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| 1 | The FTO/miR-181b-3p/ARL5B signaling pathway regulates cell migration and invasion in breast cancer显示文摘Background:N6-methyladenosine(m6A)RNA modification has been demonstrated to be a significant regulatory process in the progression of various tumors,including breast cancer.Fat mass and obesity-associated(FTO)enzyme,initially known as the obesity-related protein,is the first identified m6A demethylase.However,the relationship between FTO and breast cancer remains controversial.In this study,we aimed to elucidate the role and clinical significance of FTO in breast cancer and to explore the underlying mechanism.Methods:We first investigated the expression of FTO in breast cancer cell lines and tissues by quantitative reverse transcription-PCR(qRT-PCR),Western blotting,and immunohistochemistry.Wound healing assay and Transwell assay were performed to determine the migration and invasion abilities of SKBR3 and MDAMB453 cells with either knockdown or overexpression of FTO.RNA sequencing(RNA-seq)was conducted to decipher the downstream targets of FTO.qRT-PCR,luciferase reporter assay,and Western blotting were employed to confirm the existence of the FTO/miR-181b-3p/ARL5B axis.The biological function of ADP ribosylation factor like GTPase 5B(ARL5B)in breast cancer cells was evaluated by wound healing assay and Transwell invasion assay.Results:High FTO expression was observed in human epidermal growth factor receptor 2(HER2)-positive breast cancer,predicting advanced progression(tumor size[P<0.001],nuclear grade[P=0.001],peritumoral lymphovascular invasion[P<0.001),lymph node metastasis[P=0.002],and TNM stage[P=0.001])and poor prognosis.Moreover,FTO promoted cell invasion and migration in vitro.Mechanistically,RNA-seq and further confirmation studies suggested that FTO up-regulated ARL5B by inhibiting miR-181b-3p.We further verified that ARL5B also displayed carcinogenic activity in breast cancer cells.Conclusion:Our work demonstrated the carcinogenic activity of FTO in promoting the invasion and migration of breast cancer cells via the FTO/miR-181b-3p/ARL5B signaling pathway. | Yuanyuan Xu Shuang Ye Nan Zhang Shuhui Zheng Huatao Liu Kewen Zhou Ling Wang Yue Cao Peng Sun Tinghuai Wang | 2020 | Cancer Communications2020，40，10： | 24 |
| 2 | Biore mediation: A review of applications and problems to be resolved显示文摘This review article describes the factors affecting bioremediation processes including: goals of bioremediation and the optimal ecological conditions required; inoculation of microorganisms; co-metabolism; bioavailability and its improvement; biological evolution and its utilization; monitoring and control of bioremediation processes; identification of bioremediation effectiveness and ecological remediation and its key elements. The current progress in bioremediation techniques is summarized. The direction of future development, research and applications is also examined. | ZHOUOixing HUATao | 2004 | Progress in Natural Science:Materials International2004，14，11： | 11 |
| 3 | Inhibition of lipid oxidation in foods and feeds and hydroxyl radical-treated fish erythrocytes:A comparative study of Ginkgo biloba leaves extracts and synthetic antioxidants显示文摘This study explored the effects of butylated hydroxytoluene(BHT) and ethoxyquin(EQ) and ethyl ether extracts, ethyl acetate extracts(EAE), acetone extracts, ethanol extracts and aqueous extracts of Ginkgo biloba leaves(EGbs) on lipid oxidation in a linoleic acid emulsion, fish flesh and fish feed and in hydroxyl radical(·OH)-treated carp erythrocytes. The linoleic acid, fish flesh and fish feed were incubated with BHT,EQ and EGbs at 45℃ for 8 d, respectively, except for the control group. The lipid oxidation in the linoleic acid emulsion, fish flesh and fish feed was then measured by the ferric thiocyanate method or thiobarbituric acid method. The carp erythrocytes were treated with BHT, EQ. or EGbs in the presence of40 μmol/L FeSO_4 and 20 μmol/L H_2 O_2 at 37℃ for 6 h,except for the control group. Oxidative stress and apoptosis parameters in carp erythrocytes were then evaluated by the commercial kit. The results showed that BHT, EQ and EGbs inhibited lipid oxidation in the linoleic acid emulsion, fish flesh and fish feed and·OH-induced phosphatidylserine exposure and DNA fragmentation(the biomarkers of apoptosis) in carp erythrocytes. Furthermore, BHT, EQ and EGbs decreased the generation of reactive oxygen species(ROS),inhibited the oxidation of cellular components and restored the activities of enzymatic antioxidants in·OH-treated carp erythrocytes. Of all examined EGbs, EAE showed the strongest effects. The effects of EAE on lipid oxidation in the linoleic acid emulsion and on superoxide anion and malonaldehyde levels,catalase activity and apoptosis in. OH-treated carp erythrocytes were equivalent to or stronger than those of BHT. Moreover, these results indicated that the inhibition order of EGbs on the generation of ROS and oxidation of cellular components in fish erythrocytes approximately agreed with that for the food and feed materials tested above. And, the antioxidative and anti-apoptotic effects of EGbs were positively correlated with their flavonoid content. Taken together, these results revealed that the fish erythrocyte system can be used as an experimental model to evaluate lipid oxidation in food and feed ingredients. The EAE can be used as a potential natural antioxidant or apoptosis inhibitor. The inhibition effects of EGbs on lipid oxidation and apoptosis may be due to the presence of flavonoid compounds. | Huatao Li Xiaoqiu Zhou Ping Gao Qiuyue Li Hansi Li Rong Huang Min Wu | 2016 | Animal Nutrition2016，，3： | 6 |
| 4 | The cytotoxicity and protective effects of Astragalus membranaceus extracts and butylated hydroxyanisole on hydroxyl radical-induced apoptosis in fish erythrocytes显示文摘Erythrocytes play an essential role in transporting O_2 and CO_2 for respiration in fish. However,erythrocytes continuously suffer from reactive oxygen species(ROS)-induced oxidative stress and apoptosis. Thus, it is essential to expand our knowledge of how to protect erythrocytes against ROS-induced oxidative stress and apoptosis in fish. In this study, we explored the cytotoxicity and the effects of butylated hydroxyanisole(BHA), ethyl ether extracts, ethyl acetate extracts, acetone extracts(AE), ethanol extracts, and aqueous extracts of Astragalus membranaceus(EAm) on hydroxyl radical(·OH)-induced apoptosis in carp erythrocytes. The rat hepatocytes and carp erythrocytes were incubated with different concentrations of BHA or EAm(0.125 to 1 mg/mL). The toxicity in rat hepatocytes and carp erythrocytes was then measured using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay and a haemolysis assay,respectively. The carp erythrocytes were treated with BHA or EAm in the presence of 40 μmol/L FeSO4 and20 μmol/L H_2 O_2 at 37℃, except for the control group. Oxidative stress and apoptosis parameters in the carp erythrocytes were then evaluated using the commercial kit. The results indicated that at high concentrations, BHA and EAm could induce toxicity in rat hepatocytes and fish erythrocytes. However, BHA was more toxic than EAm at the same concentrations. Moreover, the toxicity order of BHA and EAm in the fish erythrocytes approximately agreed with that for the rat hepatocytes. Butylated hydroxyanisole and EAm suppressed the ·OH-induced phosphatidylserine exposure and DNA fragmentation(the biomarkers of apoptosis) by decreasing the generation of ROS, inhibiting the oxidation of cellular components, and restoring the activities of antioxidants in carp erythrocytes. Of all of the examined EAm, the AE showed the strongest effects. The effects of AE on superoxide anion, H_2 O_2, met-haemoglobin and reduced glutathione levels, as well as glutathione reductase activity and apoptosis were equivalent to or stronger than those of BHA. These results revealed that the AE of Astragalus membranaceus could be used as a potential natural antioxidant or apoptosis inhibitor in fish erythrocytes. | Huatao Li Xiaoqiu Zhou Min Wu Mengling Deng Chao Wang Jingjing Hou Pengju Mou | 2016 | Animal Nutrition2016，，4： | 5 |
| 5 | A practical soil management to improve soil quality by applying mineral organic fertilizer显示文摘Heavy use of chemical fertilizer causes increasing soil and environmental crisis, and the use of organic fertilizer increases obvious in recent years. In this study,mineral organic fertilizer(MOF) and compound fertilizer(CF) were applied in amaranth culture to explore the effects of these two kinds of fertilizers on soil quality and the potential function for CO_2 fixation. Some soil parameters were tested, e.g. p H value, organic carbon content, microbial biomass, urease activity, and available potassium content. In addition, some parameters of soil infiltration water were also determined, such as p H and HCO_3^- concentration. Experimental results showed that MOF improved soil quality and amaranth biomass and increased possible soil carbon sink.On the contrary, the utilization of CF worsened soil quality and made the soil acidize. These results suggested that MOF can partially replace CF to improve plant growth, soil quality and possible CO_2 sink. | Leilei Xiao Qibiao Sun Huatao Yuan Bin Lian | 2017 | Acta Geochimica2017，36，2： | 5 |
| 6 | Identification of key genes affecting porcine fat deposition based on coexpression network analysis of weighted genes显示文摘Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting consumers’choice of pork.Weighted gene co-expression network analysis(WGCNA)is effective in pig genetic studies.Therefore,this study aimed to identify modules that co-express genes associated with fat deposition in pigs(Songliao black and Landrace breeds)with extreme levels of backfat(high and low)and to identify the core genes in each of these modules.Results:We used RNA sequences generated in different pig tissues to construct a gene expression matrix consisting of 12,862 genes from 36 samples.Eleven co-expression modules were identified using WGCNA and the number of genes in these modules ranged from 39 to 3,363.Four co-expression modules were significantly correlated with backfat thickness.A total of 16 genes(RAD9A,IGF2R,SCAP,TCAP,SMYD1,PFKM,DGAT1,GPS2,IGF1,MAPK8,FABP,FABP5,LEPR,UCP3,APOF,and FASN)were associated with fat deposition.Conclusions:RAD9A,TCAP,SMYD1,PFKM,GPS2,and APOF were the key genes in the four modules based on the degree of gene connectivity.Combining these results with those from differential gene analysis,SMYD1 and PFKM were proposed as strong candidate genes for body size traits.This study explored the key genes that regulate porcine fat deposition and lays the foundation for further research into the molecular regulatory mechanisms underlying porcine fat deposition. | Kai Xing Huatao Liu Fengxia Zhang Yibing Liu Yong Shi Xiangdong Ding Chuduan Wang | 2022 | Journal of Animal Science and Biotechnology2022，13，1： | 3 |
| 7 | Effects of Angelica sinensis extracts on lipid oxidation in fish feeds and growth performance of juvenile Jian carp(Cyprinus carpio var. Jian)显示文摘The study was to explore the effect of the extracts of Angelica sinensis(EAs) on lipid oxidation in fish feeds compared with ethoxyquin(EQ) and the effect of dietary EAs on growth performance of carp(Cyprinus carpio var. Jian). Firstly, fish feeds were respectively added with EQ, and ethyl ether extract ethyl acetate extract(EAE), acetone extract, ethanol extract(EE) and aqueous extract(AQE) of Angelica sinensis, except for the control. The results showed that EAs and EQ inhibited lipid oxidation in fish feed(P < 0.05). Of all of the examined EAs, EAE showed the strongest protective effects against lipid oxidation(P < 0.05). Moreover, EAE at high concentrations showed a stronger effect on lipid oxidation compared with EQ(P < 0.05). Then, 7 experimental diets respectively supplemented with 0.0, 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 g/kg of EAE were fed to the respective treatment groups for 30 d. Four replicates were performed for each treatment group; 20 carp(mean weight: 12.10 ± 0.13 g) were in each replicate. The result indicated that dietary EAE improved the growth performance in carp(P < 0.05). The appropriate con centration of EAE for carp growth was estimated to be 3.643 g/kg diet. Thus, EAE could be used as a natural antioxidant in feeds for Jian carp. | Huatao Li Dandan Yang Zhihao Li Mingquan He Fengyi Li Jun Jiang Siyi Tang Peiyuan Peng Wenhao Du Yuting Ma Ying Liu | 2019 | Animal Nutrition2019，，1： | 2 |
| 8 | β-Catenin Mutations Are Frequent in Human Hepatocellular Carcinomas Associated with Hepatitis C Virus Infection显示文摘 | Huatao Huang Hideki Fujii Anna Sankila Betania M. Mahler-Araujo Masanori Matsuda Gieri Cathomas Hiroko Ohgaki | 1999 | The American Journal of Pathology1999，，6： | 2 |
| 9 | 乙醇蒸气与热空气处理抑制杨梅采后腐烂效果显示文摘本文研究了单独使用乙醇蒸气处理(EVT,250或500μL·L-1持续3 h)或者与热空气处理(HAT,48℃持续3 h)结合使用对中国杨梅采后腐烂与微生物生长的作用效果。与对照相比,乙醇蒸气处理和热空气处理均能显著地减少由接种Verticicladiella abietina,Penicillium citrinum或Trichoderma viride所引起的腐烂;以500μL·L-1乙醇蒸气结合热空气处理杨梅的腐烂率最低,并且能够显著地抑制病原菌的孢子萌发和菌丝生长,杨梅果实自然腐烂率和微生物种群数量均为最低,能够很好地保持果品质量,效果好于二者单独使用。 | Kaituo Wang Peng Jin Shuangshuang Tang Haitao Shang Huaijing Rui Huatao Di Yan Cai Yonghua Zheng | 2011 | 保鲜与加工2011，11，1： | 2 |
| 10 | Rethinking phylogeographic structure and historical refugia in the rufous-capped babbler Cyanoderma ruficeps in light of range-wide genetic sampling and paleodistributional reconstructions显示文摘 | Peter A. HOSNER Huatao LIU A. Townsend PETERSON Robert G. MOYLE | 2015 | Current Zoology2015，61，5： | 2 |
| 11 | The influence of temperature on flow-induced forces on quartz-crystal-microbalance sensors in a Chinese liquor identification electronic-nose: three-dimensional computational fluid dynamics simulation and analysis显示文摘An electronic-nose is developed based on eight quartz-crystal-microbalance (QCM) gas sensors in a sensor box, and is used to detect Chinese liquors at room temperature. Each sensor is a highly-accurate and highly-sensitive oscillator that has experienced airflow disturbances under the condition of varying room temperatures due to unstable flow-induced forces on the sensors surfaces. The three-dimensional (3D) nature of the airflow inside the sensor box and the interactions of the airflow on the sensors surfaces at different temperatures are studied by computational fluid dynamics (CFD) tools. Higher simulation accuracy is achieved by optimizing meshes, meshing the computational domain using a fine unstructural tetrahedron mesh. An optimum temperature, 30 ℃, is obtained by analyzing the distributions of velocity streamlines and the static pressure, as well as the flow-induced forces over time, all of which may be used to improve the identification accuracy of the electronic-nose for achieving stable and repeatable signals by removing the influence of temperature. | Qiang LI Yu GU Huatao WANG | 2019 | Applied Mathematics and Mechanics(English Edition)2019，40，9： | 1 |
| 12 | Sensitive determination of trace- metal elements in tea with capillary electrophoresis by using chelating agent 4 - (2 - pyridylazo) resorcinol (PAR) 显示文摘 | Huatao Feng Tianlin Wang Sam Fong | 2003 | Food Chemistry2003，81，： | 1 |
| 13 | IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines显示文摘 | Steven C.L. Ho Muriel Bardor Huatao Feng Mariati Yen Wah Tong Zhiwei Song Miranda G.S. Yap Yuansheng Yang | 2011 | Journal of Biotechnology2011，，1： | 1 |
| 14 | Proteomics insight into the biological safety of transgenic modification of rice as compared with conventional genetic breeding and spontaneous genotypic variation显示文摘 | GONG Chunyan LI Qi YU Huatao | 2012 | Journal of Proteome Research2012，11，5： | 1 |
| 15 | Boundary conditions on a finite grid:Applications with pseudospectral wave propagation显示文摘 | Lees J | 1997 | Geophysics1997，62，5： | 1 |
| 16 | Effects of Zr O2promoter on physic-chemical properties and activity of Co/Ti O2-Si O2Fischer-Tropsch catalysts显示文摘 | Wu Huatao Yang Yong Suo Haiyun | 2015 | Journal of Molecular Catalysis A:Chemical2015，396，： | 1 |
| 17 | Robust hybrid control based on PD and novel CMAC with improved architecture and learning scheme for electric load simulator 显示文摘 | YANG Bo BAO Ran HAN Huatao | 2014 | IEEE Transactions on In- dustrial Electronics2014，61，10： | 1 |
| 18 | Detecting the backfill pipeline blockage and leakage through an LSTM-based deep learning model显示文摘Detecting a pipeline's abnormal status,which is typically a blockage and leakage accident,is important for the continuity and safety of mine backfill.The pipeline system for gravity-transport high-density backfill(GHB)is complex.Specifically designed,efficient,and accurate abnormal pipeline detection methods for GHB are rare.This work presents a long short-term memory-based deep learning(LSTM-DL)model for GHB pipeline blockage and leakage diagnosis.First,an industrial pipeline monitoring system was introduced using pressure and flow sensors.Second,blockage and leakage field experiments were designed to solve the problem of negative sample deficiency.The pipeline's statistical characteristics with different working statuses were analyzed to show their complexity.Third,the architecture of the LSTM-DL model was elaborated on and evaluated.Finally,the LSTM-DL model was compared with state-of-the-art(SOTA)learning algorithms.The results show that the backfilling cycle comprises multiple working phases and is intermittent.Although pressure and flow signals fluctuate stably in a normal cycle,their values are diverse in different cycles.Plugging causes a sudden change in interval signal features;leakage results in long variation duration and a wide fluctuation range.Among the SOTA models,the LSTM-DL model has the highest detection accuracy of98.31%for all states and the lowest misjudgment or false positive rate of 3.21%for blockage and leakage states.The proposed model can accurately recognize various pipeline statuses of complex GHB systems. | Bolin Xiao Shengjun Miao Daohong Xia Huatao Huang Jingyu Zhang | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，8： | 0 |
| 19 | Solvents adjusted pure phase CoCO_(3) as anodes for high cycle stability显示文摘CoCO_(3) with high theoretical capacity has been considered as a candidate anode for the next generation of lithium-ion batteries(LIBs).However,the electrochemical performance of CoCO_(3) itself,especially the cyclic stability at high current density,hinders its application.Herein,pure phase CoCO_(3) particles with different particle and pore sizes were prepared by adjusting the solvents(diethylene glycol,ethylene glycol,and deionized water).Among them,CoCO_(3) synthesized with diethylene glycol(DG-CC)as the solvent shows the best electrochemical performance owing to the smaller particle size and abundant mesoporous structure to maintain robust structural stability.A high specific capacity of 690.7 mAh/g after 1000 cycles was achieved,and an excellent capacity retention was presented.The capacity was contributed by diverse electrochemical reactions and the impedance of DG-CC under different cycles was further compared.Those results provide an important reference for the structural design and stable cycle performance of pure CoCO_(3). | Liming LIU Xiaoxiao HUANG Zengyan WEI Xiaoming DUAN Bo ZHONG Long XIA Tao ZHANG Huatao WANG Dechang JIA Yu ZHOU Rui ZHANG | 2021 | Journal of Advanced Ceramics2021，10，3： | 0 |
| 20 | Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds显示文摘Abscisic acid(ABA)is involved in regulating diverse biological processes,but its signal transduction genes and roles in hemp seed germination are not well known.Here,the ABA signaling pathway members,PYL,PP2C and SnRK2 gene families,were identified from the hemp reference genome,including 7 CsPYL(pyrab-actin resistance1-like,ABA receptor),8 CsPP2CA(group A protein phosphatase 2c),and 7 CsSnRK2(sucrose nonfermenting1-related protein kinase 2).The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage.Exogenous ABA(1 or 10μM)treatment had a significant regulatory effect on the selected PYL,PP2C,SnRK2 gene families.CsAHG3 and CsHAI1 were most significantly affected by exogenous ABA treatment.Yeast two-hybrid experiments were performed to reveal that CsPYL5,CsSnRK2.2,and CsSnRK2.3 could interact with CsPP2CA7 and demonstrate that this interaction was ABA-independent.Our results indicated that CsPYL5,CsSnRK2.2,CsSnRK2.3 and CsPP2CA7 might involve in the ABA signaling transduction pathway of hemp seeds during the hemp seed germination stages.This study suggested that novel genetic views can be brought into investigation of ABA signaling pathway in hemp seeds and lay the foundation for further exploration of the mechanism of hemp seed germination. | Cong Hou Kang Ning Xiuye Wei Yufei Cheng Huatao Yu Haibin Yu Xia Liu Linlin Dong | 2023 | Phyton-International Journal of Experimental Botany2023，92，7： | 0 |
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