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    题名 作者 年代 出处 被引量
1Subsurface Flow Processes in Sloping Cropland of Purple Soil显示文摘Subsurface flow is a prominent runoff process in sloping lands of purple soil in the upper Yangtze River basin.However,it remains difficult to identify and quantify.In this study,in situ runoff experimental plots were used to measure soil moisture dynamics using an array of time domain reflectometry(TDR) together with overland flow and subsurface flow using isolated collecting troughs.Frequency of preferential flow during rainfall events and the controls of subsurface flow processes were investigated through combined analysis of soil properties,topography,rainfall intensity,initial wetness,and tillage.Results showed that subsurface flow was ubiquitous in purple soil profiles due to welldeveloped macropores,especially in surface soils while frequency of preferential flow occurrence was very low(only 2 cases in plot C) during all 22 rainfall events.Dry antecedent moisture conditions promoted the occurrence of preferential flow.However,consecutive real-time monitoring of soil moisture at different depths and various slope positions implied the possible occurrence of multiple subsurface lateral flows during intensive storms.Rainfall intensity,tillage operation,and soil properties were recognized as main controls of subsurface flow in the study area,which allows the optimization of management practices for alleviating adverse environmental effects of subsurface flow in the region.TANG Jialilang ZHU Bo WANG Tao CHENG Xunqiang GAO Meirong LIN Henry 2012Journal of Mountain Science2012,9,1:7
2N_2O Emission from Paddy Field under Different Rice Planting Modes显示文摘Measurements of N2O emissions from conventional rice cultivation (CRC), CRC with straw mulching, system of rice intensification (SRI) and SRI with plastic film mulching were conducted through static chamber/gas-chromatography techniques. The results show that daily fluctuation of N2O emissions in jointing stage are much higher than in others. A type peak of N2O seasonal emission presented between jointing and bearing stages companying with high daily average temperature and low precipitation. Biomass and leaf stomatal conductance were observed. Total quantities of N2O emission were budgeted. The results showed that after jointing stage the average N2O emission flux of SRI with plastic film mulching increased significantly than CRC with straw mulching and SRI, the leaf stomatal conductance of those showed the same trend (p< 0.05 ). Yield and total quantity of N2O emission in CRC with straw mulching enhanced 13.7% and 10.7% compared with those of CRC, respectively. The total quantity of N2O emissions reduced 3.6% in SRI with plastic film mulching compared with CRC, however, the yield increase of that was not significant.WANG Yuying ZHU Bo WANG Yanqiang GAO Meirong MA Xiumei 2006Wuhan University Journal of Natural Sciences2006,11,4:7
3Size fraction of phytoplankton and the contribution of natural plankton to the carbon source of Zhikong scallop Chlamys farreri in mariculture ecosystem of the Sanggou Bay显示文摘The biomass and size fraction of phytoplankton in terms of chlorophyll a(Chl a) was measured during four cruises conducted in April, July, October 2013 and January 2014 in mariculture area, the Sanggou Bay, China.Results show that total Chl a levels in the surface seawater of the Sanggou Bay generally range from 0.10 to 20.46μg/L, with an average value of 2.13 μg/L. Nano-phytoplankton was the most important size-fraction and accounted for about 65.1% of total Chl a. In order to evaluate the importance of the 'protozoan trophic link' for energy transfer from the microbial loop to filter-feeding feeders, Zhikong scallop Chlamys farreri was then offered a natural planktonic community as potential prey. Results show that scallops obtained carbon source from natural plankton with the rate of 11 033.05 μg/(g·d). Protists(nanoflagellates and ciliates) were the dominant source of carbon retained by scallop(48.78%). The microbial loop provided 58.45% of the carbon source for farmed scallops. These results indicate that the microbial loop represent a valuable trophic resource in mariculture system of the Sanggou Bay.JIANG Zengjie DU Meirong FANG Jinghui GAO Yaping LI Jiaqi ZHAO Li FANG Jianguang 2017Acta Oceanologica Sinica2017,36,10:4
4THE APPLICATION OF THE ELECTRIC INFORMATION MEASURE ON HUMAN AURICULAR POINTS TO THE UPPER-DIGESTIVE-TRACT CANCER SURVEY显示文摘THEAPPLICATIONOFTHEELECTRICINFORMATIONMEASUREONHUMANAURICULARPOINTSTOTHEUPPER ̄DIGESTIVE-TRACTCANCERSURVEYZhuBing,LiNingshen,Y...Zhu Bing, Li Ningshen, Yu Zheng, Jia Meirong, Li Heshen(Department of Physics, Naming University, Naming,210008, China)Wang Yuexiang, Ding Jianhua, Gao Changming(Jiangsu Provincial institute of Prevention andTreatment of Tumour, Naming, 210008, China)Zhou 1994Chinese Journal of Biomedical Engineering(English Edition)1994,3,2:2
5Eff ect of polystyrene microplastics and temperature on growth,intestinal histology and immune responses of brine shrimp Artemia franciscana显示文摘Microplastics pollution and seawater temperature rise have been the major environmental issues,threatening the survival and biodiversity of marine organisms.This study evaluated the combined effect of temperature and polystyrene microplastics(MP)on Artemia,a filter-feeding crustacean that is widely used for environmental toxicology studies.Brine shrimp Artemia franciscana were exposed to three MP concentrations(0,0.2,and 2.0 mg/L)and three temperatures(22,26,and 30℃)for 14 d.In general,higher MP concentration and temperature led to a decreased survival rate and growth.Two-way ANOVA analysis indicated that the survival rate of Artemia was significantly impacted by both MP concentration and temperature(P<0.05),but there was no significant interaction between two factors(P>0.05).Growth of Artemia was significantly impacted by temperature(P<0.05),and with a significant interaction between two factors(P<0.05).Furthermore,the enzymatic activity,intestinal histological analyses,and immune gene expression were determined for Artemia reared at 30℃ with three MP concentrations(0,0.2,and 2.0 mg/L).The results showed that 2.0-mg/L MP resulted in reduced Artemia intestinal microvilli and exfoliated epithelia cells,significantly increased acid phosphatase(ACP)activity(P<0.05)and immunerelated gene ADRA1 B and CREB3 expression,revealing that higher MP concentration could induce oxidative and immunological stress on Artemia at 30℃.Overall,our study suggests that MP and temperature have combined adverse effect on Artemia,especially at relatively high temperature and polystyrene MP concentration.These findings are important to understand the potential ecological risks posed by these two factors on the organisms in marine environment.Xuekai HAN Yuyu ZHENG Chaoling DAI Hu DUAN Meirong GAO Md Rayhan ALI Liying SUI 2021Journal of Oceanology and Limnology2021,39,3:2
6Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition显示文摘Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.WANG Shanyue CUI Xueping XU Ruyi GAO Meirong SUI Liying 2019Journal of Oceanology and Limnology2019,37,5:2
7Assessing the effects of oyster/kelp weight ratio on water column properties: an experimental IMTA study at Sanggou Bay, China显示文摘Integrated Multi-Trophic Aquaculture(IMTA)is an effective method for sustainable aquaculture as species from different trophic levels could reduce negative effects from fed species in the environment.A proper proportion of different trophic species in an IMTA system could improve the aquaculture production and environmental sustainability.At present,research on the proper proportions for farming species is scarce.We investigated the effects of IMTA modes of oyster(Crassostrea gigas)and kelp(Saccharina japonica)in different weight ratios on water quality and carbonate system in a closed enclosure experiment for three days in the Sanggou Bay in Shandong Province,China,in December 2017.Nine collocation modes in oyster:kelp weight ratio were tested showing as 24:3,24:2,24:1,16:3,16:2,16:1,8:3,8:2,and 8:1.The water parameters were determined at 17:00 on Day 1(D1),and 6:00 and 17:00 on Days 2(D2)and 3(D3).As two-way ANOVA showed,all increased parameters(dissolved oxygen(DO),pH,chl a,the carbonate system and pCO2)were significantly related to oyster-kelp modes,and interaction between modes and time were also significant(P<0.05).On the 3th day,the 8:3 mode was the highest in DO,pH,chl a,CO32-(P<0.05),and dissolved inorganic carbon(DIC),HCO3-,CO2,and pCO2 were the lowest(P<0.05).According to previous references and the results of this study,the appropriate oyster:kelp proportion at the beginning of winter is from 8:2 to 8:3.The results of this study may help government to optimize the aquaculture structure of Sanggou Bay.FANG Jinghui FANG Jianguang CHEN Qionglin MAO Yuze JIANG Zengjie DU Meirong GAO Yaping LIN Fan 2020Journal of Oceanology and Limnology2020,38,6:2
8Constructing a biomimetic robust bi-layered hydrophilic lubrication coating on surface of silicone elastomer显示文摘Silicone elastomers-based materials have been extensively involved in the field of biomedical devices,while their use is extremely restricted due to the poor surface lubricity and inherent hydrophobicity.This paper describes a novel strategy for generating a robust layered soft matter lubrication coating on the surface of the polydimethylsiloxane(PDMS)silicone elastomer,by entangling thick polyzwitterionic polyelectrolyte brush of poly(sulfobetaine methacrylate)(PSBMA)into the sub-surface of the initiator-embedded stiff hydrogel coating layer of P(AAm-co-AA-co-HEMA-Br)/Fe,to achieve a unified low friction and high load-bearing properties.Meanwhile,the stiff hydrogel layer with controllable thickness is covalently anchored on the surface of PDMS by adding iron powder to provide catalytic sites through surface catalytically initiated radical polymerization(SCIRP)method and provides high load-bearing capacity,while the topmost brush/hydrogel composite layer is highly effective for aqueous lubrication.Their synergy effects are capable of attaining low friction coefficient(COFs)under wide range of loaded condition in water environment with steel ball as sliding pair.Furthermore,the influence of mechanical modulus of the stiff hydrogel layer on the lubrication performance of layered coating is investigated,for which the COF is the lowest only when the modulus of the stiff hydrogel layer well matches the PDMS substrate.Surprisingly,the COF of the modified PDMS could remain low friction(COF<0.05)stably after encountering 50,000 sliding cycles under 10 N load.Finally,the surface wear characterizations prove the robustness of the layered lubricating coating.This work provides a new route for engineering lubricious silicon elastomer with low friction,high load-bearing capacity,and considerable durability.Luyao GAO Xiaoduo ZHAO Shuanhong MA Zhengfeng MA Meirong CAI Yong-Min LIANG Feng ZHOU 2022Friction2022,10,7:2
9Feasibility Study of Aluminum Hydroxide Sol as Adjuvant to Prepare Stable Vaccine Using Newcastle Disease Virus as a Model显示文摘Aluminum hydroxide sol (consisting of aluminum hydroxide nano-particles) prepared by a hydrothermal method was used as nano-aluminum adjuvant adsorbed the inactived Newcastle disease virus (NDV). The zeta potential of aluminum hydroxide colloidal particles,the inactivated NDV and the nano-aluminum adjuvant NDV are +48.16 mV,?13.8 mV and +39.97 mV,respec-tively. The nano-aluminum adjuvant vaccine was transparent and stable without precipitate,whereas the conventional aluminum ad-juvant vaccine was turbid and a white precipitate was visible soon. The hemagglutination inhibition (HI) titers of the nano-aluminum adjuvant group were generally higher than those of the conventional aluminum group except the titer on day 29. The results show that the nano-aluminum adjuvant has better stability and higher levels of antibodies for a longer time than the conventional aluminum adjuvant.SONG Meirong LI Xinsheng GAO Wenming FANG Shaoming CUI Baoan 2011Wuhan University Journal of Natural Sciences2011,16,1:1
10Angiotensin1-7 protects cardiomyocytes from hypoxia/reoxygenation-induced oxidative stress by preventing ROS-associated mitochondrial dysfunction and activating the Akt signaling pathway显示文摘Pengjun Zhao Fen Li Wei Gao Jing Wang Lijun Fu Yiwei Chen Meirong Huang 2015Acta Histochemica2015,,8:1
11Fibers reinforced composite hydrogels with improved lubrication and load-bearing capacity显示文摘Hydrogels as one kind of soft materials with a typical three-dimensional(3D)hydrophilic network have been getting great attention in the field of biolubrication.However,traditional hydrogels commonly show poor tribology performance under high-load conditions because of their poor mechanical strength and toughness.Herein,pure chemical-crosslinking hydrogels mixed with different types of the micron-scale fibers can meet the requirements of strength and toughness for biolubrication materials,meanwhile the corresponding tribology performance improves significantly.In a typical case,three kinds of reinforcement matrix including needle-punched fibers,alginate fibers,and cottons are separately combined with Poly(n-vinyl pyrrolidone)-poly 2-hydroxyethyl methacrylate(PVP-PHEMA)hydrogels to prepare fibers reinforced composite hydrogels.The experimental results show that the mechanical properties of fibers reinforced composite hydrogels improve greatly comparable with pure PVP-PHEMA hydrogels.Among three kinds of fibers reinforced composite hydrogel,the as-prepared composite hydrogels reinforced with needle-punched fibers possess the best strength,modulus,and anti-tearing properties.Friction tests indicate that the fibers reinforced composite hydrogels demonstrate stable water-lubrication performance comparable with pure PVP-PHEMA hydrogels.Besides,the hydrogel-spunlace fiber samples show the best load-bearing and anti-wear capacities.The improved tribology performance of the composite hydrogels is highly related to mechanical property and the interaction between the fibers and hydrogel network.Finally,spunlace fibers reinforced hydrogel materials with high load-bearing and low friction properties are expected to be used as novel biomimetic lubrication materials.Jiawei LI Luyao GAO Rongnian XU Shuanhong MA Zhengfeng MA Yanhua LIU Yang WU Libang FENG Meirong CAI Feng ZHOU 2022Friction2022,10,1:1
12Identifying the Zn-Co binary as a robust bifunctional electrocatalyst in oxygen reduction and evolution reactions via shifting the apexes of the volcano plot显示文摘The performance of an electrocatalyst is closely correlated with the binding strength of key oxygencontaining intermediates,i.e.,*OOH,*O and*OH,in the oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).Facile strategies to achieve favorable binding strength of these oxygen-containing species are urgently demanded,yet it still remains great challenges.Herein,the Zn-Co bimetallic isolation,which serves as an ideal model,is studied systematically by the density functional theory(DFT).Reaction activity volcano plots are built from 48 models,among them the ZnCoN6-gra(I)configuration is confirmed to be the most stable,featured of the strongest interaction with the oxygen-containing species.Optimal △G*O(free energy change of an atomic oxygen containing intermediate)is facilitated,which effectively drifts the volcano peaks of ORR and OER closer to each other,enabling promising bifunctional catalyst.Moreover,the small overpotential in the simulation of protonation and oxidation by hydroxy groups rationalizes the durability of the catalyst in both acid and alkaline media.Jing Wang Rui Xu Yuling Sun Qian Liu Meirong Xia Yan Li Faming Gao Yufeng Zhao John S.Tse 2021Journal of Energy Chemistry2021,30,4:1
13Expansins and coleoptile elongation in wheat显示文摘Qiang Gao Meirong Zhao Feng Li Qifang Guo Shichao Xing Wei Wang 2008Protoplasma (-)2008,,1:1
14Stormwater Runoff Pollution in a Rural Township in the Hilly Area of the Central Sichuan Basin,China显示文摘Stormwater runoff in rural townships has a high potential for water quality impairment but little information is available on strormwater runoff pollution from rural townships.To investigate the characteristics of runoff pollution in a rural township,a catchment(2.32 ha) in Linshan Township,Sichuan,China was selected to examine runoff and quality parameters including precipitation,flow rate,and total nitrogen(TN),dissolved nitrogen(DN),total phosphorus(TP),dissolved phosphorus(DP),particulate phosphorus(PP),chemical oxygen demand(COD) and suspended solid(SS) in 12 rainfall events occurring between June 2006 and July 2007.Results show that the annual pollutant loads were 47.17 kg ha-1 for TN,6.64 kg ha-1 for TP,1186 kg ha-1 for COD,and 4297 kg ha-1 for SS.DN and PP were the main forms of nitrogen and phosphorus in stormwater runoff.TP,COD and SS showed medium mass first flushes,in which nearly 40% of the total pollutant masses were transported by the first 30% of total flow volume.The peak of pollutant concentration appeared before the peak of runoff due to the first flush of accumulative pollutants in impervious areas and drainage ditches.The EMC values of TN,TP,DN and PP were negatively correlated to the maximum rainfall intensity,precipitation,total flow volume,and runoff duration(P<0.05,n=12),while EMC of COD and SS were not related to any rainfall characteristics.The FF30(FF,First Flush) for TN,TP,COD and SS were positively correlated to the maximum rainfall intensity(P<0.05,n=12),and TP was also positively correlated to the average rainfall intensity(P<0.05,n=12),indicating that the magnitude of first flush increased with the rainfall intensity in the Linshan Township.LUO Zhuanxi WANG Tao GAO Meirong TANG Jialiang ZHU Bo 2012Journal of Mountain Science2012,9,1:1
15Applicability of Perinereis aibuhitensis Grube for Fish Waste Removal from Fish Cages in Sanggou Bay,P.R.China显示文摘The present study investigated the applicability of integrated polychaete-fish culture for fish waste removal to offset negative impact induced by organic benthic enrichment.A field study demonstrated that deposition rate was significantly higher underneath the fish farm than that in control area.The material settling under the farm was characterized by a high amount of fish feces(45%) and uneaten feed(27%).Both feeding rate(FR) and apparent digestibility rate(ADR) increased with decreasing body weight,as was indicated by significantly a higher rate observed for the groups containing smaller individuals in a lab study.The nutrient in fresh deposited material(De) was higher than that in sediments collected under the farm(Se),resulting in lower feces production but higher apparent digestibility rate for the De group as feeding rate was similar.Consequently,higher nutrient removal efficiency was observed in the De group.A mass balance approach indicated that approximately 400–500 individuals m^(-2) is required for removing all waste materials deposited underneath the fish farm,whereas abundance can be lower(about 300–350 individuals m^(-2)) when only the fish waste needs to be removed.The results showed that a significant amount of waste had been accumulated in the fish cages in Sanggou Bay.The integration of fish with P.aibuhitensis seems promising for preventing organic pollution in the sediment and therefore is an effective strategy for mitigating negative effect of fish farms.Thus such integration can become a new IMTA(integrated multi-trophic aquaculture) model in Sanggou Bay.FANG Jinghui JIANG Zengjie JANSEN Henrice M. HU Fawen FANG Jianguang LIU Yi GAO Yaping DU Meirong 2017Journal of Ocean University of China2017,16,2:1
16Response of Yesso scallop Patinopecten yessoensis to acute temperature challenge: physiological and biochemical parameters显示文摘Water temperature is generally considered to be a major factor af fecting the physiological and biochemical activities of marine bivalves. Here, the physiological and biochemical responses of Yesso scallop, Patinopecten yessoensis, to acute water temperature changes in summer were studied. Scallops were transferred directly to a lower temperature( T dec treatment)(from 23°C to 15°C) or to a higher temperature( Ti nc treatment)(from 15°C to 23°C) for 72 h, respectively. Results showed that the oxygen consumption and ammonia-N excretion rates of P. yessoensis decreased signi?cantly in the T dec treatment but increased dramatically at 6 h in the Ti nc treatment( P <0.05). In the T dec treatment, hepatopancreas antioxidant enzyme activities, superoxide dismutase(SOD) and catalase(CAT) activities, increased substantially within 72 h( P <0.05). However, a signi?cant decrease in CAT activity was found at 12 h in the Ti nc treatment( P <0.01).A signi?cant enhancement of acid phosphatase(ACP) activity and malondialdehyde(MDA) content was detected when scallops were acutely exposed to a temperature of 15°C. The levels of Cu/Zn-SOD gene expression in their gills up-regulated signi?cantly in response to acute temperature changes( P <0.01). These data suggest that acute temperature change af fects physiological and biochemical functions, and improve our knowledge of P. yessoensis under conditions of thermal stress.JIANG Weiwei DU Meirong FANG Jianguang GAO Yaping MAO Yuze CHEN Qionglin LIN Fan JIANG Zengjie 2019Journal of Oceanology and Limnology2019,37,1:1
17Diversity and connectedness of brine shrimp viruses in global hypersaline ecosystems显示文摘Brine shrimp(Artemia)has existed on Earth for 400 million years and has major ecological importance in hypersaline ecosystems.As a crucial live food in aquaculture,brine shrimp cysts have become one of the most important aquatic products traded worldwide.However,our understanding of the biodiversity,prevalence and global connectedness of viruses in brine shrimp is still very limited.A total of 143 batches of brine shrimp(belonging to seven species)cysts were collected from six continents including 21 countries and more than 100 geographic locations worldwide during 1977–2019.In total,55 novel RNA viruses were identified,which could be assigned to 18 different viral families and related clades.Eleven viruses were dsRNA viruses,16 were+ssRNA viruses,and 28 were−ssRNA viruses.Phylogenetic analyses of the RNA-directed RNA polymerase(RdRp)showed that brine shrimp viruses were often grouped with viruses isolated from other invertebrates and fungi.Remarkably,most brine shrimp viruses were related to those from different hosts that might feed on brine shrimp or share the same ecological niche.A notable case was the novel brine shrimp noda-like virus 3,which shared 79.25%(RdRp)and 63.88%(capsid proteins)amino acid identity with covert mortality nodavirus(CMNV)that may cause losses in aquaculture.In addition,both virome composition and phylogenetic analyses revealed global connectedness in certain brine shrimp viruses,particularly among Asia and Northern America.This highlights the incredible species diversity of viruses in these ancient species and provides essential data for the prevalence of RNA viruses in the global aquaculture industry.More broadly,these findings provide novel insights into the previously unrecognized RNA virosphere in hypersaline ecosystems worldwide and demonstrate that human activity might have driven the global connectedness of brine shrimp viruses.Xuan Dong Cixiu Li Yiting Wang Tao Hu Fan Zhang Fanzeng Meng Meirong Gao Xuekai Han Guohao Wang Jiahao Qin Hans Nauwynck Edward CHolmes Patrick Sorgeloos Liying Sui Jie Huang Weifeng Shi 2024Science China(Life Sciences)2024,67,1:0
18Correction: Characterization of a small-molecule inhibitor targeting NEMO/IKKβ to suppress colorectal lcancergrowth显示文摘In the process of collating the raw data,the authors noticed an inadvertent mistake occurred in Fig.3b that needs to be corrected after online publication of the article.In Fig.3b,as a result of an error in the graphics panel arrangement process,the band of NEMO was repeatedly inserted asβ-actin by mistake.The correct band is shown as below and in the updated Fig.3b.The correction did not affect any of our results or discussion as present in the original publication.We regret any inconvenience this has caused.Zhenlong Yu Jian Gao Xiaolei Zhang Yulin Peng Wenlong Wei Jianrong Xu Zhenwei Li Chao Wang Meirong Zhou Xiangge Tian Lei Feng Xiaokui Huo Min Liu Mingliang Ye De-an Guo Xiaochi Ma 2023Signal Transduction and Targeted Therapy2023,8,10:0
19外源酶添加对人工林凋落物质量残留、养分含量和微生物群落的影响显示文摘凋落物输入对森林土壤性质和生态系统功能有很大影响。凋落物快速分解可减少人工林农业废弃物,促进养分循环。酶是凋落物分解和元素释放的关键角色。然而,有关外源酶制剂对凋落物分解的影响研究却很少。为了探究外源酶如何影响凋落物质量残留率、营养元素释放(C,N,K)和微生物群落多样性,本研究以人工林枇杷(Eriobotrya japonica)凋落叶为研究对象,将5种外源酶(漆酶、木质素过氧化物酶、亮氨酸氨基肽酶、纤维素酶、酸性磷酸酶)施加于凋落物表面后恒温恒湿分解189天。在分解过程中,测定凋落物质量残留率和主要元素含量,分解结束后测定凋落物表面附着的微生物多样性。研究结果显示,漆酶和木质素过氧化物酶通过影响微生物多样性、氮和钾的含量来促进凋落物的分解。亮氨酸氨基肽酶促进了氮元素的富集,从而降低凋落物质量。但纤维素酶、漆酶和木质素过氧化物酶的施加对凋落物中纤维素和木质素的降解没有影响。这些研究结果证明,外源酶施加可改变凋落物的养分含量和微生物群落,从而影响凋落物的分解。综上,为了有效调控农业废弃物凋落物的分解速率,外源酶对凋落物分解的影响是未来的重点研究方向之一。Huayue Nie Chenrui Wang Meirong Tian Jixi Gao 2023Journal of Plant Ecology2023,16,6:0
20Halomonas-PHB protects gnotobiotic Artemia against Vibrio and m odifies Artemia gut microbiota in xenic culture conditions显示文摘The prokaryotic cell storage compound ploy-β-hydroxybutyrate(PHB)has been considered as prebiotics that can be applied in aquaculture.In this paper,the dietary effect of a PHB-accumulating Halomonas strain(HM·PHB)identified from our previous work were studied in Artemia under gnotobiotic and xenic culture conditions,in comparison of Halomonas without PHB accumulation(HM)and microalgae Isochrysis(ISO)feeding.Under gnotobiotic condition,both HM·PHB and HM served as sole food supporting Artemia survival.Although both HM·PHB and HM feeding had no significant difference on Artemia survival percentage(P>0.05),HM·PHB significantly improved their resistance against Vibrio anguillarum challenge(P<0.05).Mass Artemia culture were further performed in xenic condition.Compared to ISO,HM·PHB feeding protected Artemia against V.anguillarum challenge(P<0.05),and HM·PHB and HM feeding resulted in increased T-AOC,pepsin,T-SOD and CAT activities(P<0.05).High throughput sequencing analysis showed that HM·PHB and HM feeding resulted in a lower Artemia gut microbial diversity(P<0.05),and modified the gut microbial community by remarkably reducing the Vibrio proportion.The outcome of the paper confirmed the beneficial effect of Halomonas-PHB in Artemia culture,which supports the use of Halomonas-PHB in the production of bio-secured live feed Artemia.Liying SUI Xiaocui LIU Namin PAN Xue LIU Meirong GAO 2023Journal of Oceanology and Limnology2023,41,4:0
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