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| 1 | Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy显示文摘Conventional immunohistochemistry(IHC)is a widely used diagnostic technique in tissue pathology.However,this technique is associated with a number of limitations,including high inter-observer variability and the capacity to label only one marker per tissue section.This review details various highly multiplexed techniques that have emerged to circumvent these constraints,allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition,cellular functional and cell-cell interactions.Among these techniques,multiplex Immunohistochemistry/Immunofluorescence(mIHC/IF)has emerged to be particularly promising.mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible,efficient and cost-effective tissue studies.This technique has immediate potential for translational research and clinical practice,particularly in the era of cancer immunotherapy. | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2020 | Cancer Communications2020，40，4： | 11 |
| 2 | Ficolin A exacerbates severe H1N1 influenza virus infection-induced acute lung immunopathological injury via excessive complement activation显示文摘Severe pandemic influenza A(H1N1)2009 virus(pH1N1)infection causes significant morbidity and mortality.We and other groups have reported that immunopathological damage induced by excessive pulmonary inflammation plays a critical role in the pathogenesis of severe pneumonia and provides novel strategies for the treatment of severe influenza infection[1–3].The complement system plays critical roles in both innate and adaptive immunity by activating the classical,alternative and lectin pathways[4]. | Xu Wu Linlin Bao Ziqi Hu Duoduo Yao Fengdi Li Hui Li Xiaoxue Xu Yunqing An Xi Wang Bin Cao Xulong Zhang | 2021 | Cellular & Molecular Immunology2021，18，9： | 6 |
| 3 | 肿瘤免疫治疗时代的多重免疫组织化学/免疫荧光技术显示文摘常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单张切片上同时检测多个标志物,可对细胞组成、细胞功能和细胞间相互作用进行整合研究。在这些技术中,多重免疫组织化学/免疫荧光(multiplex immunohistochemistry/immunofluorescence,mIHC/IF)已成为最具应用前景的技术。mIHC/IF为开展高重复性、高效、高性价比的组织研究提供了高通量多重染色技术和标准化的定量分析。该技术在转化研究和临床实践中,尤其是肿瘤免疫治疗中极具应用价值。 | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2021 | 癌症2021，40，9： | 2 |
| 4 | China's Textile to Encounter Anti-dumping Peak in 2009显示文摘 According to the Paragraph 242of China's WTO Accession Report, by December 31,2008,the importers are still allowed to set limitations on the quantities of the products on which China has abandoned the quotas, basing on the condition that they recognize that the Chinese products imported have disordered their domestic markets. Up till now, the USA, the EU,South Korea, Japan, Canada, India, etc.,have launched legislation process in line with the Paragraph 242.…… | Zhang Qingyong Wu Shengkui Wen Duoduo | 2005 | China's Foreign Trade2005，，20： | 0 |
| 5 | Trends in the Prevalence of Births with Chromosomal Abnormalities—Haidian District,Beijing Municipality,China,2013–2022显示文摘Summary What is already known about this topic?The primary causes of early miscarriage and stillbirth are chromosomal abnormalities(CAs)whose prevalence has been observed to increase in recent years.What is added by this report?According to data received from a hospital-based birthdefect surveillance system in the Haidian District,Beijing,there was a significant increase in the prevalence of CAs along with most subtypes from 2013 to 2022. | Shuangbo Xia Chao Meng Xiao Cheng Duoduo Wang Yaxian Wu Zhiwen Li Yuan Wei Jufen Liu | 2023 | China CDC weekly2023，5，36： | 0 |
| 6 | Ganoderma atrum polysaccharide relieves mitochondrial dysfunction to alleviate hydrogen peroxide-induced senescence via activating autophagy显示文摘Polysaccharides from genus Ganoderma have prominent anti-aging effect,but their mechanisms are incompletely clarified.In our previous experiments,Ganoderma atrum polysaccharide(PSG)was exhibited to significantly alleviate senescence and DNA damage of A375 cells.To investigate its underlying mechanism,we conducted RNA-Seq analysis and found that PSG upregulated autophagy and mitochondria pathways compared to the model group.Further experiments showed that PSG relieved mitochondrial dysfunction via inhibiting the accumulation of reactive oxygen species(ROS)and loss of the mitochondrial membrane potential(MMP).Meanwhile,PSG activated autophagy and enhanced lysosomal function through transcription factor EB(TFEB).Interestingly,the pretreatment of autophagy inhibitors bafilomycin A1(Baf A1)reversed the ROS decline induced by PSG,which subsequently increased cellular senescence of A375.In addition,PSG had a similar anti-aging effect on normal fibroblast WI-38.Taken together,PSG might reduce ROS levels through activating autophagy,which in turn suppressed mitochondrial dysfunction and cellular senescence. | Mi Guo Ke Zhang Duoduo Zhang Yujia Zhou Linyuan Liu Yu Wu Xingtao Zhou Shaoping Nie | 2022 | Journal of Future Foods2022，2，3： | 0 |
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