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| 1 | LncRNA SNHG5/miR-26a/SOX2 signal axis enhances proliferation of chondrocyte in osteoarthritis显示文摘Chondrocyte 在骨关节炎(OA ) 涉及关节的破坏病人。这研究的目的是探索小核仁的 RNA 主机基因 5 的表达式水平(SNHG5 ) 并且在 chondrocyte 评估它的函数。在我们的当前的学习, SNHG5 的表示层次, miR-26a,和在 17 关节的软骨纸巾并且在 non-OA 的 SOX2,组被即时量的反向抄写的聚合酶链反应估计。结果证明 SNHG5 和 SOX2 的层次是显著地在 OA 纸巾的 downregulated,当 miR-26a 的水平是 upregulated 时。MTT,殖民地形成和房间 transwell 试金被执行在 CHON-001 房间在房间生存能力,生长能力,和迁居能力上估计 SNHG5 的功能。SNHG5 能支持 chondrocyte 房间增长和移植,这被发现。在 SNHG5 和 miR-26a 之间的关系被证实由裂开并且酶记者试金。SOX2 被酶记者试金作为 miR-26a 的目标基因识别。营救试金被使用在 SNHG5, miR-26a,和 SOX2 之中验证关系。我们的当前的学习证明 SNHG5 通过作为 ceRNA 工作因此,竞争地擦掉 miR-26a 调整 SOX2 的表示涉及 OA 的机制。 | Huijun Shen Yue Wang Wudan Shi Guoxun Sun Luojia Hong Ying Zhang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，2： | 18 |
| 2 | Electrochemical behavior of zirconium in molten NaCl-KCl-K_2ZrF_6 system显示文摘The electrochemical reduction of Zr4+(complex) ions in NaCl-KCl-K2ZrF6 molten salt on Pt electrode was investigated using cyclic voltammetry and square wave voltammetry at 1023 K.Two cathodic reduction peaks related to Zr4+/Zr2+ and Zr2+/Zr steps were observed in the cyclic voltammograms.The result was also confirmed by square wave voltammetry.The diffusion coefficient of Zr4+(complex) ions at 1023 K in NaCl-KCl-K2ZrF6 melt,measured by cyclic voltammetry,is about 4.22×10-6 cm2/s.The characterization of the deposits obtained by potentiostatic electrolysis at different potentials was investigated by XRD,and the results were well consistent with the electrochemical reduction mechanism of Zr4+(complex) ions. | WU Yanke XU Zhigao CHEN Song WANG Lijun LI Guoxun | 2011 | Rare Metals2011，30，1： | 16 |
| 3 | PGC-1α induces apoptosis in human epithelial ovarian cancer cells through a PPARy-dependent pathway显示文摘Peroxisome 激活 proliferator 的受体鲸鱼群妈(PPAR γ) coactivator-1 高山哈(PGC-1 α) coactivates 多重抄写因素并且调整几个代谢过程。Thecurrent 学习在人的上皮的卵巢的癌症房间在 apoptosis 的正式就职调查了 PGC-1 α的角色。在人的卵巢和人的卵巢的上皮的肿瘤之间的 PGC-1 α信使 rna 水平被量的 RT-PCR 检验。更少的 PGC-1 α表示与正常卵巢相比在恶意的肿瘤的表面上皮被发现。在人的上皮的卵巢的癌症房间线 Ho-8910 的 PGC-1 α的 Overexpression 通过 Bcl-2 和 Bax 表示的协调规定导致了房间 apoptosis。Microarray 分析证实 PGC-1 α戏剧性地在 Ho-8910 房间影响了 apoptosis 相关的基因。 Mitochondrial 功能的试金证明 apoptosis 的正式就职通过由细胞色素 c.Furthermore 的版本的终端阶段,导致的 apoptosis 部分是,然而并非完全,由 PPAR γa ntagonist ( GW9662 )堵住了的 PGC-1 α-,并且由 siRNA 的 PPAR γ 表示的抑制也在 Ho-8910 房间禁止了 PGC-1 α- inducedapoptosis 。这些数据建议 PGC-1 α通过 aPPAR γ - 依赖者小径施加了它的效果。我们的调查结果显示 PGC-1 α涉及 apoptotic signaltransduction 小径, PGC-1 α的 down 规定可以是在支持上皮的卵巢的癌症生长和前进的一个关键点。 | Yan Zhang Yi Ba Chang Liu Guoxun Sun Li Ding Songyuan Gao Jihui Hao Zhentao Yu Junfeng Zhang Ke Zen Zhongsheng Tong Yang Xiang Chen-Yu Zhang | 2007 | Cell Research2007，17，4： | 14 |
| 4 | Insect Cell Culture and Biotechnology显示文摘The continued development of new cell culture technology is essential for the future growth and application of insect cell and baculovirus biotechnology. The use of cell lines for academic research and for commercial applications is currently dominated by two cell lines; the Spodoptera frugiperda line, SF21 (and its clonal isolate, SF9), and the Trichoplusia ni line, BTI 5B1-4, commercially known as High Five cells. The long perceived prediction that the immense potential application of the baculovirus-insect cell system, as a tool in cell and molecular biology, agriculture, and animal health, has been achieved. The versatility and recent applications of this popular expression system has been demonstrated by both academia and industry and it is clear that this cell-based system has been widely accepted for biotechnological applications. Numerous small to midsize startup biotechnology companies in North America and the Europe are currently using the baculovirus-insect cell technology to produce custom recombinant proteins for research and commercial applications. The recent breakthroughs using the baculovirus-insect cell-based system for the development of several commercial products that will impact animal and human health will further enhance interest in this technology by pharma. Clearly, future progress in novel cell and engineering advances will lead to fundamental scientific discoveries and serve to enhance the utility and applications of this baculovirus-insect cell system. | Robert R. Granados Guoxun Li G. W. Blissard | 2007 | 中国病毒学2007，22，2： | 12 |
| 5 | Effect of electrolysis voltage on electrochemical reduction of titanium oxide to titanium in molten calcium chloride显示文摘The electrochemical reduction of solid TiO2 directly to solid metal is a promising alternative to the current Kroll process. The present work is aimed at studying the effect of electrolysis voltage on the rate of electrochemical reduction. The products of electrochemical reduction of TiO2 and Ti2O were examined using the scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques. The results show that Ti2O was reduced to low valent titanium oxide at 1.5-1.7 V, which was the result of ionization of oxygen. TiO2 and Ti2O were reduced to titanium metal at 2.1-3.1 V, which was the co-action of ionization of oxygen and calciothermic reduction. The oxygen content decreased rapidly with voltage increas-ing from 2.1 to 2.6 V, while it changed little from 2.6 to 3.1 V. The optimized cell voltage was 2.6-3.1 V. | LIU Meifeng LU Shigang KAN Surong LI Guoxun | 2007 | Rare Metals2007，26，6： | 7 |
| 6 | Construction and characteristics of a transformed lepidopteran cell clone expressing baculovirus p35显示文摘A transformed cell line was constructed fromMythimna separata cells Ms7311 by lipofection methodTMs7311 cells were generated using a double selection tech-nique involving a selection in the antibiotic Zeocin, followedby a second round of selection by exhibiting cell characteri-zation. A cell clone expressing p35 was obtained with highlevel of AcMNPV and recombinant proteins. Compared withwild type Ms7311 cells, the cell clone showed increased resis-tance to Actinamycin D-induced apoptosis and a profoundresistance to nutrient development (PBS). When the cellclone was infected with recombinant baculoviruses express-ing secreted alkaline phosphatase (SEAP) and β-galactosi-dase, expression of the recombinant proteins from TMs7311cells exceeded that from parental Ms7311 cells. Production ofbudded virus and occlusion body was significantly higherthan that from parental cells Ms7311. | ZHENG Gulling LI Changyou LI Guoxun WANG Ping Robert R. Granados | 2005 | Chinese Science Bulletin2005，50，23： | 6 |
| 7 | A more consistent intraluminal rhesus monkey model of ischemic stroke显示文摘Endovascular surgery is advantageous in experimentally induced ischemic stroke because it causes fewer cranial traumatic lesions than invasive surgery and can closely mimic the pathophysiology in stroke patients. However, the outcomes are highly variable, which limits the accuracy of evaluations of ischemic stroke studies. In this study, eight healthy adult rhesus monkeys were randomized into two groups with four monkeys in each group: middle cerebral artery occlusion at origin segment(M1) and middle cerebral artery occlusion at M2 segment. The blood flow in the middle cerebral artery was blocked completely for 2 hours using the endovascular microcoil placement technique(1 mm × 10 cm)(undetachable), to establish a model of cerebral ischemia. The microcoil was withdrawn and the middle cerebral artery blood flow was restored. A reversible middle cerebral artery occlusion model was identified by hematoxylin-eosin staining, digital subtraction angiography, magnetic resonance angiography, magnetic resonance imaging, and neurological evaluation. The results showed that the middle cerebral artery occlusion model was successfully established in eight adult healthy rhesus monkeys, and ischemic lesions were apparent in the brain tissue of rhesus monkeys at 24 hours after occlusion. The rhesus monkeys had symptoms of neurological deficits. Compared with the M1 occlusion group, the M2 occlusion group had lower infarction volume and higher neurological scores. These experimental findings indicate that reversible middle cerebral artery occlusion can be produced with the endovascular microcoil technique in rhesus monkeys. The M2 occluded model had less infarction and less neurological impairment, which offers the potential for application in the field of brain injury research. | Bo Zhao Guowei Shang Jian Chen Xiaokun Geng Xin Ye Guoxun Xu Ju Wang Jiasheng Zheng Hongjun Li Fauzia Akbary Shengli Li Jing Lu Feng Ling Xunming Ji | 2014 | Neural Regeneration Research2014，9，23： | 6 |
| 8 | A novel class of apical sodium–dependent bile salt transporter inhibitors:1-(2,4-bifluorophenyl)-7-dialkylamino-1,8-naphthyridine-3-carboxamides显示文摘The apical sodium-dependent bile acid transporter(ASBT) is the main transporter to promote re-absorption of bile acids from the intestinal tract into the enterohepatic circulation.Inhibition of ASBT could increase the excretion of bile acids,thus increasing bile acid synthesis and consequently cholesterol consumption.Therefore,ASBT is an attractive target for developing new cholesterol-lowering drugs.In this report,a series of 1-(2,4-bifluorophenyl)-7-dialkylamino-1,8-naphthyridine-3-carboxamides were designed as inhibitors of ASBT.Most of them demonstrated potency against ASBT transport of bile acids.In particular,compound 4a_1 was found to have the best activity,resulting in 80.1%inhibition of ASBT at10μmol/L. | Hongtao Liu Guoxun Pang Jinfeng Ren Yue Zhao Juxian Wang | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 2 |
| 9 | A new kind of magnetic targeting induction heating drug carrier and its physical and biological properties显示文摘Nano-carbon and iron composite―carbon-coated iron nanoparticles (CCINs) produced by carbon arc method can be used as a new kind of magnetic targeting induction heating drug carrier for cancer therapy. The structure and morphology of CCINs are studied by X-ray diffraction (XRD) and transmission electron microscope (TEM). Mossbauer spectra of these nanoparticles show that they contain only iron and carbon, without ferric carbide and ferric oxide. CCINs can be used as the magnetic drug carrier, with the effect of targeting magnetic induction heating in its inner core and higher drug adsorption in its nano-carbon shell outside because of its high specific surface area. CCINs can absorb Epirubicin (EPI) of 160 μg/mg measured by an optical spectrometer. In acute toxicity experiment with mice, the median lethal dose (LD50) of EPI is 16.9 mg/kg, while that of EPI-CCINs mixture is 20.7 mg/kg and none of the mice died after pure CCINs medication. The results show that pure CCINs belong to non-toxic grade and EPI delivery in mixture with CCINs can reduce its acute toxicity in mice. The magnetic properties of CCINs and their magnetic induction heating are investigated. The iron nanoparticle in its inner core has better magnetism with a good effect on targeting magnetic induction heating. When the CCINs are mixed with physiological salt water and are injected uniformly in pig's liver, the temperature goes up to 48℃. While in the case that CCINs are filled in a certain section of pig's liver, the temperature goes up to 52℃. In both cases the temperature is high enough to kill the cancer cell. CCINs have potential applications in cancer therapy. | ZHANG HaiYan CHEN YiMing ZHENG Yun LAO XiangMing ZENG GuoXun PANG JinShan WU QiGuang LI JinQing | 2009 | Science China(Technological Sciences)2009，52，10： | 2 |
| 10 | A novel chitin-binding protein identified from the peritrophic membrane of the cabbage looper, Trichoplusia ni显示文摘 | Wei Guo Guoxun Li Yi Pang Ping Wang | 2005 | Insect Biochemistry and Molecular Biology2005，，11： | 2 |
| 11 | MATHEMATICAL MODELLING OF BUBBLE DRIVENFLOWS IN A NOVEL ALUMINUM ELECTROLYSIS CELL显示文摘MATHEMATICALMODELLINGOFBUBBLEDRIVENFLOWSINANOVELALUMINUMELECTROLYSISCELLGaoZhaosheng;LiGuoxun;ShenJianyun(BeijingGeneralResea... | Gao Zhaosheng Li Guoxun Shen Jianyun(Beijing General Research institute for Non-ferrous Metals, Beijing 100088) | 1996 | 中国有色金属学会会刊:英文版1996，6，2： | 2 |
| 12 | Unified optimal design of external parallel indexing cam mechanism显示文摘 | Peng Guoxun Xiao Zhengyang | 1988 | MMT1988，23，4： | 2 |
| 13 | User prefer-ences based software defect detection althorithums selec-tion using MCDM显示文摘 | Yi Peng Guoxun Wang and Honggang Wang | 2012 | Information Sciences2012，191，： | 1 |
| 14 | Unsupervised content-preserving transformation for optical microscopy显示文摘The development of deep learning and open access to a substantial collection of imaging data together provide a potential solution for computational image transformation,which is gradually changing the landscape of optical imaging and biomedical research.However,current implementations of deep learning usually operate in a supervised manner,and their reliance on laborious and error-prone data annotation procedures remains a barrier to more general applicability.Here,we propose an unsupervised image transformation to facilitate the utilization of deep learning for optical microscopy,even in some cases in which supervised models cannot be applied.Through the introduction of a saliency constraint,the unsupervised model,named Unsupervised content-preserving Transformation for Optical Microscopy(UTOM);can learn the mapping between two image domains without requiring paired training data while avoiding distortions of the image content.UTOM shows promising performance in a wide range of biomedical image transformation tasks,including in silico histological staining,fluorescence image restoration,and virtual fluorescence labeling.Quantitative evaluations reveal that UTOM achieves stable and high-fidelity image transformations across different imaging conditions and modalities.We anticipate that our framework will encourage a paradigm shift in training neural networks and enable more applications of artificial intelligence in biomedical imaging. | Xinyang Li Guoxun Zhang Hui Qiao Feng Bao Yue Deng Jiamin Wu Yangfan He Jingping Yun Xing Lin Hao Xie Haoqian Wang Qionghai Dai | 2021 | Light(Science & Applications)2021，10，3： | 1 |
| 15 | Grey Relational Analyzing Between Human Qulity and Accident显示文摘 | Jing Guoxun Wu Weidong Feng Changgen | 2000 | The Journal of Grey System2000，，3： | 1 |
| 16 | The preliminary discussing of Qi transfor- mation显示文摘 | Gao Guoxun | 1982 | Journal of Jianghan University(Natural Science Edition)1982，9，1： | 1 |
| 17 | Effect of adding Y2O3 in cermet electrodes on the electrical conductivities显示文摘 | Wang Changfu Li Guoxun Qu Shuling | 1992 | Rare Metals1992，11，4： | 1 |
| 18 | An over-sampling expert system for learning from imbalaneed data sets显示文摘 | Han Hui Wang Wenyuan | 2005 | Neural Networks and Brain2005，1，： | 1 |
| 19 | The thermal properties of controllable diameter carbon nanotubos synthesized by using AB5 alloy of micrometer magnitude es catalyst显示文摘 | YIMING CHEN GUOXUN ZENG | 2007 | Materials Science and Engineering:A2007，464，12： | 1 |
| 20 | Impact of MAPK cascade pathway and P53 pathway upon liver transplant显示文摘 | GONG Niartqiao LI Guoxun XIAO Jiansheng | 2005 | Huazhong Unvi Sci Technol Med Sci2005，25，5： | 1 |
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