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| 1 | Energy sources identify metabolic phenotypes n pancreatic cancer显示文摘 | Chen Liang Yi Qin Bo Zhang Shunrong Ji Si Shi Wenyan Xu Jiang Liu Jinfeng Xiang Dingkong Liang Qiangsheng Hu Liang Liu Chen Liu Guopei Luo Quanxing Ni Jin xu Xianjun Yu | 2016 | Acta Biochimica et Biophysica Sinica2016，48，11： | 3 |
| 2 | Blood Neutrophil–Lymphocyte Ratio Predicts Survival in Patients with Advanced Pancreatic Cancer Treated with Chemotherapy显示文摘 | Guopei Luo Meng Guo Zuqiang Liu Zhiwen Xiao Kaizhou Jin Jiang Long Liang Liu Chen Liu Jin Xu Quanxing Ni Xianjun Yu | 2015 | Annals of Surgical Oncology2015，，： | 1 |
| 3 | Molecular Mechanism Underlying Lymphatic Metastasis in Pancreatic Cancer显示文摘 | Zhiwen Xiao Guopei Luo Chen Liu Chuntao Wu Liang Liu Zuqiang Liu Quanxing Ni Jiang Long Xianjun Yu Sonshin Takao | 2014 | BioMed Research International2014，，： | 1 |
| 4 | Molecular Mechanism Underlying Lymphatic Metastasis in Pancreatic Cancer显示文摘 | Zhiwen Xiao Guopei Luo Chen Liu Chuntao Wu Liang Liu Zuqiang Liu Quanxing Ni Jiang Long Xianjun Yu Sonshin Takao | 2014 | BioMed Research International2014，，： | 1 |
| 5 | Highly lymphatic metastatic pancreatic cancer cells possess stem cell-likeproperties显示文摘 | Guopei Luo Jiang Long Xiaobo Cui Zhiwen Xiao Zuqiang Liu Si Shi Liang Liu Chen Liu Jin Xu Min Li Xianjun Yu | 2013 | International Journal of Oncology2013，，： | 1 |
| 6 | Glutamine deprivation induces ferroptosis in pancreatic cancer cells显示文摘Ferroptosis is a type of programmed cell death closely related to amino acid metabolism.Pancreatic cancer cells have a strong dependence on glutamine,which serves as a carbon and nitrogen substrate to sustain rapid growth.Glutamine also aids in self-protection mechanisms.However,the effect of glutamine on ferroptosis in pancreatic cancer remains largely unknown.Here,we aim to explore the association between ferroptosis and glutamine deprivation in pancreatic cancer.The growth of pancreatic cancer cells in culture media with or without glutamine is evaluated using Cell Counting Kit-8.Reactive oxygen species(ROS)are measured by 2′,7′-dichlorodihydrofluorescein diacetate staining.Ferroptosis is assessed by BODIPY-C11 dye using confocal microscopy and flow cytometry.Amino acid concentrations are measured using ultrahigh-performance liquid chromatography-tandem mass spectrometry.Isotope-labelled metabolic flux analysis is performed to track the metabolic flow of glutamine.Additionally,RNA sequencing is performed to analyse the genetic alterations.Glutamine deprivation inhibits pancreatic cancer growth and induces ferroptosis both in vitro and in vivo.Additionally,glutamine decreases ROS formation via glutathione production in pancreatic cancer cells.Interestingly,glutamine inhibitors(diazooxonorleucine and azaserine)promotes ROS formation and ferroptosis in pancreatic cancer cells.Furthermore,ferrostatin,a ferroptosis inhibitor,rescues ferroptosis in pancreatic cancer cells.Glutamine deprivation leads to changes in molecular pathways,including cytokine-cytokine receptor interaction pathways(CCL5,CCR4,LTA,CXCR4,IL-6R,and IL-7R).Thus,exogenous glutamine is required for the detoxification of ROS in pancreatic cancer cells,thereby preventing ferroptosis. | Zhiwen Xiao Shengming Deng He Liu Ruijie Wang Yu Liu Zhengjie Dai Wenchao Gu Quanxing Ni Xianjun Yu Chen Liu Guopei Luo | 2023 | Acta Biochimica et Biophysica Sinica2023，55，8： | 0 |
| 7 | HNF-1a promotes pancreatic cancer growth and apoptosis resistance via its target gene PKLR显示文摘Pancreatic ductal adenocarcinoma is one of the deadliest malignant tumors,and many genes play important roles in its development.The hepatocyte nuclear factor-1a(HNF-1a)gene encodes HNF-1a,which is a transcriptional activator.HNF-1a regulates the tissue-specific expression of multiple genes,especially in pancreatic islet cells and in the liver.However,the role of the HNF-1a gene in the development of pancreatic cancer is still unclear.Here,we used immunohistochemical staining and real-time PCR to analyze HNF-1a expression in pancreatic cancer tissue.Stable cell lines with HNF-1a knockdown or overexpression were established to analyze the role of HNF-1a in pancreatic cancer cell proliferation and apoptosis by colony formation assay and flow cytometry.We also analyzed the L-type pyruvate kinase(PKLR)promoter sequence to identify the regulatory effect of HNF-1a on PKLR transcription and confirmed the HNF-1a binding site in the PKLR promoter via a chromatin immunoprecipitation assay.HNF-1a was found to be overexpressed in pancreatic cancer and promoted proliferation while inhibiting apoptosis in pancreatic cancer cells.PKLR was identified as the downstream target gene of HNF-1a and binding of HNF-1a at two sites in PKLR(-1931/-1926 and-966/-961)regulated PKLR transcription.In conclusion,HNF-1a is overexpressed in pancreatic cancer,and the transcription factor HNF-1a can promote pancreatic cancer growth and apoptosis resistance via its target gene PKLR. | Zhiyao Fan Kun Fan Shengming Deng Yitao Gong Yunzhen Qian Qiuyi Huang Chao Yang He Cheng Kaizhou Jin Guopei Luo Chen Liu Xianjun Yu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，3： | 0 |
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