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| 1 | DFT study of new bipyrazole derivatives and their potential activity as corrosion inhibitors显示文摘 | Hengliang Wang Xueye Wang Hanlu Wang Ling Wang Aihong Liu | 2007 | J Mol Model2007，13，： | 1 |
| 2 | Friction and wear behavior of three-dimensional braided carbon fiber/epoxy composites under lubricated sliding conditions显示文摘 | Wan Yizhi Luo Hengliang Wang Yongluo | 2005 | J Mater Sci2005，40，17： | 1 |
| 3 | A homologous and molecular dual-targeted biomimetic nanocarrier for EGFR-related non-small cell lung cancer therapy显示文摘The abnormal activation of epidermal growth factor receptor(EGFR)drives the development of non-small cell lung cancer(NSCLC).The EGFR-targeting tyrosine kinase inhibitor osimertinib is frequently used to clinically treat NSCLC and exhibits marked efficacy in patients with NSCLC who have an EGFR mutation.However,free osimertinib administration exhibits an inadequate response in vivo,with only~3%patients demonstrating a complete clinical response.Consequently,we designed a biomimetic nanoparticle(CMNP^(@Osi))comprising a polymeric nanoparticle core and tumor cell-derived membrane-coated shell that combines membrane-mediated homologous and molecular targeting for targeted drug delivery,thereby supporting a dual-target strategy for enhancing osimertinib efficacy.After intravenous injection,CMNP^(@Osi)accumulates at tumor sites and displays enhanced uptake into cancer cells based on homologous targeting.Osimertinib is subsequently released into the cytoplasm,where it suppresses the phosphorylation of upstream EGFR and the downstream AKT signaling pathway and inhibits the proliferation of NSCLC cells.Thus,this dual-targeting strategy using a biomimetic nanocarrier can enhance molecular-targeted drug delivery and improve clinical efficacy. | Bin Xu Fanjun Zeng Jialong Deng Lintong Yao Shengbo Liu Hengliang Hou Yucheng Huang Hongyuan Zhu Shaowei Wu Qiaxuan Li Weijie Zhan Hongrui Qiu Huili Wang Yundong Li Xianzhu Yang Ziyang Cao Yu Zhang Haiyu Zhou | 2023 | Bioactive Materials2023，，9： | 1 |
| 4 | Orthogonal modular biosynthesis of nanoscale conjugate vaccines for vaccination against infection显示文摘Conjugate vaccines represent one of the most effective means for controlling the occurrence of bacterial diseases.Although nanotechnology has been greatly applied in the field of vaccines,it is seldom used for conjugate vaccine research because it is very difficult to connect polysaccharides and nanocarriers.In this work,an orthogonal and modular biosynthesis method was used to produce nanoconjugate vaccines using the SpyTag/SpyCatcher system.When SpyTag/SpyCatcher system is combined with protein glycosylation technology,bacterial O-polysaccharide obtained from Shigela flexneri 2a can be conjugated onto the surfaces of different virus-like particles(VLPs)in a biocompatible and controlled manner.After confirming the excellent lymph node targeting and humoral immune activation abilities,these nanoconjugate vaccines further induced efficient prophylactic effects against infection in a mouse model.These results demonstrated that natural polysaccharide antigens can be easily connected to VLPs to prepare highly efficient nanoconjugate vaccines.To the best of the researchers1 knowledge,this is the first time VLP-based nanoconjugate vaccines are produced efficiently,and this strategy could be applied to develop various pathogenic nanoconjugate vaccines. | Xin Li Chao Pan Peng Sun Zhehui Peng Erling Feng Jun Wu Hengliang Wang Li Zhu | 2022 | Nano Research2022，15，2： | 1 |
| 5 | Immunoproteomics of outer membrane proteins and extracellular proteins of Shigella flexneri 2a 2457T 显示文摘 | Ying Tianyi Wang Hengliang Li Mingzhu | 2005 | Proteomics2005，5，： | 1 |
| 6 | Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells显示文摘An in vivo expression technology (IVET) was applied to screen s.flexneri 2a genes induced after invasion of epithelial cells, and virulence-related genes were further identified by mutational analysis. Thirteen intracellular induced genes were identified with a HeLa cell infection model. Of these, two were identified as alkylation-related genes; one was related to metabolism; one encoded a transcriptional regulator; three were identified as insertion elements; three ap- peared to be antisense to genes involved in the transmethylation,biosyntheseis, and phos- photransferase system;and three were predicted to encode polypeptides with unknown functions. Intracellular survival assavs showed that the mutants of alkA,citC and wcaJ genes had lower capability of intracellular replication or survival than the the wild-type strain.The results indicated that alkA, citC and wcaJ genes could take part in the intracellular survival or replication of S. flexneri 2a and the capability of intracellular survival or replication could be one of the major virulence elements. However, the yaiC mutant was able to survive in the murine infection assay but almost not in HeLa cell infection assay. Very possibly, yaiC gene was involved in the other mechanism of S. flexneri virulence. This study might lead to a better understanding of the intra- cellular survival or proliferation process of S. flexneri 2a and perhaps provide insights into the pathogenicity of this pathogen. | SHI Zhaoxing WANG Hengliang HU Kun FENG Erling YAO Xiao HUANG Liuyu SU Guofu HUNAG Peitang HUANG Cuifen | 2004 | Science China(Life Sciences)2004，47，6： | 0 |
| 7 | Media Effect of Students’ Ideological and Political Work in Tibetan Agricultural Colleges and Universities显示文摘Both the students’ ideological and political work and the media work in agricultural colleges and universities are priorities of agricultural higher education in Tibet. The economic and social development situation in Tibet,especially the modern agricultural development trend in Tibet,provides excellent development opportunities and challenges for both the students’ ideological and political work and the media work in Tibetan agricultural colleges and universities. On the whole,the students’ ideological and political work and the media work should not be treated with discrimination. Instead,they should be effectively integrated and coordinated,to form joint efforts,so as to create a bright spring for agricultural higher education of Tibet. | Hengliang WANG | 2017 | Asian Agricultural Research2017，9，6： | 0 |
| 8 | 空肠间置术能快速改善非肥胖2型糖尿病大鼠的糖耐量并缓慢改善胰岛素抵抗显示文摘背景:许多研究已证实,回肠间置术能改善2型糖尿病(T2DM),同时胰高血糖素样肽-1(GLP-1)水平上升。我们采用糖尿病大鼠模型进行研究,评估回肠间置术对糖代谢的影响及其机制。方法:20只GK大鼠被随机分为回肠间置组和假手术组。分别于术前及术后1、4、8、16、24周检测大鼠体重、进食量、空腹血糖(FPG)、空腹胰岛素(F-ins)、口服葡萄糖耐量试验(OGTT)和GLP-1水平。计算稳态模型胰岛素抵抗指数(HOMA-IR)和OGTT曲线下面积(AUC)。术后24周处死大鼠,检测胰腺和回肠组织中GLP-1受体(GLP-1R)的表达水平。结果:与假手术组相比,回肠间置组大鼠术后4、8、16和24周血GLP-1水平更高(均P<0.05),术后8、16和24周FPG、F-ins和HOMAIR水平更低(均P<0.05)。与术前相比,回肠间置组术后各个时间点均可观察到血GLP-1、AUC和FPG水平的下降(均P<0.05);术后8、16和24周F-ins和HOMA-IR也显著下降(均P<0.05)。术后24周,无论是胰腺还是回肠组织,回肠间置组大鼠的GLP-1R表达水平均显著高于假手术组(均P<0.05)。结论:回肠间置可改善GK大鼠的糖代谢,且不会导致体重的下降和进食量的减少,这可能与GLP-1水平升高有关。而后期胰岛素抵抗的改善和胰岛素水平的下降可能并不直接受益于GLP-1水平的升高。 | Hengliang Zhu Huaiming Wang Zhihai Zheng Bailiang Ye Xiaojiao Ruan Xiaofeng Zheng Guoxin Li | 2018 | Gastroenterology Report2018，6，4： | 0 |
| 9 | A novel aberrant splicing of G_(sa) transcript in human leukemia cell lines显示文摘The α subunit of the stimulatory G protein, Gsα, is involved in the stimulation of adenylate cyclase pathway of signal transduction. The various Gsα mRNAs are generated either by alternative splicing or by using alternative promoter. A novel aberrant splicing of Gsα transcript in human leukemia cell lines is reported. The entire coding region of Gsα gene obtained, referred to as GsαLeu, was amplified by PCR with cDNA from Jurkat human leukemia cDNA library or the reverse-transcribed first strand cDNA from human leukemia cell line HL-60 as a template. The result of DNA sequencing indicated that,compared with originally reported Gsα, GsαLeu has two in-frame deletions, one in amino acid residues 23—249 and the other in amino acid residues 254—260, suggesting that G(sαLeu) may play an important role in leukemia. The complete coding region of GsαLu was inserted downstream of the thrombin site of pGEX-2T fusion protein expression vector and expressed in the form of GST/G( | YE Qinong, YAO Xiao, WANG Hengliang, ZHANG Shu, LIU Huaitian, SU Guofu, HUANG Cuifen and ZHOU Tingchong Beijing Institute of Biotechnology, Beijing 100071, China Department of Biology, Shaanxi Normal College, Xi’an 710062, China Beijing Medical University, School of Oncology, Beijing 100036, China Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China Beijing Institute of Basic Medicine Sciences , Beijing 100850, China | 1999 | Chinese Science Bulletin1999，44，4： | 0 |
| 10 | Bautype: Capsule and Lipopolysaccharide Serotype Prediction for Acinetobacter baumannii Genome显示文摘Acinetobacter baumannii has three clusters of genes for polysaccharide synthesis.Capsular polysaccharides and lipopolysaccharides are major virulence determinants in many Gram-negative bacteria,and play key roles in the overall fitness and virulence in A.baumannii,meanwhile poly-beta-1-6-N-acetylglucosamine was critical for biofilm formation.A variety of studies have been conducted which focused on the surveillance of antimicrobial resistance genes and clones in A.baumannii.However,a tool to perform serotype prediction in A.baumannii is still lacking.Here we present Bautype,a tool for whole genome sequencingbased K and O locus typing in A.baumannii.Using Bautype,users can upload and analyze the genome assembled in a web browser,with the results being displayed in a tabular or graphical format.We demonstrated the high accuracy of predicting K and O locus diversity using Bautype when analyzing more than 3000 A.baumannii genomes.Using our software,we were able to analyze K and O locus diversity among 593 genomes belonging to ST208(3 K and 8 O).These diverse loci can serve as epidemiological markers for Multi-drug Resistance(MDR)strain subtyping.We also identified 45 novel O locus types.Our findings highlight the importance in monitoring the capsule type and LOS of A.baumannii strains for which Bautype is a tailored clinical software.Bautype is available at http://BauType.net. | Xiaoting Hua Qian Liang Li Fang Jintao He Meixia Wang Wenjie Hong Sebastian Leptihn Hengliang Wang Yunsong Yu Huan Chen | 2020 | Infectious Microbes & Diseases2020，2，1： | 0 |
| 11 | Preliminary study on discovery of a novel molecule that interacts with CPP32 using two-hybrid system显示文摘Of ICE protease family, CPP32 (apopain or Yama) plays a central role in different apoptotic pathways. To study the molecules regulating CPP32, yeast two_hybrid system was used to identify proteins (peptides) that interact with CPP32. First, the CPP32 gene was cloned into plasmid vector pGBT9. The resulting recombinant plasmid was designated as pGBT9/CPP. The pGBT9 /CPP plasmid was transformed into the yeast strain HF7C, then the leukemia library was introduced. The transformation mixture was plated on medium lacking Trp, Leu and His in the initial screen. Colonies growing on the selection medium were further assayed for β galactosidase activity. Within 42 Trp +Leu +His + colonies only 5 turned blue in the presence of X_Gal. Plasmid DNA from 5 positive yeast colonies was prepared respectively and used to transform HB101 by electroporation. The transformation mixture was plated on medium lacking Leu to be selected for the library plasmid. Finally, only one library plasmid, designated as pY1, was determined to be truly positive by retransformation of pGBT9/CPP and the library plasmid into HF7C. The inserted cDNA of pY1 encodes a peptide of 15 amino acids, suggesting that it may be the domain interacting with CPP32. | Ye Qinong Yao Xiao Wang Hengliang Su Guofu Huang Cuifen Zhou Tingchong | 1998 | Chinese Science Bulletin1998，43，4： | 0 |
| 12 | Recent Advance of Tellurium for Biomedical Applications显示文摘Tellurium(Te)nanomaterials have aroused a wide interest in semiconductor,thermoelectric,piezoelectric,as well as biomedical applications.The last decades of reports indicated a major nanostructure of one-dimensional(ID)Te on account of its inherent structural anisotropy.Two-dimenional(2D)Te has been newly developed and drawn a lot of interests recently.This review presents the intrinsic biological potential of Te-based nanomaterials and summarizes their up-to-date advance in phototherapy and reactive oxygen species(ROS)-related applications in the biomedical field. | WANG Hengliang CHAI Luxiao XIE Zhongjian ZHANG Han | 2020 | Chemical Research in Chinese Universities2020，36，4： | 0 |
| 13 | Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine Against Brucella melitensis显示文摘Brucellosis,caused by Brucella,is one of the most common zoonosis.However,there is still no vaccine for human use.Although some live attenuated vaccines have been approved for animals,the protection effect is not ideal.In this study,we developed a dual-antigen nanoconjugate vaccine containing both polysaccharide and protein antigens against Brucella.First,the antigenic polysaccharide was covalently coupled to the outer membrane protein Omp19 using protein glycan coupling technology,and then it was successfully loaded on a nano-carrier through the SpyTag/SpyCatcher system.After confirming the efficient immune activation and safety performance of the dual-antigen nanoconjugate vaccine,the potent serum antibody response against the two antigens and remarkable protective effect in non-lethal and lethal Brucella infection models were further demonstrated through different routes of administration.These results indicated that the dual-antigen nanoconjugate vaccine enhanced both T helper 1 cell(Th1)and Th2 immune responses and protected mice from Brucella infection.Furthermore,we found that this protective effect was maintained for at least 18 weeks.To our knowledge,this is the first Brucella vaccine bearing diverse antigens,including a protein and polysaccharide,on a single nanoparticle.Thus,we also present an attractive technology for co-delivery of different types of antigens using a strategy applicable to other vaccines against infectious diseases. | Jing Huang Yufei Wang Kangfeng Wang Shulei Li Peng Sun Yan Guo Jiankai Liu Ruifu Yang Ming Zeng Chao Pan Hengliang Wang Li Zhu | 2023 | Engineering2023，，10： | 0 |
| 14 | Construction of Entrepreneurship Courses in Tibetan Agriculture and Forestry Colleges and Universities显示文摘Both the state and the Tibet Autonomous Region always care about and attach great importance to the construction of entrepreneurship courses and enterprise education of college students.In the context of mass entrepreneurship and innovation,the entrepreneurship courses of Tibetan agriculture and forestry colleges and universities undertake the unique historical mission.As the important guarantee for cultivating entrepreneurship and innovative talents of Tibetan agriculture and forestry disciplines,the entrepreneurship courses of Tibetan agriculture and forestry colleges and universities have unique and important theoretical and realistic significance.Through the overall survey and analysis of the construction of entrepreneurship courses in Tibetan agriculture and forestry colleges and universities,this paper made clear problems and obstacles in the construction of entrepreneurship courses in Tibetan agriculture and forestry colleges and universities,and came up with scientific,reasonable,and feasible recommendations,to provide support for long-term mechanism construction of entrepreneurship courses in Tibetan agriculture and forestry colleges and universities and even other areas. | Hengliang WANG | 2017 | Asian Agricultural Research2017，9，8： | 0 |
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