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| 1 | The effect of land use structure on the distribution of soil nutrients in the hilly area of the Loess Plateau,China显示文摘The irrational land use is one of the main reasons for the soil erosion and nutrient loss in the loess hilly area of China. In this project, 4 types of typical land use structure of sustain ment for about 15 years in the loess hill slope are selected to study the effect of land use structure on the distribution of soil nutrients. From hill bottom to hill top, the patterns of land use types are:, grassland-slope farmland-forest, slope farmland-grassland-forest, terrace-grassland-forest and slope farmland-forest-grassland. By measuring the contents of the total N, total P, available N, available P and organic matter of soils, the results show that the land use structure types of slope farmland-grassland-forest and terrace-grassland-forest have a better capacity to maintain the soil nutrients. | Bojie Fu Keming Ma Huafeng Zhou Liding Chen | 1999 | Chinese Science Bulletin1999，44，8： | 32 |
| 2 | Genome-wide CRISPR screen identifies synthetic lethality between DOCK1 inhibition and metformin in liver cancer显示文摘Metformin is currently a strong candidate anti-tumor agent in multiple cancers.However,its anti-tumor effectiveness varies among different cancers or sub-populations,potentially due to tumor heterogeneity.It thus remains unclear which hepatocellular carcinoma(HCC)patient subpopulation(s)can benefit from met-formin treatment.Here,through a genome-wide CRISPR-Cas9-based knockout screen,we find that DOCK1 levels determine the anti-tumor effects of met-formin and that DOCK1 is a synthetic lethal target of metformin in HCC.Mechanistically,metformin promotes DOCK1 phosphorylation,which activates RAC1 to facilitate cell survival,leading to metformin resistance.The DOCK1-selective inhibitor,TBOPP,potentiates anti-tumor activity by metformin in vitro in liver cancer cell lines and patient-derived HCC organoids,and in vivo in xenografted liver cancer cells and immunocompetent mouse liver cancer models.Notably,metformin improves overall survival of HCC patients with low DOCK1 levels but not among patients with high DOCK1 expression.This study shows that metformin effective-ness depends on DOCK1 levels and that combining metformin with DOCK1 inhibition may provide a promising personalized therapeutic strategy for met-formin-resistant HCC patients. | Junru Feng Hui Lu Wenhao Ma Wenjing Tian Zhuan Lu Hongying Yang Yongping Cai Pengfei Cai Yuchen Sun Zilong Zhou Jiaqian Feng Jiazhong Deng Ying Shu Kun Qu Weidong Jia Ping Gao Huafeng Zhang | 2022 | Protein & Cell2022，13，11： | 5 |
| 3 | Enhancement of oral bioavailability and anti-Parkinsonian efficacy of resveratrol through a nanocrystal formulation显示文摘Resveratrol(RES),a non-flavonoid polyphenol extracted from a wide variety of plants,exhibits neuroprotective activities against Parkinson’s disease(PD).However,undesirable water solubility of RES reduces its oral bioavailability and demonstrates lowefficacy in blood and brain,thus limiting its application.In present study,a nanocrystal formulation of RES(RES-NCs)was developed to enhance its oral bioavailability and delivery into brain for PD treatment.RES-NCs were fabricated with hydroxypropyl methylcellulose(HPMC)stabilizer via antisolvent precipitation approach.The obtained RES-NCs displayed the particle size of 222.54±1.66 nm,the PDI of 0.125±0.035,the zeta potential of−9.41±0.37mV,and a rapid in vitro dissolution rate.Molecular dynamics simulation of RES and HPMC revealed an interaction energy of−68.09 kJ/mol and a binding energy of−30.98±0.388 kJ/mol,indicating that the spontaneous binding between the two molecules is through van der Waals forces.RES-NCs conferred enhanced cellular uptake as well as improved permeability relative to pure RES.In addition,RES-NCs were able to protect neurons against cytotoxicity induced by MPP+.Meanwhile,RES-NCs exerted no significant toxic effects on zebrafish embryos and larvae,and did not influence their survival and hatching rates.When orally administered to rats,RES-NCs exhibited more favorable pharmacokinetics than pure RES,with higher plasma and brain concentrations.More importantly,MPTP-induced PD mice showed notable improvements in behavior,attenuated dopamine deficiency,and elevated levels of dopamine and its metabolites after the treatment with RES-NCs.Furthermore,immunoblot analysis revealed that the neuroprotective role of RES-NCsmay be at least partially mediated by Akt/Gsk3βsignaling pathway.Taken altogether,RES-NCs can serve as a potential treatment modality for PD,offering means of improving RES oral bioavailability and brain accumulation. | Sha Xiong Wei Liu Yile Zhou Yousheng Mo Yao Liu Xiaojia Chen Huafeng Pan Dongsheng Yuan QiWang Tongkai Chen | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 3 |
| 4 | PROPERTY IDENTIFICATION OF SINGULARITY LOCI OF GOUGHSTEWART MANIPULATOR显示文摘鉴别 Gough-Stewartmanipulators 的奇特 loci 的性质被探讨的问题。在构造 Gough-Stewart 操纵者的 Jacobian 矩阵以后,在活动平台位置参数的一个立方的多项式表达式,代表操纵者的 theconstant 取向奇特地点,被导出。为不同取向的操纵者的奇特地点的图形的代表进一步与例子被说明操纵者在的奇特地点主要节,活动站台在哪儿躺,被分析。它在平行 principal-sectionsare 显示出操纵者的那奇特 loci 包括一根抛物线,四交叉直线和无限的双曲线的所有二次的表情。他们的几何、运动学的性质也也被研究。 | CAO Yi HUANG Zhen DING Huafeng ZHOU Hui | 2006 | Chinese Journal of Mechanical Engineering2006，19，1： | 2 |
| 5 | Progress of Optical Fiber Sensors and Its Application in Harsh Environment显示文摘Fiber sensors have been developed for industry application with significant advantages.In this paper,Fiber sensors for oil field service and harsh environment monitoring which have been investigated in Tsinghua University are demonstrated.By discussing the requirements of practical applications,the key technologies of long-period fiber grating(LPFG)based fiber sensor,optical spectrum analyzer for oil detection,laser induced breakdown spectroscopy(LIBS)system for soil contamination monitoring,and seismic sensor arrays are described. | Min ZHANG Xiaohong MA Liwei WANG Shurong LAI Hongpu ZHOU Huafeng ZHAO Yanbiao LIAO | 2011 | Photonic Sensors2011，1，1： | 2 |
| 6 | Anti-hyperuricemic and nephroprotective effects of Smilax china L.显示文摘 | Lvyi Chen Huafeng Yin Zhou Lan Shuwei Ma Chunfeng Zhang Zhonglin Yang Ping Li Baoqin Lin | 2011 | Journal of Ethnopharmacology2011，，2： | 1 |
| 7 | The relationships between land use and soil conditions in the hilly area of the loess plateau in northern Shaanxi, China显示文摘 | Bojie Fu Liding Chen Keming Ma Huafeng Zhou Jun Wang | 2000 | Catena2000，，1： | 1 |
| 8 | Blockade of LIGHT/HVEM and B7/CD28 Signaling Facilitates Long-Term Islet Graft Survival With Development of Allospecific Tolerance显示文摘 | Kexing Fan Hao Wang Huafeng Wei Qian Zhou Geng Kou Sheng Hou Weizhu Qian Jianxin Dai Bohua Li Yanyun Zhang Tongyu Zhu Yajun Guo | 2007 | Transplantation2007，，6： | 1 |
| 9 | Transmission Line Sequence Impedances Identification Using PMU Measurements显示文摘 | Huafeng Zhou Xuanyu Zhao Di Shi Huashi Zhao Chaoyang Jing | 2015 | Journal of Energy and Power Engineering2015，9，2： | 1 |
| 10 | On-Line PMU-Based Transmission Line Parameter Identification显示文摘China Southern Power Grid Company(CSG)recently developed and implemented an online PMU-based transmission line(TL)parameter identification system(TPIS).Traditionally,TL parameters are calculated based on transmission tower geometries,conductor dimension,estimates of line length,conductor sags,etc.These parameters only approximate the effect of conductor sag and ignore the dependence of impedance parameters on temperature variation.Recent development in PMU technology has made it possible to calculate TL parameters accurately.The challenges are that such an application requires highly accurate PMU data while the accuracy of PMU measurements under different working/system conditions can be uncertain.With a large number of PMUs widely installed in its system,CSG plans to improve and update the EMS database using the newly developed TPIS.TPIS provides an innovative yet practical problem formulation and solution for TL parameter identification.In addition,it proposes a new metric that can be used to determine the credibility of the calculated parameters,which is missing in the literature.This paper discusses the methodologies,challenges,as well as implementation issues noticed during the development of TPIS. | Xuanyu Zhao Huafeng Zhou Di Shi Huashi Zhao Chaoyang Jing Chris Jones | 2015 | CSEE Journal of Power and Energy Systems2015，1，2： | 1 |
| 11 | KDELR2 promotes breast cancer proliferation via HDAC3-mediated cell cycle progression显示文摘Background:Histone deacetylases(HDACs)engage in the regulation of various cellular processes by controlling global gene expression.The dysregulation of HDACs leads to carcinogenesis,making HDACs ideal targets for cancer therapy.However,the use of HDAC inhibitors(HDACi)as single agents has been shown to have limited success in treating solid tumors in clinical studies.This study aimed to identify a novel downstream effector of HDACs to provide a potential target for combination therapy.Methods:Transcriptome sequencing and bioinformatics analysis were performed to screen for genes responsive toHDACi in breast cancer cells.The effects of HDACi on cell viability were detected using the MTT assay.The mRNA and protein levels of genes were determined by quantitative reverse transcription-PCR(qRT-PCR)andWestern blotting.Cell cycle distribution and apoptosis were analyzed by flow cytometry.The binding of CREB1(cAMP-response element binding protein 1)to the promoter of the KDELR(The KDEL(Lys-Asp-Glu-Leu)receptor)gene was validated by the ChIP(chromatin immunoprecipitation assay).The association between KDELR2 and protein of centriole 5(POC5)was detected by immunoprecipitation.A breast cancer-bearing mouse model was employed to analyze the effect of the HDAC3-KDELR2 axis on tumor growth.Results:KDELR2 was identified as a novel target of HDAC3,and its aberrant expression indicated the poor prognosis of breast cancer patients.We found a strong correlation between the protein expression patterns of HADC3 and KDELR2 in tumor tissues from breast cancer patients.The results of the ChIP assay and qRT-PCR analysis validated that HDAC3 transactivated KDELR2 via CREB1.The HDAC3-KDELR2 axis accelerated the cell cycle progression of cancer cells by protecting the centrosomal protein POC5 from proteasomal degradation.Moreover,the HDAC3-KDELR2 axis promoted breast cancer cell proliferation and tumorigenesis in vitro and in vivo.Conclusion:Our results uncovered a previously unappreciated function of KDELR2 in tumorigenesis,linking a critical Golgi-the endoplasmic reticulum traffic transport protein to HDAC-controlled cell cycle progression on the path of cancer development and thus revealing a potential therapeutical target for breast cancer. | Haoran Wei Wenhao Ma Xiaofei Lu Haiying Liu Kashuai Lin Yinghui Wang Zijian Ye Linchong Sun Zhitong Huang Tingting Pan Zilong Zhou Eric Y.Cheng Huafeng Zhang Ping Gao Xiuying Zhong | 2021 | Cancer Communications2021，41，9： | 0 |
| 12 | The Genesis of the Damiao Granodiorite in Siziwangqi and Its Constraint on the Crust Evolution of Inner Mongolia显示文摘A large number of granitoids constitutes a giant granitic belt trending EW in the Inner Mongolia.As a representative of those intrusions,the Damiao granitoid in Siziwangqi area may serve as a natural laboratory for the study of genesis of granite and evolution of crust.It is mainly composed of mid-coarse grained granodiorite with abundant | Yongmei Zhang,Huafeng Zhang,Weiping Zhu,Wencan Liu,Zhiguang Zhou 1.China University of Geosciences(Beijing),Beijing 100083,China. 2.China Aero Geophysical Survey and Remote Sensing Center for Land and Resources,Beijing 100083,China | 2009 | 地学前缘2009，16，S1： | 0 |
| 13 | Measures of bioavailable serum sex hormone levels in aging Chinese by protein chip显示文摘The purpose of this study was to develop a protein chip technique based on receptor binding assays to measure bioavailable serum sex hormone levels (BSSHL). 224 aging healthy Chinese were investigated to get the referenced values of BSSHL for the first time. In the assays recombined sex hormone receptor proteins were jointed to polysaccharide coated slides to make protein chip, and the dose-dependence curves of sex hormone on chip were prepared. The data showed that this method had good precision (CV<16%) and accuracy (Bias<10%), and the sensitivity could reach 1 pmol/L. From the results, BSSHL of men and women declined with aging, but no significant differences were observed. The BSSHL of aging men were higher than those of women. The bioavailable serum androgen level of men was 52―112 pmol/L, women's was 3―70 pmol/L and the whole group was 41.9―81.4 pmol/L. The bioavailable serum estrogen level of men was 0.8―3.0 pmol/L, women's was 1.2―2.5 pmol/L and the whole group was 0.6―2.64 pmol/L. Based on the assays, BSSHL measurement by protein chip can meet the needs of epidemiological studies in terms of speed, accuracy and sample volume required, and was helpful in quantitative assessment of aging people's health. | ZHOU Yong, CHANG Shuying, MENG Xiaoluo, YU Huafeng, WANG Luning, HE Jinggui, ZHANG Baohe, ZHANG Juntian, GENG Meiyu & DU Guanhua Institute of Marine Drugs and Food, Ocean University of China, Qingdao 266003, China Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China General Hospital of Air Force, PLA, Beijing 100036, China 304 Hospital of PLA, Beijing 100037, China Beijing Tongren Hospital, Beijing 100730, China General Hospital of PLA, Beijing 100853, China | 2006 | Science China(Life Sciences)2006，49，3： | 0 |
| 14 | 肿瘤再生细胞通过犬尿氨酸转移以及芳香烃受体激活诱导CD8^+T细胞表达PD-1显示文摘文章简介肿瘤免疫治疗是当前肿瘤研究的热点领域之一,通过利用免疫细胞、免疫分子直接或间接杀伤肿瘤细胞,以控制或清除肿瘤,被认为是人类战胜癌症的希望所在。肿瘤免疫治疗主要依赖活化的T细胞杀伤肿瘤细胞. | 刘玉英 梁晓雨 董文茜 Yi Fang Jiadi Lv Tianzhen Zhang Roland Fiskesund Jing Xie Jinyan Liu Xiaonan Yin Xun Jin Degao Chen Ke Tang Jingwei Ma Huafeng Zhang Jing Yu Jun Yan Huaping Liang Siqi Mo Feiran Cheng Yabo Zhou Haizeng Zhang Jing Wang Jingnan Li Yang Chen Bing Cui Zhuo-Wei Hu Xuetao Cao F. Xiao-Feng Qin 黄波 | 2019 | 科学新闻2019，0，2： | 0 |
| 15 | Prediction of severe preeclampsia in machine learning显示文摘This study aimed to find out the blood data characteristics of patients and explore the correlation between severe preeclampsia and blood index value.Provide assistance for the early attention direction of severe preeclampsia diagnosis and treatment.19,653 pregnant women presenting to the West China Second University Hospital,Sichuan University from January 2017 to April 2019.After screening,a total of 248 patients,124 severe preeclampsia cases,and 124 controls were selected for this study.Forty-three blood examination variables were obtained from routine blood work,hepatic,renal and coagulation function examination.Light gradient boosting machine(light GBM),decision tree and random forest were used for date diving.We randomly divided 35%of the original data as a testing set to conduct internal validation of the performance of the prediction model.The area under receiver operating characteristic curve(AUC)was used as the main score to compare the three methods.Finally,a binary classification light GBM model based on aspartate aminotransferase,direct bilirubin and activated partial thromboplastin time ratio can predict severe preeclampsia with sensitivity of 88.37%,specificity of 77.27%,AUC of 89.74%and positive predictive value of 65.96%.We believe relevant quantifiable indicators can establish an effective prediction model,which can provide guidance for early detection and prevention of severe preeclampsia. | Xinyuan Zhang Yu Chen Stephen Salerno Yi Li Libin Zhou Xiaoxi Zeng Huafeng Li | 2022 | Medicine in Novel Technology and Devices2022，，3： | 0 |
| 16 | Increased expression of coronin-1a in amyotrophic lateral sclerosis: a potential diagnostic biomarker and therapeutic target显示文摘Amyotrophic lateral sclerosis(ALS)is the most common motor neuron disease.At present,no definite ALS biomarkers are available.In this study,exosomes from the plasma of patients with ALS and healthy controls were extracted,and differentially expressed exosomal proteins were compared.Among them,the expression of exosomal coronin-1a(CORO1A)was 5.3-fold higher than that in the controls.CORO1A increased with disease progression at a certain proportion in the plasma of patients with ALS and in the spinal cord of ALS mice.CORO1A was also overexpressed in NSC-34 motor neuron-like cells,and apoptosis,oxidative stress,and autophagic protein expression were evaluated.CORO1A overexpression resulted in increased apoptosis and oxidative stress,overactivated autophagy,and hindered the formation of autolysosomes.Moreover,CORO1A activated Ca^(2+)-dependent phosphatase calcineurin,thereby blocking the fusion of autophagosomes and lysosomes.The inhibition of calcineurin activation by cyclosporin A reversed the damaged autolysosomes.In conclusion,the role of CORO1A in ALS pathogenesis was discovered,potentially affecting the disease onset and progression by blocking autophagic flux.Therefore,CORO1A might be a potential biomarker and therapeutic target for ALS. | Qinming Zhou Lu He Jin Hu Yining Gao Dingding Shen You Ni Yuening Qin Huafeng Liang Jun Liu Weidong Le Sheng Chen | 2022 | Frontiers of Medicine2022，16，5： | 0 |
| 17 | Kinematic synthesis and simulation of a vegetable pot seedling transplanting mechanism with four exact task poses显示文摘To obtain a picking and planting integrated transplanting mechanism(PITM)with ideal trajectory shape,reasonable operating attitude,and compact structure for vegetable pot seedling transplanting,a symmetrical structural PITM composed of two planetary carriers and driven by a cam-noncircular gear combination mechanism was proposed in this paper.In accordance with the requirements of transplanting agronomy,the shape of the motion trajectory and the attitude of the end tip of the clamping claw at several specific positions(the starting and end points of picking seedling,the starting point of pushing seedling,and a special point on the return segment)were planned.To make the clamping claws of PITM pass through a set of prescribed positions,the mechanism was simplified to an open-chain 3R mechanism.An accurate four-position-based method for solving the open-chain mechanism model was adopted to obtain the curves of its fixed hinge point(central point)and dynamic hinge point(circle point).The ratio of adjacent bars’lengths was analyzed to determine the feasible interval of the central and circle point curves and the reasonable link length.A desired closed transplanting trajectory that goes through the given points and the corresponding transmission ratio model of the open-chain mechanism was established.After the transmission ratio of each stage of gear and cam pairs was calculated and distributed,the prototype of PITM was designed and manufactured.Lastly,the effectiveness and feasibility of the design were verified through a preliminary experiment of the prototype. | Liang Sun Haocong Xu Yuzhu Zhou Jiahao Shen Gaohong Yu Huafeng Hu Yuejun Miao | 2023 | International Journal of Agricultural and Biological Engineering2023，16，2： | 0 |
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