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| 1 | Quercetin induced HepG2 cells apoptosis through ATM/JNK/STAT3 signaling pathways显示文摘Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of death due to cancer.Quercetin,a flavonoid with low toxicity,widely exists in various fruits and vegetables.It has the potential to be a therapeutic agent against various cancers.This study aimed to demonstrate the anti-tumor effect of quercetin on HepG2 cells.Quercetin suppressed the HepG2 cell proliferation in a dose-dependent manner in cell viability assay.Induction of cell apoptosis was confirmed by apoptotic cells population(sub-G1 peak)detected by flow cytometer.A decrease in mitochondrial membrane potential and caspase-3 activation were also demonstrated in this study.Furthermore,quercetin induced HepG2 cell apoptosis through ROS-mediated phosphorylated ataxia-telangiectasia mutated,c-Jun Nterminal kinases,signal transducer,and activator of transcription 3(STAT-3),and Bax signaling pathways.These results suggest that quercetin has the potential to become an effective drug against the tumor. | WANTONG LIU DANYANG CHEN JINGYAO SU RUILIN ZHENG RAN KONG BING ZHU HAO DONG YINGHUA LI | 2023 | BIOCELL2023，47，1： | 0 |
| 2 | Halide Anchors for Single-Cluster Electronics显示文摘Due to their unique electronic structure,well-defined metal clusters at the atomic level are promising materials for single-cluster electronics.However,coupling between the electrode and the cluster remains challenging mainly due to the coverage of bulky ligands on the noble clusters.Using the scanning tunneling microscopy break junction(STM-BJ)technique,we have developed a“direct contact”approach to fabrication and investigation of the charge transport through single-cluster junctions of AgCu bimetallic metal clusterswith different halide anchors.Wefound that the electrodes could make contact directly with the surface halides of the single-cluster junctions and experience different contact resistance from different halogen atoms.Experiments and calculations reveal that the halide anchors provided efficient coupling between the cluster and the electrode,and the enhanced coupling with various halide anchors promoted electron transport and improved transmission probability.Our work offers a“direct contact”strategy for interface design between clusters of noble metals and electrodes,an essential step in progress toward single-cluster electronics. | Caiyun Wei Jingyao Ye Yuming Su Jueting Zheng Siqiang Xiao Jiawei Chen Saisai Yuan Chengyang Zhang Jie Bai Han Xu Jia Shi Jiale Huang Wenjing Hong | 2023 | CCS Chemistry2023，5，7： | 0 |
| 3 | L-Selenocystine induce HepG2 cells apoptosis through ROS-mediated signaling pathways显示文摘At present,Hepatocarcinoma is one of the main causes of tumor related death all over the world.However,there are still many clinical restrictions on the treatment of liver cancer.Recently,L-Selenocystine has been shown to be a novel treatment for tumors,especially human glioma cells.But,the mechanism of L-Selenocystine against hepatocellular carcinoma remains unclear.Therefore,the main objective of this study was to investigate the effects of L-Selenocystine on HepG2 cell proliferation and activation of reactive oxygen species(ROS)mediated signaling pathway.L-Selenocystine can significantly inhibit HepG2 cell proliferation by activating caspase-3 and cleaving PARP to induce apoptosis.Moreover,the excessive production of ROS and the influence of Bax signaling pathway which can promote cell apoptosis are key factors for L-Selenocystine to induce HepG2 cell apoptosis.Therefore,the date of this study suggest that ROS mediated signal transduction mechanism may provide certain reference significance for L-Selenocystine induced HepG2 cell apoptosis. | HAIYANG CHEN JINGYAO SU DANYANG CHEN YUYE DU RUILIN ZHENG QINGLIN DENG QIANQIAN DU BING ZHU YINGHUA LI | 2022 | BIOCELL2022，46，10： | 0 |
| 4 | Inhibition of H_(2)O_(2)-induced apoptosis of GC2-spg cells by functionalized selenium nanoparticles with lentinan through ROSmediated ERK/p53 signaling pathways显示文摘AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress. | MIAOMIAO LI DANYANG CHEN JUNYI KE RUILIN ZHENG JINGYAO SU ZILIN ZHENG JIEYI LUO HANRAN MAI FAN JIANG YANXIA QU XIAOQIONG GU BING ZHU YINGHUA LI LIANDONG ZUO | 2023 | BIOCELL2023，47，2： | 0 |
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