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| 1 | Protein trans-splicing based dual-vector delivery of the coagulation factor Ⅷ gene显示文摘A dual-vector system was explored for the delivery of the coagulation factor VIII gene,using intein-mediated protein trans-splicing as a means to produce intact functional factor VIII post-translationally.A pair of eukaryotic expression vectors,expressing Ssp DnaB intein-fused heavy and light chain genes of B-domain deleted factor VIII (BDD-FVIII),was constructed.With transient co-transfection of the two vectors into 293 and COS-7 cells,the culture supernatants contained (137±23) and (109±22) ng mL–1 spliced BDD-FVIII antigen with an activity of (1.05±0.16) and (0.79±0.23) IU mL–1 for 293 and COS-7 cells,respectively.The spliced BDD-FVIII was also detected in supernatants from a mixture of cells transfected with inteinfused heavy and light chain genes.The spliced BDD-FVIII protein bands from cell lysates were visualized by Western blotting.The data demonstrated that intein could be used to transfer the split factor VIII gene and provided valuable information on factor VIII gene delivery by dual-adeno-associated virus in hemophilia A gene therapy. | ZHU FuXiang,LIU ZeLong,CHI XiaoYan & QU HuiGe Life Science College of Ludong University,Yantai 264025,China | 2010 | Science China(Life Sciences)2010，53，6： | 27 |
| 2 | The effect of a secretion-enhanced heavy chain on improving intein-based dual-vector co-delivery of a full-length factor Ⅷ gene显示文摘Treatment of hemophilia A by gene therapy is adversely affected by inefficient FVIII secretion and the large FVIII gene,which is difficult to package in the promising adeno-associated virus (AAV) vectors.Inhibited secretion of FVIII is caused mainly by inefficient secretion of its heavy chain.Previously,we have employed a protein splicing-based dual-vector to co-transfer a B-domain-deleted FVIII (BDD-FVIII) gene,suggesting that the light chain,covalently ligated to a co-expressed heavy chain can improve the secretion of spliced BDD-FVIII.However,its level of secretion was affected by inefficient secretion the heavy chain.Here,we studied the effect of a mutant heavy chain with L303E/F309S substitutions,which enhance FVIII secretion on the heavy chain itself and spliced FVIII when using a protein splicing-based split-delivery of a full-length FVIII gene.Eukaryotic vectors expressing Ssp DnaB intein-fused mutant heavy and light chains were transiently co-transfected into cultured COS-7 cells.A spliced FVIII protein was seen in co-transfected cells by Western blot analysis.The heavy chain was secreted by cells transfected with the mutant heavy chain gene alone at (39±11) ng/mL and this secretion increased to (123±13) ng/mL when cells were co-transfected with the light chain gene,which was greater than the secretion of wild-type heavy chain.The amount of spliced FVIII in the culture supernatant of co-transfected cells was (86±14) ng/mL,with an activity of (0.61±0.08) IU/mL,which was greater than that of wild-type FVIII co-transfected cells.Spliced FVIII and bioactivity were also detected in the combined culture supernatant of cells individually transfected with mutant heavy and light chain gene at a higher level than that of combined wild-type heavy and light chain transfections.This suggested that the heavy chain with improved secretion markedly increased the efficacy of protein splicing-based split delivery of the full-length FVIII gene using a dual-vector.These results encourage the transfer of this technology to an animal model using a dual-AAV vector. | ZHU FuXiang YANG ShuDe LIU ZeLong MIAO Jing QU HuiGe CHI XiaoYan | 2011 | Chinese Science Bulletin2011，56，2： | 8 |
| 3 | Primary research on neoplasm needle track implantation metastasis after radioactive seeds implantation and preventive measures显示文摘Objective:To observe the possibility of neoplasm needle track implantation after radioactive seeds implantation and seek preventive measures to avoid it.Methods:Superficial tissue of 250 seeding needle cores and 250 stylophores employed in neoplasm radioactive seeds implantation was smeared on slides to search for tumor cells.All patients received chemotherapy or endocrine therapy after operations.Ultrasound B-mode or computer tomography(CT)was performed at 10th day,30th day,60th day,and 180th day post operation to detect neoplasm implantation metastasis through needle tracks. Results:Positive cells were found on 13 of 250(5.20%)cores,and 7 of 250(2.80%)stylophores.The difference was not sta- tistically significant(P>0.05).The positive cells frequency of needles those traversed distance less than 3 cm in normal tissue was 6.19%(13/210),while the frequency of the others those traversed longer distance in normal tissue was 2.41%(7/290). The positive cells frequency of needles traversing different distances in normal tissues is significantly different(P<0.05).No neoplasm was detected through needle tracks by ultrasound B-mode or CT in 180 days after operation.Conclusion:Tumor cells could ablate into the needle track during radioactive seed implantation.Some preventive measures,such as optimization of pre-operation and intra-operation treatment plan,chemotherapy or endocrine therapy after operation,may be beneficial to avoid the implantation metastasis of neoplasm in seeding needle tracks. | Juan Wang Weihong Gong Huige Fan Aixia Sui Na Zhao Yongqing Shen | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，4： | 5 |
| 4 | Risk of Lung Cancer and Occupational Exposure to Polycyclic Aromatic Hydrocarbons Among Workers Cohorts—Worldwide,1969–2022显示文摘Summary What is already known about this topic?Lung cancer has a high mortality,resulting in a severe disease burden.Polycyclic aromatic hydrocarbons(PAHs)are definitive carcinogen to human,and occupational exposure to PAHs is associated with lung cancer.What is added by this report?We analyzed the cancer cases from cohort studies on various PAHs exposed workers in China and other countries,calculated the quantitative risk of lung cancer based on meta-analyses,and confirmed the increased risk from lung cancer in selected PAHs exposed occupations.What are the implications for public health practices?There is a clear need to prevent lung cancer on a wide range of PAHs-related occupations in China and around the world.It is crucial to establish guidelines for improving the monitoring on exposure and health promotion in related working environments. | Huige Yuan Yanhua Wang Huawei Duan | 2022 | China CDC weekly2022，4，17： | 3 |
| 5 | An efficient pairing-free certificateless signature scheme for resource-limited systems显示文摘Appendix A Introduction With the development of computation and communication,resource-limited systems have been quite widespread,such as wireless sensor networks(WSNs)[1,2],PDAs,cell phones and smartcards.However,these systems cannot execute | Liangliang Wang Kefei Chen Yu Long Huige Wang | 2017 | Science China(Information Sciences)2017，60，11： | 3 |
| 6 | Enhanced plasma factor Ⅷ activity in mice via cysteine mutation using dual vectors显示文摘Hemophilia A is caused by a genetic mutation in coagulation factor Ⅷ(FⅧ) gene and gene therapy is considered to be a promising strategy for its treatment.We recently demonstrated that co-delivery of two vectors expressing M662C mutated heavy and D1828C mutated light chain genes of B-domain-deleted coagulation factor Ⅷ(BDD-FⅧ) leads to inter-chain disulfide cross-linking and improved heavy chain secretion in vitro.In this study,co-injection of both M662C and D1828C mutated BDD-FⅧ gene expression vectors into mice resulted in increased heavy chain secretion and coagulation activity in plasma in vivo.Approximately(239±56) ng mL 1 above endogenous levels of transgenic FⅧ heavy chain was found in mouse plasma using a chain-specific ELISA.For FⅧ coagulation activity,approximately(1.09±0.25) IU mL 1 above endogenous levels were detected in co-injected transgenic mouse plasma using a chromogenic assay.These data demonstrate that inter-chain disulfide bonds likely increase heavy chain secretion and coagulation activity in the plasma of transgenic mice with an improved efficacy of a dual-vector delivery of BDD-FⅧ gene.These findings support our ongoing efforts to develop a gene therapy for hemophilia A treatment using dual-AAV vectors. | ZHU FuXiang LIU ZeLong MIAO Jing QU HuiGe CHI XiaoYan | 2012 | Science China(Life Sciences)2012，55，6： | 2 |
| 7 | Thermal Stability,Thermal Decomposition and Mechanism Analysis of Cycloaliphatic Epoxy/4,4'-dihydroxydiphenylsulfone/Aluminum Complexes Latent Resin Systems显示文摘The thermal stability of latent resin systems, cycloaliphatic epoxy/4,4'-dihydroxydiphenylsulfone/aluminum complexes, was investigated by dynamic differential scanning calorimetry (DSC) analysis. Experiments were conducted under non-isothermal condition in a nitrogen atmosphere at the heating rate of 10, 20, 30 and 40 ℃/min, respectively. TG curves showed that, in the temperature range of 25 to 600 ℃, the stability of the resin systems could be enhanced by increasing the length of the aliphatic chain in the initiator. Both the Kissinger method and the Ozawa-Flynn-Wall method were employed to calculate activation energies of the decomposition reaction, and the values obtained from the two methods were compared. Moreover, the corresponding reaction mechanism was identified by the Achar differential method and the Coats-Redfern integral method. The experimental results showed that these four methods were reliable and effective to study the kinetics of the thermal decomposition reaction; and the most probable thermal decomposition mechanism of the resin systems we proposed was found to comply with Mampel power law (m=1). | 吴松全 LIU Shaozhu 刘宇艳 ZHU Qi WEI Huige | 2012 | Journal of Wuhan University of Technology(Materials Science)2012，27，6： | 2 |
| 8 | Characterization of stipe and cap powders of mushroom ( Lentinus edodes ) prepared by different grinding methods显示文摘 | Zipei Zhang Huige Song Zhen Peng Qingnan Luo Jian Ming Guahua Zhao | 2011 | Journal of Food Engineering2011，，3： | 2 |
| 9 | Production of large crystals by continuous ripening in a stirred tank显示文摘 | HUIGE N J J and THIJSSEN H A C | 1972 | Crystal Growth1972，，1314： | 1 |
| 10 | Electrochromic polyaniline/graphite oxide nanocomposites with endured electrochemical energy storage显示文摘 | Huige Wei Jiahua Zhu Shijie Wu Suying Wei Zhanhu Guo | 2013 | Polymer2013，，7： | 1 |
| 11 | Activation of protein kinase C α and/or ε enhances transcription of the human endothelial nitric oxide synthase gene 显示文摘 | HUIGE L OEHRLEIN SA WALLERATH T | 1998 | J Mol Pharmacol1998，53，： | 1 |
| 12 | Experimental study of epoxy resin repairing of cracks in fractured rocks显示文摘 | Xing Huige Yang Xingguo Dang Yuhui | 2015 | Polymers&Polymer Composites2015，22，5： | 1 |
| 13 | Production of large crystals by continuous ripening in a stirred tank显示文摘 | Huige N J J Thijssen H A C | | 0，，13： | 1 |
| 14 | Molecular mechanisms of the cardiovascular protective effects of polyphenols显示文摘 | Andriantsitohaina R Auger C Thierry Chataigneau T ltienne-Selloum N Huige Li H Marttnez MC Schini-Kerth VB Laher I | | 0，，： | 1 |
| 15 | Interfacial polymerized polyaniline/graphite oxide nanocomposites toward electrochemical energy storage显示文摘 | Jiahua Zhu Minjiao Chen Honglin Qu Xi Zhang Huige Wei Zhiping Luo Henry A. Colorado Suying Wei Zhanhu Guo | 2012 | Polymer2012，，25： | 1 |
| 16 | Process in beer oxidation control显示文摘 | Huige N J | 1993 | ACS Sympos Ser1993，536，： | 1 |
| 17 | An analysis of spatial forest structure using neighborhood-based variables显示文摘 | Aguirre O HuiG Y Gadow K v | 2003 | Forest Ecology and Management2003，183，： | 1 |
| 18 | Pseudothrombocytopenia due to agglutinins显示文摘 | Veenhoven WA van der Schans GS Huiges W | 1979 | Am J Clin Pathol1979，72，6： | 1 |
| 19 | Nitric oxide in the pathogenesis of vascular disease显示文摘 | Huige L Forstermann U | 2000 | J Pathol2000，190，3： | 1 |
| 20 | Resveratrol and stroke: from chemistry to medicine 显示文摘 | Nabavi S F Huige L Daglia M | 2014 | Curr Neurovasc Res2014，11，4： | 1 |
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