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| 1 | CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis显示文摘Dear Editor,The CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9)system is revolutionizing genome editing due to its high efficiency,low cost,design simplicity and versatility.However,introduction of a point mutation at a desired position remains a great challenge in plant genome engineering.Currently,point mutation in plants was achieved by incorporating | Yiyu Chen Zhiping Wang Hanwen Ni Yong Xu Qijun Chen Linjian Jiang | 2017 | Science China(Life Sciences)2017，60，5： | 40 |
| 2 | Shoshonitic intrusive suite in SE Guangxi: Petrology and geochemistry显示文摘A NE-direction Mesozoic shoshonitic intrusive s黫te in SE Guangxi has been identified in terms of geological, petrological and geochemical investigations. The shoshonitic intrusives are characterized by enrichment of LILE, HFSE and LREE and no obvious Nb-Ta depletion, similar to those potassic rocks formed in within-plate and rifting environments. Unlike most shoshonitic rocks forming in arc settings, the SE Guangxi shoshonitic intrusives were likely generated during regional lithosphere extension induced by upwelling of asthenosphere mantle. | LI Xianhua ZHOU Hanwen LIU Ying LEE Chi-yu SUN Min CHEN Chenhong | 2000 | Chinese Science Bulletin2000，45，7： | 32 |
| 3 | Simultaneous determination of ten taste and odor compounds in drinking water by solid-phase microextraction combined with gas chromatography-mass spectrometry显示文摘Taste and odor(T&O)problems in drinking water frequently occur because of many compounds present in the water,of which trans-1,10-dimethyl-trans-9-decalol(geosmin)and 2-methylisoborneol(MIB)are well-known.In this study,a fast and efective method was established for simultaneous determination of 10 T&O compounds,including geosmin,MIB,2,4,6-trichloroanisole(TCA),2-methylbenzofuran,2-isopropyl-3-methoxypyrazine(IPMP),2-isobutyl-3-methoxypyrazine(IBMP),cis-3-hexenyl acetate,trans,trans-2,4-heptadienal,trans,cis-2,6-nonadienal,and trans-2-decenal in water samples by headspace solid-phase microextraction(SPME)coupled with gas chromatography-mass spectrometry.An orthogonal array experimental design was used to optimize the efects of SPME fiber,extraction temperature,stirring rate,NaCl content,extraction time,and desorption time.The limits of detection ranged from 0.1 to 73 ng/L were lower than or close to the odor threshold concentrations(OTCs).All the 10 T&O compounds were detected in the 14 water samples including surface water,treatment process water and tap water,taken from a waterworks in Lianyungang City,China.MIB and geosmin were detected in most samples at low concentration.Six T&O compounds(IPMP,IBMP,trans,cis-2,6-nonadienal,2-methylbenzofuran,trans-2-decenal,and TCA)were efectively decreased in water treatment process(sedimentation and filtration)that is diferent from cis-3-hexenyl acetate,MIB and geosmin.It is noted that the TCA concentrations at 15.9–122.3 ng/L and the trans,cis-2,6-nonadienal concentrations at 79.9–190.1 ng/L were over 10 times higher than their OTCs in tap water.The variation of the analytes in the all water samples,especially distribution system indicated that distribution system cannot be ignored as a T&O compounds source. | Xichao Chen Qian Luo Shengguang Yuan Zi Wei Hanwen Song Donghong Wang Zijian Wang | 2013 | Journal of Environmental Sciences2013，25，11： | 21 |
| 4 | High Pressure Metamorphism of Garnet-Amphibole Rocks from Songshugou Area,Eastern Qinling Mountain and Its Tectonic Implication显示文摘Eclogitic garnet-amphibole rocks are scattered around the Songshugou ultramafic bodies in QinlingMountains.Three metamorphic stages are recognized in terms of petrography,mineral chemistry andgeothermobarometry.The first albite-amphibole stage was of greenschist facies metamorphism with typicalmineral assemblage of actinolite+epidote+chlorite+albite;the pressure and temperature conditions wereequal to or lower than500MPa and about400℃.The second prograde eclogitic garnet-amphibole stagewas of amphibole-eclogite facies metamorphism with typical mineral assemblage of garnet+hornblende±clinopyroxene+rutile without phagioclase,the pressure condition was at least1000 -1200MPa and the temper-ature about600℃.The third retrograde epidote-amphibole stage was of epidote-amphibolite facies withassemblage of plagoclase+hornblende+epidote+ilmenite/titanite,the temperature was500-600℃andthe pressure from 800MPa down to500MPa.All three stages took place in one single tectonothermal eventcalled Jimingian movement | Chen Nengsong Yang Yong Zhou Hanwen Lu QiChina University of Geosdences, Wuhan 430074 | 1993 | Journal of Earth Science1993，12，1： | 9 |
| 5 | Remaining useful life prediction for a nonlinear multi-degradation system with public noise显示文摘To predict the remaining useful life(RUL) for a class of nonlinear multi-degradation systems, a method is presented. In the real industrial processes, systems are usually composed by several parts or components, and these parts or components are working in the same environment, thus the degradations of these parts or components will be influenced by common factors. To describe such a phenomenon in degradations, a multi-degradation model with public noise is proposed. To identify the degradation states and the unknown parameters, an iterative estimation method is proposed by using the Kalman filter and the expectation maximization(EM) algorithm. Next, with known thresholds,the RUL of each degradation can be predicted by using the first hitting time(FHT). In addition, the RUL of the whole system can be obtained by a Copula function. Finally, a practical case is used to demonstrate the method proposed. | ZHANG Hanwen CHEN Maoyin ZHOU Donghua | 2018 | Journal of Systems Engineering and Electronics2018，29，2： | 6 |
| 6 | Mapping the immune microenvironment for mandibular alveolar bone homeostasis at single-cell resolution显示文摘Alveolar bone is the thickened ridge of jaw bone that supports teeth.It is subject to constant occlusal force and pathogens invasion,and is therefore under active bone remodeling and immunomodulation.Alveolar bone holds a distinct niche from long bone considering their different developmental origin and postnatal remodeling pattern.However,a systematic explanation of alveolar bone at single-cell level is still lacking.Here,we construct a single-cell atlas of mouse mandibular alveolar bone through single-cell RNA sequencing(scRNA-seq).A more active immune microenvironment is identified in alveolar bone,with a higher proportion of mature immune cells than in long bone.Among all immune cell populations,the monocyte/macrophage subpopulation most actively interacts with mesenchymal stem cells(MSCs)subpopulation.Alveolar bone monocytes/macrophages express a higher level of Oncostatin M(Osm)compared to long bone,which promotes osteogenic differentiation and inhibits adipogenic differentiation of MSCs.In summary,our study reveals a unique immune microenvironment of alveolar bone,which may provide a more precise immune-modulatory target for therapeutic treatment of oral diseases. | Weimin Lin Qiwen Li Danting Zhang Xiaohan Zhang Xingying Qi Qian Wang Yaqian Chen Caojie Liu Hanwen Li Shiwen Zhang Yuan Wang Bin Shao Li Zhang Quan Yuan | 2021 | Bone Research2021，9，1： | 6 |
| 7 | Ticagrelor inhibits the NLRP3 inflammasome to protect against inflammatory disease independent of the P2Y_(12 ) signaling pathway显示文摘Ticagrelor is the first reversibly binding oralP2Y_(12) receptor antagonist to inhibit platelet activation and has been approved by the Food and Drug Administration for the treatment of coronary artery disease. At present, the other pharmacological functions of ticagrelor remain poorly understood. The NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome plays a critical role in the innate immune system, but its excessive activation also contributes to the pathogenesis of complex diseases. In this study, we systematically examined the effects of ticagrelor on the NLRP3 inflammasome and found that ticagrelor inhibits NLRP3 inflammasome activation in macrophages independent of its classic inhibitory effect on theP2Y_(12) signaling pathway. Further mechanistic studies demonstrate that ticagrelor attenuates the oligomerization of apoptosis-associated speck-like protein containing a CARD (ASC) by blocking chloride efflux, an effect achieved through the degradation of chloride intracellular channel proteins (CLICs) and blockade of the translocation of CLICs to the plasma membrane. Moreover, experiments on lipopolysaccharide-induced sepsis and alum-induced peritonitis in mice confirmed that ticagrelor mitigates the severity of systemic inflammation independent ofP2Y_(12) receptor antagonism. Importantly, oral administration of ticagrelor rapidly and strongly inhibited NLRP3 inflammasome activation in peripheral blood mononuclear cells from patients with acute coronary syndrome. Overall, our study reveals a novel pharmacological function of ticagrelor in addition to its classic antiplatelet properties, which suggests that ticagrelor may serve as a potential therapeutic agent for use in NLRP3-associated diseases. | Bo Huang Yufeng Qian Shujun Xie Xianhua Ye Hanwen Chen Zhifeng Chen Lihuan Zhang Jinming Xu Hu Hu Shenglin Ma Paul Héroux Di Wang Han-Ming Shen Yihua Wu Dajing Xia | 2021 | Cellular & Molecular Immunology2021，18，5： | 3 |
| 8 | Self-contained eigenvector algorithm applied to the identification of aerodynamic derivatives of bridge model显示文摘On one hand, when the bridge stays in a windy environment, the aerodynamic power would reduce it to act as a non-classic system. Consequently, the transposition of the system’s right eigenmatrix will not equal its left eigenmatrix any longer. On the other hand, eigenmatrix plays an important role in model identification, which is the basis of the identification of aerodynamic derivatives. In this study, we follow Scanlan’s simple bridge model and utilize the information provided by the left and right eigenmatrixes to structure a self-contained eigenvector algorithm in the frequency domain. For the purpose of fitting more accurate transfer function, the study adopts the combined sine-wave stimulation method in the numerical simulation. And from the simulation results, we can conclude that the derivatives identified by the self-contained eigenvector algorithm are more dependable. | ZHANG XiaoXu CHEN LiFen SONG HanWen | 2011 | Science China(Technological Sciences)2011，54，5： | 3 |
| 9 | circStrn3 is involved in bone cancer pain regulation in a rat model显示文摘Bone cancer pain(BCP)is a common chronic pain that is caused by a primary or metastatic bone tumor,More detailed molecular mechanisms of BCP are warranted.In this study,we established a BCP rat model.The von Frey hair test,body weight,and hematoxylin and eosin staining were employed.We screened differentilly expressed circRNAs(DECs)between the BCP group and sham group.The results revealed that 850 DECs were significantly up-regulated and 644 DECs were significantly down-regulated in the BCP group.Furthermore,we identified 1177 diffrentially expressed genes(DEGs)significantly up-regulated and 565 DEGs significantly down-regulated in the BCP group.Gene Ontology annotation of all 1742 DEGs revealed that biological regulation of metabolic processes,cellular processes,and binding were the top enriched terrms.For Kyoto Ency-clopedia of Genes and Genomes analysis,phagosome,HTLV-I infection,proteoglycans in cancer,and herpes simplex infection were significantly enriched in this study.In addition,we identified four selected circRNAs,chr6:72418120|72430205.chr20:756105717573740,chr18:69943105|69944476,and chr5:167516581|167558250,by quantitative real time PCR.chr6:72418120|72430205(circStrn3)was selected for further study based on expression level and the circRNA--miRNA--mRNA network table.Western blot analysis suggested that knockdown of circStrn3 could effectively induce Walker 256 cell apoptosis.In summary,our study provided a more in-depth understanding of the molecular mechanisms of BCP. | Yiwen Zhang Xiaoxia Zhang Zumin Xing Shuyi Tang Hanwen Chen Zhongqi Zhang Jiyuan Li Yalan Li | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 2 |
| 10 | Association between borrower and lender state ownership and accounting conservatism显示文摘 | Chen Hanwen Chen J Z Lobo G J | 2010 | Journal of Accounting Research2010，48，5： | 1 |
| 11 | Dimensional Gradient Structure of CoSe2@CNTs-MXene Anode Assisted by Ether for High-Capacity,Stable Sodium Storage显示文摘Recently,abundant resources,low-cost sodium-ion batteries are deemed to the new-generation battery in the field of largescale energy storage.Nevertheless,poor active reaction dynamics,dissolution of intermediates and electrolyte matching problems are significant challenges that need to be solved.Herein,dimensional gradient structure of sheet-tube-dots is constructed with CoSe2@CNTs-MXene.Gradient structure is conducive to fast migration of electrons and ions with the association of ether electrolyte.For half-cell,CoSe2@CNTs-MXene exhibits high initial coulomb efficiency(81.7%)and excellent cycling performance(400 mAh g^-1 cycling for 200 times in 2 Ag^−1).Phase transformation pathway from crystalline CoSe2-Na2Se with Co and then amorphous CoSe2 in the discharge/charge process is also explored by in situ X-ray diffraction.Density functional theory study discloses the CoSe2@CNTs-MXene in ether electrolyte system which contributes to stable sodium storage performance owing to the strong adsorption force from hierarchical structure and weak interaction between electrolyte and electrode interface.For full cell,CoSe2@CNTs-MXene//Na3V2(PO4)3/C full battery can also afford a competitively reversible capacity of 280 mAh g^−1 over 50 cycles.Concisely,profiting from dimensional gradient structure and matched electrolyte of CoSe2@CNTs-MXene hold great application potential for stable sodium storage. | Enze Xu Pengcheng Li Junjie Quan Hanwen Zhu Li Wang Yajing Chang Zhenjie Sun Lei Chen Dabin Yu Yang Jiang | 2021 | Nano-Micro Letters2021，13，2： | 1 |
| 12 | Fucoidan antagonizes diet-induced obesity and inflammation in mice显示文摘Obesity is an escalating global pandemic posing a serious threat to human health.The intervention therapy using weight-reducing drugs,accompanied by lifestyle modification,is a strategy for the treatment of obesity.In the present study,we explored the role of fucoidan,a seaweed compound,on high-fat diet(HFD)-induced obesity in mice.We found that fucoidan treatment significantly reduced the body fat and caused redistribution of visceral and subcutaneous fat in HFD-fed mice.Meanwhile,fucoidan treatment inhibited adipocyte hypertrophy and inflammation in adipose tissue.Collectively,these results suggest that fucoidan may be a promising treatment for obesity and obesity-induced complications. | Lei Wang Kun Zhang Xin Ding Yan Wang Hui Bai Qing Yang Jingjing Ben Hanwen Zhang Xiaoyu Li Qi Chen Xudong Zhu | 2021 | The Journal of Biomedical Research2021，35，3： | 1 |
| 13 | Stochastic process-based degradation modeling and RUL prediction:from Brownian motion to fractional Brownian motion显示文摘Brownian motion(BM)has been widely used for degradation modeling and remaining useful life(RUL)prediction,but it is essentially Markovian.This implies that the future state in a BM-based degradation process relies only on its current state,independent of the past states.However,some practical industrial devices such as Li-ion batteries,ball bearings,turbofans,and blast furnace walls show degradations with long-range dependence(LRD),where the future degradation states depend on both the current and past degradation states.This type of degradation naturally brings two interesting problems,that is,how to model the degradations and how to predict their RULs.Recently,in contrast to the work that uses only BM,fractional Brownian motion(FBM)is introduced to model practical degradations.The most important feature of the FBM-based degradation models is the ability to characterize the non-Markovian degradations with LRD.Although FBM is an extension of BM,it is neither a Markovian process nor a semimartingale.Therefore,how to obtain the first passage time of an FBM-based degradation process has become a challenging task.In this paper,a review of the transition of RUL prediction from BM to FBM is provided.The peculiarities of FBM when addressing the LRD inherent in some practical degradations are discussed.We first review the BM-based degradation models of the past few decades and then give details regarding the evolution of FBM-based research.Interestingly,the existing BM-based models scarcely consider the effect of LRD on the prediction of RULs.Two practical cases illustrate that the newly developed FBMbased models are more generalized and suitable for predicting RULs than the BM-based models,especially for degradations with LRD.Along with the direction of FBM-based RUL prediction,we also introduce some important and interesting problems that require further study. | Hanwen ZHANG Maoyin CHEN Jun SHANG Chunjie YANG Youxian SUN | 2021 | Science China(Information Sciences)2021，64，7： | 1 |
| 14 | Research Eval- uation of Research-oriented Universities in Taiwan from 1993 to 2003显示文摘 | Huang Muhsuan Chang Hanwen Chen Darzen | 2006 | Scientometrics2006，，67： | 1 |
| 15 | In vivo inducing collagen regeneration of biodegradable polymer microspheres显示文摘Biodegradable polymer particles have been used as dermal fillers for pre-clinical and clinical trials.The impact of material properties of polymers is very important to develop products for aesthetic medicine such as dermal fillers.Herein,eight biodegradable polymers with different molecular weights,chemical compositions or hydrophilic-hydrophobic properties were prepared and characterized for systematical study for aesthetic medicine applications.Polymer microspheres with 20-100 lmwere prepared.The in vitro degradation study showed that poly(L-lactic-co-glycolic acid)75/25 microspheres degraded the fastest,whereas poly(L-lactic acid)(PLLA)microspheres with intrinsic viscosity of 6.89([g]¼6.89)with the highest molecular weight showed the slowest degradation rate.After these microspheres were fabricated dermal fillers according to the formula of SculptraVR,they were injected subcutaneously into the back skin of rabbits.In vivo results demonstrated that the degradation rate of microspheres strongly correlated with the foreign body reaction and collagen regeneration was induced by microspheres.The microspheres with faster degradation rate induced inflammatory response and the collagen regeneration maintained in shorter time.PLLA([g]¼3.80)microsphere with a moderate molecular weight and degradation rate could strongly regenerate Type I and III collagen to maintain a long-term aesthetic medicine effect.These properties of size,morphology and degradation behavior would influence the foreign body reaction and collagen regeneration. | Yixin Zhang Hanwen Liang Qian Luo Jianlin Chen Nan Zhao Wenxia Gao Yuji Pu Bin He Jing Xie | 2021 | Regenerative Biomaterials2021，8，5： | 1 |
| 16 | Effects of Audit Quality on Earnings Management and Cost of Equity Capital:Evidence from China显示文摘 | Hanwen Chen Jeff Z Chen Gerald Lobo Yanyan Wang | 2011 | Contemporary Accounting Research2011，，： | 1 |
| 17 | Sensitive determination of diethylstilbestrol by high performance liquid chromatography with fluorescence detection with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a labeling reagent显示文摘 | CHEN Peiyun SUN Hanwen WANG Xianyou | 2012 | Analytical Methods2012，4，12： | 1 |
| 18 | Regulation of nitrogen starvation responses by the alarmone(p)ppGpp in rice显示文摘Nitrogen is an essential macronutrient for all living organisms and is critical for crop productivity and quality.In higher plants,inorganic nitrogen is absorbed through roots and then assimilated into amino acids by the highly conserved glutamine synthetase/glutamine:2-oxoglutarate aminotransferase(GS/GOGAT)cycle.How nitrogen metabolism and nitrogen starvation responses of plants are regulated remains largely unknown.Previous studies revealed that mutations in the rice ABNORMAL CYTOKININ RESPONSE1(ABC1)gene encoding Fd-GOGAT cause a typical nitrogen deficiency syndrome.Here,we show that ARE2(for ABC1 REPRESSOR2)is a key regulator of nitrogen starvation responses in rice.The are2 mutations partially rescue the nitrogen-deficient phenotype of abc1 and the are2 mutants show enhanced tolerance to nitrogen deficiency,suggesting that ARE2 genetically interacts with ABC1/Fd-GOGAT.ARE2 encodes a chloroplast-localized Rel A/Spo T homolog protein that catalyzes the hydrolysis of guanosine pentaphosphate or tetraphosphate(p)pp Gpp,an alarmone regulating the stringent response in bacteria under nutritional stress conditions.The are2 mutants accumulate excessive amounts of(p)pp Gpp,which correlate with lower levels of photosynthetic proteins and higher amino acid levels.Collectively,these observations suggest that the alarmone(p)pp Gpp mediates nitrogen stress responses and may constitute a highly conserved mechanism from bacteria to plants. | Hanwen Li Jinqiang Nian Shuang Fang Meng Guo Xiahe Huang Fengxia Zhang Qing Wang Jian Zhang Jiaoteng Bai Guojun Dong Peiyong Xin Xianzhi Xie Fan Chen Guodong Wang Yingchun Wang Qian Qian Jianru Zuo Jinfang Chu Xiaohui Ma | 2022 | Journal of Genetics and Genomics2022，49，5： | 1 |
| 19 | Wide-beam SAR autofocus based on blind resampling显示文摘Long synthetic aperture time and large instantaneous beam can turn airborne synthetic aperture radar(SAR)autofocus into a wide-beam autofocus problem;i.e.,the motion error is both range-and azimuth-spatial variant.A typical two-step Mo Co method cannot process wide-beam airborne SAR data.Moreover,traditional wide-beam SAR Mo Co algorithms,such as sub-aperture topography and aperturedependent(SATA),precise topography-and aperture-dependent(PTA),and frequency division(FD),are highly dependent on high-precision inertial navigation system/global positioning system(INS/GPS)data and belong to the sub-aperture method,which may result in serious grant-lobe or stitching problems in the image.Alternatively,this article proposes a full-aperture autofocus method for wide-beam SAR based on blind RS.The proposed method does not require INS/GPS data and avoids the problems of traditional subaperture methods,which can significantly improve the overall image quality.The measured data processing results of the wide-beam SAR verify the effectiveness of the proposed algorithm. | Jianlai CHEN Hanwen YU | 2023 | Science China(Information Sciences)2023，66，4： | 1 |
| 20 | A frequency-domain method for solving linear time delay systems with constant coefficients显示文摘In an active control system, time delay will occur due to processes such as signal acquisition and transmission, calculation,and actuation. Time delay systems are usually described by delay differential equations(DDEs). Since it is hard to obtain an analytical solution to a DDE, numerical solution is of necessity. This paper presents a frequency-domain method that uses a truncated transfer function to solve a class of DDEs. The theoretical transfer function is the sum of infinite items expressed in terms of poles and residues. The basic idea is to select the dominant poles and residues to truncate the transfer function,thus ensuring the validity of the solution while improving the efficiency of calculation. Meanwhile, the guideline of selecting these poles and residues is provided. Numerical simulations of both stable and unstable delayed systems are given to verify the proposed method, and the results are presented and analysed in detail. | Mengshi Jin Wei Chen Hanwen Song Jian Xu | 2018 | Acta Mechanica Sinica2018，34，4： | 1 |
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