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| 1 | H5N1 influenza virus-specific miRNA-like small RNA increases cytokine production and mouse mortality via targeting poly(rC)-binding protein 2显示文摘H5N1 流行性感冒病毒的感染在所有流行性感冒病毒之中引起最高的死亡。位于如此的高病毒的致病力下面的机制不完全地被理解。这里,我们报导 H5N1 流行性感冒病毒编码象 microRNA 一样小 RNA, miR-HA-3p,它从茎被处理由 Argonaute 2 的包含环的病毒的 RNA 先锋,和戏在在 H5N1 感染期间提高 cytokine 生产的一个角色。miR-HA-3p poly 指向的机械学的学习表演(rC ) 有约束力的蛋白质 2 (PCBP2 ) 并且压制它的表达式。与 PCBP2 being 一致 RIG-I/MAVS-mediated 抗病毒的天生的免疫的一个重要否定管理者,由 miR-HA-3p 的 PCBP2 表示的抑制在感染 H5N1 的人的巨噬细胞和老鼠支持 cytokine 生产病毒。我们断定 miR-HA-3p 是首先识别的流行性感冒编码病毒的象 microRNA 一样功能的 RNA 碎片和贡献导致 H5N1 的 cytokine 暴风雨和死亡的一个新奇毒力因素。 | Xihan Li Zheng Fu Hongwei Liang Yanbo Wang Xian Qi Meng Ding Xinlei Sun Zhen Zhou Ying Huang Hongwei Gu Limin Li Xi Chen Donghai Li Quan Zhao Fenyong Li Hua Wang Jin Wang Ke Zen Chen-Yu Zhang | 2018 | Cell Research2018，28，2： | 12 |
| 2 | Indirectly extruded biodegradable Zn-0.05wt%Mg alloy with improved strength and ductility: In vitro and in vivo studies显示文摘As compared to permanent orthopedic implants for load-bearing applications, biodegradable orthopedic implants have the advantage of no need for removing after healing, but they suffer from the 'trilemma' problem of compromising among sufficiently high mechanical properties, good biocompatibility and proper degradation rate conforming to the growth rate of new bones. In the present work, in vitro and in vivo studies of a Zn-0.05 wt%Mg alloy(namely, Zn-0.05 Mg alloy) were conducted with pure Zn as a control. The Zn-0.05 Mg alloy is composed of a small amount of Mg_2 Zn11 phase embedded in the refined Zn matrix with an average grain size of ~20 μm. The addition of 0.05 wt% Mg into Zn significantly increases the ultimate tensile strength up to 225 MPa and the elongation to fracture to 26%, but has little influence on the in vitro degradation rate. Both Zn and Zn-0.05 Mg alloy exhibit homogeneous in vitro degradation with a rate of about 0.15 mm/year. Based on the cytotoxicity evaluation, Zn and Zn-0.05 Mg alloy do not induce toxicity to L-929 cells, indicating that they have little toxicity to the general functions of the animal. An in vivo biocompatibility study of Zn and Zn-0.05 Mg alloy samples by placing them in a rabbit model for 4.12 and 24 weeks, respectively did not show any inflammatory cells, and demonstrated that new bone tissue formed at the bone/implant interface, suggesting that Zn and Zn-0.05 Mg alloy promote the formation of new bone tissue. The in vivo degradation of Zn and Zn-0.05 Mg alloy does not bring harm to the important organs and their cell structures. More interestingly, Zn and Zn-0.05 Mg alloy exhibit strong antibacterial activity against Escherichia coli and Staphylococcus aureus. The above results clearly demonstrate that the Zn-0.05 Mg alloy could be a potential biodegradable orthopedic implant material. | Chi Xiao Liqing Wang Yuping Ren Shineng Sun Erlin Zhang Chongnan Yan Qi Liu Xiaogang Sun Fenyong Shou Jingzhu Duan Huang Wang Gaowu Qin | 2018 | Journal of Materials Science & Technology2018，34，9： | 11 |
| 3 | Detection of congenital cytomegalovirus in newborns using nucleic acid amplification techniques and its public health implications显示文摘Human cytomegalovirus(HCMV), a herpesvirus, is an important human pathogen that causes asymptomatic infections in healthy or immunocompetent individuals but can lead to severe and potentially life-threatening complications in immune-immature individuals such as neonates or immune-compromised patients such as organ-transplant recipients and HIV-positive individuals.Congenital HCMV infection represents a significant public health issue and poses substantial healthcare and economic burden to society. This virus causes the most common viral congenital infection worldwide, and is the leading non-genetic cause of sensorineural hearing loss in children in developed countries. Congenital HCMV infection is believed to fulfill the criteria of the American College of Medical Genetics to be considered as a condition targeted for a newborn screening program. This is because congenital HCMV infection can be identified during a time(within 2 days after birth) at which it would not ordinarily be detected clinically, and there are demonstrated benefits of early detection, timely intervention, and efficacious treatment of the condition. Recent progresses in developing polymerase chain reaction-based approaches to detect HCMV in samples obtained from newborns have generated much excitement in the field. In this review, we highlight the recent progress in diagnostic techniques that could potentially be used for the detection of HCMV infection in neonates and its direct implications in public health settings for diagnosing congenital HCMV infection. | Guoyu Liu Rong Hai Fenyong Liu | 2017 | Virologica Sinica2017，32，5： | 4 |
| 4 | Detection of the Pandemic H1N1/2009 Influenza A Virus by a Highly Sensitive Quantitative Real-time Reverse-transcription Polymerase Chain Reaction Assay显示文摘A quantitative real time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers recommended by the World Health Organization (WHO) has been widely used successfully for detection and monitoring of the pandemic H1N1/2009 influenza A virus. In this study, we report the design and characterization of a novel set of primers to be used in a qRT-PCR assay for detecting the pandemic H1N1/2009 virus. The newly designed primers target three regions that are highly conserved among the hemagglutinin (HA) genes of the pandemic H1N1/2009 viruses and are different from those targeted by the WHO-recommended primers. The qRT-PCR assays with the newly designed primers are highly specific, and as specific as the WHO-recommended primers for detecting pandemic H1N1/2009 viruses and other influenza viruses including influenza B viruses and influenza A viruses of human, swine, and raccoon dog origin. Furthermore, the qRT-PCR assays with the newly designed primers appeared to be at least 10-fold more sensitive than those with the WHO-recommended primers as the detection limits of the assays with our primers and the WHO-recommended primers were 2.5 and 25 copies of target RNA per reaction, respectively. When tested with 83 clinical samples, 32 were detected to be positive using the qRT-PCR assays with our designed primers, while only 25 were positive by the assays with the WHO-recommended primers. These results suggest that the qRT-PCR system with the newly designed primers represent a highly sensitive assay for diagnosis of the pandemic H1N1/2009 virus infection. | Zhu Yang Guoliang Mao Yujun Yuan-Chuan Chen Chengjing Liu Jun Luo Xihan Li Ke Zen Yanjun Pang Jianguo Wu Fenyong Liu | 2013 | Virologica Sinica2013，28，1： | 2 |
| 5 | Mutual inhibition between YAP and SRSF1 maintains long non-coding RNA, Malat1-induced tumourigenesis in liver cancer显示文摘 | Jiayi Wang Hongmei Wang Yue Zhang Ni Zhen Li Zhang Yongxia Qiao Wenhao Weng Xiangfan Liu Lifang Ma Weifan Xiao Wenjun Yu Qinghua Chu Qiuhui Pan Fenyong Sun | 2014 | Cellular Signalling2014，，： | 1 |
| 6 | Characterization of function and regulation of miR-24-1 and miR-31显示文摘 | Fenyong Sun Jiayi Wang Qiuhui Pan Yongchun Yu Yue Zhang Yang Wan Ju Wang Xiaoyan Li An Hong | 2009 | Biochemical and Biophysical Research Communications2009，，3： | 1 |
| 7 | TFCP2蛋白为YAP发挥转录依赖性促肝癌效应所必需显示文摘文章简介虽然促癌蛋白YAP依赖性转录与肝癌发生发展密切相关,但YAP维持其促癌功能的分子机制尚未充分阐明。本研究证明TFCP2是YAP依赖性转录促肝癌效应的必需核因子。 | Xiao Zhang Fenyong Sun Yongxia Qiao Weisheng Zheng Ya Liu Yan Chen Qi Wu Xiangfan Liu Guoqing Zhu Yuxin Chen Yongchun Yu 潘秋辉 王佳谊 | 2018 | 科学新闻2018，0，4： | 0 |
| 8 | Protein interactions in the murine cytomegalovirus capsid revealed by cryoEM显示文摘Cytomegalovirus(CMV)is distinct among members of the Herpesviridae family for having the largest dsDNA genome(230 kb).Packaging of large dsDNA genome is known to give rise to a highly pressurized viral capsid,but molecular interactions conducive to the formation of CMV capsid resistant to pressurization have not been described.Here,we report a cryo electron microscopy(cryoEM)structure of the murine cytomegalovirus(MCMV)capsid at a 9.1Åresolution and describe the molecular interactions among the~3000 protein molecules in the MCMV capsid at the secondary structure level.Secondary structural elements are resolved to provide landmarks for correlating with results from sequence-based prediction and for structure-based homology modeling.The major capsid protein(MCP)upper domain(MCPud)containsα-helices andβ-sheets conserved with those in MCPud of herpes simplex virus type 1(HSV-1),with the largest differences identifi ed as a“saddle loop”region,located at the tip of MCPud and involved in interaction with the smallest capsid protein(SCP).Interactions among the bacteriophage HK97-like fl oor domain of MCP,the middle domain of MCP,the hook and clamp domains of the triplex proteins(hoop and clamp domains of TRI-1 and clamp domain of TRI-2)contribute to the formation of a mature capsid.These results offer a framework for understanding how cytomegalovirus uses various secondary structural elements of its capsid proteins to build a robust capsid for packaging its large dsDNA genome inside and for attach-ing unique functional tegument proteins outside. | Wong HHui Qiyi Tang Hongrong Liu Ivo Atanasov Fenyong Liu Hua Zhu ZHong Zhou | 2013 | Protein & Cell2013，4，11： | 0 |
| 9 | 3-D Marine CSEM Modeling in General Anisotropic Media by Using an Adaptive Finite Element Approach Based on the Vector-Scalar Potential显示文摘We present three-dimensional(3-D)modeling method of marine controlled-source electromagnetic(CSEM)fields in general anisotropic media using an adaptive finite element approach based on the vector-scalar potential.The modeling is based on the governing Helmholtz equations in the vector-scalar potential system.Unstructured tetrahedral grids are employed,which can exactly simulate the terrain relief and complex electrical structures.Moreover,based on the gradient recovery technology,the adaptive finite element approach is used to drive the mesh refinement,and make the finite element solutions converge gradually to the exact solutions.The primary-secondary field approach is used to improve the numerical accuracy of CSEM fields near the source point,where the primary field is calculated by using the quasi-analytical formula.The accuracy of this approach is verified by a one-dimensional model.Two 3-D models are used to demonstrate the effectiveness of the adaptive mesh refinement and the influences of dipping anisotropy layer on the marine CSEM responses for both inline and broadside geometries.The complex synthetic model is simulated to show the capability and flexibility of the approach for geometrically complex situations. | YE Yixin JIANG Fenyong FENG Zhibing LUO Ming LI Gang LI Yuguo | 2022 | Journal of Ocean University of China2022，21，5： | 0 |
| 10 | Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma显示文摘O-linked-β-N-acetylglucosamine(O-GlcNAc)glycosylation(O-GlcNAcylation)and phosphorylation are critical posttranslational modifications that are involved in regulating the functions of proteins involved in tumorigenesis and the development of various solid tumors.However,a detailed characterization of the patterns of these modifications at the peptide or protein level in hepatoblastoma(HB),a highly malignant primary hepatic tumor with an extremely low incidence in children,has not been performed.Here,we examined O-GlcNAc-modified or phospho-modified peptides and proteins in HB through quantitative proteomic analysis of HB tissues and paired normal liver tissues.Our results identified 114 O-GlcNAcylated peptides belonging to 78 proteins and 3494 phosphorylated peptides in 2088 proteins.Interestingly,41 proteins were modified by both O-GlcNAcylation and phosphorylation.These proteins are involved in multiple molecular and cellular processes,including chromatin remodeling,transcription,translation,transportation,and organelle organization.In addition,we verified the accuracy of the proteomics results and found a competitive inhibitory effect between O-GlcNAcylation and phosphorylation of HSPB1.Further,O-GlcNAcylation modification of HSPB1 promoted proliferation and enhanced the chemotherapeutic resistance of HB cell lines in vitro.Collectively,our research suggests that O-GlcNAc-modified and/or phospho-modified proteins may play a crucial role in the pathogenesis of HB. | Hang Song Ji Ma Zhixuan Bian Shuhua Chen Jiabei Zhu Jing Wang Nan Huang Minzhi Yin Fenyong Sun Min Xu Qiuhui Pan | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 0 |
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