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| 1 | 凹凸棒石-TiO_2纳米复合材料制备和表征显示文摘具有高吸附性能的载体-TiO2光催化剂复合材料制备是未来TiO2纳米光催化研究领域的重要发展方向。本文以凹凸棒石为载体,通过钛酸四丁酯-丙醇溶液浸渍-过滤-丙醇蒸发-水蒸气作用下钛酸四丁酯水解-低温煅烧程序操作,获得凹凸棒石-TiO2纳米复合光催化材料。高分辨透射电镜表征结果显示:TiO2颗粒直径5~10nm,具有锐钛矿结构,在凹凸棒石表面分布均匀。该制备方法工艺简单,钛酸四丁酯利用率高,对水中有机污染物光催化处理、抗菌消毒、空气净化等方面具有很大潜在应用价值。 | 陈天虎 史晓莉 彭书传 XU Huifang Gao Huizhen | 2005 | 硅酸盐通报2005，24，1： | 31 |
| 2 | The recommendations of Chinese Parkinson’s disease and movement disorder society consensus on therapeutic management of Parkinson’s disease显示文摘Background:Parkinson’s disease(PD)is a chronic,progressive and debilitating disease,which affects over 2.5 million people in China.PD is characterized clinically by resting tremor,muscular rigidity,bradykinesia and postural instability.As the disease progresses,additional complications can arise such as non-motor and neurobehavioral symptoms.Pharmacological treatment and surgical intervention for PD have been implemented in China.Until 10 years ago,there was lack of standardization for the management of PD in different regions and among different physicians,leading to different treatment levels in different regions and different physicians.Since then,the Chinese Parkinson’s Disease and Movement Disorder Society have published three versions of guidelines for the management of PD in China,in 2006,2009 and 2014,respectively.Correspondingly,the overall level of treatment for PD in China improved.Objectives:To update the treatment guidelines based on current foreign and domestic practice guidelines and clinical evidence,and to improve the treatment options available to physicians in the management of PD.Summary:A variety of treatment recommendations in the treatment guidelines have been proposed,including physical activity and disease-modifying medication,which should be initiated at the early-stage of the disease.The principles of dosage titration should be followed to avoid acute adverse reactions to the drugs,to achieve a satisfactory clinical effect with a low dose and to reduce the incidence of long-term motor complications.Moreover,different treatment strategies should be considered at different stages of the disease.Importantly,treatment guidelines and personalized treatments should be valued equally.A set of treatment recommendations has been developed to assist physicians to improve and optimize clinical outcomes for patients with PD in China. | Shengdi Chen Piu Chan Shenggang Sun Haibo Chen Baorong Zhang Weidong Le Chunfeng Liu Guoguang Peng Beisha Tang Lijuan Wang Yan Cheng Ming Shao Zhenguo Liu Zhenfu Wang Xiaochun Chen Mingwei Wang Xinhua Wan Huifang Shang Yiming Liu Pingyi Xu Jian Wang Tao Feng Xianwen Chen Xingyue Hu Anmu Xie Qin Xiao | 2016 | Translational Neurodegeneration2016，5，1： | 23 |
| 3 | 凹凸棒石-银纳米复合抗菌材料制备方法和表征显示文摘凹凸棒石是具有链层状晶体结构镁铝硅酸盐粘土矿物,呈现直径约30~40nm、长度可达数微米的棒状晶体形态,是产出丰富的天然纳米矿物材料,表现出纳米效应、吸附活性和化学活性,是一种重要的载体材料。本文研究了凹凸棒石-Ag纳米复合抗菌材料制备方法和抗菌效果。高分辨透射电镜(HRTEM)表征结果表明:用丙胺基三乙基硅烷为表面活性剂,醌醇为还原剂,凹凸棒石硝酸银浸渍法制备的复合材料,纳米银颗粒为1~7nm,均匀分布在凹凸棒石表面,复合抗菌材料含银0.52%。抗菌圈实验显示较好的抗菌效果,悬浮液浊度法抗菌实验显示凹凸棒石-Ag纳米复合抗菌剂最低抗菌浓度为0.47g/L。 | 陈天虎 李宏伟 汪家权 施晶俊 Xu Huifang Gao Huizhen | 2005 | 硅酸盐通报2005，24，2： | 17 |
| 4 | 黄土中强磁性矿物透射电子显微镜观察和成因分析显示文摘透射电子显微镜(TEM)分析结果表明,黄土-古土壤序列中强磁性矿物包括磁铁矿和磁赤铁矿.原生磁铁矿具有风成碎屑颗粒物形貌和表面特征,又可以分为高钛和低钛两种类型,可能分别指示岩浆岩和变质岩源区.TEM纳米尺度观察研究证实,部分原生微米粒级碎屑磁铁矿在成壤过程中风化成5~20 nm磁赤铁矿,并保持风成碎屑磁铁矿的假象形态.部分绿泥石在成壤风化作用过程中形成纳米磁铁矿或磁赤铁矿.这两种矿物的风化作用都形成超顺磁颗粒,可能是古土壤磁化率增强的重要机制.部分绿泥石风化形成的磁铁矿或磁赤铁矿含有少量的磷和硫,具生物成因标志,表明微生物可能对绿泥石的风化及超顺磁颗粒形成起到一定作用. | 陈天虎 XU Huifang 季峻峰 陈骏 陈旸 | 2003 | 科学通报2003，48，17： | 13 |
| 5 | 苏皖坡缕石粘土中蛋白石特征及其成因意义显示文摘TEM和XRD研究表明,在苏皖坡缕石粘土矿床的蛋白石坡缕石层中存在opal A和opal CT两种结构的蛋白石。蛋白石坡缕石层主要含坡缕石、opal A、opal CT,以及少量白云石和其它矿物,由富opal A层和富opal CT层互层组成,交互层的厚度在几个厘米左右。蛋白石坡缕石层中的矿物基本是自生矿物,从蒸发湖水中化学沉淀形成。矿物组成特征研究表明,蛋白石坡缕石层的矿物组分(Si、Mg、Al)来源于盆地周围玄武岩淋滤的浅层地下水。根据Opal A和Opal CT溶解度图解和坡缕石、白云石形成物理化学条件图解,当湖水具有高浓度溶解SiO2和Mg2+时,有利于opal A和坡缕石形成,当湖水具有低浓度溶解SiO2时,有利于opal CT结晶。因此,沉淀SiO2的结构状态取决于地下水补给的湖水溶解SiO2浓度。富opal A和富opal CT交互层的形成是古气候、古水文周期性变化的指示。富opal CT层指示高地下水补给流入量,低蒸发量,湖水低盐度和溶解组分,代表湿润气候时期;而富opal A层代表低地下水补给流入量,高蒸发量,高溶解组分浓度,代表干旱气候时期。 | 陈天虎 徐晓春 Huifang Xu 岳书仓 | 2005 | 矿物学报2005，25，1： | 8 |
| 6 | 镁铝阴离子粘土对砷酸根离子的吸附作用显示文摘利用共沉淀水热法制备了镁铝阴离子粘土材料,研究了其水溶液对砷酸根离子的去除能力。结果表明:镁铝阴离子粘土对砷酸根离子具有较高的去除率和吸附容量、较快的吸附速度。砷酸根离子的吸附平衡数据很好的符合Freundlich型吸附等温方程。同时,吸附动力学数据完全符合Lagergren二级速度方程。吸附砷酸根离子后的阴离子粘土材料,用稀Na2CO3溶液可以很好的洗脱再生。阴离子粘土对砷酸根离子的吸附属于层间离子交换作用。 | 彭书传 杨远盛 陈天虎 姜绍通 XU Huifang | 2005 | 硅酸盐学报2005，33，8： | 8 |
| 7 | Nano-sized graphitic carbon in authigenic tube pyrites from offshore southwest Taiwan, South China Sea, and its implication for tracing gas hydrate显示文摘Gas hydrates are a significant energy resource and are usually detected by bottom simulating reflection and submarine geochemical anomalies. Authigenic minerals are related to gas hydrates, with carbonates, sulfates and sulfides being important tracing minerals. Authigenic tubular pyrites were collected from offshore southwest Taiwan in the South China Sea, and were investigated by scanning electron microscopy(SEM) and high-resolution transmission electron microscopy (HRTEM). Authigenic tubular pyrite was composed of framboidal pyrite, within which nanosized graphitic carbon of low crystallinity was discovered. The graphitic carbon coexisted with pyrite and had a texture similar to carbon nanotubes and nanocones, indicating that they likely precipitated from carbon supersaturated C-H-O fluid. Pyrite may act as a catalyst for the conversion of CH 4 to C. The discovery of nanosized graphitic carbon in pyrite indicated it was deposited in sediments that were supersaturated with methane fluid. Thus, nanosized graphitic carbon may be another tracing species for submarine gas hydrates. The discovery of nanosized graphitic carbon deposited in a low temperature environment will enlighten our understanding of the laboratory synthesis and industrial production of graphitic carbon. | ZHANG Mei SUN XiaoMing XU Li XU HuiFang KONISHI Hirom LU Yang LU HongFeng WU ZhongWei | 2011 | Chinese Science Bulletin2011，56，19： | 8 |
| 8 | Structural Evolvement of Heating Treatment of Mg/AI-LDH and Preparation of Mineral Mesoporous Materials显示文摘Although hydrotalcite, or layered double hydroxides (LDHs), is not a common mineral, it is an important material that can be easily synthesized in laboratory. In this study, structural evolvement and BET surface area changes of heat treated Mg/Al-LDH is evaluated by XRD, TEM and N2-BET analyses. The results indicate that the magnesium-aluminum LDH with carbonate as interlayer anion, periclase-like oxides was formed at temperatures of 400-800℃. Meanwhile, 2-3 nanometer mesoporous were formed during decomposition of LDH. However, the heat treated samples still preserve the morphology of the original LDH plates. Periclase-like formed from LDH heat treatment may re-hydrolyze and recover the structure of LDH. However, crystallinity of the recovered LDH is lower than that of the original LDH. This heat treatment will result in formation of (Mg, Al)-oxide nano-crystals and nanopores among the nano-crystals. When heating temperature exceeds 1000, the periclase-like (Mg, Al)-oxide is transformed into a composite with periclase (MgO) and spinel phases. The periclase can be re-hydrolyzed and dissolved in HCl solution. After acid treatment, the sample with a high surface area is composed of spinel nano-crystals and nanopores among them. Our results will provide a new and economic way to synthesize mesoporous materials through pathways of phase transformation of precursor materials with different composition. | CHEN Tianhu XU Huifang WANG Yifeng QING Chengsong FAN Mingde CHEN Gang | 2006 | Acta Geologica Sinica(English Edition)2006，80，2： | 5 |
| 9 | 南海台西南盆地自生管状黄铁矿中纳米级石墨碳的发现及其对天然气水合物的示踪意义显示文摘海底天然气水合物是十分重要的能源矿产,目前主要是根据似海底反射面(BSR)等地球物理方法和海底地球化学异常示踪其存在.此外,与天然气水合物有关的自生矿物如碳酸盐、硫酸盐和硫化物等矿物也是重要的示踪体系.本文利用扫描电子显微镜(SEM)和高分辨率透射电子显微镜(HRTEM),对来自南海台西南盆地沉积物中的自生管状黄铁矿进行了系统的观测,发现它们主要由草莓状黄铁矿组成,且在草莓状黄铁矿中首次发现了纳米级的低结晶度石墨碳,它们主要呈现出似纳米碳管和纳米锥形状,而且与黄铁矿密切共生,显示它们可能主要形成于含C过饱和C–H–O流体的沉淀.黄铁矿在CH4转变为原子C的过程中起催化作用.自生管状黄铁矿中新发现的纳米级石墨碳,显示其沉积时沉积岩围岩中存在CH4过饱和流体,因此可作为天然气水合物又一重要示踪矿物.此外,低温环境中纳米石墨碳的发现对石墨的实验室合成和工业生产等有借鉴意义. | 张美 孙晓明 徐莉 XU HuiFang KONISHI Hiromi 芦阳 陆红锋 吴仲玮 | 2011 | 科学通报2011，56，21： | 4 |
| 10 | Excess TNF-α in the blood activates monocytes with the potential to directly form cholesteryl ester-laden cells显示文摘肿瘤坏死因素 --(TNF-) 并且 monocytic 房间在动脉粥样硬化的发展起一个关键作用,它是冠的心疾病(CHD ) 的主要原因。在这个工作,我们在血在单核白血球上调查了过量 TNF- 的效果并且发现从 CHD 病人的血单核白血球有潜力直接形成 cholesteryl 酉旨(CE ) 沉重的房间在下面在里面有 oxLDL 的 vitro 孵化。proinflammatory cytokines 的血浆层次例如 TNF- , interleukin 6 (IL-6 ) ,并且 C 反应蛋白质(CRP ) ,在 CHD,病人们是比在控制的那些显著地高级的健康志愿者。然而, TNF- ,然而并非 IL-6 或 CRP 的仅仅血浆水平,断然与血单核白血球的潜力被相关直接形成 CE 沉重的房间。由使用人的血单核白血球和 monocytic THP-1 房间, CE 沉重的房间的形成上的 TNF- 的激活的效果被表明,它能被 anti-TNF- 抗体明确地堵住。而且, TNF- 能由分别地提高功能的粘附分子和 scavenger 受体的表示增加粘附和单核白血球的 oxLDL 举起,这也被揭示。最后,结果在 vivo 并且在 vitro,与一个老鼠模型一起的实验证实在血的过量 TNF- 与潜力激活单核白血球直接形成 CE 沉重的房间。这些数据证明在血的过量 TNF- 是为动脉粥样硬化和 CHD 的开发的主要扳机。 | Ming Zhu Lei Lei Zhenhua Zhu Qin Li Dongqing Guo Jiajia Xu Jia Chen Huifang Sha Xiaowei Zhang Xinying Yang Baoliang Song Boliang Li Yan Yan Ying Xiong | 2015 | Acta Biochimica et Biophysica Sinica2015，47，11： | 4 |
| 11 | Coking-resistant Ni-ZrO_2/Al_2O_3 catalyst for CO methanation显示文摘Highly coke-resisting Zr O2-decorated Ni/Al2O3 catalysts for CO methanation were prepared by a two-step process. The support was first loaded with Ni O by impregnating method and then modified with Zr O2 by deposition-precipitation method(IM-DP). Nitrogen adsorptiondesorption, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, thermogravimetric analysis, H2 temperatureprogrammed reduction and desorption, NH3temperature-programmed desorption, and zeta potential analysis were employed to characterize the samples. The results revealed that, compared with the catalysts with the same composition prepared by co-impregnation(CI) and sequential impregnation(SI) methods, the Ni/Al2O3 catalyst prepared by IM-DP showed much enhanced catalytic performance for syngas methanation under the condition of atmospheric pressure and a high weight hourly space velocity of 120000 m L g-1 h-1. In a 80 h life time test under the condition of 300–600°C and 3.0 MPa, this catalyst showed high stability and resistance to coking, and the amount of deposited carbon was only 0.4 wt%. On the contrary, the deposited carbon over the catalyst without Zr O2 reached 1.5 wt% after a 60 h life time test. The improved catalytic performance was attributed to the selective deposition of Zr O2 nanoparticles on the surface of Ni O rather than Al2O3, which could be well controlled via changing the electrostatic interaction in the DP procedure. This unique structure could enhance the dissociation of CO2 and generate surface oxygen intermediates, thus preventing carbon deposition on the Ni particles in syngas methanation. | Qing Liu Fangna Gu Jiajian Gao Huifang Li Guangwen Xu Fabing Su | 2014 | Journal of Energy Chemistry2014，23，6： | 4 |
| 12 | 南海台西南盆地自生管状黄铁矿及其对天然气水合物的指示意义显示文摘 | 张美 孙晓明 芦阳 徐莉 XU Huifang | 2009 | 矿物学报2009，29，S1： | 4 |
| 13 | Polarization-independent all-silicon dielectric metasurfaces in the terahertz regime显示文摘Dielectric metasurfaces have achieved great success in realizing high-efficiency wavefront control in the optical and infrared ranges. Here, we experimentally demonstrate several efficient, polarization-independent, all-silicon dielectric metasurfaces in the terahertz regime. The metasurfaces are composed of cylindrical silicon pillars on a silicon substrate, which can be easily fabricated using etching technology for semiconductors. By locally tailoring the diameter of the pillars, full control over abrupt phase changes can be achieved. To show the controlling ability of the metasurfaces, an anomalous deflector, three Bessel beam generators, and three vortex beam generators are fabricated and characterized. We also show that the proposed metasurfaces can be easily combined to form composite devices with extended functionalities. The proposed controlling method has promising applications in developing low-loss, ultra-compact spatial terahertz modulation devices. | HUIFANG ZHANG XUEQIAN ZHANG QUAN XU QIU WANG YUEHONG XU MINGGUI WEI YANFENG LI JIANQIANG GU ZHEN TIAN CHUNMEI OUYANG XIXIANG ZHANG CONG HU JIAGUANG HAN WEILI ZHANG | 2018 | Photonics Research2018，9，1： | 4 |
| 14 | Magnetic beads-based multicolor colorimetric immunoassay for ultrasensitive detection of aflatoxin B_(1)显示文摘Aflatoxin B_(1)(AFB_(1))is one of the most toxic,mutagenic and carcinogenic mycotoxin,widely exists in contaminated food,grains and feedstuff products.In this study,a novel magnetic beads multicolor colorimetric immunoassay(MBMCIA)based on Au@Ag nanorods(Au@Ag NRs)is proposed to visual detect ultralow concentration of AFB_(1)with high-resolution by the naked-eye.To design the MBMCIA system,AFB_(1)-BSA conjugates were first coated on the surface of magnetic beads(MBs),then alkaline phosphatase(ALP)as a bridge between immunoassay a nd color reaction was used for catalytic hydrolysis of ascorbic acid-phosphate to generate reductive ascorbic acid.Finally,the yielded ascorbic acid could reduce silver ions to grow a silver coating on the surface of gold nanorods to generate Au@Ag NRs,which leads to the bule-shifted longitudinal absorption peak of Au NRs,accompanying with a series of perceptible color change.Under the optimal conditions,the proposed MBMCIA exhibited go od sensitivity and specificity for the detection of AFB_(1)with the detection limit as low as 5.7 pg/mL Meanwhile,the MBMCIA was also applied for the analysis of AFB_(1)in spiked wheat samples,the obtained recoveries range from 99.1%to 104.3%with relative standard deviation(RSD)less than 7.05%were acceptable.The proposed MBMCIA integrates separated,enriched,anti-interfe rence and signal read-out into one,which opens up a new avenue for an on-site visual food safety inspection or environmental monitoring. | Shan He Qitong Huang Ying Zhang Huifang Zhang Huifeng Xu Xun Li Xiaoming Ma | 2021 | Chinese Chemical Letters2021，32，4： | 3 |
| 15 | Short Text Mining Framework with Specific Design for Operation and Maintenance of Power Equipment显示文摘In order to recover the value of short texts in the operation and maintenance of power equipment,a short text mining framework with specific design is proposed.First,the process of the short text mining framework is summarized,in which the functions of all the processing modules are introduced.Then,according to the characteristics of short texts in the operation and maintenance of power equipment,the specific design for each module is proposed,which adapts the short text mining framework to a practical application.Finally,based on the framework with the specific designed modules,two examples in terms of defect texts are given to illustrate the application of short text mining in the operation and maintenance of power equipment.The results of the examples show that the short text mining framework is suitable for operation and maintenance tasks for power equipment,and the specific design for each module is beneficial for the improvement of the application effect. | Huifang Wang Ziquan Liu Yongjin Xu Xiaoxiong Wei Lixin Wang | 2021 | CSEE Journal of Power and Energy Systems2021，7，6： | 3 |
| 16 | Applying DNA Barcoding to Identify Actinidia in the Northeast of China显示文摘Identification of kiwifruit germplasm materials is the basis of protecting and utilizing these resources. However,identifying Actinidia arguta varieties based on morphology is difficult,especially for non-specialists. In this study,we collected 180 specimens comprising 60 varieties of three species( A. arguta,A. kolomikta,and A. polygama) from the Northeast of China. The emphasis of our study was on the feasibility of identifying A. arguta varieties. Here,we used common analysis methods( genetic distance,phylogenetic analysis and the DNA barcoding gap) and SNPs analysis to evaluate the discriminatory power of different DNA barcoding markers. The results revealed that common methods were insufficient to identify A. arguta varieties but SNPs analysis based on DNA barcoden was the potential method for identifying A. arguta varieties. Besides,Our study agree that DNA barcoding could be used to analysis the evaluation genetic relationship of the Actinidia plants. | haishuang liu hongyan qin jun ai huifang song yiming yang shutian fan peilei xu guangli shi changyu li | 2018 | Agricultural Biotechnology2018，7，2： | 2 |
| 17 | Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis显示文摘The adenoma-carcinoma sequence is a well-accepted roadmap for the development of sporadic colorectal cancer.However,cellular heterogeneity in aberrant epithelial cells limits our understanding of carcinogenesis in colorectal tissues.Here,we performed a single-cell RNA sequencing survey of 54,788 cells from patient-matched tissue samples,including blood,normal tissue,para-cancer,polyp,and colorectal cancer. | Xiaobo Zheng Jinen Song Chune Yu Zongguang Zhou Xiaowei Liu Jing Yu Guangchao Xu Jiqiao Yang Xiujing He Xin Bai Ya Luo Yu Bao Huifang Li Lie Yang Mingqing Xu Nan Song Xiaodong Su Jie Xu Xuelei Ma Hubing Shi | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 2 |
| 18 | Transformation relationship among different magnetic minerals within loess-paleosol sediments of the Chinese Loess Plateau显示文摘The dominant magnetic minerals and carriers of magnetic signals within the Chinese Loess Plateau are magnetite, maghemite, hematite, and goethite. In this study, we investigated the provenance and evo- lution of magnetic minerals during loess pedogenesis, using X-ray diffraction (XRD) and optical and electron microscopy, including field emission scanning electron microscopy (FESEM) and high- resolution transmission electron microscopy (HRTEM). Our results reveal that single- and multiphase mineral assemblages among magnetic minerals in the loess-paleosol sequence have been formed. Partial oxidation of coarse eolian magnetite has occurred in the desert source area and the oxidation degree is enhanced after deposition of the dust upon the Chinese Loess Plateau. This mode of origin resulted in a microtexture consisting of an inner magnetite core surrounded by a hematite rim, and strongly affected the magnetic characteristics of the loess. Goethite coexists with hematite in the loess and paleosol, and nanometer-scale hematite is formed upon goethite rims via dehydration. Our study provides direct mineralogical evidence of the magnetic record and paleoclimatic implications of the loess–paleosol sequence of the Chinese Loess Plateau. | XIE QiaoQin CHEN TianHu XU XiaoChun QING ChengSong XU HuiFang SUN YuBing JI JunFeng | 2009 | Science China Earth Sciences2009，52，3： | 2 |
| 19 | Four-Beam Model for Vibration Analysis of a Cantilever Beam with an Embedded Horizontal Crack显示文摘As one of the main failure modes, embedded cracks occur in beam structures due to periodic loads. Hence it is useful to investigate the dynamic characteristics of a beam structure with an embedded crack for early crack detection and diagnosis. A new four-beam model with local flexibilities at crack tips is developed to investigate the transverse vibration of a cantilever beam with an embedded horizontal crack; two separate beam segments are used to model the crack region to allow opening of crack surfaces. Each beam segment is considered as an Euler-Bernoulli beam. The governing equations and the matching and boundary conditions of the four-beam model are derived using Hamilton's principle. The natural frequencies and mode shapes of the four-beam model are calculated using the transfer matrix method. The effects of the crack length, depth, and location on the first three natural frequencies and mode shapes of the cracked cantilever beam are investigated. A continuous wavelet transform method is used to analyze the mode shapes of the cracked cantilever beam. It is shown that sudden changes in spatial variations of the wavelet coefficients of the mode shapes can be used to identify the length and location of an embedded horizontal crack. The first three natural frequencies and mode shapes of a cantilever beam with an embedded crack from the finite element method and an experimental investigation are used to validate the proposed model. Local deformations in the vicinity of the crack tips can be described by the proposed four-beam model, which cannot be captured by previous methods. | LIU Jing ZHU Weidong CHARALAMBIDES Panos G SHAO Yimin XU Yongfeng WU Kai XIAO Huifang | 2016 | Chinese Journal of Mechanical Engineering2016，29，1： | 2 |
| 20 | Elevated miR-10a-5p facilitates cell cycle and restrains adipogenic differentiation via targeting Map2k6 and Fasn,respectively显示文摘miRNAs are a small class of noncoding RNAs that perform biological functions by regulating the stability or translation of target genes in various biological processes.This study illustrated the role of miR-10a-5p,which is relatively enriched in adipose tissues,using primary mouse preadipocytes as model.With elevated miR-10a-5p expression,the proliferative ability of mouse preadipocytes was significantly enhanced,indicated by increased EdU+cells and G1/S transition,accompanied by upregulated Cyclin B,Cyclin D and PCNA and downregulated p21 and p27.Meanwhile,the adi-pogenic differentiation was significantly attenuated by elevated miR-10a-5p,supported by Oil Red 0 staining and suppressed PPARγand aP2 expression.Furthermore,Map2k6 and Fasn were pre-dicted to be the target genes of miR-10a-5p in silico,and dual luciferase reporter assay confirmed the direct targeting effects.Western blot analysis results showed that miR-10a-5p specially reduced Map2k6 expression at the proliferative stage without affecting Fasn expression,while significantly restrained Fasn expression with unchanged Map2k6 expression during adipogenic differentiation.Taken together,these results revealed a potential role of miR-10a-5p in adipogenesis and in the treatment of obesity. | Xiaoyu Wang Huifang Zhang Meixue Xu Xin'E Shi Gongshe Yang Shiduo Sun Xiao Li | 2020 | Acta Biochimica et Biophysica Sinica2020，52，11： | 2 |
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