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| 1 | Characterization and complete genome sequence of vB_EcoP-Bp4,a novel polyvalent N4-like bacteriophage that infects chicken pathogenic Escherichia coli显示文摘Dear Editor,Pathogenic Escherichia coli cause chicken colibacillosis,which is economically devastating to the poultry industry worldwide(Bagheri et al.,2014).Owing to increasing antibiotic resistance,phage therapy reagents have been developed to treat bacterial infections(Xu et al.,2015).Coliphage N4 is the first reported phage in the'N4-like viruses'genus and the only member recognized | Can Zhang Yanxiang Ma Ting Wang Huzhi Sun Guomin Lu Huiying Ren | 2016 | Virologica Sinica2016，31，4： | 7 |
| 2 | Microwave–hydrothermal synthesis of fluorescent carbon dots from graphite oxide显示文摘 | Qinlong Wang Huzhi Zheng Yijuan Long Lingyan Zhang Mei Gao Wenjun Bai | 2011 | Carbon2011，，9： | 2 |
| 3 | Microwave–hydrothermal synthesis of fluorescent carbon dots from graphite oxide显示文摘 | Qinlong Wang Huzhi Zheng Yijuan Long Lingyan Zhang Mei Gao Wenjun Bai | 2011 | Carbon2011，，9： | 1 |
| 4 | Simple recurrent neutal network-based adaptivepred ietive control for onlinear systems显示文摘 | LI Xiang CHEN Zengqiang YUAN huzhi | 2002 | Asian Journal of Control2002，4，2： | 1 |
| 5 | Impact of temperature increase on the yield of winter wheat at low and high altitudes in semiarid northwestern China显示文摘 | Guoju Xiao Qiang Zhang Yu Li Runyuan Wang Yubi Yao Hong Zhao Huzhi Bai | 2010 | Agricultural Water Management2010，，9： | 1 |
| 6 | Impact of recent climatic change on the yield of winter wheat at low and high altitudes in semi-arid northwestern China显示文摘 | Guoju Xiao Qiang Zhang Yubi Yao Hong Zhao Runyuan Wang Huzhi Bai Fengju Zhang | 2008 | Agriculture Ecosystems and Environment2008，，1： | 1 |
| 7 | Camera calibration and 3D reconstruction from signal view based on scene constraints显示文摘 | WANGGH TSUIHT HUZHY etal | 2005 | Image and Vision Computing2005，23，： | 1 |
| 8 | A carbon dots-based fluorescence turn-on method for DNA determination 显示文摘 | Bai Wenjun Zheng Huzhi Long Yijuan | 2011 | Anal Sci2011，27，3： | 1 |
| 9 | A mitochondria-targeting lipid——small molecule hybrid nanoparticle for imaging and therapy in an orthotopic glioma model显示文摘Hybrid lipid-nanoparticle complexes have shown attractive characteristics as drug carriers due to their integrated advantages from liposomes and nanoparticles.Here we developed a kind of lipid-small molecule hybrid nanoparticles(LPHNPs) for imaging and treatment in an ortho topic glioma model.LPHNPs were prepared by engineering the co-assembly of lipids and an amphiphilic pheophorbide a-quinolinium conjugate(PQC),a mitochondria-targeting small molecule.Compared with the pure nanofiber self-assembled by PQC,LPHNPs not only preserve the comparable antiproliferative potency,but also possess a spherical nanostructure that allows the PQC molecules to be administrated through intravenous injection.Also,this co-assembly remarkably improved the drug-loading capacity and formulation stability against the physical encapsulation using conventional liposomes.By integrating the advantages from liposome and PQC molecule,LPHNPs have minimal system toxicity,enhanced potency of photodynamic therapy(PDT) and visualization capacities of drug biodistribution and tumor imaging.The hybrid nanoparticle demonstrates excellent curative effects to significantly prolong the survival of mice with the orthotopic glioma.The unique co-assembly of lipid and small molecule provides new potential for constructing new liposome-derived nanoformulations and improving cancer treatment. | Menghuan Tang Kai Lin Mythili Ramachandran Longmeng Li Hongye Zou Huzhi Zheng Zhao Ma Yuanpei Li | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 1 |
| 10 | Positive charged polymer as a probe for DNA determination by resonance light scattering显示文摘 | DU Juan ZHENG Huzhi HUANG Chengzhi | 2009 | Analytical Sciences2009，25，5： | 1 |
| 11 | LncRNA LOC103694972 promotes fibrosis of NRK-49F cells by regulating STAT3-dependent Smad/CTGF pathway via targeting miR-29c-3p显示文摘Background:Renalfibrosis is an important process in the development of chronic kidney disease.Understanding the pathogenesis andfinding effective treatments for renalfibrosis is crucial.This study aims to investigate whether a newly discovered long non-coding RNA(lncRNA)called LOC103694972 could be a potential target for treatingfibrosis of NRK-49F cells.Methods:LncRNA Chip was used to identify differentially expressed lncRNAs between TGF-β1-induced NRK-49F cells and normal cells.The dual-luciferase assay confirmed the binding between miR-29c-3p and signal transducer and activator of transcription(STAT3),as well as between miR-29c-3p and lncRNA LOC103694972.Si-LOC103694972 and miR-29c-3p mimic were then transfected into TGF-β1-induced NRK-49F cells.Results:The study found that LOC103694972 was highly expressed in TGF-β1-induced NRK-49F cells.These cells exhibited increased cell length and activity compared to the control group.The expression levels of Collagen I,α-Smooth muscle actin(α-SMA),and tissue inhibitor of metalloproteinase(TIMP-1)were increased,while matrix Metalloproteinase 2(MMP2)and matrix Metalloproteinase 9(MMP9)expression was decreased.However,transfection with si-LOC103694972 and miR-29c-3p mimics restored cell morphology and reduced cell viability.This led to a decrease in the levels of Collagen I,α-SMA,and TIMP-1,as well as an increase in MMP2 and MMP9 expression.Additionally,TGF-β1-induced NRK-49F cells transfected with miR-29c-3p mimics activated the STAT3-Smad3/CTGF pathway.Conclusion:Based on thesefindings,lncRNA LOC103694972 shows promise as a target for treating renalfibrosis.It negatively regulates miR-29c-3p and activates the STAT3-Smad3/CTGF pathway. | YAN LI HUZHI CAI XIAOLING PENG YOUHUI LIU QINGYANG CHEN XIANGDONG LIN XINYU CHEN | 2024 | BIOCELL2024，48，3： | 0 |
| 12 | ATP Mimics pH-Dependent Dual Peroxidase-Catalase Activities Driving H_(2)O_(2) Decomposition显示文摘Adenosine triphosphate(ATP)is produced mainly in the mitochondrion,and its primary task is to function as a ubiquitous energy currency to meet the cellular metabolic demands in biological systems.Thus far,its potential role as performing enzymatic functions has not been elucidated. | Ying Shi Menghuan Tang Chaoqun Sun Yadi Pan Li Liu Yijuan Long Huzhi Zheng | 2019 | CCS Chemistry2019，1，4： | 0 |
| 13 | Multiple-site mutations of phage Bp7 endolysin improves its activities against target bacteria显示文摘The widespread use of antibiotics has caused serious drug resistance. Bacteria that were once easily treatable are now extremely difficult to treat. Endolysin can be used as an alternative to antibiotics for the treatment of drug-resistant bacteria. To analyze the antibacterial activity of the endolysin of phage Bp7(Bp7e), a 489-bp DNA fragment of endolysin Bp7e was PCR-amplified from a phage Bp7 genome and cloned, and then a p ET28a-Bp7e prokaryotic expression vector was constructed. Two amino acids were mutated(L99A, M102E) to construct p ET28a-Bp7Δe, with p ET28a-Bp7e as a template. Phylogenetic analysis suggested that BP7e belongs to a T4-like phage endolysin group. Bp7e and its mutant Bp7Δe were expressed in Escherichia coli BL21(DE3) as soluble proteins. They were purified by affinity chromatography, and then their antibacterial activities were analyzed. The results demonstrated that the recombinant proteins Bp7e and Bp7Δe showed obvious antibacterial activity against Micrococcus lysodeikticus but no activity against Staphylococcus aureus. In the presence of malic acid, Bp7e and Bp7Δe exhibited an effect on most E. coli strains which could be lysed by phage Bp7, but no effect on Salmonella paratyphi or Pseudomonas aeruginosa. Moreover, Bp7Δe with double-site mutations showed stronger antibacterial activity and a broader lysis range than Bp7e. | Can Zhang Yuanchao Wang Huzhi Sun Huiying Ren | 2015 | Virologica Sinica2015，30，5： | 0 |
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