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| 1 | Methodologic research on TIMP-1,TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance显示文摘AIM: To set up a new method to detect tissue inhibitors ofmetalloproteinase1 and -2(TIMP-1 and TIMP-2) in sero ofpatients with hepatic cirrhosis, and to investigate theexpression and location of TIMP-1 and TIMP-2 in liver tissueof patients with hepatic cirrhosis, and the correlationbetween TIMPs in liver and those in sera so as to discusswhether TIMPs can be used ss a diagnosis index of hepaticfibrosisMETHODS: The monoclonal antibodies (McAbs) of TIMP-1and TIMP-2 were used to sensitize erythrocytes, and solid-phase absorption to sensitized erythrocytes (SPASE) wasused to detect TIMP-1 and TIMP-2 in the sera of patients withhepatic cirrhosis. Meanwhile, with the method of in situhybridization and immunohistochemistry, we studied themRNA expression and antigen location of TIMP-1 and TIMP-2in the livers of 40 hepatic cirrhosis patients with pathologicdiagnosis.RESULTS: With SPASE, they were 16.4 % higher in theacute hepatitis group, 33.3 % higher in the chronic hepatitisgroup, and the positive rates were 73.6 % and 61. 2 %respectively in sero of hepatic cirrhosis patients, which wereremarkably higher than those in chronic hepatitis and acutehepatitis group ( P < 0. 001 ). In 40 samples of hepaticcirrhosis tissues, all of them showed positive expression ofTIMP-1 and TIMP-2 mRNA detected withimmunohistochemistry or in situ hybridization (positive ratewas 100 % ). Expression of TIMPs in different degrees couldbe found in liver tissue with cirrhosis. TIMPs were located incytoplasm of liver cells of patients with hepatic cirrhosis.There was a significant correlation between serum TIMPslevel and liver TIMPs level.CONCLUSION: SPASE is a useful method to detect the TIMP-1 and TIMP-2 in sera of patients with hepatic cirrhosis, andTIMP-1 and TIMP-2 can be considered as a useful diagnosticindex of hepatic fibrosis, especially TIMP-1. | Oing-He Nie Yong-Oian Cheng Yu-Mei Xie Yong-Xing Zhou Bai-Xian Guang Yi-Zhan Cao,The Centre of Diagnosis and Treatment for Infectious Disease of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 51 |
| 2 | Effects of Short-Term Forest Bathing on Human Health in a Broad-Leaved Evergreen Forest in Zhejiang Province,China显示文摘Objective To investigate the effects of short-term forest bathing on human health.Methods Twenty healthy male university students participated as subjects and were randomly divided into two groups of 10.One group was sent on a two-night trip to a broad-leaved evergreen forest,and the other was sent to a city area.Serum cytokine levels reflecting inflammatory and stress response,indicators reflecting oxidative stress,the distribution of leukocyte subsets,and plasma endothelin-1 (ET-1) concentrations were measured before and after the experiment to evaluate the positive health effects of forest environments.A profile of mood states (POMS) evaluation was used to assess changes in mood states.Results No significant differences in the baseline values of the indicators were observed between the two groups before the experiment.Subjects exposed to the forest environment showed reduced oxidative stress and pro-inflammatory level,as evidenced by decreased malondialdehyde,interleukin-6,and tumor necrosis factor α levels compared with the urban group.Serum cortisol levels were also lower than in the urban group.Notably,the concentration of plasma ET-1 was much lower in subjects exposed to the forest environment.The POMS evaluation showed that after exposure to the forest environment,subjects had lower scores in the negative subscales,and the score for vigor was increased.Conclusion Forest bathing is beneficial to human health,perhaps through preventive effects related to several pathological factors. | MAO Gen Xiang LAN Xiao Guang CAO Yong Bao CHEN Zhuo Mei HE Zhi Huat LV Yuan Dong WANG Ya Zhen HU Xi Lian WANG Guo Fu YAN Jing | 2012 | Biomedical and Environmental Sciences2012，25，3： | 20 |
| 3 | The ChinaMAP analytics of deep whole genome sequences in 10,588 individuals显示文摘Metabolic diseases are the most common and rapidly growing health issues worldwide.The massive population-based human genetics is crucial for the precise prevention and intervention of metabolic disorders.The China Metabolic Analytics Project(ChinaMAP)is based on cohort studies across diverse regions and ethnic groups with metabolic phenotypic data in China.Here,we describe the centralized analysis of the deep whole genome sequencing data and the genetic bases of metabolic traits in 10,588 individuals from the ChinaMAP.The frequency spectrum of variants,population structure,pathogenic variants and novel genomic characteristics were analyzed.The individual genetic evaluations of Mendelian diseases,nutrition and drug metabolism,and traits of blood glucose and BMI were integrated.Our study establishes a large-scale and deep resource for the genetics of East Asians and provides opportunities for novel genetic discoveries of metabolic characteristics and disorders. | Yanan Cao Lin Li Min Xu Zhimin Feng Xiaohui Sun Jieli Lu Yu Xu Peina Du Tiange Wang Ruying Hu Zhen Ye Lixin Shi Xulei Tang Li Yan Zhengnan Gao Gang Chen Yinfei Zhang Lulu Chen Guang Ning Yufang Bi Weiqing Wang The ChinaMAP Consortium | 2020 | Cell Research2020，30，9： | 20 |
| 4 | Microwave synthesis of Li_2FeSiO_4 cathode materials for lithium-ion batteries显示文摘A novel synthetic method of microwave processing to prepare Li_2FeSiO_4 cathode materials is adopted.The Li_2FeSiO_4 cathode material is prepared by mechanical ball-milling and subsequent microwave processing.Olivin-type Li_2FeSiO_4 sample with uniform and fine particle sizes is successfully and fast synthesized by microwave heating at 700℃in 12 min.And the obtained Li_2FeSiO_4 materials show better electrochemical performance and microstructure than those of Li_2FeSiO_4 sample by the conventional solidstate reaction. | Zhong Dong Peng Yan Bing Cao Guo Rong Hu Ke Du Xu Guang Gao Zheng Wei Xiao | 2009 | Chinese Chemical Letters2009，20，8： | 20 |
| 5 | Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion显示文摘AIM: To investigate RNA interference targeting signal transducer and activator of transcription-3 (STAT3) on invasion of human pancreatic cancer cells. METHODS: We constructed three plasmids of RNA interference targeting the STAT3 gene. After LV (lentivirus)-STAT3siRNA (STAT3 small interfering RNA) the vector was transfected into the human pancreatic cell line, SW1990 and cell proliferation was measured by the MTT assay. Flow cytometry was used to assess cell cycle. Vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) mRNA and protein expression were examined by quantitative PCR and western blotting, respectively. The invasion ability of SW1990 cells was determined by cell invasion assay. RESULTS: We successfully constructed the LVSTAT3siRNA lentivirus vector and proved that it can suppress expression of STAT3 gene in SW1990 cells. RNA interference of STAT3 by the LV-STAT3siRNA construct signifi cantly inhibited the growth of SW1990 cells, in addition to signifi cantly decreasing both VEGF and MMP-2 mRNA and protein expression. Moreover, suppression of STAT3 by LV-STAT3siRNA decreased the invasion ability of SW1990 cells.CONCLUSION: The STAT3 signaling pathway may provide a novel therapeutic target for the treatment of pancreatic cancer since it inhibits the invasion ability of pancreatic cancer cells. | Guang Yan Chen Huang Jun Cao Ke-Jian Huang Tao Jiang Zheng-Jun Qiu | 2009 | World Journal of Gastroenterology2009，15，30： | 19 |
| 6 | Gene therapy for human colorectal carcinoma using human CEA promoter controlled bacterial ADP-ribosylating toxin genes:PEA and DTA gene transfer显示文摘INTRODUCTIONAmajorrequirementforinvivogenetherapyforcanceristotargetthetherapeuticgeneexpresiontomalignanttissuesbyselectingp... | CAO Guang Wen 1, QI Zhong Tian 1, PAN Xin 1, ZHANG Xiao Qin 1, MIAO Xiao Hui 1, FENG Yan 1, LU Xin Hua 1, Shigeki Kuriyama 2 and DU Ping 1 | 1998 | World Journal of Gastroenterology1998，4，5： | 18 |
| 7 | A comparative study between Embosphere~ and conventional transcatheter arterial chemoembolization for treatment of unresectable liver metastasis from GIST显示文摘Objective:Transcatheter arterial chemoembolization(TACE) is a standard treatment for hepatocellular carcinoma(HCC) and/or some unresectable liver metastasis tumors.Hypervascular liver metastatic lesions such as metastasis from gastrointestinal stromal tumor(GIST) are an indication for transcatheter arterial embolization(TAE).The purpose of this study was to evaluate the efficacy and safety of Embosphere~-TAE(Embo-TAE) in comparison with conventional TACE(cTACE) for the treatment of liver metastasis from GIST.Methods:A total of 45 patients who underwent TACE between Aug 2008 and Feb 2013 were enrolled.Patients with GIST who underwent TAE with Embosphere~(n=19) were compared with controls who received cTACE(n=26).The primary end points were treatment response and treatment-related adverse events.The secondary end points were progression-free survival(PFS) and overall survival(OS).Results:The treatment response of Embo-TAE group was significantly higher than that of the cTACE group(P<0.001).The PFS was significantly better in the Embosphere~-group than in the cTACE group(56.6 and 42.1 weeks,respectively;P=0.003).However,there was no statistically significant difference in liver toxicity between the two groups(P>0.05).The median OS in the Embo-TAE group was longer than that in the cTACE group(74.0 weeks,95% CI:68.2-79.8 vs.61.7 weeks,95% CI:56.2-67.2 weeks)(unadjusted P=0.045).The use of Embo-TAE significantly reduced the risk of death in patients with GIST with liver metastases according to the Cox proportional hazards regression model [hazard ratio(HR):0.149;95% CI:0.064-0.475].Conclusions:TAE with Embosphere~ showed better treatment response and delayed tumor progression compared with cTACE.There was no significant difference in treatment-related hepatic toxicities.EmboTAE thus appears to be a feasible and promising approach in the treatment of liver metastasis from GIST. | Guang Cao Xu Zhu Jian Li Lin Shen Renjie Yang Hui Chen Xiaodong Wang Song Gao Haifeng Xu Linzhong Zhu Peng Liu Jianhai Guo | 2014 | Chinese Journal of Cancer Research2014，26，1： | 18 |
| 8 | Low molecular weight heparin suppresses lymphatic endothelial cell proliferation induced by vascular endothelial growth factor C in vitro显示文摘背景胰腺的癌症是最好攻击的人的恶意之一。Lymphangiogenesis 在许多稳固的肿瘤的淋巴节点转移起一个重要作用。低分子的重量肝磷脂(LMWH ) 能禁止房间生长,房间侵略和 angiogenesis,是众所周知的,它是在肿瘤前进的关键过程。我们用反向的抄写聚合酶在胰腺的癌症细胞(PANC-1 ) 测量了脉管的 endothelial 生长因素 C (VEGF-C ) 的表示的方法锁住反应(RT-PCR ) 并且西方的弄污。我们使用了一在里面评估 LMWH 的 anti-lymphangiogenic 效果的 vitroassay, Fragmin,在人的淋巴的 endothelial 上房间(HLEC ) 增长。在一个低集中罐头的结果 Fragmin 有效地禁止 VEGF-C 导致的 HLEC 增长。PANC-1 房间分泌的 VEGF-C 刺激了 HLEC 增长。低集中 LMWH 压制了 VEGF-C 导致的 HLEC 增长但是没影响增长或 PANC-1 房间的 VEGF-C 表示,而 LMWH 的高集中禁止了 PANC-1 房间增长。这些结果建议那 VEGF-C 的结论在支持 HLEC 增长由癌症房间戏释放了一个重要角色。LMWH Fragmin 有 anti-lymphangiogenic 效果并且可以在胰腺的癌症禁止淋巴的转移。 | CAO Guang WU Ji-xiang WU Qing-hua | 2009 | Chinese Medical Journal2009，，13： | 13 |
| 9 | HBx-DNA probe preparation and its application in study of hepatocarcinogenesis显示文摘HBxDNAprobepreparationanditsapplicationinstudyofhepatocarcinogenesisGAOFengGuang,SUNWenSheng,CAOYingLin,ZHANGLiNing,SON... | GAO FengGuang, SUN WenSheng, CAO YingLin, ZHANG LiNing, SONG Jing, LI HuaFen and YAN ShiKunKeywords hepatitis B virus liver neoplasms/etiology carcinoma, hepatocellular/etiology DNA probe | 1998 | World Journal of Gastroenterology1998，4，4： | 12 |
| 10 | Preoperative inflammation-based markers predict early and late recurrence of hepatocellular carcinoma after curative hepatectomy显示文摘BACKGROUND:Recurrence of hepatocellular carcinoma(HCC) after curative resection remains a major cause of treatment failure and tumor-related death.Patterns of HCC recurrence can be categorized into early recurrence and late recurrence which have different underlying mechanisms.In this study,we investigated if simple inflammation-based clinical markers can distinguish patterns of recurrence after curative resection of HCC.METHODS:A retrospective analysis of 223 patients who underwent curative hepatectomy for HCC was performed.Preoperative inflammation-based factors including neutrophil/lymphocyte ratio(NLR),platelet/lymphocyte ratio,γ-glutamyl transferase/alanine aminotransferase ratio,aspartate aminotransferase/platelet ratio index(APRI) and prognostic nutritional index together with other clinicopathologic parameters were evaluated by univariate analysis and multivariate analysis to identify independent prognostic factors.By combining risk factors,predictive models were established to distinguish populations at high risk of early or late recurrence.RESULTS:Age ≤50 years,resection margin ≤1 cm,TNM stage III-IV,NLR>2.75,APRI>0.23 and positive alpha-fetoprotein were independent adverse prognostic factors for early recurrence.Patients with three or more risk factors were at significant higher risk of early recurrence.APRI>0.23 and positive hepatitis B e antigen(HBe Ag) were independent risk factors of late recurrence,the coexistence of high APRI and positive HBe Ag increased the risk of late recurrence.CONCLUSIONS:Preoperative inflammation-based prognostic factors predict early and late recurrence of HCC after curative resection.Different prognostic factor combinations distinguish high-risk populations of early or late HCC recurrence. | Yue Liu Zhong-Xia Wang Yin Cao Guang Zhang Wei-Bo Chen Chun-Ping Jiang | 2016 | Hepatobiliary & Pancreatic Diseases International2016，15，3： | 12 |
| 11 | Transcatheter arterial chemoembolization for gastrointestinal stromal tumors with liver metastases显示文摘AIM:To evaluate the efficacy and safety of transcatheter arterial chemoembolization(TACE) for gastrointestinal stromal tumor(GIST) with liver metastases after the failure of tyrosine kinase inhibitors(TKIs).METHODS:Patients with histologically confirmed CD117-positive GIST with liver metastases who were resistant and/or intolerant to prior imatinib and/or sunitinib and who received TACE for at least one treatment cycle or only best supportive care and TKI reintroduction were eligible for the study.The patients were divided into two groups:those in TACE group received TACE treatment containing 5-20 mL iodized oil and 40-80 mg doxorubicin hydrochloride and TKI reintroduction or best supportive care,those in control group only received TKI reintroduction or best supportive care.The primary end-point was overall survival and the secondary end-points were,progression-free survival(PFS),response rates,and safety.RESULTS:Sixty patients admitted between June 2008 and October 2011 were eligible for this study,including 22 in TACE group and 38 in control group.In the TACE group,12(54.5%) achieved liver partial response,5(22.7%) had stable disease,and 5(22.7%) had liver progressive disease.Disease control rate of liver metastases was 77.3% in the TACE group and 39.5% in the control group.The median liver PFS in TACE group was 47.1 wk(95% CI:23.9-70.3).The median PFS in TACE group was longer than in control group(30.0 wk,95% CI:20.1-39.9 vs 12.9 wk,95% CI:11.9-13.9)(P = 0.0001).The median overall survival in TACE group was also longer than in control group(68.5 wk,95% CI:57.4-79.6 vs 25.7 wk,95% CI:23.2-28.2)(P = 0.0001).TACE treatment significantly reduced the risk of death(hazard ratio:0.109).Patients without extrahepatic metastases treated with TACE had significantly better prognosis.Most of the adverse events were of grade 1 or 2 and tolerable.CONCLUSION:TACE is effective and well tolerated in GIST patients with liver metastases after TKI failure,and it may be an optional treatment for this disease. | Guang Cao Jian Li Lin Shen, Xu Zhu | 2012 | World Journal of Gastroenterology2012，18，42： | 12 |
| 12 | Construction of retroviral vectors to induce a strong expression of human class Ⅰ interferon gene in human hepatocellular carcinoma cells in vitro显示文摘ConstructionofretroviralvectorstoinduceastrongexpresionofhumanclasⅠinterferongeneinhumanhepatocelularcarcinomacelsinvitroCAOG... | CAO Guang Wen 1, GAO Jun 1, DU Ping 1, QI Zhong Tian 1 and KONG Xian Tao 2 | 1997 | World Journal of Gastroenterology1997，3，3： | 9 |
| 13 | Lithofacies paleogeography mapping and reservoir prediction in tight sandstone strata: A case study from central Sichuan Basin, China显示文摘Sand-rich tight sandstone reservoirs are potential areas for oil and gas exploration. However, the high ratio of sandstone thickness to that of the strata in the formation poses many challenges and uncertainties to traditional lithofacies paleogeography mapping. Therefore, the prediction of reservoir sweet spots has remained problematic in the field of petroleum exploration. This study provides new insight into resolving this problem, based on the analyses of depositional characteristics of a typical modern sand-rich formation in a shallow braided river delta of the central Sichuan Basin, China. The varieties of sand-rich strata in the braided river delta environment include primary braided channels,secondary distributary channels and the distribution of sediments is controlled by the successive superposed strata deposited in paleogeomorphic valleys. The primary distributary channels have stronger hydrodynamic forces with higher proportions of coarse sand deposits than the secondary distributary channels. Therefore, lithofacies paleogeography mapping is controlled by the geomorphology, valley locations, and the migration of channels. We reconstructed the paleogeomorphology and valley systems that existed prior to the deposition of the Xujiahe Formation. Following this, rock-electro identification model for coarse skeletal sand bodies was constructed based on coring data. The results suggest that skeletal sand bodies in primary distributary channels occur mainly in the valleys and low-lying areas,whereas secondary distributary channels and fine deposits generally occur in the highland areas. The thickness distribution of skeletal sand bodies and lithofacies paleogeography map indicate a positive correlation in primary distributary channels and reservoir thickness. A significant correlation exists between different sedimentary facies and petrophysical properties. In addition, the degree of reservoir development in different sedimentary facies indicates that the mapping method reliably predicts the distribution of sweet spots. The application and understanding of the mapping method provide a reference for exploring tight sandstone reservoirs on a regional basis. | Yuan Zhong Lu Zhou Xiucheng Tan Chengbo Lian Hong Liu Jijia Liao Guang Hu Mingjie Liu Jian Cao | 2017 | Geoscience Frontiers2017，8，5： | 9 |
| 14 | Polymorphism of Exon 2 of FSHβ Gene and Its Relationship with Reproduction Performance in Two Goat Breeds显示文摘The objectives of the present study were to detect the polymorphism in follicle stimulating hormone beta (FSHβ) gene and investigate the relationship between FSHβ gene and high prolificacy in goats.According to the sequence of ovine FSHβ gene,two pairs of primers were designed to detect single nucleotide polymorphism of exon 2 of FSHβ gene in two goat breeds,Xinong Saanen dairy goat and Boer goat,by PCR-SSCP.The least square mean and genetic variance of different genotypes at polymorphic locus were analyzed,and the selection reaction was forecasted by genetic correlation between marker locus and genetic variance of litter size traits.There was one polymorphic locus occurring in exon 2 (P2) with three genotypes (EE,EF and FF).In Xinong Saanen dairy goat,EE genotype had significantly higher (P < 0.05) litter size than EF and FF genotype in the first to fourth parity and average parity;EF genotype had significantly higher (P < 0.05) litter size than FF genotype in the second and average parity.In Boer goat,EE genotype had significantly higher (P < 0.01) litter size than EF and FF genotype in the second to fourth parity and average parity;EF genotype had significantly higher (P < 0.05) litter size than FF genotype in average parity.In Xinong Saanen dairy and Boer goat,the heredity of litter size was mainly influenced by genetic additive effect and the EE genotype is a favorable marker genotype for litter size in these two breeds. | AN Xiao-peng HAN Dan HOU Jin-xing LI Guang WANG Ya-na LI Ling ZHU Guang-qin WANG Jian-gang SONG Yu-xuan CAO Bin-yun | 2010 | Agricultural Sciences in China2010，9，6： | 9 |
| 15 | Visible calculation of mining index based on stope 3D surveying and block modeling显示文摘Aimed at the CMS laser scanning theory and characteristic, a combined actual situation of stope N4-5 of Fankou Lead-Zinc Mine and complementary monitoring of the stope were carried out by carefully choos- ing two measuring points. The cavity 3D visible model was created by large-scale mining industry soft- ware Surpac after changing the measured data. The stope mine design model, bottom structural model and backfill model of the south and north sides of the stope N4-5 were established according to the stope design data. On this basis, the stope block model was established, and then block attribute was estimated. The amount the ore remains, mullock, backfill and total mined ore were calculated through the solid model restrains. Finally, the stope mining dilution rate and loss rate reached 8.2% and 1.47%, respectively. The practice indicates that the mining index visible calculation method based on cavity 3D monitoring and stope block modeling can make up the deficiency of adopting the solid model to directly carry out the Boolean operation. The stope mining indexes obtained by this method are accurate and reliable, and can be used to guide the actual production management and estimate the mining quality. | Liu Xiaoming Luo Zhouquan Yang Biao Lu Guang Cao Shengxiang Jiang Xinjian | 2012 | International Journal of Mining Science and Technology2012，22，2： | 7 |
| 16 | Preoperative serum liver enzyme markers for predicting early recurrence after curative resection of hepatocellular carcinoma显示文摘BACKGROUND:Early recurrence of hepatocellular carcinoma(HCC)is associated with worse prognosis after liver resection This study aimed to investigate the prognostic value of com mon liver enzyme markers in HCC early recurrence after cu rative hepatectomy and to establish a simple predictive model for HCC early recurrence. | Zhong-Xia Wang Chun-Ping Jiang Yin Cao Guang Zhang Wei-Bo Chen Yi-Tao Ding | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，2： | 7 |
| 17 | Active dry yeast supplementation improves the growth performance,rumen fermentation, and immune response of weaned beef calves显示文摘The objective of this experiment was to investigate the potential benefits of active dry yeast(ADY)on the growth performance,rumen fermentation,nutrient digestibility,and serum parameters of weaned beef calves.Thirty Simmental crossbred male calves(body weight=86.47±4.41 kg and 70±4 d of age)were randomly divided into 2 groups:control(CON)(fed basal ration)and ADY(fed basal ration and 5 g/d ADY per calf).The dietary concentrate-to-roughage ratio was 35:65.All the calves were regularly provided rations 3 times a day at 07:00,13:00,and 19:00 and had free access to wate r.The experiment lasted for 60 d.The average daily gain of ADY group was higher(P=0.007)than that of the CON group,and the ratio of feed intake to average daily gain in the ADY group was reduced(P=0.022)as compared to the CON group.The concentration of ruminal ammonia-N was higher(P=0.023)in the CON group than that in the ADY group,but an opposite trend of microbial protein was found between the 2 groups.Also,the ruminal concentrations of propionate and butyrate were higher(P<0.05)in the ADY group than those in the CON group.Calves fed ADY exhibited higher(P<0.05)crude protein and neutral detergent fiber digestibility.Supplementation of ADY increased(P<0.05)the contents of glucose,glutathione peroxidase,superoxide dismutase,immunoglobulin A,immunoglobulin M,and interleukin 10 in the serum of calves,but an opposite trend was observed in malondialdehyde,interleukin 1 beta,and tumor necrosis factor alpha contents between the 2 groups.In conclusion,dietary supplementation with ADY could improve the growth performance,rumen fermentation,nutrient digestibility,antioxidant ability,and immune response of weaned beef calves. | Jian Ma Cheng Wang Zhisheng Wang Guang Cao Rui Hu Xueying Wang Huawei Zou Kun Kang Quanhui Peng Bai Xue Lizhi Wang Yueming Zhu Xiaopeng Zhu | 2021 | Animal Nutrition2021，，4： | 7 |
| 18 | Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth显示文摘AIM:To evaluate the effect of RNA interference (RNAi) mediated silence of signal transduction and activation of transcription (STAT)3 on the growth of human pancreatic cancer cells both in vitro and in vivo.METHODS:STAT3 specific shRNA was used to silence the expression of STAT3 in pancreatic cancer cell line SW1990.The anti-growth effects of RNAi against STAT3 were studied in vitro and in experimental cancer xenografts in nude mice.The potential pathways involved in STAT3 signaling were detected using reverse transcription polymerase chain reaction and western blotting.RESULTS:The expression of the STAT3 was inhibited using RNAi in SW1990 cells.RNAi against STAT3 inhibited cell proliferation,induced cell apoptosis and significantly reduced the levels of CyclinD1 and Bcl-xL when compared with parental and control vector-transfected cells.In vivo experiments showed that RNAi against STAT3 inhibited the tumorigenicity of SW1990 cells and significantly suppressed tumor growth when it was directly injected into tumors.CONCLUSION:STAT3 signaling pathway plays an important role in the progression of pancreatic cancer,and silence of STAT3 gene using RNAi technique may be a novel therapeutic option for treatment of pancreatic cancer. | Chen Huang Guang Yang Tao Jiang Jun Cao Ke-Jian Huang Zheng-Jun Qiu | 2011 | World Journal of Gastroenterology2011，17，25： | 6 |
| 19 | Immobilized thrombin on X-ray radiopaque polyvinyl alcohol/chitosan embolic microspheres for precise localization and topical blood coagulation显示文摘Trans-catheter arterial embolization(TAE)plays an important role in treating various diseases.The available embolic agents lack X-ray visibility and do not prevent the reflux phenomenon,thus hindering their application for TAE therapy.Herein,we aim to develop a multifunctional embolic agent that combines the X-ray radiopacity with local procoagulant activity.The barium sulfate nanoparticles(BaSO4 NPs)were synthesized and loaded into the polyvinyl alcohol/chitosan(PVA/CS)to prepare the radiopaque BaSO4/PVA/CS microspheres(MS).Thereafter,thrombin was immobilized onto the BaSO4/PVA/CS MS to obtain the thrombin@BaSO4/PVA/CS MS.The prepared BaSO4/PVA/CS MS were highly spherical with diameters ranging from 100 to 300μm.In vitro CT imaging showed increased X-ray visibility of BaSO4/PVA/CS MS with the increased content of BaSO4 NPs in the PVA/CS MS.The biocompatibility assessments demonstrated that the MS were non-cytotoxic and possessed permissible hemolysis rate.The biofunctionalized thrombin@BaSO4/PVA/CS MS showed improved hemostatic capacity and facilitated hemostasis in vitro.Additionally,in vivo study performed on a rabbit ear embolization model confirmed the excellent X-ray radiopaque stability of the BaSO4/PVA/CS MS.Moreover,both the BaSO4/PVA/CS and thrombin@BaSO4/PVA/CS MS achieved superior embolization effects with progressive ischemic necrosis on the ear tissue and induced prominent ultrastructural changes in the endothelial cells.The findings of this study suggest that the developed MS could act as a radiopaque and hemostatic embolic agent to improve the embolization efficiency. | Xiaohong Li Xiongfa Ji Kun Chen Muhammad Wajid Ullah Basen Li Jiameng Cao Lin Xiao Jun Xiao Guang Yang | 2021 | Bioactive Materials2021，6，7： | 6 |
| 20 | Eogenetic Karst in Interbedded Carbonates and Evaporites and Its Impact on Hydrocarbon Reservoir: A New Case from Middle Triassic Leikoupo Formation in Sichuan Basin, Southwest China显示文摘Karst in interbedded carbonates and evaporites has been reported to have important and complex impacts on reservoir. It is significant for exploration and karst geology. Here, we report such a new case from Middle Triassic Leikoupo Formation of Sichuan Basin, Southwest China. Stratigraphic incompleteness and the occurrence of unconformity provide evidence for the presence of eogenetic karst. Under the impact of this eogenetic karst, residual weathered and solution-collapse breccia, solution pores and silicification and dedolomitization have been observed. Classic stratigraphic zonation of karst is not readily distinguishable, which is ascribed to the stratigraphic collapse of carbonate rocks resulting from the dissolution of evaporites by lateral subsurface fluid flow. In terms of impact on reservoir quality, karst can generally improve the initial physical property of the porous layers in theory. However, subsurface fluid flow dissolved the evarporitic beds and facilitated the collapse of overlying strata. As a consequence, the lateral continuity of the reservoirs would be destroyed, and relatively high-quality reservoirs can only be developed with little collapse of overlying strata, reflecting reservoir heterogeneities. This may be a general feature of reservoir formation under the impact of karst in interbedded carbonates and evaporites. | Hong Liu Xiucheng Tan Ling Li Xiong Ding Jian Cao Guang Yang Teng Ma | 2019 | Journal of Earth Science2019，30，5： | 5 |
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