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| 1 | A novel Lyn-like protein tyrosine kinase identified in lamprey and its role in immune response显示文摘The avian sarcoma virus transforming gene(Src)kinase family,a type of nonmreceptor protein tyrosine kinases(nrTPKs),has been reported to play roles in cell growth,division,migration,adhesion,the immune response,and signal transduction berween cells[1,2].The Src family kinases can be divided into three subfamilies based on their conserved domains:SrcA subfamily(Sre,Yes,Fyn,and Fgr,SrcB subfamily(Lck,Hck,Blk,and Lyn),and Frk subfamily[3]. | Tingting Zhao Kedi Luo Xin Liu Shanshan Dong Yinglun Han Meng Gou Peng Su Qingwei Li Ting Zhu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 2 |
| 2 | Lamprey serum can kill HeLa and NB4 tumor cells显示文摘 | Yinglun Han Yue Pang Tao Yu Rong Xiao Biyue Shi Peng Su Xin Liu Qingwei Li | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 1 |
| 3 | A novel CD81 homolog identified in lamprey, Lampetra japonica,with roles in the immune response of lamprey VLRB^+lymphocytes显示文摘The cluster of differentiation 81 (CD81),a member of the transmembrane 4 superfamily,is primarily found to be expressed in a wide variety of cells including T and B cells of vertebrates as a critical modulator.In the present study,the open reading frame of a CD81 gene homolog (Lja-CD81) was cloned in lamprey,Lampetra japonica,which is 702 bp long and encodes a protein of 233- amino acids.Although Lja-CD81 seems to be close to CD9 molecules in their full-length sequences,Lja-CD81 possesses higher identity to vertebrates CD81 than to CD9 (including a lamprey CD9)molecules in their large extracellular loops.In addition,it also possesses a myristoylation site (Met-Gly-Val-Glu-Gly-Cys-Leu-Lys)in its N-terminal region which is identical to the N-terminal regions of CD81 molecules.These data suggest that CD9 and CD81 molecules diverged no later than the emergence of jawless vertebrates.The mRNA levels of Lja-CD81 in lymphocytes and supraneural myeloid bodies were up-regulated significantly after stimulation with mixed antigens,and a similar expressional pattern of Lja-CD81 at protein level was also confirmed. Furthermore,Lja-CD81 was found to be co-localized with variable lymphocyte receptor B (VLRB) evenly on the cell membrane of peripheral blood lymphocytes isolated from control group,but they were found to aggregate on one side of the membrane of peripheral blood VLRB+lymphocytes after stimulation with mixed antigens.All these results indicate that the Lja-CD81 identified in lamprey may play an important role in the immune response of lamprey VLRB+lymphocytes. | Wenjing Liang Miceng Gao Xueying Song Yinglun Han Nleng Go Peng Su Qingwei Li Xin Liu | 2018 | Acta Biochimica et Biophysica Sinica2018，50，11： | 1 |
| 4 | A novel BTK-like protein involved in immune response in Lethenteron japonicum显示文摘 | Fenfang Wu Jing Zhao Liyong Chen Xin Liu Peng Su Yinglun Han Bo Feng Qingwei Li | 2012 | Immunology Letters (-)2012，，1： | 1 |
| 5 | HMGB1 is necessary for the development of lamprey显示文摘High mobility group box protein 1(HMGB1)with a highly conserved structure in chromatin is an abundant nonhistone protein,which is involved in nucleosome folding and assembly,chromatin formation,DNA binding,V(D)J recombination,DNA replication,gene transfer,cell differentiation,cell migration,neurotransmitters,cell death,immune response,and other important processes[1–4].So,it has important biological functions in animals.The function of hmgb1 has been extensively studied in jawed vertebrates but little in lampreys.Lampreys are members of the living agnathans,the most basal group of vertebrates.Lamprey is a considerable model organism in vertebrate evolution and developmental biology because of its special phylogenetic position and critical stage closely related to other animals.Many aspects of the hmgb1 in lampreys have not been revealed,such as evolution,distribution,and role.Therefore,in this paper,the evolution of hmgb1 was carried out,and the function of hmgb1 in lampreys was investigated by the morpholine antisense oligonucleotide(MO)technique.The data provide the foundation for the study of origins,evolution,and the function of hmgb1 in a jawless vertebrate.Linking evolution to function can shed more light on the role of the hmgb1 gene involved in development. | Jingjing Lu Jun Li Zihao Yan Xiaokun Wang Feng Lin Qinghua Ma Huaixiu Liu Haoran Guan Yueying Xun Xiaoying Kang Peijie Luan Xiao Feng Yue Pang Qingwei Li Yinglun Han | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 0 |
| 6 | A novel member of lymphocyte-specific protein tyrosine kinase protein identified in lamprey, Lampetrajaponica显示文摘 | Yinglun Han | 2014 | Acta Biochimica et Biophysica Sinica2014，46，9： | 0 |
| 7 | Molecular cloning,expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey,Lampetra japonica显示文摘The Guanine nucleotide exchange factor Vav2(Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases. In the current study, an ortholog(Lj-Vav2) of Vav2 was identified in the lamprey(Lampetra japonica). To elucidate the phylogenetic relationship of Vav2, the metazoan genome databases were analyzed to mine the ortholog of Vav. It was found that Vav2 genes were only existed in vertebrates and Lj-Vav2 was the original one found in agnathans. The evolutionary dynamics of conserved motifs of Vav2 were explored using combined amino acid sequence as markers, and it is revealed that the Calponin homology(CH) domain, Dbl-homologous(DH) domain, Pleckstrin homology(PH) domain, Cysteine-rich(C1)domains, Src homology 3(SH3) domains and Src homology 2(SH2) domain were conserved throughout the Vav2 gene family in vertebrates during gene evolution. Relative quantitative real-time PCR analysis showed that the LjVav2 was distributed in the heart, kidney, supraneural myeloid body, liver, gill and lymphocyte-like cells. The LjVav2 was found to be expressed in these tissues, and the level of which was upregulated in lymphocyte-like cells after the animal was stimulated with LPS. These results indicated that the Lj-Vav2 might be involved in the immune response of lymphocyte-like cells in lamprey. Meanwhile, our findings provided a foundation for further investigation of the function of Lj-Vav2 in the primary vertebrate. | HAN Yinglun LI Jun XU Lei MA Qinghua GOU Meng PANG Yue LIU Xin LI Qingwei | 2017 | Acta Oceanologica Sinica2017，36，12： | 0 |
| 8 | Immune-related gene expression in the early development of lamprey larva显示文摘 | Jun Li Yinglun Han Ting Zhu Yue Pang Qingwei Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，9： | 0 |
| 9 | Target-directed isolation and identification of a serum lectin from lamprey (Lampetra japonica) by chromatographys and MALDI-TOF/TOF显示文摘A 105-k Da polymer lectin was purified from lamprey(Lampetra japonica) serum by chromatography methods including cation ion-exchange chromatography with a SP-Sepharose TM XL column and size exclusion chromatography with a Superdex 200 column. The target fractions were collected according to the direction of hemagglutinating activity. The results revealed that the active fractions could adsorb on SP-Sepharose column and showed a 280 nm UV absorbance peak corresponding to molecular weights of 105 k Da in the following size exclusion chromatography. The target fractions with hemagglutinating activity were further checked by NativePAGE and SDS-PAGE. Two single bands at around 105 k Da and 35 k Da were displayed by two electrophoresis methods respectively, indicating that the protein exists as a trimer in solution. After Native-PAGE and SDS-PAGE,two bands were excised from the gels respectively and further identified by MALDI-TOF/TOF as serum lectin(gi:13094239). The lectin was able to agglutinate rabbit red blood cells(RRBCs) and sheep red blood cells(SRBCs) in vitro. The lectin isolated from lamprey serum in the current study might be helpful for deeply understanding the innate immune molecules dependent immune defence in jawless vertebrates which have been proved recently that they possess a lymphocyte-based system of anticipatory immunity with variable lymphocyte receptors as mediators. | HAN Yinglun GOU Meng SONG Xiaoping SONG Tao SHI Biyue PANG Yue LI Qingwei | 2018 | Acta Oceanologica Sinica2018，37，5： | 0 |
| 10 | A novel member of B-cell linker protein identified in lamprey, Lampetrajaponica显示文摘 | Yinglun Han | 2014 | Acta Biochimica et Biophysica Sinica2014，46，6： | 0 |
| 11 | A novel ITAM-containing immunoglobulin superfamily receptor involved in lamprey VLRB+lymphocyte immune response显示文摘Adaptive immunity is a specific response of lymphocytes under the stimulation of pathogens,which plays a key role in eliminating pathogens and can produce immune memory effect in preventing reinfection[1].The specificity of the immune response in higher vertebrates is based on the huge antigen receptor spectrum composed of major histocompatibility complex(MHC),T cell receptor(TCR),and B cell receptor(BCR)[2].Although the jawless vertebrates lack the typical immune receptors such as TCR,BCR,and MHC,another type of adaptive immune system formed by other ways has been found[3].Pancer et al.[4]found three types of variable lymphocyte receptor(VLR)in the sea lamprey(Petromyzon marinus).The structural diversity of VLRs results in the diversity and combination of many leucine-rich repeat modules so that each lymphocyte produces only one receptor molecule with a specific structure for a specific antigen[5].VLRA and VLRC are found to be similar toαβandγδT cells of higher vertebrates,which are expressed on VLRA+and VLRC+lymphocytes in gills,respectively[6,7].VLRB is specifically expressed on VLRB+cells(accounting for 90%of the total peripheral blood lymphocytes).Its function is similar to B cells of higher vertebrates.It differentiates into plasma-like cells and expresses secretory VLRB tetramer or pentamer antibodies after antigen stimulation[7]. | Xin Liu Sifan Wang Qidi Ma Wenjing Liang Jun Li Ting Zhu Qingwei Li Yinglun Han | 2021 | Acta Biochimica et Biophysica Sinica2021，53，6： | 0 |
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