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| 1 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 2 | GGDEF and EAL proteins play different roles in the control of Sinorhizobium melUoti growth, motility, exopolysaccharide production, and competitive nodulation on host alfalfa显示文摘一个新细菌的第二等的送信人,二度 --(3,5 ) 周期的二聚的 guanosine monophosphate (c-di-GMP ) ,通常被综合或由包含的蛋白质分解了 GGDEF 或 glutamatealanineleucine (EAL ) 领域。因为 c-di-GMP 和他们的基因的 cyclase 或 phosphodiesterase 根据已知的 genomic DNA 序列在几乎所有细菌之中是分布式的,他们经常行动。然而, GGDEF 和 EAL 基因的系统的鉴定在 rhizobia 仍然保持不清楚,与兼容的荚交往到的土壤细菌形成修理氮的小瘤。在这研究, 19 通常认为的 GGDEF 和 EAL 基因在模型根瘤菌被识别, Sinorhizobium meliloti,由生物信息的分析(编码 5 GGDEF 蛋白质和 EAL 双域蛋白质) 。14 基因的空异种通过系统的 plasmid 插入被构造。所有 14 基因异种在最小的中等、有缺点的活动性显示出缺乏的生长,并且 11 基因异种生产了更多的 exopolysaccharide 并且在主人工厂上显示了更少的竞争生节,苜蓿。我们的结果建议 GGDEF 和 EAL 蛋白质可以在 S 的控制起不同作用。meliloti 生理学,尽管他们包含保存催化(GGDEF 或 EAL ) 领域。我们发现也暗示了那 c-di-GMP 可以在在这根瘤菌和它的主人之间的相互作用起一个重要作用种建立有效共生。 | Yiwen Wang Ji Xu Aimin Chen Yanzhang Wang Jiabi Zhu Guanqiao Yu Ling Xu Li Luo | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 5 |
| 3 | Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA mutant background显示文摘Gene expression profiles of a Si- norhizobium meliloti 1021 nifA mutant and wild type nodule bacteria were compared using whole genome microarrays. The results revealed a large scale al- teration of gene expression (601 genes) in the nifA minus background. The loss of NifA altered the ex- pression of many functional groups of genes (mac- romolecular metabolism, TCA cycle and respiration, nodulation and nitrogen fixation) and may lead to quite different life stages of the nodule bacteria. Upregulation of fixK and its associated genes was observed in the nifA mutant nodule bacteria. Addi- tional quantitative real-time PCR experiments re- vealed that the transcript levels of fixLJ were signifi- cantly upshifted in the nifA mutant nodule bacteria. Putative NifA binding sites were predicted by a statis- tical method in the upstream sequences of 13 differ- entially regulated genes from the nifA- transcriptome. | TIAN Zhexian ZOU Huasong LI Jian ZHANG Yuantao LIU Ying YU Guanqiao ZHU Jiabi RUBERG Silvia BECKER Anke WANG Yiping | 2006 | Chinese Science Bulletin2006，51，17： | 4 |
| 4 | The enoyl-ACP reductase gene, fabI1, of Sinorhizobium meliloti is involved in salt tolerance, swarming mobility and nodulation efficiency显示文摘Our previous work showed that an enoyl-ACP reductase gene fabI1 of Sinorhizobium meliloti was down-regulated in the nifA mutant nodule bacteria. To gain a better understanding of fabI1 gene, a single site insertion mutant was constructed in this study. The fabI1 mutant was retarded in cell growth, and its ability to grow on media with high concentration of NaCl was reduced. In addition, the mutant was completely defective in swarming phenotype. During symbiosis, the fabI1 mutant had delayed nodule formation on host plants. Despite the fact that FabI1 protein showed 66% identity with another enoyl-ACP reductase FabI2 in S. meliloti, defects in fabI1 were not rescued by the plasmidborne version of fabI2. This indicated the different functions of the two FabI proteins in S. meliloti. | LIU Ying ZHU JiaBi YU GuanQiao ZOU HuaSong | 2010 | Chinese Science Bulletin2010，55，3： | 3 |
| 5 | Sinorhizobium meliloti nifA gene exerts a pleiotropic effect on nodulation through the enhanced plant defense response显示文摘Sinorhizobium meliloti nifA gene is required for the expression of a bunch of nif and fix genes. Here, we report its pleiotropic effects on the nodule formation. Compared with wild type strain, nifA mutant sig- nificantly reduced nodule suppression rate in split-root system. The plants inoculated with mutant strain produced lower amount of daidzein and less necrotic cells on their roots. In addition, the defense genes failed to be evoked by nifA mutant at the early nodulation stage. These findings indicated that host defense response was one of the mechanisms mediated by nifA gene to regulate nodule formation during symbiosis. Even though nifA mutant could increase the number of nodules in host plant, it synthesized lower Nod factors than wild type. This suggested that nifA gene mediated multiple and diverse instances in nodulation formation. | CHEN XiaoTao ZOU HuaSong YAO ZhenHua CHENG HaiPing DAI XiaoMi ZHU JiaBi YU GuanQiao | 2007 | Chinese Science Bulletin2007，52，21： | 2 |
| 6 | Extra-copy nifA enhances the nodulation efficiency of Sinorhizobium fredii显示文摘Previous investigations have shown that nifA gene is involved in nodulation and symbiotic nitrogen fixation regulation of Rhizobium. We study the role of nifA on nodulation of leguminous plants. We found that Sinorhizo-bium fredii harboring multi-copy plasmid carrying the con-stitutively expressed Klebsiella pneumoniae nifA exhibited an increase of nodulation activity and nodulation competitiveness on soybean plants. The Nod-factor secreted by the rhizobia cells containing the multi-copied nifA was assayed, and preliminary results showed that S. fredii containing the multi-copy plasmid carrying nifA produced higher strength of Nod-factor than the rhizobia containing the same plasmid carrying the vector did. | Zhao Jieping Dai Xiaomi Xu Ling Zhu Jiabi Shen Shanjiong Yu Guanqiao | 2002 | Chinese Science Bulletin2002，47，7： | 2 |
| 7 | Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes显示文摘推断的一个以前的工作 Enterobacter 下水道的 nifA 基因没恢复 Sinorhizobium meliloti nifA 的共生显型变异。在现在的学习, Bra-dyrhizobium 的 twonifA 基因日本嗯并且 Mesorhizobium huakuii 也没恢复 S 的共生显型。meliloti nifA 异种。在微数组试验的整个 genomic, 238 基因被发现是在 S 以后表示的差别。meliloti nifA 组成地在它的 nifAmutant 被表示了。相反,当表示 B.japonium 时,仅仅 20, 7 和 9 基因改变了他们的 transcriptional 层次, M。在 Sm nifA 异种的 huakuii 和 Enterobacter 下水道 nifA 基因独立。这些基因包括房子保留,精力和中央中间代谢被分类进几官能基,运输系统和共生。有趣地, nifHoperons,显示出的高表示面对任何一个 B 铺平的基因。japonium 或 M。huakuii NifA,它被随后的紫胶 Z 熔化实验证实。 | YAO Zhenhua TIAN Zhexian DAI Xiaomi BECKER Anke LI Jian YAN Haiqin XIAO Yan ZHU Jiabi YU Guanqiao RUVERG Silvia WANG Yiping ZOU Huasong | 2006 | Chinese Science Bulletin2006，51，22： | 2 |
| 8 | Functional difference between Sinorhizobium meliloti NifA and Enterobacter cloacae NifA显示文摘The nifA gene is an important regulatory gene and its product, NifA protein, regulates the expression of many nif genes involved in the nitrogen fixation process. We introduced multiple copies of the constitutively expressed Sinorhizobium meliloti (Sm) or Enterobacter cloacae (Ec) nifA gene into both the nifA mutant strain SmY and the wild-type strain Sm1021. Root nodules produced by SmY containing a constitutively expressed Sm nifA gene were capable of fixing nitrogen, while nodules produced by SmY containing the Ec nifA gene remained unable to fix nitrogen, as is the case for SmY itself. However, transfer of an additional Sm nifA gene into Sm1021 improved the nitrogen-fixing efficiency of root nodules to a greater extent than that observed upon transfer of the Ec nifA gene into Sm1021. Comparative analysis of amino acid sequences between Sm NifA and Ec NifA showed that the N-terminal domain was the least similar, but this domain is indispensable for complementation of the Fix? phenotype of SmY by Sm NifA. We conclude that more than one domain is involved in determining functional differences between Sm NifA and Ec NifA. | YANG Chengtao YU Guanqiao SHEN Shanjiong(San Chiun Shen) ZHU Jiabi | 2004 | Science China(Life Sciences)2004，47，1： | 2 |
| 9 | Acoustic performance prediction of Helmholtz resonator with conical neck显示文摘There is no accurate analytical approach for the acoustic performance prediction of Helmholtz resonator with conical neck,which has broad band acoustic attenuation performance in the low frequency range.To predict the acoustic performance of the resonator accurately,a general theory model based on the one-dimensional analysis approach with acoustic length corrections is developed.The segmentation method is used to calculate the acoustic parameters for sound propagation in conical tubes.And then,an approximate formula is deduced to give accurate correction lengths for conical tubes with difierent geometries.The deviations of the resonance frequency between the transmission loss results obtained by the general theory with acoustic lengths correction and the results from the finite element method and experiments are less than 2 Hz,which is much better than the results from one-dimensional approach without corrections.The results show that the method of acoustic length correction for the conical neck greatly improved the accuracy of the one-dimensional analysis approach,and it will be quick and accurate to predict the sound attenuation property of Helmholtz resonator with conical neck. | LIU Haitao ZHENG Sifa LIAN Xiaomin DAN Jiabi | 2014 | Chinese Journal of Acoustics2014，33，4： | 2 |
| 10 | Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti显示文摘The 660 bp region between nodD3 P1 promoter and the following coding region of Rnizopium meliloti has been studied. This region is designated 'downstream sequences' . it consists of two potential open reading frames, ORF1 and ORF2. Studies on the role of the downstream sequences on the activity of nooD3 P1 with nod D3(P1)-/acZ fusion show that deletion of the seguences containing ORF2 causes the increase of the activity of the fusion; on the contrary, addition of extra copies of ORF2 markedly decreases the activity of the fusion. These results indicate that the product of ORF2 plays a negative role in the expression of nod D3. | Bing Zhu Xiaomi Dai Jiabi Zhu Guanqiao Yu Shanjiong Shen | 2000 | Chinese Science Bulletin2000，45，1： | 2 |
| 11 | Long-period fiber grating thin film sensors based on cladding mode coupling显示文摘 | Xu Yanping Gu Zhengtian Chen Jiabi | 2005 | Chin Phys Lett2005，22，7： | 1 |
| 12 | High performance liquid chromatography electrospray ionization mass spec trometry determination of mitiglinide in human plasma and its pharmacokinetics显示文摘 | Jiabi Liang Yuan Tian Zunjian Zhang | 2006 | Journal of Mass Spectrom2006，41，： | 1 |
| 13 | Systematic insertion mutagenesis of GntR family tran-scriptional regulator genes in Sinorhizobium meliloti显示文摘GntR-type transcriptional regulators regulate the most diverse biological processes in bacteria. Al- though GntR-type transcriptional regulators consist of the second largest family of transcriptional regulators in Sinorhizobium meliloti, little is known about their functions. In this study, we investigated 54 putative genes encoding GntR family of transcriptional regulators in S. meliloti Rm1021. Secondary structure analysis of the C-terminal domain of these putative transcriptional regulators indicated that thirty-seven were members of the FadR subfamily, ten of the HutC subfamily and five of the MocR subfamily. The remaining two did not fall into any specific subfamily category, and may form two new subfamilies. The 54 gntR genes were mutagenized by plasmid insertion mutagenesis to investigate their roles. We found that, of the 54 mutants, only the gtrA1 and gtrB1 mutants had slower growth rates and cell maximal yields on both rich medium and minimal medium, and lower cell motility on swarming plate than wild type Rm1021. All mutants, with the exception of gtrA1 and gtrB1, can establish effective symbioses with alfalfa. Plants inoculated with gtrA1 and gtrB1 mutants grew shorter than those in- oculated with wild type, and formed relatively smaller, round and light pink nodules, which were mainly located on lateral roots. And there was an abnormal increase in the number of nodules induced by both mutants. These results suggested that the gtrA1 and gtrB1 mutants were symbiotically deficient. Our work presents a global overview of GntR-like transcriptional regulators involved in symbiosis in S. meliloti, and provides new insight into the functions of GntR-like transcriptional regulators. | YU AiYuan CHEN AiMin WANG Yi LUO Li ZHU JiaBi YU GuanQiao WANG YanZhang | 2008 | Chinese Science Bulletin2008，53，2： | 1 |
| 14 | Effects on seeds of Ziziphus spinosa Huon immune function mice显示文摘 | LangXC LiMX JiaBY | 1988 | Bull Chin Mater Med1988，13，： | 1 |
| 15 | Opposite Regulation of Chondrogenesis and Angiogenesis in Cartilage Repair ECM Materials under Hypoxia显示文摘Although cartilage tissue engineering has been developed for decades, it is still unclear whether angiogenesis was the accompaniment of chondrogenesis in cartilage regeneration. This study aimed to explore the process of anti-angiogenesis during cartilage regenerative progress in cartilage repair extracellular matrix(ECM) materials under Hypoxia. C3H10T1/2 cell line, seeded as pellet or in ECM materials, was added with chondrogenic medium or DMEM medium for 21 days under hypoxia or normoxia environment. Genes and mi RNAs related with chondrogenesis and angiogenesis were detected by RT-q PCR technique on Days 7, 14, and 21. Dual-luciferase report system was used to explore the regulating roles of mi RNAs on angiogenesis. Results showed that the chondrogenic medium promotes chondrogenesis both in pellet and ECM materials culture. HIF1α was up-regulated under hypoxia compared with normoxia(P < 0.05). Meanwhile, hypoxia enhanced chondrogenesis. miR-140-5p exhibited higher expression while miR-146 b exhibited lower expression. The chondrogenic phenotype was more stabilized in the ECM materials in chondrogenic medium than DMEM medium, with lower VEGFα expression even under hypoxia.Dual-luciferase report assays demonstrated that mi R-140-5p directly targets VEGFα by binding its 3′-UTR. Taken together, chondrogenic cytokines, ECM materials and hypoxia synergistically promoted chondrogenesis and inhibited angiogenesis. mi R-140-5p played an important role in this process. | Shaoming Chen Manman Gao Zhiyu Zhou Jiabi Liang Ming Gong Xuejun Dai Tangzhao Liang Jiacheng Ye Gang Wu Lijin Zou Yingjun Wang Xuenong Zou | 2016 | Journal of Materials Science & Technology2016，32，9： | 1 |
| 16 | Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa显示文摘热的一些成员震惊蛋白质 70 (HSP70 ) 家庭在有机体开发有重要功能。这里,我们识别了一棵苜蓿(Medicago sativa L。cv。阿尔冈京族) HSP70 基因, MsHSP70-1,使用的 cDNA 数组和反向的抄写聚合酶锁住反应。这基因包含 1947 bp 编码 649 氨基酸的蛋白质的开的读框架。这蛋白质包含植物 HSP70s 的所有保存领域,主题,和典型序列。MsHSP70-1 的表达式与根,茎,叶,和花相比在小瘤被提高,并且在整个小瘤开发的进程。北杂交分析显示在小瘤的 MsHSP70-1 的表示要求 rhizobia 的活跃用绳子拖的平底渡船 A 基因。这些结果建议 MsHSP70-1 可能在苜蓿小瘤开发起一个重要作用。 | Zhishui He Rong Xie Yanzhang Wang Huasong Zou Jiabi Zhu Guanqiao Yu | 2008 | Acta Biochimica et Biophysica Sinica2008，40，3： | 1 |
| 17 | Treating COVID-19 with Chloroquine显示文摘A novel coronavirus disease 2019(COVID-19)emerged around December 2019 in Wuhan,China and has spread rapidly worldwide(Lu et al.,2020).Until March 27,2020,the Chinese health authorities had reported 82082 confirmed COVID-19 cases in China with 3298 deaths and 381443 confirmed cases with 20787 deaths outside China.The World Health Organization(WHO)named the virus SARS-CoV-2,which belongs to a distinct clade from the human severe acute respiratory syndrome CoV(SARS-CoV)and Middle East respiratory syndrome CoV(MERSCoV)(Zhu et al.,2020). | Mingxing Huang Tiantian Tang Pengfei Pang Man Li Ruolan Ma Jiahui Lu Jingxian Shu Yingying You Binghui Chen Jiabi Liang Zhongsi Hong Huili Chen Ling Kong Dajiang Qin Duanqing Pei Jinyu Xia Shanping jiang Hong Shan | 2020 | Journal of Molecular Cell Biology2020，12，4： | 1 |
| 18 | A nontoxic disulfide bond reducing method for lipophilic drug-loaded albumin nanoparticle preparation: Formation dynamics, influencing factors and formation mechanisms investigation显示文摘 | Liqun Jiang Yisheng Xu Qi Liu Yue Tang Liang Ge Chunli Zheng Jiabi Zhu Jianping Liu | 2013 | International Journal of Pharmaceutics (-)2013，，1： | 1 |
| 19 | Identification of genes induced during Medicago sativa nodule development by using the cDNA-AFLP technique显示文摘A variety of genes involved in nodule development and function were induced during sym biotic root nodule development in alfalfa. We identi fied those genes by comparing the cDNA-amplified fragment length polymorphism (cDNA-AFLP) pat terns of infected nodules and uninfected roots. Sev enty-one transcript-derived fragments (TDFs) were isolated from about 3000 TDFs, which are enhanced or specifically expressed in nodule. The differentia expression patterns of 61 genes were confirmed b reverse Northern hybridization or Northern hybridiza tion analyses. Among these, 31 exhibited significan similarities to characterized database entries. These results suggest that the genes corresponding to the 31 TDFs may play important roles in signal transduc tion, gene expression and regulation, substance transportation or auto-regulation of nodulation during nodule development. The Northern hybridization analysis also indicates that the gene corresponding to TDF RX89 was not expressed in the nodule elic ited by bacA mutant, presumably it is specially in duced at the stage of nodule development when bacteroids begin to be differentiated. | XIE Rong HE Zhishui LIU Fanghua ZOU Huasong ZHU Jiabi YU Guanqiao | 2006 | Chinese Science Bulletin2006，51，17： | 1 |
| 20 | Achieving integrated capture and reduction of CO_(2):A promising electrocatalyst显示文摘To realize the goal of carbon peaking and carbon neutrality,CO_(2) capture and utilization technology is becoming increasingly urgent.However,the low efficiency and complex processes limit its large-scale application.Among utilization technology of CO_(2),electrochemical CO_(2) reduction reaction(CO_(2)RR)has attracted interest,attributing to the use of clean energy and the final product of value-added fuels.The construction of catalysts with integrated CO_(2) capture and reduction ability is expected to bridge the gap between CO_(2) capture and conversion,achieving the direct utilization of flue gas,reducing costs,and simplifying devices.Keeping` this in mind,we give this review to introduce the problems of lowconcentration CO_(2)RR and the significance of coupled CO_(2) capture/conversion electrocatalysts in addressing the emission of industrial flue gas first.Then,the regulation strategies,including functional group modification,co-catalytic effect,and catalysts-impurities interaction,which affected the CO_(2) capture and electroreduction capacity towards catalysts were summarized and discussed.Finally,the challenges and perspectives about the design for linked CO_(2) capture/conversion processes were proposed.This review will provide new insight into the building of multifunctional catalysts for CO_(2) conversion. | Mengjie Liu Longsheng Zhan Yuchao Wang Xin Zhao Jiao Wu Danni Deng Jiabi Jiang Xinran Zheng Yongpeng Lei | 2023 | Journal of Materials Science & Technology2023，，34： | 0 |
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