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| 1 | Gene cloning and expression of cadherin in midgut of Helicoverpa armigera and its Cry1A binding region显示文摘Cadherins belong to one of the families of animal glycoproteins responsible for cal-cium-dependent cell-cell adhesion. Recent literatures showed that the cadherin-like in midgut of several insects served as the receptor of Bt toxin Cry1A and the variation of cadherin-like is re-lated to insect’s resistance to Cry1A. The full-length cDNA encoding cadherin-like of Helicoverpa armigera is cloned by degenerate PCR and RACE techniques and the gene was designated as BtR-harm, which is 5581 bp in full-length, encoding 1730 amino acid residues (BtR-harm was deposited in GenBank and the accession number is AF519180). Its predicted molecular weight and isoelectric point were 195.39 kDa and 4.23, respectively. The inferred amino acid sequence includes a signal sequence, 11 cadherin repeats, a membrane-proximal region, a transmem-brane region and a cytoplasmic region. Sequence analysis indicated that the deduced protein sequence was most similar to the cadherin-like from Heliothis virescens with 84.2% identity and highly similar to three other lepidopteran cadherin from Bombyx mori, Manduca sexta and Pectinophora gossypiella, with the sequence identities of 60.3.6%, 57.5% and 51.0%, respec-tively. The cDNA encoding cadherin gene was expressed successfully in E. coli and the recom-binant proteins can bind with Cry1Ac. Truncation analysis and binding experiment of BtR-harm revealed that the Cry1A binding region was a contiguous 244-amino acid sequence, which lo-cated between amino acid 1217 and 1461. Semi-quantitative RT-PCR analysis showed that BtR-harm was highly expressed in midgut of H. armigera, very low expressed in foregut and hindgut and was not expressed in other tissues. After H. armigera producing resistance to Cry1Ac, the expression quantity of BtR-harm significantly decreased in midgut of H. armigera. It is the first confirmation that BtR-harm can function as receptor of Cry1Ac in H. armigera and the binding region was located on a contiguous 244 amino acid sequence, suggesting that the de-crease of expression quantity of BtR-harm is one of the main reasons for H. armigera resistance to Cry1Ac. | WANG Guirong WU Kongming LIANG Gemei GUO Yuyuan | 2005 | Science China(Life Sciences)2005，48，4： | 17 |
| 2 | Cytotoxicity and binding profiles of activated CrylAc and Cry2Ab to three insect cell lines显示文摘 | Jizhen Wei Gemei Liang Kongming Wu Shaohua Gu Yuyuan Guo Xinzhi Ni Xianchun Li | 2018 | Insect Science2018，25，4： | 2 |
| 3 | Resistance alleviation in the larval cotton bollworm to fenvalerate after pretreatment with Bacillus thuringiensis显示文摘 | Tan Weijia Liang Gemei Guo Yuyuan | 1999 | Entomologia Sinica1999，6，1： | 1 |
| 4 | The percentage of CD133+ cells in human colorectal cancer cell lines is influenced by Mycoplasma hyorhinis infection 显示文摘 | Mariotti E Gemei M Mirabelli P | 2010 | BMC Cancer2010，10，： | 1 |
| 5 | Gene clo- ning and sequencing of aminopeptidase N3, a putative receptor for Bacillus thurlngiensis insecticidal CrylAc toxin in Helicov- erpa armigera (Lepidoptera : Noctuidae) 显示文摘 | Wang Guirong Liang Gemei Wu Kongming | 2005 | European Journal of Entomology2005，102，1： | 1 |
| 6 | Toxicity of chlorantraniliprole to Cry1Ac-susceptible and resistant strains of Helicoverpa armigera显示文摘 | Guangchun Cao Qiong Lu Lili Zhang Fang Guo Gemei Liang Kongming Wu Kris A.G. Wyckhuys Yuyuan Guo | 2010 | Pesticide Biochemistry and Physiology2010，，1： | 1 |
| 7 | The Effects of Three Styles of Teaching on the Psychomotor Per- formance of Fifth Grade Children显示文摘 | GOLDBERGER M GEMEY P CHAMBERLAIN J | 1982 | Res Q Exerc Sport1982，53，2： | 1 |
| 8 | The Effects of Direct Teaching Styles on Motor Skill Acquisition of Fifth Grade ChiI- dren显示文摘 | GOOLDBERGER M GEMEY P | 1986 | Res QExercSport1986，57，3： | 1 |
| 9 | Stable oxoborate with edge-sharing BO4 tetrahedra synthesized under ambient pressure 显示文摘 | Jin Shifeng Cai Gemei Wang Wanyan | 2010 | Angew Chem Int Ed2010，49，： | 1 |
| 10 | Computational model of airfow in upper 17 generations of human respiratory tract显示文摘 | Gemei T Ponyavinc V Chena Y | 2008 | Journal of Biomechanics2008，41，9： | 1 |
| 11 | Coke oven gas explosion suppression显示文摘 | ZHANG Zengliang LIN Baiquan LI Gemei | 2013 | Safety Sci- ence2013，55，： | 1 |
| 12 | The percentage of CD133+ cells in human colorectal cancer cell lines is influenced by Mycoplasma hyorhinis infection显示文摘 | MARIOTTI E GEMEI M MIRABELLI P | 2010 | BMC Cancer2010，10，： | 1 |
| 13 | Phoxim resistance in Helicoverpa armigera in China显示文摘 | Wu Kongming Liang Gemei Gu Yuyuan | 1997 | J Econo Ento1997，90，4： | 1 |
| 14 | Postoperative detection of circulating tumor cells predicts tumor recurrence in colorectal cancer patients显示文摘 | Galizia G Gemei M Orditura M | 2013 | J Gastrointest Surg2013，17，10： | 1 |
| 15 | Computational simulations of airflow in an in vitro model of the pedia- tric upper airways显示文摘 | Alien GM Shortall BP Gemei T | 2004 | J Biomech Eng2004，126，5： | 1 |
| 16 | Surface proteomic analysis of differentiated versus stem‐like osteosarcoma human cells显示文摘 | Marica Gemei Claudia Corbo Francesca D'Alessio Rosa Di Noto Renza Vento Luigi Del Vecchio | 2013 | Proteomics2013，，22： | 1 |
| 17 | Tunable emission,energy transfer and thermal stability of Ce^(3+),Tb^(3+) co-doped Na_(2)BaCa(PO_(4))_(2) phosphors显示文摘A series of single Ce^(3+) doped and Ce^(3+) and Tb^(3+) co-doped Na_(2)BaCa(PO_(4))_(2)(NBCP) phosphors was synthesized by conventional solid-stated reaction method.The crystal structure,luminescence properties,thermal stability and energy transfer were carefully investigated.Ce^(3+) is inferred to substitute the Ba^(2+)site in NBCP lattice.The color-tunable emission from blue to green is observed by adjusting Tb^(3+) concentration among NBCP:0.03 Ce^(3+),yTb^(3+) phosphors.The energy transfer behavior from Ce^(3+) to Tb^(3+) ions is both illustrated by co-doped PL spectra and decay curves.The energy transfer efficiency is as high as 91.5%.The mechanism of energy transfer is resonance type of dipole-dipole transition.In this work,the optimal phosphor exhibits the excellent thermal stability which keeps at 94.9% of that initial value at room temperature when temperature reaches to 150℃.The Ce^(3+) and Tb^(3+) co-doped NBCP phosphor is a promising candidate for the application in the general lighting and display fields. | Jiamin Tang Jiayong Si Xiaoyi Fan Yijia Liu Guihua Li Gemei Cai | 2022 | Journal of Rare Earths2022，40，6： | 1 |
| 18 | Combined CD133/CD44 expression as a prognostic indicator of disease-free survival in patients with colorectal cancer显示文摘 | Galizia G Gemei M Del Vecchio L | 2012 | Arch Surg2012，147，1： | 1 |
| 19 | The percentage of CD133+cells in human colorectal cancer cell lines is influenced by Mycoplasma hyorhinis infection显示文摘 | Mariotti E Gemei M Mirabelli P | 2010 | BMC Cancer2010，10，： | 1 |
| 20 | CD66c is a novel marker for colorectal cancer stem cell isolation, and its silencing halts tumor growth in vivo 显示文摘 | Gemei M Mirabelli P Di Noto R | 2013 | Cancer2013，119，4： | 1 |
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