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| 1 | The effect of land use structure on the distribution of soil nutrients in the hilly area of the Loess Plateau,China显示文摘The irrational land use is one of the main reasons for the soil erosion and nutrient loss in the loess hilly area of China. In this project, 4 types of typical land use structure of sustain ment for about 15 years in the loess hill slope are selected to study the effect of land use structure on the distribution of soil nutrients. From hill bottom to hill top, the patterns of land use types are:, grassland-slope farmland-forest, slope farmland-grassland-forest, terrace-grassland-forest and slope farmland-forest-grassland. By measuring the contents of the total N, total P, available N, available P and organic matter of soils, the results show that the land use structure types of slope farmland-grassland-forest and terrace-grassland-forest have a better capacity to maintain the soil nutrients. | Bojie Fu Keming Ma Huafeng Zhou Liding Chen | 1999 | Chinese Science Bulletin1999，44，8： | 32 |
| 2 | OsGRF4 controls grain shape, panicle length and seed shattering in rice显示文摘Traits such as grain shape, panicle length and seed shattering, play important roles in grain yield and harvest. In this study, the cloning and functional analysis of PANICLE TRAITS 2(PT2), a novel gene from the Indica rice Chuandali(CDL), is reported. PT2 is synonymous with Growth-Regulating Factor 4(OsGRF4), which encodes a growth-regulating factor that positively regulates grain shape and panicle length and negatively regulates seed shattering. Higher expression of OsGRF4 is correlated with larger grain, longer panicle and lower seed shattering. A unique OsGRF4 mutation, which occurs at the Osmi RNA396 target site of OsGRF4, seems to be associated with high levels of OsGRF4 expression, and results in phenotypic difference. Further research showed that OsGRF4 regulated two cytokinin dehydrogenase precursor genes(CKX5 and CKX1) resulting in increased cytokinin levels,which might affect the panicle traits. High storage capacity and moderate seed shattering of OsGRF4 may be useful in high-yield breeding and mechanized harvesting of rice. Our Resefindings provide additional insight into the molecular basis of panicle growth. | Pingyong Sun Wuhan Zhang Yihua Wang Qiang He Fu Shu Hai Liu Jie Wang Jianmin Wang Longping Yuan Huafeng Deng | 2016 | Journal of Integrative Plant Biology2016，58，10： | 16 |
| 3 | Fine mapping of GS2, a dominant gene for big grain rice显示文摘Grain shape as a major determinant of rice yield and quality is widely believed to be controlled by quantitative trait loci(QTL). We have identified a novel gene 'GS2' to largely regulate grain length and width in rice. The GS2 allele in the big-grain rice line ‘CDL’functioned in a dominant manner. In the present study, we employed a chromosome walking strategy in the residual heterozygous lines from recombinant inbred population between cultivar 'R1126' and CDL, and located the GS2 gene in an interval of ~33.2 kb flanked by marker GL2-35-1 and GL2-12 in the long arm of rice chromosome 2. According to genome annotations, three putative gene loci, LOC_Os02g47280, LOC_Os02g47290 and LOC_Os02g47300, exist in this candidate region. In addition, allelic analysis with previously reported genes demonstrated that GS2 was novel for regulating rice grain shape. These results will help promote the cloning and functional characterization of the GS2 gene and further develop linked markers to be used in marker-assisted breeding. | Wuhan Zhang Pingyong Sun Qiang He Fu Shu Jie Wang Huafeng Deng | 2013 | The Crop Journal2013，1，2： | 7 |
| 4 | Natural polypeptides treat pollution complex: Moistureresistant multi-functional protein nanofabrics for sustainable air filtration显示文摘有象优秀过滤效率, eco 友好,和环境稳定性那样的特征的绿多功能的空气过滤器的开发极其被要求探讨弄脏的空气的增加的担心。自然蛋白质例如酱油蛋白质和明胶,作为由于富有的功能的组的多功能的空气过滤材料是吸引人的候选人;然而,这些简历材料对潮湿脆弱,它在实践限制他们的宽广申请。在这个工作,从丰富的玉米导出的玉米朊的恐水病的蛋白质第一次被修改经由 electrospinning 生产高效的 nanofilters。玉米朊 nanofabrics 在无毒的溶剂和 co-electrospinning 代理人的帮助下被制作, poly (乙烯氧化物) 。结果表明基于玉米朊的 nanofabrics 为从 0.1 ~ 10 m 的不同尺寸的微粒事的同时的移动显示出高效率(>99.5%) 并且某些气体的有毒的化学药品(>70%) 。另外,到纤维素纸巾的优秀潮湿抵抗和好粘附用作空气过滤器底层的玉米朊 nanofabrics 表演。这研究基于象玉米朊那样的蛋白质为开发多功能的绿空气过滤器正在答应材料的恐水病的天赋表明那 nanofabrics。 | Huafeng Tian Xuewei Fu Min Zheng Yu Wang Yichao Li Aimin Xiang Wei-Hong Zhong | 2018 | Nano Research2018，11，8： | 5 |
| 5 | Voltammetric studies on free radicals of lower fatty alcohols显示文摘In this work, both generation and reduction processes of the a-radicals of lower fatty alcohols were studied by voltarametry in lower fatty alcohols/peroxydisul-fate/phosphate buffer system. Experimental results showed that such three reactions were successively completed at negative potential on mercury electrode surface: (i) sulfate radical anion SO4- was generated electrochemically from one-electron reduction of peroxydisulfate ion S2O82-,(ii) the SO4- oxidized lower fatty alcohols to the corresponding a-radicals and (iii) the a-radicals were reduced in one-electron and one-proton addition to produce their own reduction waves. The apparent free energy △rGm of the a-radicals of lower straight-chain fatty alcohols such as methanol, ethanol, propanol, butanol and pentanol obtained were 107.09, 116.74, 122.53, 126.39 and 129.28 kJ.mol/L, respectively. The proposed method is more simple and convenient than the current approaches based on the radical-chain reaction for studying alcohol oxidation. | SONG Junfeng FU Huafeng GUO Wei | 2001 | Chinese Science Bulletin2001，46，15： | 2 |
| 6 | The relationships between land use and soil conditions in the hilly area of the loess plateau in northern Shaanxi, China显示文摘 | Bojie Fu Liding Chen Keming Ma Huafeng Zhou Jun Wang | 2000 | Catena2000，，1： | 1 |
| 7 | Development and Application of a Novel Functional Marker for Fragrance in Rice显示文摘Fragrant rice is favored worldwide by consumers and rice breeders,due to its full aroma and high nutritional value.Loss of function of the betaine aldehyde dehydrogenase gene(OsBADH2)leads to aroma in rice.Our previous sequencing results showed that fragrant rice Chuan 7 belongs to badh2-E14 type,with a 1 bp insertion mutation in exon 14(Sun et al,2021),and this allele mutation is mainly distributed in Nepal and India(Kovach et al,2009). | SUN Pingyong ZHANG Wuhan ZHANG Li SHU Fu HE Qiang XU Na PENG Zhirong ZENG Jia FANG Pengpeng DENG Huafeng | 2023 | Rice science2023，30，3： | 0 |
| 8 | TEAseq-based identification of 35,696 Dissociation insertional mutations facilitates functional genomic studies in maize显示文摘In plants,transposable element(TE)-triggered mutants are important resources for functional genomic studies.However,conventional approaches for genome-wide identification of TE insertion sites are costly and laborious.This study developed a novel,rapid,and high-throughput TE insertion site identification workflow based on next-generation sequencing and named it Transposable Element Amplicon Sequencing(TEAseq).Using TEAseq,we systemically profiled the Dissociation(Ds)insertion sites in 1606 independent Ds insertional mutants in advanced backcross generation using K17 as background.The Ac-containing individuals were excluded for getting rid of the potential somatic insertions.We characterized 35,696 germinal Ds insertions tagging 10,323 genes,representing approximately 23.3%of the total genes in the maize genome.The insertion sites were presented in chromosomal hotspots around the ancestral Ds loci,and insertions occurred preferentially in gene body regions.Furthermore,we mapped a loss-of-function AGL2 gene using bulked segregant RNA-sequencing assay and proved that AGL2 is essential for seed development.We additionally established an open-access database named MEILAM for easy access to Ds insertional mutations.Overall,our results have provided an efficient workflow for TE insertion identification and rich sequence-indexed mutant resources for maize functional genomic studies. | Mingjie Lyu Huafeng Liu Joram Kiriga Waititu Ying Sun Huan Wang Junjie Fu Yanhui Chen Jun Liu Lixia Ku Xiliu Cheng | 2021 | Journal of Genetics and Genomics2021，48，11： | 0 |
| 9 | PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation显示文摘Tumor metabolic reprogramming and epigenetic modification work together to promote tumorigenesis and development.Protein lysine acetylation,which affects a variety of biological functions of proteins,plays an important role under physiological and pathological conditions.Here,through immunoprecipitation and mass spectrum data,we show that phosphoglycerate mutase 5(PGAM5)deacetylation enhances malic enzyme 1(ME1)metabolic enzyme activity to promote lipid synthesis and proliferation of liver cancer cells.Mechanistically,we demonstrate that the deacetylase SIRT2 mediates PGAM5 deacetylation to activate ME1 activity,leading to ME1 dephosphorylation,subsequent lipid accumulation and the proliferation of liver cancer cells.Taken together,our study establishes an important role for the SIRT2-PGAM5-ME1 axis in the proliferation of liver cancer cells,suggesting a potential innovative cancer therapy. | Gongyu Fu Shi-Ting Li Zetan Jiang Qiankun Mao Nanchi Xiong Xiang Li Yijie Hao Huafeng Zhang | 2023 | Acta Biochimica et Biophysica Sinica2023，55，9： | 0 |
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