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| 1 | Processing of advanced thermoelectric materials显示文摘Last two decades have witnessed significant progress in thermoelectric research,to which materials processing has crucial contributions.Compared with traditional zone-melting method used for fabricating bismuth telluride alloys,new powder-based processes have more freedom for manipulating nanostructures and nanocomposites.Thermoelectric performance enhancement is realized in most thermoelectric materials by introducing fine-grained and nano-composite structures with accurately controlled compositions.This review gives a comprehensive summary on the processing aspects of thermoelectric materials with three focuses on the powder synthesis,advanced sintering process and the formation of nano structures in bulk materials. | LI JingFeng PAN Yu WU ChaoFeng SUN FuHua WEI TianRan | 2017 | Science China(Technological Sciences)2017，60，9： | 13 |
| 2 | Oh Language Sense Training in College English Teaching Practice显示文摘The author supposes that there is a big gap between vocabulary and text in the course of second languageacquisition.Ignorance of individual sentence training to acquire language sense in the CET coursemight be one of the reasons to account for the failure of many students to be speakers of English afterthey graduate.Students should be encouraged to read and recite some model sentences and articles so asto gain a certain language sense as hardware and supporting materials in linguitic communication.Thus confidence is obtained and the gap between vocabulary and text can be easily jumped over. | Tang Fuhua Huizhou University,Guangdong | 1999 | Chinese Journal of Applied Linguistics1999，22，1： | 12 |
| 3 | Design of Photobioreactors for Mass Cultivation of Photosynthetic Organisms显示文摘 | Qingshan Huang Fuhua Jiang Lianzhou Wang Chao Yang | 2017 | Engineering2017，3，3： | 12 |
| 4 | Periodontitis may induce gut microbiota dysbiosis via salivary microbiota显示文摘The aim of this study was to identify whether periodontitis induces gut microbiota dysbiosis via invasion by salivary microbes.First,faecal and salivary samples were collected from periodontally healthy participants(PH group,n=16)and patients with severe periodontitis(SP group,n=21)and analysed by 16S ribosomal RNA sequencing.Significant differences were observed in both the faecal and salivary microbiota between the PH and SP groups.Notably,more saliva-sourced microbes were observed in the faecal samples of the SP group.Then,the remaining salivary microbes were transplanted into C57BL6/J mice(the C-PH group and the C-SP group),and it was found that the composition of the gut microbiota of the C-SP group was significantly different from that of the C-PH group,with Porphyromonadaceae and Fusobacterium being significantly enriched in the C-SP group.In the colon,the C-SP group showed significantly reduced crypt depth and zonula occludens-1 expression.The m RNA expression levels of pro-inflammatory cytokines,chemokines and tight junction proteins were significantly higher in the C-SP group.To further investigate whether salivary bacteria could persist in the intestine,the salivary microbiota was stained with carboxyfluorescein diacetate succinimidyl ester and transplanted into mice.We found that salivary microbes from both the PH group and the SP group could persist in the gut for at least 24 h.Thus,our data demonstrate that periodontitis may induce gut microbiota dysbiosis through the influx of salivary microbes. | Jun Bao Lili Li Yangheng Zhang Min Wang Faming Chen Shaohua Ge Bin Chen Fuhua Yan | 2022 | International Journal of Oral Science2022，14，3： | 9 |
| 5 | High temperature and high pressure rheological properties of high-density water-based drilling fluids for deep wells显示文摘To maintain tight control over rheological properties of high-density water-based drilling fluids, it is essential to understand the factors influencing the rheology of water-based drilling fluids. This paper examines temperature effects on the rheological properties of two types of high-density water-based drilling fluids (fresh water-based and brine-based) under high temperature and high pressure (HTHP) with a Fann 50SL rheometer. On the basis of the water-based drilling fluid systems formulated in laboratory, this paper mainly describes the influences of different types and concentration of clay, the content of a colloid stabilizer named GHJ-1 and fluid density on the rheological parameters such as viscosity and shear stress. In addition, the effects of aging temperature and aging time of the drilling fluid on these parameters were also examined. Clay content and proportions for different densities of brine-based fluids were recommended to effectively regulate the rheological properties. Four rheological models, the Bingham, power law, Casson and H-B models, were employed to fit the rheological parameters. It turns out that the H-B model was the best one to describe the rheological properties of the high-density drilling fluid under HTHP conditions and power law model produced the worst fit. In addition, a new mathematical model that describes the apparent viscosity as a function of temperature and pressure was established and has been applied on site. | Wang Fuhua Tan Xuechao Wang Ruihe Sun Mingbo Wang Li Liu Jianghua | 2012 | Petroleum Science2012，9，3： | 8 |
| 6 | Formation of coherent, core-shelled nano-particles in dilute Al-Sc-Zr alloys from the first-principles显示文摘We present a systematic first-principles based study on the formation of coherent L12-phase nanoparticles in dilute Al-Sc-Zr alloys.Bulk structures and properties,solute substitution,interface formation energies,and interfacial coherent strains are all calculated.Nucleation modeling and relevant energetic calculations are performed on various possible structures of L12-phase nano-precipitates,i.e.individual L12-Al3Sc and L12-Al3Zr,homogeneous L12-Al3(ScxZr1-x)and the core-shelled L12-Al3Sc(Al3Zr)and L12-Al3Zr(Al3Sc).The following insights are obtained.Matrix-dissolved Sc or Zr strongly prefers to substitute the X sublattice sites in L12-Al3X(X=Zr or Sc),while the inter-substitution between L12-Al3Sc and Al3Zr is only weakly feasible.The cube-on-cube orientation with the(100)/(100)contacting facets is the most energy favored for the Al/Al3Sc(L12),Al/Al3Zr(L12)and Al3Sc(L12)/Al3Zr(L12)interfaces.All these interfaces are highly coherent,with fairly low formation energy.And particularly,the Al3Sc(L12)/Al3Zr(L12)interface has essentially zero formation energy.Ternary L12-Al3(ScxZr1-x)precipitates tend to form a Al3Sc-based core and Al3Zr-based shell structure,with a relatively sharp inner interface of Al3Sc(L12)/Al3Zr(L12).This core-shelled structure becomes energetically more favorable for the particle size R>1–2 nm.The potential influence of the Sc/Zr ratio and temperature on the relative stabilities of L12-phases in Al is also evaluated and discussed. | Chaomin Zhang Yong Jiang Fuhua Cao Tao Hu Yiren Wang Dengfeng Yin | 2019 | Journal of Materials Science & Technology2019，35，5： | 8 |
| 7 | Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria显示文摘Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 ± 0.08, compared with 0.41 ± 0.09 of bacteria without VP28 (P <0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV. | SUN Yumiao LI Fuhua CHI Yanhong XIANG Jianhai | 2013 | Acta Oceanologica Sinica2013，32，2： | 7 |
| 8 | Differentiation of Hepatocellular Carcinoma from Hepatic Hemangioma and Focal Nodular Hyperplasia using Computed Tomographic Spectral Imaging显示文摘Background and Aims:Hepatocellular carcinoma(HCC)is the most common primary hepatic malignancy.This study was designed to investigate the value of computed tomography(CT)spectral imaging in differentiating HCC from hepatic hemangioma(HH)and focal nodular hyperplasia(FNH).Methods:This was a retrospective study of 51 patients who underwent spectral multiple-phase CT at 40–140 keV during the arterial phase(AP)and portal venous phase(PP).Slopes of the spectral curves,iodine density,water density derived from iodine-and water-based material decomposition images,iodine uptake ratio(IUR),normalized iodine concentration,and the ratio of iodine concentration in liver lesions between AP and PP were measured or calculated.Results:As energy level decreased,the CT values of HCC(n=31),HH(n=17),and FNH(n=7)increased in both AP and PP.There were significant differences in IUR in the AP,IUR in the PP,normalized iodine concentration in the AP,slope in the AP,and slope in the PP among HCC,HH,and FNH.The CT values in AP,IUR in the AP and PP,normalized iodine concentration in the AP,slope in the AP and PP had high sensitivity and specificity in differentiating HH and HCC from FNH.Quantitative CT spectral data had higher sensitivity and specificity than conventional qualitative CT image analysis during the combined phases.Conclusions:Mean CT values at low energy(40–90 keV)and quantitative analysis of CT spectral data(IUR in the AP)could be helpful in the differentiation of HCC,HH,and FNH. | Weixia Li Ruokun Li Xiangtian Zhao Xiaozhu Lin Yixing Yu Jing Zhang Kemin Chen Weimin Chai Fuhua Yan | 2021 | Journal of Clinical and Translational Hepatology2021，9，3： | 6 |
| 9 | Removing local irregularities of triangular meshes with highlight line models显示文摘The highlight line model is a powerful tool in assessing the quality of a surface. It increases the ffexibility of an interactive design environment. In this paper, a method to generate a highlight line model on an arbitrary triangular mesh is presented. Based on the highlight line model, a technique to remove local shape irregularities of a triangular mesh is then presented. The shape modification is done by solving a minimization problem and performing an iterative procedure. The new technique improves not only the shape quality of the mesh surface, but also the shape of the highlight line model. It provides an intuitive and yet suitable method for locally optimizing the shape of a triangular mesh. | YONG JunHai DENG BaiLin CHENG FuHua WANG Bin WU Kun GU HeJin | 2009 | Science in China(Series F)2009，52，3： | 5 |
| 10 | Human β-defensin 3 gene modification promotes the osteogenic differentiation of human periodontal ligament cells and bone repair in periodontitis显示文摘Efforts to control inflammation and achieve better tissue repair in the treatment of periodontitis have been ongoing for years.Humanβ-defensin 3,a broad-spectrum antimicrobial peptide has been proven to have a variety of biological functions in periodontitis;however,relatively few reports have addressed the effects of human periodontal ligament cells(h PDLCs)on osteogenic differentiation.In this study,we evaluated the osteogenic effects of h PDLCs with an adenoviral vector encoding humanβ-defensin 3 in an inflammatory microenvironment.Then humanβ-defensin 3 gene-modified rat periodontal ligament cells were transplanted into rats with experimental periodontitis to observe their effects on periodontal bone repair.We found that the humanβ-defensin 3 gene-modified h PDLCs presented with high levels of osteogenesis-related gene expression and calcium deposition.Furthermore,the p38 MAPK pathway was activated in this process.In vivo,humanβ-defensin 3 gene-transfected rat PDLCs promoted bone repair in SD rats with periodontitis,and the p38 mitogen-activated protein kinase(MAPK)pathway might also have been involved.These findings demonstrate that humanβ-defensin 3 accelerates osteogenesis and that humanβ-defensin 3 gene modification may offer a potential approach to promote bone repair in patients with periodontitis. | Lingjun Li Han Jiang Rixin Chen Jing Zhou Yin Xiao Yangheng Zhang Fuhua Yan | 2020 | International Journal of Oral Science2020，12，2： | 5 |
| 11 | Detection of hepatic and pancreatic fat infiltration in type II diabetes mellitus patients with IDEAL-Quant using 3.0T MR: comparison with single-voxel proton spectroscopy显示文摘 | Ma Jing Song Zhiqiang Yan Fuhua | 2014 | Chinese Medical Journal2014，，20： | 5 |
| 12 | Palynological evidence sheds new light on the age of the Liuqu Conglomerates in Tibet and its geological significance显示文摘The Liuqu Conglomerates consist of a suit of terrestrial molasse deposits formed in a foreland basin of the Himalaya-Tibet orogenic belt before the collision of India and Eurasia.These deposits record considerable geological information regarding the late tectonic evolution of the Neotethyan ocean.The palynological study of interlayers of mudstone and mud-sandstones indicates an Oligocene age.The palynological assemblage consists mainly of deciduous broad-leaved angiosperms,with some coniferous gymnosperms and evergreen broad-leaved angiosperms.The deposits reflect a broad-leaved deciduous forest or mixed with conifer-broad-leaved forest ecotypes,showing a warm-temperate or temperate zone climatic environment.This work provides significant new information about the tectonic evolution,paleogeography,and paleoenvironment of southern Tibet during the Cenozoic. | WEI LiJie LIU XiaoHan YAN FuHua MAI XueShun LI GuangWei LIU XiaoBing ZHOU XueJun | 2011 | Science China Earth Sciences2011，54，6： | 5 |
| 13 | Transcriptome-wide identification and expression profiling of Pinus massoniana MYB transcription factors responding to phosphorus deficiency显示文摘Myeloblastosis(MYB) proteins constitute one of the largest transcription factor(TF) families in plants and play crucial roles in regulating plant physiological and biochemical processes, including adaptation to diverse abiotic stresses. These TF families contain highly conserved MYB repeats(1 R, R2 R3, 3 R and 4 R) at the N-terminus. Roles for MYB TFs have been reported in response to such stresses as dehydration, salt, cold, and drought. The characterization of Masson pine(Pinus massoniana) MYB TFs are reported, including the analysis of MYB TFs expression in seedlings under controlled conditions and two different phosphate(Pi) deficient treatments. By searching for conserved MYB motifs in full transcriptomic RNA sequencing data for P. massoniana, 59 sequences were identified as MYB TFs. Conserved domainstructures and comparative functional and phylogenetic relationships of these MYB TFs with those in Arabidopsis were assessed using various bioinformatics tools. Based on microarray analysis, P. massoniana MYB genes exhibited different expression patterns under the two Pi deficiency conditions. Genes encoding MYB TFs that showed increased expression under critical Pi deficiency were identified, and some MYBs were differentially expressed only under conditions of severe Pi starvation. These results are useful for the functional characterization of MYB TFs that may be involved in the response to Pi deficiency and play divergent roles in plants. | Fuhua Fan Qingzhu Wang Xiaopeng Wen Guijie Ding | 2020 | Journal of Forestry Research2020，31，3： | 4 |
| 14 | MRI结合数字化钼靶诊断乳腺癌的临床研究(英文)显示文摘目的将在乳癌的察觉和诊断比较数字早期胸部肿瘤Ⅹ射线测定法和 MRI 的有效性并且象二的联合一样估计这些形式的价值。有外科和病理的 57 个病人证明了胸损害的方法(恶意, n = 32;良性, n = 46 ) 经历了数字早期胸部肿瘤Ⅹ射线测定法和 MRI,脉搏序列包括了 T 1 WI, T 2 WI,弥漫的加权的成像(DWI ) ,和在外科前的动态提高对比的 MRI。这二种形式的结果,所有损害根据 BI-RADS 分类被分类进 5 个组,并且成像调查结果被相关到组织病理学说。每种形式的敏感以及二的联合是计算的。这 78 乳房损害的结果,敏感为数字早期胸部肿瘤Ⅹ射线测定法是 78.13%%(25/32 ) 并且 93.75%(30/32 ) 为 MRI (P >
0.05 ) 。特性是 73.91%%(34/46 ) 并且 89.13%(41/46 ) 因此(P <
0.05 ) ,他们俩出现了统计差别。当这二种形式在联合被使用,敏感和特性分别地是 98.63% 和 97.16% 。在有 MRI 的联合的结论数字早期胸部肿瘤Ⅹ射线测定法在乳癌,敏感和特性的诊断是有用的当时,被提高与单个形式的相比。 | Fang Ye Mengsu Zeng Fuhua Yan Wei Feng Meiling Zhou Renchen Li Wenfang Chen | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，5： | 3 |
| 15 | Partial aortic root remodeling for root reconstruction in patients with acute type A dissection显示文摘In the present study,we reported our experience with partial aortic root remodeling for root reconstruction in patients with acute type A dissection,which involves in non-coronary sinus and/or the right coronary sinus with just one trimmed Dacron graft.Between February 2001 and May 2010,we performed partial aortic root remodeling in 40 patients,who underwent emergency surgical intervention.The dissected sinuses were excised leaving a3-5 mm rim of the aortic wall from the attached aortic valve cusps.A short piece(4-5 cm) of collagen coated woven polyester vascular prosthesis was trimmed with one or two 'tongues' to reconstruct the non-coronary sinus and/or the right coronary sinus,but without using separated patches.Additional procedures were including hemi-arch replacement in 11 patients,and total arch replacement plus stent-elephant trunk in 20 patients.The mean follow-up time was 36.4±3.6 months.In-hospital mortality was only 5.0%(2/40);furthermore,3(8.6%) patients underwent re-operation of the aortic valve and 2(5.7%) patients died during follow-up.At the end of follow-up,trivial or no aortic regurgitation was found in 33 patients,but mild aortic regurgitation was found in 2 patients.Our data suggest that the early and mid-term results of partial aortic root remodeling were favorable,and it restored valve durability and function.Thus,the use of technique for root reconstruction in patients with acute type A dissection should be vigorously encouraged. | Fuhua Huang Liangpeng Li Wei Qin Cunhua Su Liming Wang Liqiong Xiao Xin Chen | 2016 | The Journal of Biomedical Research2016，30，5： | 3 |
| 16 | Lead isotope geochemical characteristics of Pb-Zn-Cu deposits on the southwestern margin of Tarim,and their significance显示文摘The polymetallic(Pb,Zn,Cu,etc) ore belt on the southwestern margin of Tarim is one of the major regions with the greatest prospecting potential in Xinjiang.Reported in this paper are the lead isotope data for 66 sulfide samples(including 50 galena samples,15 chalcopyrite samples and 1 pyrite sample) collected from such representative deposits as Tamu,Tiekelike,Kalangu,Abalieke,etc.in this ore belt.The Pb isotopic ratios of 206 Pb/204 Pb,207 Pb/204 Pb and 208 Pb/204 Pb in the galena samples range from 17.931 to 18.176,15.609 to 15.818 and 38.197 to 38.944,with the average values of 18.017,15.684 and 38.462,respectively.Those in the chalcopyrite samples range from 17.926 to 18.144,15.598 to 15.628 and 38.171 to 38.583,with the average values of 18.020,15.606 and 38.262,respectively.The pyrite sample has the Pb isotopic ratios of 206 Pb/204 Pb,207 Pb/204 Pb and 208 Pb/204 Pb to be 17.980,15.604 and 38.145,respectively.In combination with the previous Pb isotope data for sulfides,it is found that there is only a slight variation in the Pb isotopic composition of galena,chalcopyrite,sphalerite and pyrite in the ore belt.However,there is some difference in Pb isotopic characteristics between galena and chalcopyrite,especially the Pb isotopic composition of galena shows an obvious linear correlation with some other relevant parameters(e.g.β and γ).The comprehensive analysis suggested that lead in galena(maybe including sphalerite and pyrite) was derived principally from wall rocks and underlying basement,and that in chalcopyrite only originated from the basement.The single-stage model ages of these sulfides couldn't indicate the time limit of metallogenesis(Pb,Zn,Cu,etc.),and the positive linear correlations for the Pb isotopic composition of galena are of no single-stage and two-stage Pb-Pb isochron significance.Furthermore,there are significant differences in Pb isotopic composition characteristics between the genetic type of deposits in this polymetallic ore belt and the Mississippi Valley type(MVT).In addition,the authors also pointed out that there is a phenomenon of differentiation(not paragenesis) for lead and copper elements during the process of metallogenesis in this ore belt. | SHEN Nengping ZHANG Zhengwei YOU Fuhua PENG Jiantang ZHU Xiaoqing XIAO Jiafei | 2012 | Chinese Journal Of Geochemistry2012，31，4： | 3 |
| 17 | Development of FGF-2-loaded electrospun waterborne polyurethane fibrous membranes for bone regeneration显示文摘Guided bone regeneration(G BR)membrane has been used to improve functional outcomes for periodontal regener ation.However,few studies have focused on the biomimetic membrane mim-icking the vascularization of the periodontal membrane.This study aimed to fabricate waterborne polyurethane(WPU)fibrous membranes loaJed fibroblast growth factor-2(FGF-2)via emulsion electrospinning,which can promote regeneration of periodontal tissue via the vascularization of the biomimetic GBR membrane.A biodegradable WPU was synthesized by using lysine and dime-thylpropionic acid as chain extenders according to the rule of green chemical synthesis technology.The WPU fibers with FGF-2 was fabricated via emulsion electrospinning.The results confirmed that controlled properties of the fibrous membrane had been achieved with controlled degradation,suitable mech anical properties and sustained release of the factor.The immunohistochemical expression of angiogenic-related factors was positive,meaning that FGF-2 loaded in fibers can sig-nificantly promote cell vasculariz ation.The fiber scaffold loaded FGF-2 has the potential to be used as a functional GBR membrane to promote the formation of extraosseous blood vessels during periodontal repairing. | Chi Zhang Jianxiong Wang Yujie Xie Li Wang Lishi Yang Jihua Yu Akira Miyamoto Fuhua Sun | 2021 | Regenerative Biomaterials2021，8，1： | 3 |
| 18 | Reconstruction of linkage maps in the distorted segregation populations of backcross, doubled haploid and recombinant inbred lines显示文摘Non-Mendelian segregation of markers, known as distorted segregation, is a common biological phenomenon. Although segregation distortion affects the estimation of map distances and the results of quantitative trait loci (QTL) mapping, the effects of distorted markers are often ignored in the construction of linkage maps and in QTL mapping. Recently, we have developed a multipoint method via a Hidden Markov chain method to reconstruct linkage maps in an F2 population that corrects for bias of map distances between distorted markers. In this article, the method is extended to cover backcross, doubled haploid and recombinant inbred line (RIL) populations. The results from simulated experiments show that: (1) the degree that two linked segregation distortion loci (SDL) affect the estimation of map distances increases as SDL heritability and interval length between adjacent markers increase, whereas sample size has little effect on the bias; (2) two linked SDL result in the underesti- mation of linkage distances for most cases, overestimation for an additive model with opposite additive effects, and unbiased estimation for an epistatic model with negative additive-by-additive effects; (3) the proposed method can obtain the unbiased estimation of linkage distance. This new method was applied to a rice RIL population with severely distorted segregation to reconstruct the linkage maps, and a bootstrap method was used to obtain 95% confidence intervals of map distances. The results from real data analysis further demonstrate the utility of our method, which provides a foundation for the inheritance analysis of quantitative and viability traits. | ZHU ChengSong WANG FuHua WANG JianFei LI GuangJun ZHANG HongSheng ZHANG YuanMing | 2007 | Chinese Science Bulletin2007，52，12： | 3 |
| 19 | Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis显示文摘A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fenneropenaeus chinensis by 3′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12.3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus (Litopenaeus) setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary electrophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen. | LIU Fengsong LI Fuhua XIANG Jianhai DONG Bo LIU Yichen ZHANG Xiaojun ZHANG Liusuo | 2008 | Acta Oceanologica Sinica2008，27，2： | 2 |
| 20 | Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity显示文摘Due to the insufficient long-term protection and significant efficacy reduction to new variants of current COVID-19 vaccines,the epidemic prevention and control are still challenging.Here,we employ a capsid and antigen structure engineering(CASE)strategy to manufacture an adenoassociated viral serotype 6-based vaccine(S663V-RBD),which expresses trimeric receptor binding domain(RBD)of spike protein fused with a biological adjuvant RS09.Impressively,the engineered S663V-RBD could rapidly induce a satisfactory RBD-specific IgG titer within 2 weeks and maintain the titer for more than 4 months.Compared to the licensed BBIBP-CorV(Sinopharm,China),a single-dose S663V-RBD induced more endurable and robust immune responses in mice and elicited superior neutralizing antibodies against three typical SARS-CoV-2 pseudoviruses including wild type,C.37(Lambda)and B.1.617.2(Delta).More interestingly,the intramuscular injection of S663V-RBD could overcome pre-existing immunity against the capsid.Given its effectiveness,the CASE-based S663VRBD may provide a new solution for the current and next pandemic. | Fuhua Wu Shuang Luo Yongshun Zhang Yangsen Ou Hairui Wang Zhaofei Guo Chunting He Shuting Bai Penghui He Min Jiang Xiaoyan Chen Guangsheng Du Xun Sun | 2023 | Acta Pharmaceutica Sinica B2023，13，5： | 2 |
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