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1Impact of meteorological conditions on a nine-day particulate matter pollution event observed in December 2013,Shanghai,China显示文摘A severe particulate matter pollution event occurred in Shanghai from 1 to 9 December 2013.The mean hourly mass concentrations of PM_(2.5) and PM_(10) were 211.9 and 249.0 μg/m^3,respectively.Reanalysis data,in situ,and remote-sensing measurements were used to examine the impacts of meteorological conditions on this event.It was found that the synoptic pattern of weak pressure,the reduced planetary boundary layer height,and the passage of two cold fronts were key factors causing the event.Four stages were identified during this event based on the evolution of its PM_(2.5) levels and weather conditions.The highest concentration of PM2.5(602 μg/m^3) was observed in stage 3.High PM2.5 concentrations were closely associated with a low local ventilation index,with an average of 505m^2/s,as well as with the influx of pollutants from upstream,transported by the cold fronts.Jianming Xu Fengxia Yan Ying Xie Fengyun Wang Jianbin Wu Qingyan Fu 2015Particuology2015,13,3:17
2Combining CLUE-S and SWAT Models to Forecast Land Use Change and Non-point Source Pollution Impact at a Watershed Scale in Liaoning Province, China显示文摘Non-point source(NPS) pollution has become a major source of water pollution. A combination of models would provide the necessary direction and approaches designed to control NPS pollution through land use planning. In this study, NPS pollution load was simulated in urban planning, historic trends and ecological protection land use scenarios based on the Conversion of Land Use and its Effect at Small regional extent(CLUE-S) and Soil and Water Assessment Tool(SWAT) models applied to Hunhe-Taizi River Watershed, Liaoning Province, China. Total nitrogen(TN) and total phosphorus(TP) were chosen as NPS pollution indices. The results of models validation showed that CLUE-S and SWAT models were suitable in the study area. NPS pollution mainly came from dry farmland, paddy, rural and urban areas. The spatial distribution of TN and TP exhibited the same trend in 57 sub-catchments. The TN and TP had the highest NPS pollution load in the western and central plains, which concentrated the urban area and farm land. The NPS pollution load would increase in the urban planning and historic trends scenarios, and would be even higher in the urban planning scenario. However, the NPS pollution load decreased in the ecological protection scenario. The differences observed in the three scenarios indicated that land use had a degree of impact on NPS pollution, which showed that scientific and ecologically sound construction could effectively reduce the NPS pollution load in a watershed. This study provides a scientific method for conducting NPS pollution research at the watershed scale, a scientific basis for non-point source pollution control, and a reference for related policy making.LIU Miao LI Chunlin HU Yuanman SUN Fengyun XU Yanyan CHEN Tan 2014Chinese Geographical Science2014,24,5:15
3Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryza rufipogon Griff.显示文摘Diversity and nitrogenase activity of endophytic diazotrophs colonized in the wild rice Oryza rufipogon Griff grown in Boluo, Huilai County in Guangdong Province and Lingshui County in Hainan Province were studied. Thirty-seven isolates obtained from Oryza rufipogon were identified as putative endophytic nitrogen-fixing bacteria by ARA (acetylene reduction assay) test and further confirmed by PCR amplification of nifH gene fragments. All obtained strains have ARA activity and the same sized nifH gene fragments. Above the similarity level of 80%, the obtained isolates were assigned as Group Ⅰ to Ⅷ by the clustering of IS-PCR fingerprints. The SDS-PAGE whole-cell protein patterns were similar to those of IS-PCR fingerprints. Components and contents of fatty acid methyl esters (FAMEs) were used to differentiate the representative strains (Ls13, Ls8, BL1, BL12, HL6, Ls4) from Group Ⅰ to Group Ⅵ. The six representative strains showed significant difference in contents and components of cellular fatty acid methyl ester. 16S rDNA sequencing analysis showed that strains of Group Ⅰ to Ⅶ were located in Enterobacteraceae (γ-proteobacteria). Strains of Group Ⅰ and Group II were closely related to Klebsiella sp.; Strain Ls8 of Group Ⅱ was a little far away from the genus of Pantoea (homology level 96% with Pantoea agglomerans), which may represent a new species or genus in Enterobacteraceae; Strains of Groups IV and V belonged to different Enterobacter sp.; Strain Ls4 and Ls 9 representing Group Ⅵ were close to Citrobacter amalonaticus with 98% sequence similarity; Strain Ls15 of Group Ⅶ showed 98% sequence identity with Pantoea sp.; Strains of Group Ⅷ were assigned to the genus Ideonella (β-proteobacteria). Based on the above results, endophytic diazotrophs isolated from O. rufipogon showed great diversity and some diazotrophs showed high nitrogenase activity with 42.52 μmol/mL·h C2H4. Inoculation to rice tests indicated that the isolated endophytic diazotrophs significantly promoted the rice growth.TAN ZhiYuan PENG GuiXiang XU PeiZhi AI ShaoYing TANG ShuanHu ZHANG GuoXia ZENG FengYun 2009Chinese Science Bulletin2009,54,16:14
4Stable chloroplast transformation of immature scutella and inflorescences in wheat (Triticum aestivum L.)显示文摘在小麦的叶绿体转变被 scutella 的轰炸分别地从不成熟的胚胎和不成熟的开花期完成。小麦叶绿体地点特定的表示向量, pBAGNRK,被放包含新霉素 phosphotransferase II (nptII ) 和绿色的一个表示盒子构造荧光灯蛋白质(gfp ) 作为选择和记者基因,分别地在在小麦的 atpB 和 rbcL 之间的 intergenic 分隔符叶绿体染色体。在 plastome 的 gfp 基因的集成被聚合酶链反应(PCR ) 识别分析并且作为一根探针的用 gfp 基因的南部的弄污。GFP 蛋白质的表示被西方的污点检验。三积极 transformants 被获得, atpB 和 rbcL (指向的地点) 的部分碎片的南部的污点探查证实他们之一是 homoplasmic。GFP 荧光的稳定的表示被共焦的显微镜学从 T1 子孙幼苗在叶纸巾证实。gfp 基因的 PCR 分析也在 T1 子孙证实了 transgene 的继承。这些结果加强小麦叶绿体转变的可行性并且也通过叶绿体转变在小麦为重要农学的特点的介绍给一个新奇方法。Cuiju Cui FeiSong Yi Tan Xuan Zhou Wen Zhao Fengyun Ma Yunyi Liu Javeed Hussain Yuesheng Wang Guangxiao Yang Guangyuan He 2011Acta Biochimica et Biophysica Sinica2011,43,4:10
5Further Recognition of Petroleum Exploration Potential of Marine Carbonates in Western Tarim Basin显示文摘A series of significant discoveries in marine carbonate rocks show great petroleum exploration potential in the Tarim Basin. However, the oil and gas fields discovered in the carbonate rocks are mainly distributed around the Manjiaer Sag in the eastern Tarim Basin. Some explorations occurred and no oil or gas field was discovered around the Awati Sag in the western Tarim Basin. Information from wells and outcrops reveals that there are excellent oil and gas source rock conditions around the Awati Sag. Transformed reef-shoal reservoirs could be formed in the Ordovician carbonate rocks with paleo-geographic background and hydrothermal conditions. Therefore, it is necessary to make a systematical study and overall evaluation of the potential of the periphery of the Awati Sag in terms of source rock evolution, resource potential, high-grade reservoir formation and distribution, and main factors controlling hydrocarbon migration and accumulation.Lü Xiuxiang Yang Haijun Yang Ning Zhao Fengyun Ma Yujie 2007Petroleum Science2007,4,3:8
6Size control of monodisperse nonporous silica particles by seed particle growth显示文摘Monodisperse nonporous silica particles were prepared by sol-precipitation via seed particle growth method,and the particle size,which varied from 1.0 to 4.7 m,was strictly controlled in our experiment. The formation of secondary particles,which resulted in a multimodal distribution of particle size,was suppressed by changing tetraethoxysilane(TEOS) concentration and reaction temperature. Furthermore,the effect of adding small amounts of electrolyte to the hydrolysis mixture was examined.Beibei Zhao Caihua Tian Yan Zhang Tao Tang Fengyun Wang 2011Particuology2011,9,3:7
7Heritable gene-targeting with gRNA/Cas9 in rats显示文摘Xinli Hu Nannan Chang Xuelian Wang Fengyun Zhou Xiaohai Zhou Xiaojun Zhu Jing-Wei Xiong 2013Cell Research2013,23,11:7
8Preparation of high-purity monodisperse silica microspheres by the sol-gel method coupled with polymerization-induced colloid aggregation显示文摘Ultra-pure mesoporous silica microspheres with good monodispersity were synthesized in two steps:nanometer-sized silica sol was produced by the sol-gel process,then micrometer-sized silica microspheres were synthesized by polymerization-induced colloid aggregation of the silica sol.The total metal content of the microspheres was extremely low,which eliminated the tailing of chromatographic peaks by chelating reagents.The pore structure of the silica microspheres could be controlled by altering the sol-gel conditions.The silica microsphere particle size could be adjusted by using different polymerizationinduced colloid aggregation conditions.Beibei Zhao Yan Zhang Tao Tang Fengyun Wang Tong Li Qiyu Lu 2015Particuology2015,13,5:7
9Carbon and nitrogen isotopes analysis and sources of organic matter in the upper reaches of the Chaobai River near Beijing,China显示文摘The carbon and nitrogen isotopes in the surface sediments,plants,and soil in the upper reaches of the Chaobai River have been researched.The results showed 27.75‰-21.58‰ and 1.32‰-6.74‰ for carbon and nitrogen isotopic ratios in the surface sediments,respectively.The sources of sedimentary organic matter in this area are soil organic matter,aquatic vascular plants,and riverine plankton,respectively,and a significant contributor to sediment in the Chaohe River,the Baihe River,and the Miyun Reservoir areas is soil organic matter.Furthermore,part of sedimentary organic matter in the Miyun Reservoir is attributed to the input from the Chaohe River and the Baihe River,the other is from C4 vegetation growing around individual point stations at the Miyun Reservoir.Compared with the situation in Hebei Province,the contribution of soil organic matter decreased significantly and river plankton and aquatic vascular plants increased significantly in Beijing municipal areas.This study reveals that the source of organic matter has a close relationship with the soil erosion.LU FengYun LIU ZhuQing JI HongBing 2013Science China Earth Sciences2013,56,2:6
10Dynamic modeling of hydrostatic guideway considering compressibility and inertia effect显示文摘静水力学的 guideways 被用作一种选择由于高僵硬并且在重机床的高抑制联系轴承。为了改进适用的动态特征,组织,静水力学的 guidway 的动态建模应该精确地被知道。这份报纸就惯性的效果而言论述一个 mass-spring-Maxwell 模型,压榨,压缩的可能性并且静态的适用。决定动态模型系数,在排水量和油电影的动态力量之间的不同诉讼的数字模拟与流畅的代码被执行。当它受到一个小摆动振幅时,模拟结果证明静水力学的 guidway 能作为一个线性系统被拿。基于一个动态模型和数字模拟,每个动态模型参数被 Levenberg-Marquardt 算法计算。鉴定结果证明那个 mass-spring-damper 模型是静水力学的 guidway 的最适当的动态模型。这份报纸为类似的静水力学的轴承的动态模型的分析提供一本参考书和准备。Yikang DU Kuanmin MAO Yaming ZHU Fengyun WANG Xiaobo MAO Bin LI 2015Frontiers of Mechanical Engineering2015,10,1:6
11Role of Microtubule-Associated Protein in Autism Spectrum Disorder显示文摘Autism spectrum disorder(ASD) is a neurodevelopmental disorder characterized by deficits in social interaction and communication, along with repetitive and restrictive patterns of behaviors or interests. Normal brain development is crucial to behavior and cognition in adulthood. Abnormal brain development, such as synaptic and myelin dysfunction, is involved in the pathogenesis of ASD. Microtubules and microtubule-associated proteins(MAPs) are important in regulating the processes of brain development, including neuron production and synaptic formation, as well as myelination. Increasing evidence suggests that the level of MAPs are changed in autistic patients and mouse models of ASD. Here, we discuss the roles of MAPs.Qiaoqiao Chang Hua Yang Min Wang Hongen Wei Fengyun Hu 2018Neuroscience Bulletin2018,34,6:6
12Experimental study of mode distortion induced by stimulated Raman scattering in high-power fiber amplifiers显示文摘The experimental investigation of mode distortion induced by stimulated Raman scattering(SRS)in a high-power fiber amplifier,which includes the evolutions of optical spectra,spatial beam profiles,and time-frequency characteristics,has been carried out in detail.Temporal-frequency characteristics have been studied for the first time,to the best of our knowledge,by using a low-speed camera and high-speed photodiode traces,which revealed that temporal-frequency characteristics of SRS-induced mode distortion are different from traditional dynamic mode instability(MI).The experimental results show that the output beam profile remains stable before the mode distortion occurs and fluctuates obviously after the onset of SRS-induced MI but on a time scale of seconds,which is much lower than that of Yb-gain-induced MI featuring millisecond-level beam profile fluctuation.It also shows that the mode distortion became measurable in company with the onset of inter-mode four-wave mixing(IM-FWM)when the ratio of Raman light reaches 3%;further,the beam quality factor M2degrades gradually from 1.4 to 2.1 as the ratio of Raman light increases.The mode distortion is accompanied by an obvious temperature increase of the output passive fiber,which further confirms that the mode distortion originates from SRS.The cause of the mode distortion induced by SRS has been explained in the context of core-pumped SRS effect,and the investigation on the accompanying IM-FWM effect indicates that the main content of the SRSinduced high-order mode is the LP21 mode.QIUHUI CHU QIANG SHU ZENG CHEN FENGYUN LI DONGLIN YAN CHAO GUO HONGHUAN LIN JIANJUN WANG FENG JING CHUANXIANG TANG RUMAO TAO 2020Photonics Research2020,8,4:6
13A generalized Markov chain model based on generalized interval probability显示文摘In the traditional Markov chain model (MCM), aleatory uncertainty because of inherent randomness and epistemic uncertainty due to the lack of knowledge are not differentiated. Generalized interval probability provides a concise representation for the two kinds of uncertainties simultaneously. In this paper, a generalized Markov chain model (GMCM), based on the generalized interval probability theory, is proposed to improve the reliability of prediction. In the GMCM, aleatory uncertainty is represented as probability; interval is used to capture epistemic uncertainty. A case study for predicting the average dynamic compliance in machining processes is provided to demonstrate the effectiveness of proposed GMCM. The results show that the proposed GMCM has a better prediction performance than that of MCM.XIE FengYun WU Bo HU YouMin WANG Yan 2013Science China(Technological Sciences)2013,56,9:6
14Synthesis,biological activities and 3D-QSAR studies of(R)-2-phenyl-4,5-dihydrothiazole-4-carboxamide derivatives containing a sulfur ether moiety显示文摘A series of(R)-2-phenyl-4,5-dihydrothiazole-4-carboxamide derivatives containing a sulfur ether moiety were synthesized and characterized on the basis of NMR and elemental analysis(EA). The crystal structure of(R)-N-(2-methyl-1-(methylthio)propan-2-yl)-2-(4-nitrophenyl)-4,5-dihydrothiazole-4-carboxamide(13 d) was determined to show R configuration. The bioasssy results indicated that most title compounds displayed good and broad spectrum antifungal activities against several phytopathogenic fungi. The structure activity relationships were discussed. Based on the antifungal activity of title compounds against Phytophthora capsici, a CoMSIA calculation was performed to establish a 3 D-QSAR model, which revealed that electrostatic and hydrophobic fields were the two most significant factors for antifungal activity. According to the established 3D-QSAR model, structure optimization was carried out to find(R)-N-((R)-1-(methylthio)propan-2-yl)-2-(p-tolyl)-4,5-dihydrothiazole-4-carboxamide(15 h)with excellent activity against Phytophthora capsici, thus emerging as a new lead compound for novel antiphytopathogenic fungus agent development.Jingbo Liu Fengyun Li Yuanhong Wang Haoxuan Zhang Jingyue Dong Pengwei Sun Yuxin Li Zhengming Li 2019Chinese Chemical Letters2019,30,3:5
15KHCO_3 activated carbon microsphere as excellent electrocatalyst for VO^(2+)/VO_2^+ redox couple for vanadium redox flow battery显示文摘In this paper,carbon microsphere prepared by hydrothermal treatment was activated by KHCO_3 at high temperature,and employed as the catalyst for VO^(2+)/VO_2^+redox reaction for vanadium redox flow battery(VRFB).Carbon microsphere can be etched by KHCO_3 due to the reaction between the pyrolysis products of KHCO_3 and carbon atoms.Moreover,KHCO_3 activation can bring many oxygen functional groups on carbon microsphere,further improving the wettability of catalyst and increasing the active sites.The electrocatalytic properties of carbon microsphere from hydrothermal treatment are improved by high temperature carbonization,and can further be enhanced by KHCO_3 activation.Among carbon microsphere samples,the VO^(2+)/VO_2^+redox reaction exhibits the highest electrochemical kinetics on KHCO_3 activated sample.The cell using KHCO_3 activated carbon microsphere as the positive catalyst demonstrates higher energy efficiency and larger discharge capacity,especially at high current density.The results reveal that KHCO_3 activated carbon microsphere is an efficient,low-cost carbon-based catalyst for VO^(2+)/VO_2^+redox reaction for VRFB system.Chen Zhao Yuehua Li Zhangxing He Yingqiao Jiang Lu Li Fengyun Jiang Huizhu Zhou Jing Zhu Wei Meng Ling Wang Lei Dai 2019Journal of Energy Chemistry2019,28,2:5
16Characterization of char from high temperature fluidized bed coal pyrolysis in complex atmospheres显示文摘Mei Zhong Shiqiu Gao Qi Zhou Junrong Yue Fengyun Ma Guangwen Xu 2016Particuology2016,14,2:5
17Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells显示文摘Phosphofructokinase-1(PFK-1),a major regulatory glycolytic enzyme,has been implicated in the functions of astrocytes and neurons.Here,we report that PFK-1 negatively regulates neurogenesis from neural stem cells(NSCs)by targeting pro-neural transcriptional factors.Using in vitro assays,we found that PFK-1 knockdown enhanced,and PFK-1 overexpression inhibited the neuronal differentiation of NSCs,which was consistent with the findings from NSCs subjected to 5 h of hypoxia.Meanwhile,the neurogenesis induced by PFK-1 knockdown was attributed to the increased proliferation of neural progenitors and the commitment of NSCs to the neuronal lineage.Similarly,in vivo knockdown of PFK-1 also increased neurogenesis in the dentate gyrus of the hippocampus.Finally,we demonstrated that the neurogenesis mediated by PFK-1 was likely achieved by targeting mammalian achaete-scute homologue-1(Mash 1),neuronal differentiation factor(NeuroD),and sex-determining region Y(SRY)-related HMG box 2(Sox2).All together,our results reveal PFK-1 as an important regulator of neurogenesis.Fengyun Zhang Xiaodan Qian Cheng Qin Yuhui Lin Haiyin Wu Lei Chang Chunxia Luo Dongya Zhu 2016Neuroscience Bulletin2016,32,3:4
18Mycobacterium bovis bacille Calmette-Guérin (BCG) enhances human β-defensin-1 gene transcription in human pulmonary gland epithelial cells显示文摘AIM: To examine the stimulatory effect of bacille Calmette-Guérin (BCG) cell wall components on human [β-defensin-1 (hBD-1)gene expression and analyze the response element in the 5'-flanking region of the gene. METHODS:BCG cell wall proteins were fractionated by Sephadex G-150 chromatography. Using reverse-transcription polymerase chain reaction (RT-PCR) and Northern hybridization analysis, hBD-1 mRNA expression was detected in ahuman pulmonary gland epithelial cell line SPC-A-1 cells. Progressive deletions of 5'-flanking region of hBD-1 genewere produced by PCR and ligated into promoterless chloramphenicol acetyltransferase (CAT) expression plasmidto construct pCAT reporter plasmids. Reporter gene expression was determined by ELISA. RESULTS: Therewas an obvious enhancement of hBD-1 mRNA expression after stimulation with heat-inactivated BCG whole cells(50mg/L), or the cell wall components with a molecular weight of 18-30 kDa (3mg/L) for 8 h. The upstreamsequence between -314 bp and +54 bp had the inducible activity by BCG, which contained CCAAT/enhancerbinding protein-β (C/EBPβ), activator protein-1 (AP-1), and CP2 cis element. CONCLUSION: BCG cell wallcomponents (18-30 kDa) can stimulate hBD-1 mRNA expression in pulmonary gland epithelial cells. The sequence(-314/+54) containing C/EBPβ, AP-1, and CP2 binding sites in the upstream of hBD-1 is involved in this induction.ZHUBing-Dong FENGYun HUANGNing, WUQi WANGBo-Yao 2003Acta Pharmacologica Sinica2003,24,9:4
19Bioaccumulation, subcellular distribution and chemical forms of yttrium in rice seedling显示文摘Yttrium(Y) accumulation in soil is a serious environment problem in China. To understand the mechanisms involved in Y tolerance and detoxification in rice seedling, the bioaccumulation, subcellular distribution, and chemical forms of Y was investigated in the present study. The results show that the content of Y both in roots and shoots increases with the increasing concentration of Y, and a large amount of Y is stored in roots. Subcellular distribution of Y in rice indicates that the majority of Y is bound to cell wall. Meanwhile, various chemical forms of Y is absorbed by rice. The greatest amount of Y is extracted by 2% hydrate acetic acid(HAc) and 0.6 mol/L HCl(particularly 2% HAc), which indicates that most of Y is combined with un-dissolved phosphate and oxalate(particularly phosphate). Cluster analysis, based on Y abundance levels, reveals the relationship between Y distribution patterns and chemical forms. Our results imply that Y forming of precipitates with phosphate(and/or oxalate) and depositing in the cell wall may be a key strategy for Y detoxicity and tolerance in rice.Shengnan Zheng Caiyun Zhang Kailun Shi Jinxiao Wang Guanjun Sun Qiaochu Hu Fengyun Zhao Xue Wang 2018Journal of Rare Earths2018,36,3:4
20North-south Differentiation of the Hydrocarbon Accumulation Pattern of Carbonate Reservoirs in the Yingmaili Low Uplift,Tarim Basin,Northwest China显示文摘摘要:由分析开发,结构的进化和剩余碳酸盐阶层的水库床的特征,这研究证明剩余在 Yingmaili 焦化阶层低高举在 Tabei 是有利的油和煤气的累积系列(北 Tarim 高举) 高举。有 Yingmaili 的北、南部的斜坡上的烃累积的不同模式低高举。油水库的纵贯的区别被剩余碳酸盐阶层和关键限制的不同岩性学在水库床的发展引起。在 Kuqa 消沉的中生代陆上的有机物和在 Manjiaer 的 Palaeozoic 海洋的有机物北消沉下垂分别地是为北斜坡和南部的斜坡的主要的烃来源岩石。北、南部的斜坡上的烃累积被差别在成熟和这些的热进化历史控制二种有机物。在南部的斜坡上,在早阶段形成的油累积完全被破坏,并且到 Himalayian 的从迟了的 Hercynian 的时期是烃累积的最重要的时间。然而,北斜坡上的烃累积的时间开始了内部埋葬的背斜水库是的 5 妈 B.P 碳酸盐在南部的斜坡上在场,当捱过时,外壳和埋葬 paleo 的山石灰岩地区常见的地形焦化水库在北斜坡上是在场的。北、南部的斜坡分别地有烃累积的不同控制因素。在水库床上的破裂生长是南部的斜坡上的最重要的控制因素;当北斜坡上的烃累积被捱过的外壳和帽子岩石控制时。LU Xiuxiang LI Jianjiao ZHAO Fengyun YANG Ning ZHANG Qiucha 2008Acta Geologica Sinica(English Edition)2008,82,3:4
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