|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Human CMTM2/CKLFSF2 enhances the ligand-induced transactivation of the androgen receptor显示文摘CKLF (chemokine-like factor)-like MARVEL (MAL and related proteins for vesicle trafficking and mem-brane link domain) transmembrane domain containing (CMTM) is a novel gene family. One member of this family, CMTM2, also named chemokine-like factor superfamily 2 (CKLFSF2), is expressed highly in the testis and moderately in the prostate, marrow and peripheral blood cells. However, the function of human CMTM2 remains unknown. Here, we found that CMTM2 was upregulated in 5α-dihydrotestos- terone (DHT)-treated LNCaP cells. We investigated the relationship between CMTM2 and the androgen receptor. Our results showed that CMTM2 enhanced DHT-mediated androgen receptor (AR) transacti-vation and the expression of prostate specific antigen (PSA). We also observed that CMTM2 enhanced the AR protein level, which was reversed by silencing endogenous CMTM2 expression, which sug-gested that CMTM2 might play an important role in maintaining the AR protein level. We also found that CMTM2 suppressed Akt activation. A previous study showed that Akt could phosphorylate AR at Ser210 and Ser790 and lead to AR ubiquitylation and degradation as well as suppression of AR activity. Taken together, suppressing Akt activation and increasing the AR protein level might be one of the mechanisms for the CMTM2-mediated enhancement of AR transactivation. | LIU DaZhen YIN CaiHua ZHANG YingMei TIAN LinJie LI Ting LI Dan MA DaLong GUO YingLu WANG Ying | 2009 | Chinese Science Bulletin2009，54，6： | 7 |
| 2 | Interaction of hTCF4 by yeast two-hybrid system显示文摘To investigate the interaction of hTCF4, the yeast two-hybrid system has been used for testing the interaction of mutants of hTCF4 with themselves. Mutants of hTCF4 (hTCF4 Ⅰ ) and hTCF4 Ⅱ) have been obtained by polymerase chain reaction (PCR). Bait (hTCF4 Ⅰ -pDBLeu and hTCF4Ⅱ-pDBLeu) and prey (hTCF4 Ⅰ -pPC86 and hTCF4Ⅱ-pPC86) have been constructed by DNA recombination for yeast two-hybrid. Interaction of hTCF4Ⅱ with itself is found in the reverse yeast two-hybrid system (GIBCOBRL Co.). However, no interactions are found in hTCF4Ⅱ with hTCF4 Ⅰ and in hTCF4 Ⅰ with hTCF4 Ⅰ . These results suggest that hTCF4 could interact with itself to form homodimer or homocopolymer and perform the transcriptional activating function through LZ or HLH motifs in nucleus of renal cell carcinoma. | LIN Guiting ZHANG Zhiwen ZANG long XIN Dianqi CHANG Zhijie GUO Yinglu | 2000 | Chinese Science Bulletin2000，45，21： | 1 |
| 3 | Aberrant activity of Wnt/Frizzled signaling pathway in renal cancer cell lines显示文摘The expression of Wnt, Wnt receptor-Frizzled, and several other key components in Wnt pathway in renal cancer cell lines was studied. The result of semi-quantitative RT-PCR has shown that the expression level of Wnt5A and hFz5 mRNA were higher in renal cancer cell line (GRC-1) than in normal renal cell line (HK-2). This result has been confirmed by in situ hybridization. The expression of p-catenin protein was obviously higher in GRC-1 than in HK-2 (P<0.01), but there were no different expressions of its mRNA between 3 lines. The reasons of the overexpression of β-catenin has been investigated by means of immunocytochemistry, SSCP and so on, no mutation of β-catenin gene and APC were found. That means that the overexpression of Wnt5A/hFz5 might be the reason of overexpression of β-catenin. It was concluded that the aberrant activity of Wnt pathway might play an important role in renal cell carcinoma. | ZANG Tong ZHUANG Liyan ZHANG Zhiwen XIN Dianqi GUO Yinglu | 2000 | Chinese Science Bulletin2000，45，18： | 1 |
| 4 | Mitochondrially localized EGFR is independent of its endocytosis and associates with cell viability显示文摘位于表皮的生长下面的分子的机制在线粒体的因素受体(EGFR ) 本地化仍然保持大部分未知。用有免疫力的电子显微镜学,我们验证了那 EGFR 能在也上局部性外部或线粒体的内部膜。变异的受体缺乏的氨基酸 646660 在移植是有缺陷的到细胞器上,而有有缺点的 endocytosis 的变异的受体显示出在表皮的生长因素(EGF ) 的刺激之上移动到线粒体上的一个更大的能力。Gefitinib, EGFR kinase 的一个禁止者,在还,处理的短时间仅仅在暴露的更长的时间以后减少了房间生存能力以及 mitochondrial EGFR 的数量以后,禁止了受体 endocytosis。而且,当房间暴露于 apoptotic inducer etoposide 时, mitochondrial EGFR 转移的内容被减少。通常在 mitochondrial EGFR 与衰落与一致的导致 EGF 的规划房间死亡。这些数据显示 mitochondrial 局部性的 EGFR 独立于它的成为主观并且可以与房间幸存被相关并且参予导致 ligand 的规划房间死亡。 | Yuan Yao Gang Wang Zhe Li Bing Yan Yinglu Guo Xuejun Jiang Zhijun Xi | 2010 | Acta Biochimica et Biophysica Sinica2010，42，11： | 1 |
| 5 | PCAF/GCN5介导的RPA1乙酰化促进核苷酸切除修复显示文摘文章简介本研究聚焦于DNA损伤修复。复制蛋白A(RPA,Replication protein A)是一类重要的单链DNA结合蛋白复合物,主要参与DNA的复制和修复。RPA复合物包含三个亚基,分别是RPA1、RPA2、RPA3。 | Meimei Zhao Rui Geng Xiang Guo Ruoshi Yuan Xiao Zhou Yanyan Zhong Yanfei Huo Mei Zhou Qinjian Shen Yinglu Li Weiguo Zhu 王嘉东 | 2018 | 科学新闻2018，0，4： | 0 |
| 6 | Correlation between the gut microbiome and neurodegenerative diseases:a review of metagenomics evidence显示文摘A growing body of evidence suggests that the gut microbiota contributes to the development of neurodegenerative diseases via the microbiota-gut-brain axis.As a contributing factor,microbiota dysbiosis always occurs in pathological changes of neurodegenerative diseases,such as Alzheimer’s disease,Parkinson’s disease,and amyotrophic lateral sclerosis.High-throughput sequencing technology has helped to reveal that the bidirectional communication between the central nervous system and the enteric nervous system is facilitated by the microbiota’s diverse microorganisms,and for both neuroimmune and neuroendocrine systems.Here,we summarize the bioinformatics analysis and wet-biology validation for the gut metagenomics in neurodegenerative diseases,with an emphasis on multi-omics studies and the gut virome.The pathogen-associated signaling biomarkers for identifying brain disorders and potential therapeutic targets are also elucidated.Finally,we discuss the role of diet,prebiotics,probiotics,postbiotics and exercise interventions in remodeling the microbiome and reducing the symptoms of neurodegenerative diseases. | Xiaoyan Liu Yi Liu Junlin Liu Hantao Zhang Chaofan Shan Yinglu Guo Xun Gong Mengmeng Cui Xiubin Li Min Tang | 2024 | Neural Regeneration Research2024，19，4： | 0 |
| 7 | Thoracic interstitial injection of drug-liposomes in mice for treating atherosclerosis显示文摘Intervaginal space injection(ISI)is a novel mode of administration investigated over the last decade.After injecting nanoparticles into the intervaginal space,they can be transported along low flow resistance channels into the interstitial space.This transport has a certain delivery direction,and site-specific injection can work on specific organs or tissues.In this study,the thorax,a new ISI site in the interstitial surrounding the internal thoracic artery named the thoracic interstitial injection(tISI)was investigated.To prove the targeting ability of the tISI,two sizes of gold nanoparticles(AuNPs)(47 and 87 nm)were administered to mice.After 1 h,the biodistribution of AuNPs in the tissues was measured via single particle inductively coupled plasma mass spectrometry(spICP-MS).The results showed that the concentration of AuNPs in the aorta after tISI injection was significantly higher than that after intravenous injection.Moreover,fewer nanoparticles with larger particle sizes were observed to have entered the blood and were better targeted to the aorta.Thereafter,tanshinone IIa sodium sulfonate liposomes were administered for the treatment of aortic atherosclerosis.The proportion of aortic plaques in atherosclerotic Apoe-/-mice administered via tISI was significantly lower than that in other model animals(P<0.001).Furthermore,the proteoglycan content and CD68-positive cell count in the plaques were significantly reduced.The vascular elastic fibers at the plaque site were thickened,and fractures were reduced.tISI was,therefore,determined to be an effective strategy for the treatment of atherosclerotic aortic plaques. | Tun Yan Huizhen Sun Yahong Shi Ya Gao Xi Lu Kai Li Yuting Zhu Qiang Zhang Tingting Li Zhongxian Li Xiaohan Zhou Yuting Guo Yinglu Ji Xiaoli Shi Dong Han | 2023 | Nano Research2023，16，4： | 0 |
| 8 | Congratulations to UroPrecision:Embark on a remarkable journey显示文摘Dear Editors,I would like to express my sincere congratulations to you on the first issue of the new journal—UroPrecision.As a urologist who dedicated most of my life to clinical and basic urology for decades of years,I am immensely proud to witness the launch of a journal that is dedicated to speeding up the growth and integration of precision medicine principles in the field of urology as well as building up the influence of important research results in the international community. | Yinglu Guo | 2023 | UroPrecision2023，1，1： | 0 |
| 9 | Census report on Chinese urological surgeons显示文摘Objective:To grasp the general situation of Chinese urological surgeons and the status quo of their scientific research,work and training,thus providing valuable recommendations for urological talent team construction in future.Methods:The survey respondentswere the urological surgeons,who held the Certificate ofMedical Practitioner in the People’s Republic of China,whose scope of practice was confined to urological surgery.The urological surgeons involved in the project completed an online questionnaire survey.All the data were collected through the internet.Results:There were a total of 18981 urological surgeons in China in 2015,of whom 15875 from 2602 hospitals participated in this project,with a mean age of 39.64 years old.In 2015,1949631 cases of surgery were performed,including 493723 cases of open surgery,1146444 cases of endoscopic/laparoscopic surgery(robot-assisted laparoscopic surgery were excluded),6259 robot-assisted surgery and other types of urological surgery.Besides,Chinese urological surgeons published 1358 monographs as well as 14558 academic papers,and also obtained 2064 scientific funds in 2015.A total of 92122 person-time participated in academic conferences.Urological surgeons with higher educational degrees as well as higher academic titles and from Eastern China or higher-level hospitals hadmore opportunities to participate in further education and training. | Yinghao Sun Liqun Zhou Junhua Zhang Nianzeng Xing Qian Zhang Xiuzhong Hu Linhui Wang Yinglu Guo | 2020 | Asian Journal of Urology2020，7，2： | 0 |
| 10 | A specific assay for JmjC domain-containing lysine demethylase and its application to inhibitor screening显示文摘Dear Editor, Chromatin is the macromolecular complex of DNA and histone proteins, which provides the scaffold for the packaging of our entire genome. In the basic unit of chromatin, the nucleosomes contain 147 base pairs of DNA, which is wrapped around a histone octamer, with two each of histones H2A, H2B, H3, and H4. Several types of post-translational modifications, including acetylation, phosphorylation, ubiquitination, and methylation, are possible on the tails of histones (Zhang et al., 2015). Combinations of these modifications determine the chromatin structure and transcriptional regulation of genes. Histone methylation is highlighted because of the specific dynamics related to gene regulation. Aberrant histone methylation has been observed in many types of tumors (Albert and Helin, 2010) and may contribute to the neoplastic transformation of cells (Chi et al., 2010). Histone lysine methylation is regulated by two classes of enzymes that have opposing activities: histone lysine methyltransferases (KMTs) and histone lysine demethylases (KDMs). To date, a number of mammalian KDMs have been discovered (Kooistra and Helin, 2012), and they have been separated into two classes: flavin adenine dinucleotide dependent oxidases, which were first discovered in 2004 (Shi et al., 2004), and Jumonji-C (JmjC) domain-containing enzymes, first identified in 2006 (Tsukada et al., 2006). | He Wen Guo Li Yongcan Chen Meiting Li Yafei Lv Zhiming Li Yinglu Li Tianyun Hou Hui Wang Chaohua Liu Xiaopeng Lu Qian Zhu Yantao Bao Ge Liu Xiaofan Li Wei-Guo Zhu | 2019 | Science China(Life Sciences)2019，62，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员