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| 1 | A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure显示文摘因为他们的人的白血球抗原(HLA ) haplotype 将强烈增加与房间线的相对更小的队为某些人口匹配的度,同型结合的人的胚胎的干细胞(hESCs ) 被认为是为 hESC 银行的更好的房间来源。同型结合的 hESCs 能从单性生殖的胚胎被产生,但是仅仅异质接合的 hESCs 用当前的策略被建立了没有第二极的身体挤出,人工地激活卵母细胞。这里,我们在 vitro 授精处理从 one-pronuclear 卵母细胞追随者例程报导一根人的同型结合的转换字符线(chHES-32 ) 的第一成功的推导。chHES-32 房间与正常 hESCs 表示普通标记和基因。他们处于一个无差别的状态被宣传超过一年(>P50 ) 了并且维持了 46 的稳定的 karyotype, XX。当在 vivo 并且在 vitro 区分了时, chHES-32 房间能从所有三胚胎的细菌层形成衍生物。五父亲一般地表示的印的基因和他们与卵母细胞施主相同的 HLA 遗传型的几乎无法发现的表示显示了他们的单性生殖的起源。用染色体宽的单个核苷酸的多型性分析并且 DNA 采指纹, chHES-32 房间的 homozygosity 进一步被证实。结果显示“讨厌”的 one-pronuclear 卵母细胞可能是为人的同型结合、单性生殖的转换字符的潜在的来源,并且为从单性生殖的 haploid 卵母细胞获得同型结合的 hESC 线建议了其他的策略。 | Ge Lin | 2007 | Cell Research2007，17，12： | 15 |
| 2 | Bacterial diversity and distribution in the southeast edge of the Tengger Desert and their correlation with soil enzyme activities显示文摘The nature of microbial communities and their relation to enzyme activities in desert soils is a neglected area of investigation. To address this, the bacterial diversity and distribution and soil physico-chemical factors were investigated in the soil crust, the soil beneath the crust and rhizosphere soil at the southeast edge of the Tengger Desert, using the denaturing gradient gel electrophoresis of 16S rRNA genes amplified by the polymerase chain reaction. Phylogenetic analysis of the sequenced DGGE bands revealed a great diversity of bacteria. The Proteobacteria, consisting of the α, β, and γ subdivisions, were clearly the dominant group at all depths and in rhizosphere soil. Analysis of the enzyme activities indicated that the rhizosphere soil of Caragana korshinskii exhibited the highest protease and polyphenol oxidase activities, and in the soil crust there were increased activities of catalase, urease, dehydrogenase and sucrase. The bacterial community abundance closely correlated with soil enzyme activities in different soils. The presence of Cyanobacteria correlated with significant increases in protease, catalase and sucrase in the soil crust, and increased urease in the rhizosphere soil of Artemisia ordosica. The occurrence of Acidobacteria was associated with significant increases in urease, dehydrogenase, and sucrase in the rhizosphere soil of C. korshinski. The presence of γ-Proteobacteria correlated with a significant increase in polyphenol oxidase in the rhizosphere soil of A. ordosica. The study indicated a close relationship between the soil bacterial community and soil enzymes, suggesting the necessity of further investigations into bacterial function in this desert ecosystem. | Wei Zhang Gaosen Zhang Guangxiu Liu Zhibao Dong Tuo Chen Manxiao Zhang Paul J.Dyson Lizhe An | 2012 | Journal of Environmental Sciences2012，24，11： | 14 |
| 3 | Fabrication of submicron barium sulfate aggregates in the presence of ethylenediaminetetraacetic acid anions显示文摘Barium sulfate aggregates with an average size of 0.5 μm were synthesized at pH 7,directed by ethylenediaminetetraacetic acid(EDTA) anions.The particle morphology,chemical composition,and size distribution of the BaSO_4 aggregates were characterized.The as-synthesized BaSO_4 particles were spherical and comprised many interconnected nanoballs,of which the surface properties were affected by the EDTA anions.The adsorption of EDTA anions reversed the charge and weakened the surface polarity of BaSO_4,instigating the formation of aggregates by a self-assembly and transformation process.The resulting BaSO_4 particles at pH 9-10 were ellipsoidal and featured smooth surfaces.Based on the zeta potential of BaSO_4,variations in the morphology induced by changes in pH were closely related to the adsorption of mono- and multi-valent anions onto the electrical double layer of BaSO_4. | Linna Hu Guangxiu Wang Chun Yang Rong Cao | 2015 | Particuology2015，13，5： | 11 |
| 4 | Analysis of Spectral Characteristics Based on Optical Remote Sensing and SAR Image Fusion显示文摘Because of cloudy and rainy weather in south China, optical remote sensing images often can't be obtained easily. With the regional trial results in Baoying,Jiangsu province, this paper explored the fusion model and effect of ENVISAT/SAR and HJ-1A satellite multispectral remote sensing images. Based on the ARSIS strategy, using the wavelet transform and the Interaction between the Band Structure Model(IBSM), the research progressed the ENVISAT satellite SAR and the HJ-1A satellite CCD images wavelet decomposition, and low/high frequency coefficient reconstruction, and obtained the fusion images through the inverse wavelet transform.In the light of low and high-frequency images have different characteristics in different areas, different fusion rules which can enhance the integration process of selfadaptive were taken, with comparisons with the PCA transformation, IHS transformation and other traditional methods by subjective and the corresponding quantitative evaluation. Furthermore, the research extracted the bands and NDVI values around the fusion with GPS samples, analyzed and explained the fusion effect. The results showed that the spectral distortion of wavelet fusion, IHS transform, PCA transform images was 0.101 6, 0.326 1 and 1.277 2, respectively and entropy was14.701 5, 11.899 3 and 13.229 3, respectively, the wavelet fusion is the highest.The method of wavelet maintained good spectral capability, and visual effects while improved the spatial resolution, the information interpretation effect was much better than other two methods. | Weiguo LI Nan JIANG Guangxiu GE | 2014 | Agricultural Science & Technology2014，15，11： | 4 |
| 5 | Assessment of organic compost and biochar in promoting phytoremediation of crude-oil contaminated soil using Calendula officinalis in the Loess Plateau, China显示文摘The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has been extremely destroyed due to the over-exploitation of crude-oil resources.Remediation of crude-oil contaminated soil in this area remains to be a challenging task.In this study,in order to elucidate the effects of organic compost and biochar on phytoremediation of crude-oil contaminated soil(20 g/kg)by Calendula officinalis,we designed five treatments,i.e.,natural attenuation(CK),planted C.officinalis only(P),planted C.officinalis with biochar amendment(PB),planted C.officinalis with organic compost amendment(PC),and planted C.officinalis with co-amendment of biochar and organic compost(PBC).After 152 d of cultivation,total petroleum hydrocarbons(TPH)removal rates of CK,P,PB,PC and PBC were 6.36%,50.08%,39.58%,73.10%and 59.87%,respectively.Shoot and root dry weights of C.officinalis significantly increased by 172.31%and 80.96%under PC and 311.61%and 145.43%under PBC,respectively as compared with P(P<0.05).Total chlorophyll contents in leaves of C.officinalis under P,PC and PBC significantly increased by 77.36%,125.50%and 79.80%,respectively(P<0.05)as compared with PB.Physical-chemical characteristics and enzymatic activity of soil in different treatments were also assessed.The highest total N,total P,available N,available P and SOM(soil organic matter)occurred in PC,followed by PBC(P<0.05).C.officinalis rhizospheric soil dehydrogenase(DHA)and polyphenol oxidase(PPO)activities in PB were lower than those of other treatments(P<0.05).The values of ACE(abundance-based coverage estimators)and Chao 1 indices for rhizospheric bacteria were the highest under PC followed by PBC,P,PB and CK(P<0.05).However,the Shannon index for bacteria was the highest under PC and PBC,followed by P,PB and CK(P<0.05).In terms of soil microbial community composition,Proteiniphilum,Immundisolibacteraceae and Solimonadaceae were relatively more abundant under PC and PBC.Relative abundances of Pseudallescheria,Ochroconis,Fusarium,Sarocladium,Podospora,Apodus,Pyrenochaetopsis and Schizothecium under PC and PBC were higher,while relative abundances of Gliomastix,Aspergillus and Alternaria were lower under PC and PBC.As per the nonmetric multidimensional scaling(NMDS)analysis,application of organic compost significantly promoted soil N and P contents,shoot length,root vitality,chlorophyll ratio,total chlorophyll,abundance and diversity of rhizospheric soil microbial community in C.officinalis.A high p H value and lower soil N and P contents induced by biochar,altered C.officinalis rhizospheric soil microbial community composition,which might have restrained its phytoremediation efficiency.The results suggest that organic compost-assisted C.officinalis phytoremediation for crude-oil contaminated soil was highly effective in the Loess Plateau,China. | WANG Jincheng JING Mingbo ZHANG Wei ZHANG Gaosen ZHANG Binglin LIU Guangxiu CHEN Tuo ZHAO Zhiguang | 2021 | Journal of Arid Land2021，13，6： | 4 |
| 6 | Microbial diversity in the saline-alkali soil of a coastal Tamarix chinensis woodland at Bohai Bay, China显示文摘Soil salinization or alkalization is a form of soil desertification. Coastal saline-alkali soil represents a type of desert and a key system in the network of ecosystems at the continent-ocean interface. Tamarix chinensis is a drought-tolerant plant that is widely distributed in the coastal saline-alkali soil of Bohai Bay, China. In this study, we used 454 pyrosequencing techniques to investigate the characteristics and distribution of the microbial diversity in coastal saline-alkali soil of the T. chinensis woodland at Bohai Bay. A total of 20,315 sequences were obtained, representing 19 known bacterial phyla and a large proportion of unclassified bacteria at the phylum level. Proteobacteria, Acidobacteria and Actinobacteria were the predominant phyla. The coverage of T. chinensis affected the microbial composition. At the phylum level, the relative abundance of γ-Proteobacteria and Bacteroidetes decreased whereas Actinobacteria increased with the increasing coverage of T. chinensis. At the genus level, the proportions of Steroidobacter, Lechevalieria, Gp3 and Gp4 decreased with the increase of the vegetation coverage whereas the proportion of Nocardioides increased. A cluster analysis showed that the existing T. chinensis changed the niches for the microorganisms in the coastal saline-alkali soil, which caused changes in the microbial community. The analysis also distinguished the microbial community structure of the marginal area from those of the dense area and sparse area. Furthermore, the results also indicated that the distance to the seashore line could also affect certain groups of soil bacteria in this coastal saline-alkali soil, such as the family Cryomorphaceae and class Flavobacteria, whose population decreased as the distance increased. In addition, the seawater and temperature could be the driving factors that affected the changes. | LIU Wanqiu ZHANG Wei LIU Guangxiu ZHANG Yanhua ZHANG Gaosen | 2016 | Journal of Arid Land2016，8，2： | 4 |
| 7 | The effect of carbon nanotubes on osteogenic functions of adipose-derived mesenchymal stem cells in vitro and bone formation in vivo compared with that of nano-hydroxyapatite and the possible mechanism显示文摘It has been well recognized that the development and use of artificial materials with high osteogenic ability is one of the most promising means to replace bone grafting that has exhibited various negative effects.The biomimetic features and unique physiochemical properties of nanomaterials play important roles in stimulating cellular functions and guiding tissue regeneration.But efficacy degree of some nanomaterials to promote specific tissue formation is still not clear.We hereby comparatively studied the osteogenic ability of our treated multiwalled carbon nanotubes(MCNTs)and the main inorganic mineral component of natural bone,nano-hydroxyapatite(nHA)in the same system,and tried to tell the related mechanism.In vitro culture of human adiposederived mesenchymal stem cells(HASCs)on the MCNTs and nHA demonstrated that although there was no significant difference in the cell adhesion amount between on the MCNTs and nHA,the cell attachment strength and proliferation on the MCNTs were better.Most importantly,the MCNTs could induce osteogenic differentiation of the HASCs better than the nHA,the possible mechanism of which was found to be that the MCNTs could activate Notch involved signaling pathways by concentrating more proteins,including specific bone-inducing ones.Moreover,the MCNTs could induce ectopic bone formation in vivo while the nHA could not,which might be because MCNTs could stimulate inducible cells in tissues to form inductive bone better than nHA by concentrating more proteins including specific bone-inducing ones secreted from M2 macrophages.Therefore,MCNTs might be more effective materials for accelerating bone formation even than nHA. | Zhipo Du Xinxing Feng Guangxiu Cao Zhending She Rongwei Tan Katerina E.Aifantis Ruihong Zhang Xiaoming Li | 2021 | Bioactive Materials2021，6，2： | 3 |
| 8 | Novel biallelic PCNT deletion causing microcephalic osteodysplastic primordial dwarfism type II with congenital heart defect显示文摘Dear Editor, Microcephalic osteodysplastic primordial dwarfism type Ⅱ (MOPD Ⅱ )is characterized by developmental retardation, wherein the affected individuals usually present with intrauterine growth retardation and preterm birth (Majewski et al.,1982;Willems et al.,2010).This leads to an average weight of <1,500g at birth and extremely restricted postnatal growth (Hall et al.,2004;Rauch,2011).Clinical manifestations ofMOPD Ⅱ include microcephaly,disproportionately short stature,mild skeletal dysplasia,unusual facial features including a prominent nose,prominent eyes in infancy and early childhood,some affected individuals exhibit slightly reduced intellectual development and cerebral vascular malformations (Willems et al.,2010;Li et al.,2015;Sam et al.,2015). | Lanlan Meng Chaofeng Tu Guangxiu Lu Ge Lin Yueqiu Tan | 2019 | Science China(Life Sciences)2019，62，1： | 2 |
| 9 | Human umbilical cord mesenchymal stem cells for psoriasis:a phase 1/2a,single-arm study显示文摘Psoriasis is a common,chronic immune-mediated systemic disease that had no effective and durable treatment.Mesenchymal stem cells(MSCs)have immunomodulatory properties.Therefore,we performed a phase 1/2a,single-arm clinical trial to evaluate the safety and efficacy of human umbilical cord-derived MSCs(UMSCs)in the treatment of psoriasis and to preliminarily explore the possible mechanisms.Seventeen patients with psoriasis were enrolled and received UMsC infusions.Adverse events,laboratory parameters,PASl,and PGA were analyzed.We did not observe obvious side effects during the treatment and 6-month follow-up.A total of 47.1%(8/17)of the psoriasis patients had at least 40%improvement in the PASl score,and 17.6%(3/17)had no sign of disease or minimal disease based on the PGA score.And the efficiency was 25%(2/8)for males and 66.7%(6/9)for females.After UMSC transplantation(UMSCT),the frequencies of Tregs and CD4^(+)memory T cells were significantly increased,and the frequencies of T helper(Th)17 and CD4^(+)naive T cells were significantly decreased in peripheral blood(PB)of psoriasis patients.And all responders showed significant increases in Tregs and CD4^(+)memory T cells,and significant decreases in Th17 cells and serum IL-17 level after UMsCT.And baseline level of Tregs in responders were significantly lower than those in nonresponders.In conclusion,allogeneic UMSCT is safe and partially effective in psoriasis patients,and level of Tregs may be used as a potent biomarker to predict the clinical efficacy of UMSCT.Trial registration Clinical Trials NCT03765957. | Lamei Cheng Siqi Wang Cong Peng Xiao Zou Chao Yang Hua Mei Chuang Li Xian Su Na Xiao Qi Ouyang Mi Zhang Qiaolin Wang Yan Luo Minxue Shen Qun Qin Honglin Wang Wu Zhu Guangxiu Lu Ge Lin Yehong Kuang Xiang Chen | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 2 |
| 10 | Silencing of IKKε using siRNA inhibits proliferation and invasion ofglioma cells in vitro and in vivo显示文摘 | Huibing Li Lingchao Chen Anling Zhang Guangxiu Wang Lei Han Kai Yu Peiyu Pu Chunsheng Kang Qiang Huang | 2012 | International Journal of Oncology2012，，1： | 1 |
| 11 | MicroRNA-221 and -222 Regulate Radiation Sensitivity by Targeting the PTEN Pathway显示文摘 | Chunzhi Zhang Chunsheng Kang Ping Wang Yongzhen Cao Zhonghong Lv Shizhu Yu Guangxiu Wang Anling Zhang Zhifan Jia Lei Han Chunying Yang Hiromichi Ishiyama Bin S. Teh Bo Xu Peiyu Pu | 2011 | International Journal of Radiation Oncology Biology Physics2011，，1： | 1 |
| 12 | Bacterial diversity in the sediment of Crescent Moon Spring,Kumtag Desert,Northwest China显示文摘Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs(Operational Taxonomic Units) in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI Gen Bank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems(including desert lakes) worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacter were the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs(including OTU3615, OTU6535, and OTU6646) between sediment of Crescent Moon Spring and various origins from NCBI Gen Bank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems(including desert lakes) worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and p H values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems. | ZHANG Wei ZHANG Gaosen WU Xiukun LIU Guangxiu DONG Zhibao QU Jianjun WANG Yun CHEN Tuo | 2017 | Journal of Arid Land2017，9，2： | 1 |
| 13 | MiRNA-451 plays a role as tumor suppressor in human glioma cells显示文摘 | Yang Nan Lei Han Anling Zhang Guangxiu Wang Zhifan Jia Yang Yang Xiao Yue Peiyu Pu Yue Zhong Chunsheng Kang | 2010 | Brain Research2010，，： | 1 |
| 14 | MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 inhuman glioma显示文摘 | Yuan Tian Yang Nan Lei Han Anling Zhang Guangxiu Wang Zhifan Jia Jianwei Hao Peiyu Pu Yue Zhong Chunsheng Kang | 2012 | International Journal of Oncology2012，，4： | 1 |
| 15 | Syn thesis and characterization of La2O3/TiO2-xFx and the visible light photocatalytic oxidation of 4-chlorophenol 显示文摘 | Cao Guangxiu Li Yaogang Zhang Qinghong | 2010 | Journal of Hazardous Materials2010，178，： | 1 |
| 16 | Quantitative characteristics of microorganisms in permafrost at different depths and their relation to soil physicochemical properties显示文摘Microorganisms in permafrost can live in cold environments due to coadapted physicochemical processes in this environment.In this paper,the relation between microbial number and soil physicochemical properties at the headwaters area of the Urumqi River is analyzed by using fluorescence microscopy counting and oligo-culture techniques.In total,20 samples from a 200-cm permafrost core were used as study materials.The study reveals that the number of culturable bacteria has a significantly positive correlation with soil water content,total carbon and total nitrogen concentrations,and a significantly negative correlation with soil pH value.In addition,the ratio of culturable bacteria to total cell number decreases with depths.The results demonstrate that the number of culturable bacteria in permafrost is closely correlated with soil physicochemical properties and depositional age. | Wei Zhang XiaoPei Dong GuangXiu Liu GaoSen Zhang XiuKun Wu XiSheng Tai HaoZhi Long BaoGui Zhang | 2012 | Research in Cold and Arid Regions2012，4，2： | 1 |
| 17 | Variations in soil culturable bacteria communities and biochemical characteristics in the Dongkemadi Glacier forefield along a chronosequence 显示文摘 | Liu Guangxiu Hu Ping Zhang Wei | 2012 | Folia Microbiologica2012，57，： | 1 |
| 18 | Glycerol-3-phosphate acyltransferase 4 gene is involved in mouse spermatogenesis显示文摘Glycerol-3-phosphate acyltransferase (GPAT ) 由把 glycerol-3-phosphate 变换成 lysophosphatidic 酸(LPA ) 催化 de novo triacylglycerol 合成的首先承诺的步。LPA 是调停的 mitogen 多重细胞的过程包括房间增长。四 GPAT isoforms 迄今为止被克隆。GPAT4 强烈在老鼠睾丸被表示。反向的 transcriptionpolymerase 链反应(PCR ) ,即时 PCR,并且在 situ,杂交(ISH ) 被用来分析 GPAT4 表达式并且在出生后的开发期间在鼠标睾丸本地化表示房间类型。GPAT4 cDNA 被插入到 pcDNA4/His 构造 recombinant 向量,它是进一根老鼠 spermatogonial 房间线(GC-1spg ) 的 transfected。GPAT4 首先出生后地在 2 个星期在老鼠被表示。表示从第三个星期是丰富的,在星期 56 点的 plateaued 然后在成年人在高水平维持了。ISH 表明那 GPAT4 基因在以后的精子形式期间在成熟分裂期间然而并非在伸长的 spermatids 在 spermatocytes 并且在 spermatids 附近富有地被表示。GC-1spg 房间与 GPAT4 在 transfection 以后在增长显示出显著增加;房间周期分析在在 S 阶段的 G0/G1 阶段和增加在房间的百分比显示出减少。因此, GPAT4 可能在精子发生起一个重要作用,在特别中间成熟分裂。 | Qingming Qiu Gang Liu Weina Li Qiuwen Shi Fuxi Zhu Guangxiu Lu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 1 |
| 19 | Relationship between microdeletion on Y chromosome and patients with idiopathic azoospermia and severe oligozoospermia in the Chinese显示文摘 | Fu Junjiang Li Luyun Lu Guangxiu | 2002 | J Chn Med2002，115，： | 1 |
| 20 | Long Y chromosome is not a fe- tal loss risk 显示文摘 | Hongchuan Nie Guangxiu Lu | 2011 | J Assist Reprod Genet2011，28，2： | 1 |
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