|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Pericyte-like differentiation of human adipose-derived mesenchymal stem cells:An in vitro study显示文摘BACKGROUND Adipose-derived mesenchymal stem cells(ASCs)are characterized by long-term self-renewal and a high proliferation rate.Under adequate conditions,they may differentiate into cells belonging to mesodermal,endodermal or ectodermal lineages.Pericytes support endothelial cells and play an important role in stabilizing the vessel wall at the microcirculation level.The loss of pericytes,as occurs in diabetic retinopathy,results in a breakdown of the blood-retina barrier(BRB)and infiltration of inflammatory cells.In this context,the use of pericytelike differentiated ASCs may represent a valuable therapeutic strategy for restoring BRB damage.AIM To test in vitro strategies to obtain pericyte-like differentiation of human ASCs(hASCs).METHODS Different culture conditions were tested:hASCs cultured in a basal medium supplemented with transforming growth factorβ1;and hASCs cultured in a specific pericyte medium(PM-hASCs).In a further sample,pericyte growth supplement was omitted from the PM.In addition,cultures of human retinal pericytes(hRPCs)were used for comparison.Pericyte-like differentiation of hASCs was tested by immunocytochemical staining and western blotting to evaluate the expression ofα-smooth muscle actin(α-SMA)and neural/glial antigen 2(NG2).Interactions between human retinal endothelial cells(hRECs)and different groups of hASCs were investigated in co-culture experiments.In these cases,the expression of typical junctional proteins such as vascular endothelial-Cadherin,zonula occludens-1 and Occludin were assessed in hRECs.In an in vitro model of the BRB,values of trans-endothelial electrical resistance were measured when hRECs were co-cultured with various groups of pretreated hASCs.The values observed were compared with co-cultures of hRECs and hRPCs as well as with cultures of hRECs alone.Three-dimensional co-cultures of hRECs and hRPCs or pericyte-like hASCs in Matrigel were designed to assess their reciprocal localization.RESULTS After 3-6 d of culture,α-SMA and NG2 immunocytochemistry showed that the closest pericyte-like phenotype was observed when hASCs were cultured in Pericyte Medium(PM-hASCs).In particular,α-SMA immunoreactivity,already visible at the basal level in pericytes and ASCs,was strongly increased only when transforming growth factor was added to the culture medium.NG2 expression,almost undetectable in most conditions,was substantially increased only in PMhASCs.Immunocytochemical results were confirmed by western blot analysis.The presence of pericyte growth supplement seems to increase NG2 expression rather thanα-SMA,in agreement with its role in maintaining pericytes in the proliferative state.In co-culture experiments,immunoreactivity of vascular endothelial-Cadherin,zonula occludens-1 and Occludin was considerably increased in hRECs when hRPCs or PM-hASCs were also present.Supporting results were found by trans-endothelial electrical resistance measurements,gathered at 3 and 6 d of co-culture.The highest resistance values were obtained when hRECs were co-cultured with hRPCs or PM-hASCs.The pericyte-like phenotype of PM-hASCs was also confirmed in three-dimensional co-cultures in Matrigel,where PM-hASCs and hRPCs similarly localized around the tubular formations made by hRECs.CONCLUSION PM-hASCs seem able to strengthen the intercellular junctions between hRECs,likely reinforcing the BRB;thus,hASC-based therapeutic approaches may be developed to restore the integrity of retinal microcirculation. | Giuliana Mannino Florinda Gennuso Giovanni Giurdanella Federica Conti Filippo Drago Salvatore Salomone Debora Lo Furno Claudio Bucolo Rosario Giuffrida | 2020 | World Journal of Stem Cells2020，12，10： | 3 |
| 2 | Structural and functional differences of Litopenaeus vannamei crustins显示文摘 | Francisco V A Gloria Y P Florinda J V | 2004 | Comparative Bionehemistry and Physiology part B2004，38，4： | 1 |
| 3 | Structural and functional differences of Litopenaeus vannamei crustins显示文摘 | Francisco V A Angelica A V Gloria Y P Florinda J V | 2004 | Comp Bioehem Physiol (Part B Biochem Mol Bio)2004，138，4： | 1 |
| 4 | Increased myocardial apoptosis in patients with unfavorable left ventricular remodeling and early symptomatic post-infarction heart failure显示文摘 | Antonio Abbate Giuseppe G.L Biondi-Zoccai Rossana Bussani Aldo Dobrina Debora Camilot Florinda Feroce Raffaele Rossiello Feliciano Baldi Furio Silvestri Luigi M Biasucci Alfonso Baldi | 2003 | Journal of the American College of Cardiology2003，，5： | 1 |
| 5 | Evaluation ofsolubility and partition properties of ampicillin-based ionicliquids显示文摘 | Catarina Florinda Joao M Filipa Alves | 2013 | International Journal of Pharmaceutics2013，456，2： | 1 |
| 6 | Th17 and regulatory T lymphocytes in primary biliary cirrhosis and systemic sclerosis as models of autoimmune fibrotic diseases显示文摘 | Daniela Fenoglio Francesca Bernuzzi Florinda Battaglia Alessia Parodi Francesca Kalli Simone Negrini Raffaele De Palma Pietro Invernizzi Gilberto Filaci | 2012 | Autoimmunity Reviews2012，，2： | 1 |
| 7 | Alteration of Th17 and Treg cell subpopulations co-exist in patients affected with systemic sclerosis显示文摘 | Daniela Fenoglio Florinda Battaglia Alessia Parodi Silvia Stringara Simone Negrini Nicoletta Panico Marta Rizzi Francesca Kalli Giuseppina Conteduca Massimo Ghio Raffaele De Palma Francesco Indiveri Gilberto Filaci | 2011 | Clinical Immunology2011，，3： | 1 |
| 8 | Phenotypical and functional alterations of CD8 regulatory T cells in primary biliary cirrhosis显示文摘 | Francesca Bernuzzi Daniela Fenoglio Florinda Battaglia Marco Fravega M. Eric Gershwin Francesco Indiveri Aftab A. Ansari Mauro Podda Pietro Invernizzi Gilberto Filaci | 2010 | Journal of Autoimmunity2010，，3： | 1 |
| 9 | Chemical composition of the essential oil of commiphora erythraea 显示文摘 | Laura D M Vincenzo D F Florinda F | 2009 | Natural Product Communications2009，12，4： | 1 |
| 10 | Molecular characterization of bifunctional VHDL-CP from the hemolymph of white shrimp Penaeus vannamei 显示文摘 | Gloria Y P Florinda J V Mar a G R F | 2002 | Comparative Biochemistry and Physiology2002，132，： | 1 |
| 11 | Biochemical characteristics and biological properties of annurca apple cider显示文摘 | FLORINDA F ALFONSO D G ALFONSO S | 2012 | J Med Food2012，15，1： | 1 |
| 12 | Isolation of components with antimicrobial property from the donkey milk: A preliminary study显示文摘 | Filomena N Pierangelo O Florinda F | 2010 | The Open Food Sci J2010，4，： | 1 |
| 13 | Microencapsulation in food science and biotechnology显示文摘 | Filomena Nazzaro Pierangelo Orlando Florinda Fratianni Raffaele Coppola | 2011 | Current Opinion in Biotechnology2011，，2： | 1 |
| 14 | Mutagenic and antimutagenic properties of aqueous and ethanolic extracts from fresh and irradiated Tuber aestivum black truffle:a preliminary study显示文摘 | Fratianni Florinda Di Luccia Aldo | | 0，，： | 1 |
| 15 | A study of serum immunoglobulin levels in elderly persons that provides new insights into B cell immunosenescence显示文摘 | Florinda L Giuseppina C Maria MA | 2006 | Ann NY Acad Sci2006，1089，： | 1 |
| 16 | Microen- capsulation in Food Science and Biotechnology显示文摘 | FILOMENA N PIERANGELO O FLORINDA F | 2012 | Current Opinion in Biotechnology2012，23，： | 1 |
| 17 | Polyphenol composition and antioxidant activity of different grass pea (Lathyrus sativus), lentils (Lens culinaris), and chickpea (Cicer arietinum)ecotypes of the Campania region (Southern Italy)显示文摘 | FLORINDA F FEDERICA C AUTILIA C | 2014 | Journal of Functional Foods2014，7，2： | 1 |
| 18 | Mi- croencapsulation in Food Science and Biotechnology 显示文摘 | FILOMENA N PIERANGELO O FLORINDA F | 2012 | Current Opinion in Biotechnology2012，23，： | 1 |
| 19 | CagA antigen of helicobacter pylori and coronary instability: Insight from a clinico-pathological study and a meta-analysis of 4241 cases显示文摘 | Francesco Franceschi Giampaolo Niccoli Giuseppe Ferrante Antonio Gasbarrini Alfonso Baldi Marcello Candelli Florinda Feroce Nathalie Saulnier Micaela Conte Davide Roccarina Gaetano A. Lanza Giovanni Gasbarrini Silveri Nicolò Gentiloni Filippo Crea | 2008 | Atherosclerosis2008，，2： | 1 |
| 20 | Antimicrobial stewardship program in pediatric medicine显示文摘The rising threats from antimicrobial resistance due to inappropriate utilization of antimicrobial agents in health care including the pediatric population has been a topic of concern at the global level for the last several decades.The antimicrobial stewardship program(ASP)is a multidisciplinary institutional initiative focusing primarily on the improvement of antimicrobial prescribing practices and limiting inappropriate use.ASPs play an important role in the implementation of healthcare strategies in pediatrics worldwide to reduce antimicrobial resistance.Many published reports demonstrate how adapted ASPs in pediatrics result in improvement of unnecessary antimicrobial utilization,decreasing drug resistance and treatment failure,minimization of adverse clinical outcomes,decreasing healthcare costs and hospital length of stay,and optimization of diagnostic strategies.However,some barriers in pediatric ASP still exist.This narrative review describes core elements of ASP,the impact of implemented ASPs on pediatric healthcare,and challenges of pediatric ASP as seen by the authors. | Varvara Probst Florinda Islamovic Ayesha Mirza | 2021 | Pediatric Investigation2021，5，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员