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| 1 | High-level expression and secondary structure analysis of the bovine mature prion protein显示文摘By using the recombinant DNA technology, the gene of the bovine mature prion protein bPrPcL) has been cloned into pET30a and the resulting plasmid has been expressed in E.coli BL21(DE3). After solubilizing in 8 mol/L urea, the expression product was purified by cation ion exchange chromatography. The purified product was refolded by dilution and the recovery was about 15%. Analysis of mass spectrum, circular dichroism (CD) spectrum and Fourier transform infrared (FTIR) spectrum demonstrate that the molecular weight of the bPrPcL is 23 630 u, the bPrPcL has a high α-helix content (36.1%) and low (3-sheet content (11.9%). | WANG Dawei YANG Huaiyi RAO Zihe TIAN Bo | 2000 | Chinese Science Bulletin2000，45，14： | 3 |
| 2 | Extremely low penetrance of deafness associated with the mitochondrial 12S rRNA mutation in 16 Chinese families: Implication for early detection and prevention of deafness显示文摘 | Pu Dai Xin Liu Dongyi Han Yaping Qian Deliang Huang Huijun Yuan Weiming Li Fei Yu Ruining Zhang Hongyan Lin Yong He Youjun Yu Quanzhu Sun Huaiyi Qin Ronghua Li Xin Zhang Dongyang Kang Juyang Cao Wie-Yen Young Min-Xin Guan | 2005 | Biochemical and Biophysical Research Communications2005，，1： | 2 |
| 3 | Development of multi-view video coding using hierarchical B picture显示文摘 | Huaiyi Pan | | 0，，01： | 1 |
| 4 | Real- time tracking mitochondrial dynamic remodeling with two-photon phosphorescent iridium (Ⅲ) complexes 显示文摘 | Huang Huaiyi Yang Liang Zhang Pingyu | 2016 | Biomaterials2016，83，： | 1 |
| 5 | Perturbing tumor cell metabolism with a Ru(Ⅱ)photo-redox catalyst to reverse the multidrug resistance of lung cancer显示文摘The novel concept of enzyme photocatalysis has recently attracted much attention in developing anticancer therapy.However,the relationship between coenzyme depletion and cellular metabolomic changes has rarely been investigated.Herein,we report the rational design of a deep-red light-triggered bis-tridentate Ru(Ⅱ)photocatalyst(Ru3),which induces cell metabolism disorder to combat multidrug resistance.Ru3 exhibits promising multiple triplet excited states,a long lifetime,and high photocatalytic activity toward the coenzyme.Consequently,Ru3 shows high phototherapeutic activity(photo index=191–833)against diverse resistant(cisplatin,5-fluorouracil,or paclitaxel)lung cancer cells by inhibiting cellular peptide,lipid,and glycerophospholipid metabolism.We believe that cell metabolism inhibition by photo-redox catalysts is an effective form of therapeutics for drug-resistant cancer cells. | Siqi Wei Hui Liang Anyi Dao Yuzhen Xie Fengshu Cao Qingyan Ren Ashish Kumar Yadav Rajesh Kushwaha Arif Ali Mandal Samya Banerjee Pingyu Zhang Shaomin Ji Huaiyi Huang | 2023 | Science China Chemistry2023，66，5： | 1 |
| 6 | Ribozyme-mediated resistance to rice dwarf virus and the transgene silencing in the progeny of transgenic rice plants显示文摘 | Shengcheng Han Zujian Wu Huaiyi Yang Rong Wang Yin Yie Lianhui Xie Po Tien | 2000 | Transgenic Research2000，，3： | 1 |
| 7 | Area-Efficient VLSI Design of Reed Solomon Decoder for 10GBase-LX4 Optical Communication Systems显示文摘 | Huaiyi Hsu Anyeu Wu Jihchiang Yeo | 2006 | IEEE Trans on circuits and systems2006，53，11： | 1 |
| 8 | Cathepsin K contributed to disturbed flow-induced atherosclerosis is dependent on integrin-actin cytoskeleton–NF–κB pathway显示文摘Atherosclerosis is a chronic inflammatory disease, occurring preferentially in bifurcation, branching, and bending of blood vessels exposed to disturbed flow. Disturbed flow in atheroprone areas activates elevated proteases, degrading elastin lamellae and collagenous matrix, resulting in endothelial dysfunction and vascular remodeling. As a mediator for extracellular matrix protein degradation, cathepsin K (CTSK) was directly regulated by hemodynamics and contributed to atherosclerosis. The mechanism of CTSK responding to disturbed flow and contributing to disturbed flow-induced atherosclerosis is unclear. In this study, the partial carotid ligation model of mice and in vitro disturbed shear stress model were constructed to explore the contribution and potential mechanism of CTSK in atherosclerosis. Our results indicated that CTSK elevated in the disturbed flow area in vivo and in vitro along with endothelial inflammation and atherogenesis. Additionally, the expression of integrin αvβ3 was upregulated in these atheroprone areas. We found that inhibition of the integrin αvβ3-cytoskeleton pathway could significantly block the activation of NF-κB and the expression of CTSK. Collectively, our findings unraveled that disturbed flow induces increased CTSK expression, and contributes to endothelial inflammation and vascular remodeling, leading to atherogenesis eventually. This study is helpful to provide new enlightenment for the therapy of atherosclerosis. | Fei Fang Tang Feng Jianwei Li Huaiyi Zhang Qin Wang Yidan Chen Guixue Wang Yang Shen Xiaoheng Liu | 2023 | Genes & Diseases2023，10，2： | 1 |
| 9 | Petrochemical plant process piping installation design manual显示文摘 | Zhang Dejiang Wang Huaiyi Liu Shaoye | 2005 | First Design and Calculation2005，，： | 1 |
| 10 | Breakthrough in construction of oxygen-independent photosensitizer for type Ⅲ photodynamic therapy显示文摘Cancer remains one of the leading causes of mortality worldwide.Despite enormous research efforts over the last several decades,death caused by cancer is predicted to rise by ca.70%within 2030 [1].Traditional treatment modalities involve removal of the primary tumor by surgery and further. | Zhishang Zhang Huaiyi Huang | 2022 | Science China Chemistry2022，65，5： | 0 |
| 11 | Photo-catalytic Staphylococcus aureus inactivation and biofilm destruction with novel bis-tridentate iridium(Ⅲ) photocatalyst显示文摘Bacterial infection is currently a serious challenge globally, causing death of thousands of human beings. New antimicrobial agents with novel mechanism of action are urgently needed. Transition metal complexes have shown great potentials in photodynamic and photocatalytic therapy. Herein, we take full advantage of metal photocatalyst and successfully developed a novel cyclometalated iridium(Ⅲ) complex(Ir1) with higher biofilm damage efficiency than the clinical antibiotics. Ir1 synergistically generates reactive oxygen species and coenzyme photocatalytic activity with high efficiency under white light irradiation. Combined with these properties, Ir1 exhibited excellent photoinactivation of S. aureus and effectively damaged the biofilm. This work provides a new approach for the development of antibacterial photodynamic therapy. | Shihao Chen Zhishang Zhang Li Wei Zhongxian Fan Yue Li Xuezhi Wang Tongming Feng Huaiyi Huang | 2023 | Chinese Chemical Letters2023，34，11： | 0 |
| 12 | Ultrasound-active ReCORM-AIEgen for gas and sonodynamic therapy of mycobacterium biofilms显示文摘Bacterial infection is the leading cause of many severe inflammation diseases. The development of novel and effective therapeutic approaches to counter bacterial infections, especially for drug-resistant bacteria, is essential. Herein, we have successfully developed an ultrasound-active tricarbonyl rhenium(Ⅰ) complex with tetraphenylethylene(TPE) modification(RePyTPE) for CO gas therapy and sonodynamic therapy of bacterial infections. RePy-TPE produced reactive oxygen species and released CO under ultrasound irradiation. In addition, RePy-TPE showed aggregation-induced emission in water due to the introduction of TPE, which enhanced the yield of ^(1)O_(2) generation in a biological aqueous solution. The produced ^(1)O_(2) and released CO killed mycobacterium smegmatis(M. smegmatis) and Escherichia coli(E. coli), as shown by bacterial membrane damage and biofilm elimination. Furthermore, ultrasound-active RePy-TPE perturbed the purine metabolism of the bacteria, which disturbed the biosynthesis of DNA and energy metabolism, eventually reducing the vitality of bacteria. This article provides a novel strategy for the development of ultrasound-active metal-based antibiotics. | Yue Li Haobing Wang Qitian Lin Xinyu Yu Huaiyi Huang Pingyu Zhang | 2023 | Science China Chemistry2023，66，9： | 0 |
| 13 | Highly luminescent zero-dimensional lead-free manganese halides forβ-ray scintillation显示文摘Because of their moderate penetration power,β-rays(high-energy electrons)are a useful signal for evaluating the surface contamination of nuclear radiation.However,the development ofβ-ray scintillators,which convert the absorbed high-energy electrons into visible photons,is hindered by the limitations of materials selection.Herein,we report two highly luminescent zerodimensional(0D)organic-inorganic lead-free metal halide hybrids,(C_(13)H_(30)N)_(2)MnBr_(4)and(C_(19)H_(34)N)_(2)MnBr_(4),as scintillators exhibiting efficientβ-ray scintillation.These hybrid scintillators combine the superior properties of organic and inorganic components.For example,organic components that contain light elements C,H,and N enhance the capturing efficiency ofβparticles;isolated inorganic[MnBr_(4)]2−tetrahedrons serve as highly localized emitting centers to emit intense radioluminescence(RL)underβ-ray excitation.Both hybrids show a narrow-band green emission peaked at 518 nm with photoluminescence quantum efficiencies(PLQEs)of 81.3%for(C_(13)H_(30)N)_(2)MnBr_(4)and 86.4%for(C_(19)H_(34)N)_(2)MnBr_(4),respectively.To enable the solution processing of this promising metal halide hybrid,we successfully synthesized(C_(13)H_(30)N)_(2)MnBr_(4)colloidal nanocrystals for the first time.Being excited byβ-rays,(C_(13)H_(30)N)_(2)MnBr_(4)scintillators show a linear response toβ-ray dose rate over a broad range from 400 to 2,800 Gy·s^(−1),and also display robust radiation resistance that 80%of the initial RL intensity can be maintained after an ultrahigh accumulated radiation dose of 240 kGy.This work will open up a new route for the development ofβ-ray scintillators. | Linyuan Lian Wei Qi Huaiyi Ding Hao Tian Qi Ye Yong-Biao Zhao Long Zhao Jianbo Gao Daoli Zhang Jianbing Zhang | 2022 | Nano Research2022，15，9： | 0 |
| 14 | Activity of the truncated promoter of pollen-specific G9 gene of cotton and the transgenic recessive nuclei-sterile tobacco显示文摘A 557 bp fragment from the translation initiation site of the G9 gene expressed in maturing pollens of cotton was isolated from genomic DNA of upland cotton (Gossypium hirsutum L.) cv. “Zhongkang 17”, and two expression vectors for plant transformation were constructed via fusing this fragment with β-glucuronidase gene (Gus) and cytotoxin gene Barnase. The promoter activity of this fragment was demonstrated via transient expression of Gus gene in cotton and by the integrated expression of Barnase gene in tobacco. This promoter can initiate the expression of exogenous gene specifically and efficiently in plant pollen. The transgenic tobacco plant containing G9-Barnase fusion gene showed the characteristics of recessive nuclei-sterility. | MA Hong, WANG Huanyu, LIU Yule, YANG Huaiyi and TIAN Bo(Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China) | 2002 | Progress in Natural Science:Materials International2002，12，3： | 0 |
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