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| 1 | Cold-chain transportation in the frozen food industry may have caused a recurrence of COVID-19 cases in destination:Successful isolation of SARS-CoV-2 virus from the imported frozen cod package surface显示文摘Coronavirus disease 2019(COVID-19)pandemic has spread in 220 countries/regions towreak havoc to human beings around theworld.At present,the second wave of COVID-19 has begun in many European countries.The complete control of COVID-19 is very urgent.Although China quickly brought the virus under control,there have been eight sporadic outbreaks in China since then.Both in Xinfadi of Beijing and Dalian outbreak of COVID-19,environmental swab samples related to imported cold chain food were tested nucleic acid positive for SARS-CoV-2.In this outbreak in Qingdao,we directly isolated SARS-CoV-2 from the cod outer package's surface swab samples.This is the first time worldwide,SARS-CoV-2 were isolated from the imported frozen cod outer package's surface,which showed that imported frozen food industry could import SARS-CoV-2 virus. | Peipei Liu Mengjie Yang Xiang Zhao Yuanyuan Guo Liang Wang Jing Zhang Wenwen Lei Weifang Han Fachun Jiang William J.Liu George F.Gao Guizhen Wu | 2020 | Biosafety and Health2020，2，4： | 41 |
| 2 | Epidemiological Characteristics of Newly-Reported HIV Cases Among Youth Aged 15−24 Years-China, 2010−2019显示文摘Summary What is already known about this topic?An estimated 1,400 new cases of human immunodeficiency virus(HIV)occur every day among youths globally.However,HIV distribution among youth aged 15–24 years in China has not been researched extensively. | Hao Zhao Hui Liu Lu Wang Xuan Yang Shaorong Wang Mengjie Han Jian Li | 2020 | China CDC weekly2020，2，48： | 23 |
| 3 | Exploring the molecular basis of heterosis for plant breeding显示文摘Since approximate a century ago, many hybrid crops have been continually developed by crossing two inbred varieties. Owing to heterosis(hybrid vigor) in plants, these hybrids often have superior agricultural performances in yield or disease resistance succeeding their inbred parental lines. Several classical hypotheses have been proposed to explain the genetic causes of heterosis. During recent years, many new genetics and genomics strategies have been developed and used for the identifications of heterotic genes in plants. Heterotic effects of the heterotic loci and molecular functions of the heterotic genes are being investigated in many plants such as rice, maize, sorghum, Arabidopsis and tomato.More and more data and knowledge coming from the molecular studies of heterotic loci and genes will serve as a valuable resource for hybrid breeding by molecular design in future. This review aims to address recent advances in our understanding of the genetic and molecular mechanisms of heterosis in plants. The remaining scientific questions on the molecular basis of heterosis and the potential applications in breeding are also proposed and discussed. | Jie Liu Mengjie Li Qi Zhang Xin Wei Xuehui Huang | 2020 | Journal of Integrative Plant Biology2020，62，3： | 13 |
| 4 | New therapeutic vaccination strategies for the treatment of chronic hepatitis B显示文摘Chronic hepatitis B virus(CHB) is currently treated with either interferon-based or nucleot(s)idebased antiviral therapies.However,treatment with pegylated interferon alpha results in a durable antiviral response in only about 30%patients and is associated with side effects.Most patients receiving nucleot(s)ide analogue treatment do not establish long-term,durable control of Infection and have rebounding viremia after cessation of therapy.Thus,novel therapy strategies are necessary to achieve the induction of potent and durable antiviral immune responses of the patients which can maintain long-term control of viral replication.Therapeutic vaccination of HBV carriers is a promising strategy for the control of hepatitis B.Here the authors review new therapeutic vaccination strategies to treat chronic hepatitis B which may be introduced for patient treatment in the future. | Jia Liu Anna Kosinska Mengji Lu Michael Roggendorf | 2014 | Virologica Sinica2014，29，1： | 9 |
| 5 | Cryo-EM structure of human mTOR complex 2显示文摘 | Xizi Chen Mengjie Liu Yuan Tian Jiabei Li Yilun Qi Dan Zhao Zihan Wu Min Huang Catherine C. L. Wong Hong-Wei Wang Jiawei Wang Huirong Yang Yanhui Xu | 2018 | Cell Research2018，28，5： | 8 |
| 6 | Stepwise-Nanocavity-Assisted Transmissive Color Filter Array Microprints显示文摘Visible-light color flters using patterned nanostructures have attracted much interest due to their various advantages such as compactness,enhanced stability,and environmental friendliness compared with traditional pigment or dye-based optical flters.While most existing studies are based on planar nanostructures with lateral variation in size,shape,and arrangement,the vertical dimension of structures is a long-ignored degree of freedom for the structural colors.Herein,we demonstrate a synthetic platform for transmissive color flter array by coordinated manipulations between height-varying nanocavities and their lateral flling fractions.Te thickness variation of those nanocavities has been fully deployed as an alternative degree of freedom,yielding vivid colors with wide gamut and excellent saturation.Experimental results show that the color-rendering capability of the pixelated nanocavities can be still retained as pixels are miniaturized to 500 nm.Crosstalk between closely spaced pixels of a Bayer color flter arrangement was calculated,showing minimal crosstalk for 1μm2 square subpixels.Our work provides an approach to designing and fabricating ultracompact color flter arrays for various potential applications including stained-glass microprints,microspectrometers,and high-resolution image sensing systems. | Yasi Wang Mengjie Zheng Qifeng Ruan Yanming Zhou Yiqin Chen Peng Dai Zhengmei Yang Zihao Lin Yuxiang Long Ying Li Na Liu Cheng-Wei Qiu Joel K.W.Yang Huigao Duan | 2018 | Research2018，，1： | 8 |
| 7 | Cryo-EM structure of human DNA-PK holoenzyme显示文摘 | Xiaotong Yin Mengjie Liu Yuan Tian Jiawei Wang Yanhui Xu | 2017 | Cell Research2017，27，11： | 8 |
| 8 | Ultraselective carbon molecular sieve membrane for hydrogen purification显示文摘Hydrogen is a green clean fuel and chemical feedstock. Its separation and purification from hydrogencontaining mixtures is the key step in the production of hydrogen with high purity(>99.99%). In this work, carbon molecular sieve(CMS) membranes with ultrahigh permselectivity for hydrogen purification were fabricated by high-temperature(700–900 ℃) pyrolysis of polymeric precursor of phenolphthaleinbased cardo poly(arylene ether ketone)(PEK-C). The evolution of the microstructural texture and ultramicroporous structure and gas separation performance of the CMS membrane were characterized via TG-MS, FT-IR, XRD, TEM, CO2 sorption analysis and gas permeation measurements. CMS membranes prepared at 700 ℃ exhibited amorphous turbostratic carbon structures and high H2 permeability of 5260 Barrer with H2/CH4, H2/N2 and H2/CO selectivities of 311, 142, 75, respectively. When carbonized at900 ℃, the CMS membrane with ultrahigh H2/CH4 selectivity of 1859 was derived owing to the formation of the dense and ordered carbon structure. CMS membranes with ultrahigh permselectivity exhibit an attractive application prospect in hydrogen purification. | Ruisong Xu Liu He Lin Li Mengjie Hou Yongzhao Wang Bingsen Zhang Changhai Liang Tonghua Wang | 2020 | Journal of Energy Chemistry2020，29，11： | 8 |
| 9 | Single-cell Immune Landscape of Human Recurrent Miscarriage显示文摘t Successful pregnancy in placental mammals substantially depends on the establishment of maternal immune tolerance to the semi-allogenic fetus.Disorders in this process are tightly associated with adverse pregnancy outcomes including recurrent miscarriage(RM).However,an indepth understanding of the systematic and decidual immune environment in RM remains largely lacking.In this study,we utilized single-cell RNA-sequencing(scRNA-seq)to comparably analyze the cellular and molecular signatures of decidual and peripheral leukocytes in normal and unexplained RM pregnancies at the early stage of gestation.Integrative analysis identifies 22 distinct cell clusters in total,and a dramatic difference in leukocyte subsets and molecular properties in RM cases is revealed.Specifically,the cytotoxic properties of CD8^(+)effector T cells,nature killer(NK),and mucosal-associated invariant T(MAIT)cells in peripheral blood indicates apparently enhanced pro-inflammatory status,and the population proportions and ligand–receptor interactions of the decidual leukocyte subsets demonstrate preferential immune activation in RM patients.The molecular features,spatial distribution,and the developmental trajectories of five decidual NK(dNK)subsets have been elaborately illustrated.In RM patients,a dNK subset that supports embryonic growth is diminished in proportion,while the ratio of another dNK subset with cyto toxic and immune-active signature is significantly increased.Notably,a unique pro-inflammatory CD56^(+)CD16^(+)dNK subset substantially accumulates in RM decidua.These findings reveal a comprehensive cellular and molecular atlas of decidual and peripheral leukocytes in human early pregnancy and provide an in-depth insight into the immune pathogenesis for early pregnancy loss. | Feiyang Wang Wentong Jia Mengjie Fan Xuan Shao Zhilang Li Yongjie Liu Yeling Ma Yu-Xia Li Rong Li Qiang Tu Yan-Ling Wang | 2021 | Genomics, Proteomics & Bioinformatics2021，19，2： | 8 |
| 10 | 4.4 A Resolution Cryo-EM structure of human mTOR Complex 1显示文摘 | Huirong Yang Jia Wang Mengjie Liu Xizi Chen Min Huang Dan Tan Meng-Qiu Dong Catherine C. L, Wong Jiawei Wang Yanhui Xu Hong-Wei Wang | 2016 | Protein & Cell2016，7，12： | 7 |
| 11 | Biothiol-specific fluorescent probes with aggregation-induced emission characteristics显示文摘Biothiols are important species in physiological processes such as regulating protein structures, redox homeostasis and cell signalling. Alternation in the biothiol levels is associated with various pathological processes, therefore non-invasive fluorescent probes with high specificity to biothiols are highly desirable research utilities. Meanwhile, fluorescent probes with aggregationinduced emission properties(AIEgens) possess unique photophysical properties that allow modulation of the sensing process through controlling the aggregation-disaggregation or the intramolecular rotational motions of the fluorophores. Herein we review the recent progress in the development of biothiol-specific AIEgens. In particular, the molecular design principles to target different types of biothiols and the corresponding sensing mechanisms are discussed, along with the potential of the future design and development of multi-functional bioprobes. | Siyang Ding Mengjie Liu Yuning Hong | 2018 | Science China Chemistry2018，61，8： | 7 |
| 12 | The IL-1R/TLR signaling pathway is essential for efficient CD8+ T-cell responses against hepatitis B virus in the hydrodynamic injection mouse model显示文摘The outcome of hepatitis B viral(HBV)infection is determined by the complex interactions between replicating HBV and the immune system.While the role of the adaptive immune system in the resolution of HBV infection has been studied extensively,the contribution of innate immune mechanisms remains to be defined.Here we examined the role of the interleukin-1 receptor/Toll-like receptor(IL-1R/TLR)signaling pathway in adaptive immune responses and viral clearance by exploring the HBV mouse model.Hydrodynamic injection with a replication-competent HBV genome was performed in wild-type mice(WT)and a panel of mouse strains lacking specific innate immunity component expression.We found higher levels of HBV protein production and replication in Tlr2^(−/−),Tlr23479^(−/−),3d/Tlr24^(−/−),Myd88/Trif^(−/−)and Irak4^(−/−)mice,which was associated with reduced HBV-specific CD8+T-cell responses in these mice.Importantly,HBV clearance was delayed for more than 2 weeks in 3d/Tlr24^(−/−),Myd88/Trif^(−/−)and Irak4^(−/−)mice compared to WT mice.HBV-specific CD8+T-cell responses were functionally impaired for producing the cytokines IFN-γ,TNF-αand IL-2 in TLR signaling-deficient mice compared to WT mice.In conclusion,the IL-1R/TLR signaling pathway might contribute to controlling HBV infection by augmenting HBV-specific CD8+T-cell responses. | Zhiyong Ma Jia Liu Weimin Wu Ejuan Zhang Xiaoyong Zhang Qian Li Gennadiy Zelinskyy Jan Buer Ulf Dittmer Carsten J Kirschning Mengji Lu | 2017 | Cellular & Molecular Immunology2017，14，12： | 6 |
| 13 | Enhanced magnetic properties in chemically inhomogeneous Nd-Dy-Fe-B sintered magnets by multi-main-phase process显示文摘Homogeneous substitution of Dy for Nd in the hard magnetic 2:14:1 phase can effectively enhance coercivity to ensure the high temperature operation,however,inevitably deteriorate remanence at expense.In this work,we performed a comparative investigation of the two magnets prepared by multimain-phase(co-sintering Nd_(2)Fe_(14)B and(Nd,Dy)_(2)Fe_(14)B powders)and single-main-phase(sintering(Nd,Dy)_(2)Fe_(14)B powders)approaches.The comparative investigation reveals that at the same Dy substitution level(2.16 wt%),such chemically inhomogeneous multi-main-phase magnet possesses better roomtemperature magnetic properties as well as thermal stability than those of the single-main-phase one with homogenous Dy distribution in the matrix grains.Room-temperature magnetic properties H_(Cj)=1664 kA/m,B_(r)=1.33 T and(BH)_(max)=350.4 kJ/m^(3)for the multi-main-phase magnet are all better than those for the single-main-phase magnet with H_(Cj)=1536 kA/m,B_(r)=1.29 T and(BH)_(max)=318.4 kj/m^(3).In addition,over the temperature range from 295 to 423 K,both the temperature coefficients of coercivity and remanence for the multi-main-phase magnet are also lower than that for the single-main-phase magnet.Such superior magnetic performance is attributed to the short-range magnetic interactions inside individual 2:14:1 phase grains and the long-range magnetostatic interactions between adjacent grains with inhomogeneous Dy distribution.Our work provides a feasible approach of enhancing coercivity and retaining energy product simultaneously in the Nd-Dy-Fe-B permanent magnets. | Xiaolian Liu Mengjie Pan Pei Zhang Tianyu Ma Lizhong Zhao Lingwei Li | 2021 | Journal of Rare Earths2021，39，5： | 5 |
| 14 | A detached petal disc assay and virus-induced gene silencing facilitate the study of Botrytis cinerea resistance in rose flowers显示文摘Fresh-cut roses(Rosa hybrida)are one of the most important ornamental crops worldwide,with annual trade in the billions of dollars.Gray mold disease caused by the pathogen Botrytis cinerea is the most serious fungal threat to cut roses,causing extensive postharvest losses.In this study,we optimized a detached petal disc assay(DPDA)for artificial B.cinerea inoculation and quantification of disease symptoms in rose petals.Furthermore,as the identification of rose genes involved in B.cinerea resistance could provide useful genetic and genomic resources,we devised a virusinduced gene silencing(VIGS)procedure for the functional analysis of B.cinerea resistance genes in rose petals.We used RhPR10.1 as a reporter of silencing efficiency and found that the rose cultivar‘Samantha’showed the greatest decrease in RhPR10.1 expression among the cultivars tested.To determine whether jasmonic acid and ethylene are required for B.cinerea resistance in rose petals,we used VIGS to silence the expression of RhLOX5 and RhEIN3(encoding a jasmonic acid biosynthesis pathway protein and an ethylene regulatory protein,respectively)and found that petal susceptibility to B.cinerea was affected.Finally,a VIGS screen of B.cinerea-induced rose transcription factors demonstrated the potential benefits of this method for the high-throughput identification of gene function in B.cinerea resistance.Collectively,our data show that the combination of the DPDA and VIGS is a reliable and highthroughput method for studying B.cinerea resistance in rose. | Xiaoqian Cao Huijun Yan Xintong Liu Dandan Li Mengjie Sui Jie Wu Hongqiang Yu Zhao Zhang | 2019 | Horticulture Research2019，6，1： | 5 |
| 15 | A novel artificial nerve graft for repairing longdistance sciatic nerve defects:a self-assembling peptide nanofiber scaffold-containing poly (lactic-co-glycolic acid) conduit显示文摘In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold(SAPNS)-containing poly(lactic-co-glycolic acid)(PLGA) conduit(SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury. | Xianghai Wang Mengjie Pan Jinkun Wen Yinjuan Tang Audra D.Hamilton Yuanyuan Li Changhui Qian Zhongying Liu Wutian Wu Jiasong Guo | 2014 | Neural Regeneration Research2014，9，24： | 5 |
| 16 | Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference显示文摘Reverse transcription-polymerase chain reaction(RT-PCR)is an essential method for specific diagnosis of SARS-CoV-2 infection.Unfortunately,false negative test results are often reported.In this study,we attempted to determine the principal causes leading to false negative results of RT-PCR detection of SARS-CoV-2 RNAs in respiratory tract specimens.Multiple sputum and throat swab specimens from 161 confirmed COVID-19 patients were tested with a commercialfluorescent RT-PCR kit targeting the ORF1 ab and N regions of SARS-CoV-2 genome.The RNA level of a cellular housekeeping gene ribonuclease P/MRP subunit p30(RPP30)in these specimens was also assessed by RT-PCR.Data for a total of 1052 samples were retrospectively re-analyzed and a strong association between positive results in SARS-CoV-2 RNA tests and high level of RPP30 RNA in respiratory tract specimens was revealed.By using the ROC-AUC analysis,we identified Ct cutoff values for RPP30 RT-PCR which predicted false negative results for SARS-CoV-2 RT-PCR with high sensitivity(95.03%–95.26%)and specificity(83.72%–98.55%)for respective combination of specimen type and ampli-fication reaction.Using these Ct cutoff values,false negative results could be reliably identified.Therefore,the presence of cellular materials,likely infected host cells,are essential for correct SARS-CoV-2 RNA detection by RT-PCR in patient specimens.RPP30 could serve as an indicator for cellular content,or a surrogate indicator for specimen quality.In addition,our results demonstrated that false negativity accounted for a vast majority of contradicting results in SARS-CoV-2 RNA test by RT-PCR. | Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping Liu Tengfei He Tuantuan Li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang | 2020 | Virologica Sinica2020，35，6： | 5 |
| 17 | The histone methyltransferase EZH2 primes the early differentiation of follicular helper T cells during acute viral infection显示文摘Epigenetic modifications to histones dictate the differentiation of naïve CD4^(+) T cells into different subsets of effector T helper(TH)cells.The histone methyltransferase enhancer of zeste homolog 2(EZH2)has been implicated in the mechanism regulating the differentiation of TH1,TH2 and regulatory T(Treg)cells.However,whether and how EZH2 regulates follicular helper T(TFH)cell differentiation remain unknown.Using a mouse model of acute lymphocytic choriomeningitis virus(LCMV)infection,we observed abundant EZH2 expression and associated H3K27me3 modifications preferentially in the early committed virus-specific TFH cells compared to those in TH1 cells.Ablation of EZH2 in LCMV-specific CD4^(+) T cells leads to a selective impairment of early TFH cell fate commitment,but not late TFH differentiation or memory TFH maintenance.Mechanistically,EZH2 specifically stabilizes the chromatin accessibility of a cluster of genes that are important for TFH fate commitment,particularly B cell lymphoma 6(Bcl6),and thus directs TFH cell commitment.Therefore,we identified the chromatin-modifying enzyme EZH2 as a novel regulator of early TFH differentiation during acute viral infection. | Xiangyu Chen Guoshuai Cao Jialin Wu Xinxin Wang Zhiwei Pan Jianbao Gao Qin Tian Lifan Xu Zhirong Li Yaxing Hao Qizhao Huang Pengcheng Wang Minglu Xiao Luoyingzi Xie Shupei Tang Zhenyu Liu Li Hu Jianfang Tang Ran He Li Wang Xinyuan Zhou Yuzhang Wu Mengjie Chen Beicheng Sun Bo Zhu Jun Huang Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，3： | 4 |
| 18 | Cryo-EM structure of human ATR-ATRIP complex显示文摘ATR (变异毛细管扩张、 Rad3 相关的混乱) 蛋白质 kinase 并且(交往 ATR 蛋白质) 起锚形成建筑群并且响应复制应力和 DNA 损坏起一个关键作用。这里,我们决定了在 4.7 点 ATR 起锚复杂的人的 cryo 电子显微镜学(他们) 结构吗?分辨率并且造了 ATR 的 C 终端催化核心的一个原子模型(残余 1 521-2 644 ) 在 3.9 点?分辨率。建筑群采用空心形状,由在不同符合构造的二 ATR 单体组成。他们地图为起锚表明 14 热在扩大 S 形状重复。ATR 的 conformational 灵活性起锚允许适当地锁二 ATR 单体的 N 终点赞成 ATR 起锚复杂的形成和功能的差异。孤立的头头和尾巴尾巴各个采用假 2 褶层对称。外面的催化衣袋脸和底层存取没被禁止的元素限制。我们的研究为理解集会提供一个结构的基础 ATR 起锚,为描绘调停 ATR 的 DNA 的建筑群和一个框架修理小径。 | Qinhui Rao Mengjie Liu Yuan Tian Yuhan Hao Lei Song Zhaoyu Qin Chen Ding Hong-Wei Wang Jiawei Wang Yanhui Xu | 2018 | Cell Research2018，28，2： | 4 |
| 19 | HIV Prevention and Health Poverty Alleviation-Liangshan Prefecture,Sichuan Province,China,2017-2020显示文摘BACKGROUND Liangshan,officially the Liangshan Yi Autonomous Prefecture,is an autonomous prefecture occupying much of the southern portion of Sichuan Province.It has been a remote and deeply impoverished area and also has the largest population of ethnic Yi people in China(1).For many years,due to geographical environment conditions,traffic conditions,and other historic restrictions,the human immunodeficiency virus(HIV)epidemic in Liangshan Prefecture has become the focus of HIV prevention and control in China since the first acquired immunodeficiency syndrome(AIDS)case was reported in this area in 1995(2-3). | Zhongfu Liu Xuefeng Tang Yufen Liu Linglin Zhang Yihui Yang Yongli Zheng Weixin Liu Chunnong Jike Hong Mai Heying Fan Gang Yu Mengjie Han George F.Gao | 2021 | China CDC weekly2021，3，48： | 4 |
| 20 | Complementation of Wild-Type and Drug-Resistant Hepatitis B Virus Genomes to Maintain Viral Replication and Rescue Virion Production under Nucleos(t)ide Analogs显示文摘As the open reading frames of hepatitis B virus(HBV)genomes are overlapping,resistance mutations(MTs)in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type(WT)HBV.The question was raised how the coexistence of nucleos(t)ide analogs(NAs)resistance MTs and WT sequences affects HBV replication.In the present study,HBV genomes with frequently detected reverse transcriptase(RT)/surface truncation MTs,rtA181T/sW172^*,rtV191I/sW182^*and rtM204I/sW196^*,were phenotypically characterized alone or together with their WT counterparts in different ratios by transient transfection in the absence or presence of Nas.In the absence of Nas,RT/surface truncation MTs impaired the expression and secretion of HBV surface proteins,and had a dose-dependent negative effect on WT HBV virion secretion.However,in the presence of Nas,coexistence of MTs with WT maintained viral replication,and the presence of WT was able to rescue the production of MT HBV virions.Our findings reveal that complementation of WT and MT HBV genomes is highly effective under drug treatment. | Chunchen Wu Baolin Li Xiaoyong Zhang Kaitao Zhao Yingshan Chen Yifei Yuan Yan Liu Rongjuan Chen Dongping Xu Xinwen Chen Mengji Lu | 2019 | Virologica Sinica2019，34，4： | 4 |
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