study is undertaken to understand how calcite precipitation and dissolution contributes to depth-related changes in porosity and permeability of gas-bearing sandstone reservoirs in the Kela 2 gas field of the Tarim Basin, Northwestern China. Sandstone samples and pore water samples are col-lected from well KL201 in the Tarim Basin. Vertical profiles of porosity, permeability, pore water chem-istry, and the relative volume abundance of calcite/dolomite are constructed from 3600 to 4000 m below the ground surface within major oil and gas reservoir rocks. Porosity and permeability values are in-versely correlated with the calcite abundance, indicating that calcite dissolution and precipitation may be controlling porosity and permeability of the reservoir rocks. Pore water chemistry exhibits a sys-tematic variation from the Na2SO4 type at the shallow depth (3600-3630 m), to the NaHCO3 type at the intermediate depth (3630―3695 m),and to the CaCl2 type at the greater depth (3728―3938 m). The geochemical factors that control the calcite solubility include pH, temperature, pressure, Ca2+ concen-tration, the total inorganic carbon concentration (ΣCO2), and the type of pore water. Thermodynamic phase equilibrium and mass conservation laws are applied to calculate the calcite saturation state as a function of a few key parameters. The model calculation illustrates that the calcite solubility is strongly dependent on the chemical composition of pore water, mainly the concentration difference between the total dissolved inorganic carbon and dissolved calcium concentration (i.e., [ΣCO2] -[Ca2+]). In the Na2SO4 water at the shallow depth, this index is close to 0, pore water is near the calcite solubility. Calcite does not dissolve or precipitate in significant quantities. In the NaHCO3 water at the intermedi-ate depth, this index is greater than 0, and pore water is supersaturated with respect to calcite. Massive calcite precipitation was observed at this depth interval and this intensive cementation is responsible for decreased porosity and permeability. In the CaCl2 water at the greater depth, pore water is un-der-saturated with respect to calcite, resulting in dissolution of calcite cements, as consistent with microscopic dissolution features of the samples from this depth interval. Calcite dissolution results in formation of high secondary porosity and permeability, and is responsible for the superior quality of the reservoir rocks at this depth interval. These results illustrate the importance of pore water chemis-try in controlling carbonate precipitation/dissolution, which in turn controls porosity and permeability of oil and gas reservoir rocks in major sedimentary basins. | YU BingSong1,3, DONG HailLiang2,3 & RUAN Zhuang1,3 1 State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Beijing 100083, China 2 Department of Geology, Miami University,Oxford, OH 45056, USA 3 Geomicrobiology Laboratory, China University of Geosciences, Beijing, 100083, China | 2008 | Science China Earth Sciences2008，51，4： | 6 |
| 10 | Experimental free-space quantum secure direct communication and its security analysis显示文摘We report an experimental implementation of free-space quantum secure direct communication based on single photons.The quantum communication scheme uses phase encoding,and the asymmetric Mach–Zehnder interferometer is optimized so as to automatically compensate phase drift of the photons during their transitions over the free-space medium.At a 16 MHz pulse repetition frequency,an information transmission rate of 500 bps over a 10 m free space with a mean quantum bit error rate of 0.49%±0.27%is achieved.The security is analyzed under the scenario that Eve performs the collective attack for single-photon state and the photon number splitting attack for multi-photon state in the depolarizing channel.Our results show that quantum secure direct communication is feasible in free space. | DONG PAN ZAISHENG LIN JIAWEI WU HAORAN ZHANG ZHEN SUN DONG RUAN LIUGUO YIN AND GUI LU LONG | 2020 | Photonics Research2020，8，9： | 5 |
| 11 | Time correction of the ocean bottom seismometers deployed at the southwest Indian ridge using ambient noise cross-correlation显示文摘Seismic monitoring using ocean bottom seismometers(OBS) is an efficient method for investigating earthquakes in mid-ocean ridge far away from land. Clock synchronization among the OBSs is difficult without direct communication because electromagnetic signals cannot propagate efficiently in water. Time correction can be estimated through global positioning system(GPS) synchronization if clock drift is linear before and after the deployment. However, some OBSs in the experiments at the southwest Indian ridge(SWIR) on the Chinese DY125-34 cruise had not been re-synchronized from GPS after recovery. So we attempted to estimate clock drift between each station pairs using time symmetry analysis(TSA) based on ambient noise cross-correlation. We tested the feasibility of the TSA method by analyzing daily noise cross-correlation functions(NCFs) that extract from the data of another OBS experiment on the Chinese DY125-40 cruise with known clock drift and the same deployment site. The results suggest that the NCFs' travel time of surface wave between any two stations are symmetrical and have an opposite growing direction with the date. The influence of different band-pass filters,different components and different normalized methods was discussed. The TSA method appeared to be optimal for the hydrophone data within the period band of 2–5 s in dozens of km-scale interstation distances. A significant clock drift of ~2 s was estimated between OBSs sets through linear regression during a 108-d deployment on the Chinese cruise DY125-34. Time correction of the OBS by the ambient noise cross-correlation was demonstrated as a practical approach with the appropriate parameters in case of no GPS re-synchronization. | LIU Yunlong LIU Cai TAO Chunhui YAO Huajian QIU Lei WANG Ao RUAN Aiguo WANG Hanchuang ZHOU Jianping LI Huaiming DONG Chuanwan | 2018 | Acta Oceanologica Sinica2018，37，5： | 5 |
| 12 | Modern power systems control centers:From EMS to AEMS显示文摘Traditional energy management system (EMS) plays an indispensable role in control centers of electric power systems. However, it also has several shortcomings, including lack of real-time closed-loop control, unreliable functional modules, and difficult to exchange data. The electric hybrid control theory (EHCT) has been proposed as an innovative and effective means to overcome these shortcomings. This paper argues that the EHCT provides a theoretical foundation for the advanced energy management system (AEMS) that can achieve multi-objective, near-optimal, and closed-loop control of electric power grids. This paper also discusses the significance for control centers to evolve from the traditional EMS to the AEMS. Furthermore, this paper points out that the traditional EMS can be considered as an integral part of the AEMS and that the AEMS can be built upon the traditional EMS. Thus, the resources that are currently available can be fully utilized to achieve near-optimal and closed-loop control of power system operations. | HE GuangYu SUN YingYun RUAN QianTu WANG Wei DONG ShuFeng | 2009 | Science China(Technological Sciences)2009，52，2： | 5 |
| 13 | Photonic matrix multiplication lights up photonicaccelerator and beyond显示文摘Matrix computation,as a fundamental building block of information processing in science and technology,contributes most of the computational overheads in modern signal processing and artificial intelligence algorithms.Photonic accelerators are designed to accelerate specific categories of computing in the optical domain,especially matrix multiplication,to address the growing demand for computing resources and capacity.Photonic matrix multiplication has much potential to expand the domain of telecommunication,and artificial intelligence benefiting from its superior performance.Recent research in photonic matrix multiplication has flourished and may provide opportunities to develop applications that are unachievable at present by conventional electronic processors.In this review,we first introduce the methods of photonic matrix multiplication,mainly including the plane light conversion method,Mach–Zehnder interferometer method and wavelength division multiplexing method.We also summarize the developmental milestones of photonic matrix multiplication and the related applications.Then,we review their detailed advances in applications to optical signal processing and artificial neural networks in recent years.Finally,we comment on the challenges and perspectives of photonic matrix multiplication and photonic acceleration. | Hailong Zhou Jianji Dong Junwei Cheng Wenchan Dong Chaoran Huang Yichen Shen Qiming Zhang Min Gu Chao Qian Hongsheng Chen Zhichao Ruan Xinliang Zhang | 2022 | Light(Science & Applications)2022，11，2： | 4 |
| 14 | A new alkaloid from Lycoris aurea显示文摘新碱从 Lycoris 的灯泡被孤立脲(L’ Herit ) 植物。它的结构包括 2D NMR 通过化学、分光镜的研究作为 3-o-ethyltazettinol 被建立。 | Hui Fang Pi Peng Zhang Han Li Ruan Yong Hui Zhang Han Dong Sun Ji Zhou Wu | 2009 | Chinese Chemical Letters2009，20，11： | 4 |
| 15 | Brillouin cavity optomechanics sensing with enhanced dynamical backaction显示文摘Based on the dispersive interaction between a high quality factor microcavity and nano-objects,whisperinggallery-mode microcavities have been widely used in highly sensitive sensing.Here,we propose a novel method to enhance the sensitivity of the optical frequency shift and reduce the impact of the laser frequency noise on the detection resolution through Brillouin cavity optomechanics in a parity-time symmetric system.The optical spring effect is sensitive to the perturbation of optical modes around the exceptional point.By monitoring the shift of the mechanical frequency,the detection sensitivity for the optical frequency shift is enhanced by 2 orders of magnitude compared with conventional approaches.We find the optical spring effect is robust to the laser frequency noise around the exceptional point,which can reduce the detection limitation caused by the laser frequency instability.Thus,our method can improve the sensing ability for nano-object sensing and other techniques based on the frequency shift of the optical mode. | GUO-QING QIN MIN WANG JING-WEI WEN DONG RUAN GUI-LU LONG | 2019 | Photonics Research2019，7，12： | 4 |
| 16 | Depression compromises antiviral innate immunity via the AVP-AHI1-Tyk2 axis显示文摘Depression is a serious public-health issue.Recent reports have suggested higher susceptibility to viral infections in depressive patients.However,how depression affects antiviral innate immune signaling remains unknown.Here,we revealed a reduction in expression of Abelson helper integration site 1(AHI1)in the peripheral blood mononuclear cells(PBMCs)and macrophages from the patients with major depressive disorder(MDD),which leads to attenuated antiviral immune response.We found that depression-related arginine vasopressin(AVP)induces reduction of AHI1 in macrophages.Further studies demonstrated that AHI1 is a critical stabilizer of basal type-I-interferon(IFN-I)signaling.Mechanistically,AHI1 recruits OTUD1 to deubiquitinate and stabilize Tyk2,while AHI1 reduction downregulates Tyk2 and IFN-I signaling activity in macrophages from both MDD patients and depression model mice.Interestingly,we identified a clinical analgesic meptazinol that effectively stimulates AHI1 expression,thus enhancing IFN-I antiviral defense in depression model mice.Our study promotes the understanding of the signaling mechanisms of depression-mediated antiviral immune dysfunction,and reveals meptazinol as an enhancer of antiviral innate immunity in depressive patients. | Hong-Guang Zhang Bin Wang Yong Yang Xuan Liu Junjie Wang Ning Xin Shifeng Li Ying Miao Qiuyu Wu Tingting Guo Yukang Yuan Yibo Zuo Xiangjie Chen Tengfei Ren Chunsheng Dong Jun Wang Hang Ruan Miao Sun Xingshun Xu Hui Zheng | 2022 | Cell Research2022，32，10： | 3 |
| 17 | Nkx2-1:a novel tumor biomarker of lung cancer显示文摘Nkx2-1 (Nkx homeobox-1 gene), also known as TTF-1 (thyroid transcription factor-1), is a tissue-specific transcription factor of the thyroid, lung, and ventral forebrain. While it has been shown to play a critical role in lung development and lung cancer differentiation and morphogenesis, molecular mechanisms mediating Nkx2-1 cell- and tissue-specific expression in normal and cancerous lungs have yet to be fully elucidated. The recent identification of prognostic biomarkers in lung cancer, particularly in lung adenocarcinoma (ADC), and the different reactivity of patients to chemotherapeutic drugs have opened new avenues for evaluating patient survival and the development of novel effective therapeutic strategies. The function of Nkx2-1 as a proto-oncogene was recently characterized and the gene is implicated as a contributory factor in lung cancer development. In this review, we summarize the role of this transcription factor in the development, diagnosis, and prognosis of lung cancer in the hope of providing insights into the utility of Nkx2-1 as a novel biomarker of lung cancer. | LiYANG Min LIN Wen-jing RUAN Liang-liang DONG En-guo CHEN Xiao-hong WU Ke-jing YING | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，11： | 3 |
| 18 | A Diagnostic Tool for Identification of Etiologies of Fever of Unknown Origin in Adult Patients显示文摘The diagnosis and treatment of fever of unknown origin (FUO) are huge challenges to clinicians.Separating the etiologies of FUO into infectious and non-infectious disease is conducive to clinical physicians not only on making decisions rapidly concerning the prescription of suitable antibiotics but also on further analysis of the final diagnosis.In order to develop and validate a diagnostic tool to efficiently distinguish the etiologies of adult FUO patients as infectious or non-infectious disease,FUO patients from the departments of infectious disease and internal medicine in three Chinese tertiary hospitals were enrolled retrospectively and prospectively.By using polynomial logistic regression analysis,the diagnostic formula and the associated scoring system were developed.The variables included in this diagnostic formula were from clinical evaluations and common laboratory examinations.The proposed tool could discriminate infectious and noninfectious causes of FUO with an area under receiver operating characteristic curve (AUC) of 0.83,sensitivity of 0.80 and specificity of 0.75.This diagnosis tool could predict the infectious and non-infectious causes of FUO in the validation cohort with an AUC of 0.79,sensitivity of 0.79 and specificity of 0.70.The results suggested that this diagnostic tool could be a reliable tool to discriminate between infectious and non-infectious causes of FUO. | Man-zhi ZHAO Qiu-rong RUAN Ming-you XING Sheng WEI Dong XU Zhu-hua WU Lin ZHU Ji-ling ZHU Cai-feng ZHENG Shi LIU Zu-jiang YU Jun-ying QI Jian-xin SONG | 2019 | Current Medical Science2019，39，4： | 3 |
| 19 | Nutritional requirements of meat-type and egg-type ducks: what do we know?显示文摘The demand for duck meat, duck eggs, and associated products is increasing each year. Classic and modern selection programs have been applied to enhance the economic traits of ducks to satisfy the requirements of consumers and enhance the incomes of producers. The nutritional requirements of unselected ducks may not be adequate, however, to fulfill the potential productivity performance of modern birds, including both meat-type and egg-type ducks. In particular, an imbalanced diet is associated with low productive performance and signs of nutritional deficiency(if insufficient nutrients are supplied), as well as with high feed costs and manure problems that reflect flock health and welfare(if excessive nutrients are supplied). Thus, the main aim of this review is to summarize the results of previous studies that estimated the nutrient requirements of meat-type and egg-type ducks in order to evaluate current knowledge and to identify further issues that need to be addressed. In addition,the results obtained in previous studies are compared in order to understand how to lower commercial feed costs,fulfill the genetic potential of selected ducks, protect the environment from pollution, and satisfy the welfare and health needs of ducks. | Ahmed Mohamed Fouad Dong Ruan Shuang Wang Wei Chen Weiguang Xia Chuntian Zheng | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 3 |
| 20 | Genetic Analysis and QTL Mapping of Mature Seed Culturability in Indica Rice显示文摘Genetic segregation analysis for mature seed culturability was conducted using recombinant inbred lines derived from a cross of indica rice,Yangdao 6 and Pei’ai 64s.Three indices of seed culturability,the frequency of callus induction,the frequency of brown callus and the increase of callus weight were investigated.A combined genetic map constructed with simple sequence repeat(SSR),sequence tag site(STS),cleaved amplified polymorphic sequences(CAPS)and single nucleotide polymorphism(SNP)markers covered a total distance of 1 732.5 cM,averaging approximately 12 cM between two neighboring loci.Three QTLs on chromosomes 7,7 and 10 were detected for the frequency of callus induction;three QTLs on chromosomes 6,7 and 9 were detected for the frequency of brown callus;and two QTLs on chromosomes 5 and 7 were detected for the increase of callus weight.Common QTLs mapped at the interval flanking RM5481 and RM6835 on chromosome 7 were identified to be involved in the frequency of callus induction and the frequency of brown callus,explaining 7.29%and 12.52%of phenotypic variation,respectively.A total of 14 epistatic effects were detected for the three indices of mature seed culturability. | TIAN Fu-kuan RUAN Ban-pu YAN Mei-xian YE Shi-fang PENG You-lin DONG Guo-jun ZHU Li HU Jiang YAN Hong-lan GUO Long-biao QIAN Qian GAO Zhen-yu | 2013 | Rice science2013，20，5： | 3 |
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