|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection显示文摘The highly efficient novel methods to produce transgenic chickens were established by directly in-jecting the recombinant plasmid containing green fluorescent protein (GFP) gene into the cock's testis termed as testis-medianted gene transfer (TMGT), and transplanting transfected spermatogonial stem cells (TTSSCs). For the TMGT approach,four dosages of pEGFP-N1 DNA/cationic polymer complex were injected intratesticularly. The results showed: (1) 48 h after the injection,the percentages of testis cells expressing GFP were 4.0%, 8.7%, 10.2% and 13.6% in the 50, 100, 150 and 200 μg/mL group, re-spectively. The difference from the four dosage groups was significant (P<0.05). On day 25 after the injection, a dosage-dependent and time-dependent increase in the number of transgenic sperm was observed. The percentages of gene expression reached the summit and became stable from day 70 to 160, being 12.7%, 12.8%, 15.9% and 19.1%, respectively. The difference from the four dosage groups was also significant (P<0.05). (2) 70 d after the injection, strong green fluorescent could be observed in the seminiferous tubules by whole-mount in-situ hybridization. (3) 70 d after the injection, the semen was collected and used to artificially inseminate wild-type females. The blastoderms of F1 and F2 transgenic chicken expressed GFP were 56.2% (254/452) and 53.2% (275/517), respectively. The detec-tion of polymerase chain reaction (PCR) of F1 and F2 transgenic chicken blood genomic DNA showed that 56.5% (3/23) of F1 and 52.9% (9/17) of F2 were positive. Southern blot showed GFP DNA was in-serted in their genomic DNAs. (4) Frozen whole mount tissue sections of F1 and F2 transgenic chicken liver, heart, kidney and muscle showed that the rates of green fluorescent positive were between 50.0% and 66.7%. (5) With the TTSSCs method, SSCs ex vivo transfected with GFP were transplanted into recipient roosters whose endogenic SSCs had been resoluted. The donor SSCs settled and GFP ex-pression became readily detectable in the frozen whole mount tissue sections of recepient testes. Moreover, sperms carrying GFP could be produced normally. The results of artificially inseminating wild-type females with these sperms showed 12.5% (8/64) of offspring embryo expressed GFP and 11.1% (2/18) hatched chicks were tested transgenic. Our data therefore suggest TMGT and TTSSCs are the feasible methods for the generation of transgenic chickens. | LI BiChun SUN GuoBo SUN HuaiChang XU Qi GAO Bo ZHOU GuanYue ZHAO WenMing WU XinSheng BAO WenBin YU Fei WANG KeHua CHEN GuoHong | 2008 | Science China(Life Sciences)2008，51，8： | 13 |
| 2 | EMTP-type Program Realization of Krylov Subspace Based Model Reduction Methods for Large-Scale Active Distribution Network显示文摘Effective model reduction methods are required to deal with new challenges in active distribution network simulations that are on a large scale and have complicated structures.In the development of advanced electromagnetic transient simulation programs,automated model reduction plays an important role.This paper proposes an automated realization algorithm for the Krylov subspace based model reduction methods of an active distribution network with which the reduced model can be automatically established according to a given threshold of reduction error.The combined state-space nodal analysis framework is employed to apply the automated model reduction algorithm in popular EMTP-type simulation programs.Simulations are performed using PSCAD and a self-developed program to show the feasibility and validity of the proposed methods. | Chengshan Wang Hao Yu Peng Li Jianzhong Wu Chengdi Ding Guanyu Song Dun Lin Feng Xing | 2015 | CSEE Journal of Power and Energy Systems2015，1，1： | 8 |
| 3 | A direct H2O2 production based on hollow porous carbon sphere-sulfur nanocrystal composites by confinement effect as oxygen reduction electrocatalysts显示文摘Carbon-sulfur composites have draw n in creasing interest in various fields including electrocatalysis because of their unique structures.However,carb on-sulfur composite with tiny sulfur nano crystals has still received little attention.Herein,hollow porous carb on sphere-sulfur composite(HPCS-S)which possesses excellent electrochemical performance towards H2O2 has been prepared for the first time via a simple silica template method.The 2-5 nm sulfur nan ocrystals being restricted in the cha nnel of the hollow porous carb on spheres are un der a strong compressive stress,which was further con firmed by high-resoluti on tran smissi on electr on microscopy(HRTEM)and GPA.The HPCS-S nano crystals show better con ductivity tha n amorphous sulfur clusters because of the reducti on of the steric hindrance which efficie ntly promotes the electron transportation.Consequently,the higher activity and selectivity towards the 2e^oxygen reduction reaction(ORR)to H2O2 in alkaline solution was obtained.The H2O2 selectivity rises from 20%to over 70%after the sulfur addition and the H2O2 production rate achieves 183.99 mmol-gcataiyst-1 with the Faradaic efficiency of 70%.Furthermore,performance enhancement mechanism was also investigated using the den sity functional theory(DFT)calculatio ns.After the in troduci ng of sulfur nano crystals,the appeara nee of S-S bond greatly decreases the overpotential compared with S-doping,which results in significant enhancement of the electrocatalytic property.Consequently,the HPCS-S can be an efficient H2O2 production electrocatalyst in alkaline solution. | Guanyu Chen Jiwei Liu Qingqing Li Pengfei Guan Xuefeng Yu Linshen Xing Jie Zhang Renchao Che | 2019 | Nano Research2019，12，10： | 6 |
| 4 | Advances in lithium niobate photonics:development status and perspectives显示文摘Lithium niobate(LN)has experienced significant developments during past decades due to its versatile properties,especially its large electro-optic(EO)coefficient.For example,bulk LN-based modulators with high speeds and a superior linearity are widely used in typical fiber-optic communication systems.However,with everincreasing demands for signal transmission capacity,the high power and large size of bulk LN-based devices pose great challenges,especially when one of its counterparts,integrated silicon photonics,has experienced dramatic developments in recent decades.Not long ago,high-quality thin-film LN on insulator(LNOI)became commercially available,which has paved the way for integrated LN photonics and opened a hot research area of LN photonics devices.LNOI allows a large refractive index contrast,thus light can be confined within a more compact structure.Together with other properties of LN,such as nonlinear/acousto-optic/pyroelectric effects,various kinds of high-performance integrated LN devices can be demonstrated.A comprehensive summary of advances in LN photonics is provided.As LN photonics has experienced several decades of development,our review includes some of the typical bulk LN devices as well as recently developed thin film LN devices.In this way,readers may be inspired by a complete picture of the evolution of this technology.We first introduce the basic material properties of LN and several key processing technologies for fabricating photonics devices.After that,various kinds of functional devices based on different effects are summarized.Finally,we give a short summary and perspective of LN photonics.We hope this review can give readers more insight into recent advances in LN photonics and contribute to the further development of LN related research. | Guanyu Chen Nanxi Li Jun Da Ng Hong-Lin Lin Yanyan Zhou Yuan Hsing Fu Lennon Yao Ting Lee Yu Yu Ai-Qun Liu Aaron J.Danner | 2022 | Advanced Photonics2022，4，3： | 6 |
| 5 | Hierarchical coupling effect in hollow Ni/NiFe2O4-CNTs microsphere via spray-drying for enhanced oxygen evolution electrocatalysis显示文摘Design and fabrication of cost-effective transition metal and their oxides-based nanocomposites are of paramount significance for metal-air batteries and water-splitting.However,the traditional optimized designs for nanostructure are complicated,low-efficient and underperform for wide-scale applications.Herein,a novel hierarchical framework of hollow Ni/NiFe2O4-CNTs compositemicrosphere forcibly-assembled by zero-dimensional(OD)Ni/NiFo204 nanoparticle(<16 nm)and one-dimensional(1D)self-supporting CNTs was fabricated successfully.Benefitted from the unique nanostructure,such monohybrids can achieve remarkable oxygen evolution reaction(OER)performance in alkaline media with a low overpotential and superior durability,which exceeds most of the commercial catalysts based on IrO/RuO2 or other non-noble metal nanomaterials.The enhanced OER performance of Ni/NiFe2OA-CNTs composite is mainly ascribed to the increased catalytic activity and the optimized conductivity induced by the effects of strong hierarchical coupling and charge transfers between CNTs and Ni/NiFe204 nanoparticles.These effects are greatly boosted by the polarized heterojunction interfaces confirmed by electron holography.The density functional theory(DFT)calculation indicates the epitaxial Ni further enriches the intrinsic electrons contents of NiFe204 and thus accelerates absorption/desorption kinetics of OER intermediates.This work hereby paves a facile route to construct the hollow composite microsphere with excellent OER electrocatalytic activity based on non-noble metal oxide/CNTs. | Xuefeng Yu Guanyu Chen Yizhe Wang Jiwei Liu Ke Pei Yunhao Zhao Wenbin You Lei Wang Jie Zhang Linshen Xing Jingjun Ding Guangzhou Ding Min Wang Renchao Che | 2020 | Nano Research2020，13，2： | 3 |
| 6 | Neural-network-based Power System State Estimation with Extended Observability显示文摘This paper proposes a neural-network-based state estimation(NNSE)method that aims to achieve higher time efficiency,improved robustness against noise,and extended observability when compared with the conventional weighted least squares(WLS)state estimation method.NNSE consists of two parts,the linear state estimation neural network(LSE-net)and the unobservable state estimation neural network(USE-net).The LSE-net functions as an adaptive approximator of linear state estimation(LSE)equations to estimate the nominally observable states.The inputs of LSE-net are the vectors of synchrophasors while the outputs are the estimated states.The USE-net operates as the complementary estimator on the nominally unobservable states.The inputs are the estimated observable states from LSE-net while the outputs are the estimation of nominally unobservable states.USE-net is trained off-line to approximate the veiled relationship between observable states and unobservable states.Two test cases are conducted to validate the performance of the proposed approach.The first case,which is based on the IEEE 118-bus system,shows the comprehensive performance of convergence,accuracy,and robustness of the proposed approach.The second case study adopts real-world synchrophasor measurements,and is based on the Jiangsu power grid,which is one of the largest provincial power systems in China. | Guanyu Tian Yingzhong Gu Di Shi Jing Fu Zhe Yu Qun Zhou | 2021 | Journal of Modern Power Systems and Clean Energy2021，9，5： | 3 |
| 7 | Impacts of El Nino on the somatic condition of Humboldt squid based on the beak morphology显示文摘The Humboldt squid Dosidicus gigas has a short life span, and environmental variability plays a significant role in regulating its population dynamics and distribution. An analysis of 1 096 samples of D. gigas collected by the Chinese commercial fishing vessels during 2013, 2014, and 2016 off the Peruvian Exclusive Economic Zone, was conducted to evaluate the impacts of El Nino events on the somatic condition of D. gigas. This study indicates that the slopes of all beak variables in relation to mantle length (ML) for females were greater than those of males during 2013, 2014, and 2016, and slopes of the upper crest length and the lower rostrum length significantly differed between females and males in 2013 (P<0.05). Variation in the slopes for beak variables among years was studied;no significant difference was observed (ANCOVA, P>0.05). The Fulton's condition coefficients (K) of females and males in 2013 and 2014 were significantly greater than those in 2016 (P<0.01). The K values of females were greater than those of males in 2013, 2014, and 2016, and K values significantly differed between females and males in 2013. In normal years, the chlorophyll a (Chl a) concentration showed an N-shaped variability from January to December. However, in the El Nino period, it tended to weaken the upwelling coupled with warm and low Chl a concentration waters. We suggest that the poor somatic condition of D. gigas during the El Nino year was resulted from the low Chl a concentration in the waters, and the abundance of D. gigas would decrease due to the unfavourable environment and the lack of prey items in the El Nino year. | HU Guanyu YU Wei LI Bai HAN Dongyan CHEN Xinjun CHEN Yong LI Jianhua | 2019 | Journal of Oceanology and Limnology2019，37，4： | 1 |
| 8 | On-chip WDM mode-di- vision multiplexing interconnection with optional demodulation function 显示文摘 | YE Mengyuan YU Yu CHEN Guanyu | 2015 | OPTICS EXPRESS2015，23，32： | 1 |
| 9 | Crystal- line conjugated polymer containing fused 2,5-di(thiophen-2- yl) thieno2,3-b thiophene and thieno3,4-c pyrrole-4,6-di- one units for bulk heterojunction solar cells 显示文摘 | Chen Guanyu Yu Hsin Cheng Yi Jen Chou | 2011 | Chem Com- mun2011，47，： | 1 |
| 10 | Enhanced Denoising Autoencoder-aided Bad Data Filtering for Synchrophasor-based State Estimation显示文摘Due to its high accuracy and ease of calculation,synchrophasor-based linear state estimation(LSE)has attracted a lot of attention in the last decade and has formed the cornerstone of many wide area monitor system(WAMS)applications.However,an increasing number of data quality concerns have been reported,among which bad data can significantly undermine the performance of LSE and many other WAMS applications it supports.Bad data filtering can be difficult in practice due to a variety of issues such as limited processing time,non-uniform and changing patterns,and etc.To pre-process phasor measurement unit(PMU)measurements for LSE,we propose an improved denoising autoencoder(DA)-aided bad data filtering strategy in this paper.Bad data is first identified by the classifier module of the proposed DA and then recovered by the autoencoder module.Two characteristics distinguish the proposed methodology:1)The approach is lightweight and can be implemented at individual PMU level to achieve maximum parallelism and high efficiency,making it suited for real-time processing;2)the system not only identifies bad data but also recovers it,especially for critical measurements.We use numerical experiments employing both simulated and real-world phasor data to validate and illustrate the effectiveness of the proposed method. | Guanyu Tian Yingzhong Gu Zhe Yu Qibing Zhang Di Shi Qun Zhou Zhiwei Wang | 2022 | CSEE Journal of Power and Energy Systems2022，8，2： | 0 |
| 11 | LotusSQL:SQL Engine for High-Performance Big Data Systems显示文摘In recent years,Apache Spark has become the de facto standard for big data processing.SparkSQL is a module offering support for relational analysis on Spark with Structured Query Language(SQL).SparkSQL provides convenient data processing interfaces.Despite its efficient optimizer,SparkSQL still suffers from the inefficiency of Spark resulting from Java virtual machine and the unnecessary data serialization and deserialization.Adopting native languages such as C++could help to avoid such bottlenecks.Benefiting from a bare-metal runtime environment and template usage,systems with C++interfaces usually achieve superior performance.However,the complexity of native languages also increases the required programming and debugging efforts.In this work,we present LotusSQL,an engine to provide SQL support for dataset abstraction on a native backend Lotus.We employ a convenient SQL processing framework to deal with frontend jobs.Advanced query optimization technologies are added to improve the quality of execution plans.Above the storage design and user interface of the compute engine,LotusSQL implements a set of structured dataset operations with high efficiency and integrates them with the frontend.Evaluation results show that LotusSQL achieves a speedup of up to 9 in certain queries and outperforms Spark SQL in a standard query benchmark by more than 2 on average. | Xiaohan Li Bowen Yu Guanyu Feng Haojie Wang Wenguang Chen | 2021 | Big Data Mining and Analytics2021，4，4： | 0 |
| 12 | Clipping discrete multi-tone for peak-power-constraint IM/DD optical systems显示文摘High peak-to-average power ratio(PAPR)is a major drawback of discrete multi-tone(DMT)in peak-power-constraint(PPC)intensity-modulation/direct-detection(IM/DD)optical systems.Symmetric clipping operation is a frequently-used and practical method for mitigating the PAPR of the DMT signal.However,clipping noise is inevitably induced by the symmetric clipping operation,thereby deteriorating the system’s performance.In this paper,we study the statistical characteristics of the clipping noise in detail.Depending on the statistical characteristics,clipping-noise-cancellation(CNC)algorithms are proposed to mitigate the clipping noise in the clipping DMT systems.Theoretically,the clipping noise can be completely removed using the low-density-parity-check-assisted CNC algorithm(LDPC-assisted CNC algorithm).To verify the superiority of the clipping DMT with the CNC algorithm,we conduct an experiment with a50 Gb/s DMT system using 10G-class commercial devices for the scenario of PPC passive optical networks.The receiver sensitivity of the clipping DMT system with the CNC algorithm can reach-24 d Bm at a 20%soft-decision forward-error-correction limit,which is 2.5 d B higher than that of the DMT system without clipping operation.The clipping operation and the CNC algorithm are added after the original digital signal processing,thereby demonstrating the potential to efectively overcome the drawback in DMT signals and other high-PAPR signals. | Ji ZHOU Liangchuan LI Jiale HE Xiaofeng LU Yu BO Guanyu WANG Yuanda HUANG Gengchen LIU Yanzhao LU Shecheng GAO Yuanhua FENG Shancheng ZHAO Weiping LIU Changyuan YU Zhaohui LI | 2023 | Science China(Information Sciences)2023，66，5： | 0 |
| 13 | Integrated proteomic and phosphoproteomic data-independent acquisition data evaluate the personalized drug responses of primary and metastatic tumors in colorectal cancer显示文摘Mass spectrometry(MS)-based proteomics and phosphoproteomics are powerful methods to study the biological mechanisms,diagnostic biomarkers,prognostic analysis,and drug therapy of tumors.Dataindependent acquisition(DIA)mode is considered to perform better than data-dependent acquisition(DDA)mode in terms of quantitative reproducibility,specificity,accuracy,and identification of lowabundance proteins.Mini patient derived xenograft(MiniPDX)model is an effective model to assess the response to antineoplastic drugs in vivo and is helpful for the precise treatment of cancer patients.Kinases are favorable spots for tumor-targeted drugs,and their functional completion relies on signaling pathways through phosphorylating downstream substrates.Kinase-phosphorylation networks or edge interactions are considered more credible and permanent for characterizing complex diseases.Here,we provide a workflow for personalized drug response assessment in primary and metastatic colorectal cancer(CRC)tumors using DIA proteomic data,DIA phosphoproteomic data,and MiniPDX models.Three kinase inhibitors,afatinib,gefitinib,and regorafenib,are tested pharmacologically.The process mainly includes the following steps:clinical tissue collection,sample preparation,hybrid spectral libraries establishment,MS data acquisition,kinase-substrate network construction,in vivo drug test,and elastic regression modeling.Our protocol gives a more direct data basis for individual drug responses,and will improve the selection of treatment strategies for patients without the druggable mutation. | Xumiao Li Yiming Huang Kuo Zheng Guanyu Yu Qinqin Wang Lei Gu Jingquan Li Hui Wang Wei Zhang Yidi Sun Chen Li | 2023 | Biophysics Reports2023，9，2： | 0 |
| 14 | 外科手术加动脉栓塞治疗直肠Castleman病:病例报告和文献复习显示文摘A 23-year-old male patient was diagnosed as having a hypervascular pelvic mass by ultrasonography and magnetic resonance examination.A pathology puncture showed vitreous vascular Castleman’s disease.Because of concerns about tumor blood supply,embolization under digital subtraction angiography(DSA)was performed on the artery of the pelvic tumor before resection of the mass and surrounding rectum.Castleman’s disease of pelvic lymph node(mixed type,mainly hyaline vascular type)was confirmed pathologically from postoperative biopsy.Embolization of the blood-supply artery of a hypervascular mass should be considered before surgery is performed. | Guanyu Yu Fuao Cao Haifeng Gong Peng Liu Ge Sun Wei Zhang | 2019 | Gastroenterology Report2019，7，2： | 0 |
| 15 | Experimental study on the anti-inflammatory effect of Mongolian medicine Sendeng Decoction on mice with psoriasis显示文摘Psoriasis is a chronic papulosquamous skin disease occurring worldwide caused by varieties of factors.The recipe of Mongolian medicine Sendeng Decoction was recorded in“The Pleasure of the Viewer”compiled by Jigwood Danjinzhamusu.Mongolian medicine clinical experience proved that Sendeng Decoction had good antipyretic,analgesic,anti-inflammatory and anti-rheumatism effect.However,the mechanism of this drug in psoriasis is still unclear.In this study,imiquimod cream(IMQ)was used to construct psoriasis model in 8-week-old male BALB/c mice,and Sendeng Decoction was used to treat the mice with psoriasis.The results showed that PASI score and spleen index decreased after Sendeng Decoction treatment.Meanwhile,HE staining showed that the pathological structure was improved,flow cytometry results showed that the proportion of Treg cells increased and Th17 cells decreased,and results of Western blot showed that the level of p38 MAPK,p-p38 MAPK,p-STAT3 and p-JAK1 decreased observably.More importantly,the above results were most significant in Sendeng's M group,which was similar to MTX group.Our results indicated that Mongolian medicine Sendeng Decoction can effectively relieve the symptoms of psoriasis mice induced by IMQ,mainly by regulating the ratio of Th17/Treg cells through the JAK/STAT and p38 MAPK pathways to reduce the inflammatory state in mice,thereby improving the symptoms of dermatitis. | Li Sun Guanyu Yu Huiming Jiang Kun Shi Doudou Huang Hashen Bao Yanping Huang Limuge Che Meijuan Chen | 2022 | Medicine in Novel Technology and Devices2022，，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员