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1Paleoproterozoic crustal evolution of the Hengshan-Wutai—Fuping region,North China Craton显示文摘An arguable point regarding the Neoarchean and Paleoproterozoic crustal evolution of the North China Craton(NCC)is whether the tectonic setting in the central belt during the mid-Paleoproterozoic(2.35-2.0 Ga)was dominated by an extensional regime or an oceanic subduction-arc regime.A review of the midPaleoproterozoic magmatism and sedimentation for the Hengshan-Wutai-Fuping region suggests that a back-arc extension regime was dominant in this region.This conclusion is consistent with the observation that the 2.35-2.0 Ga magmatism shows a typical bimodal distribution where the mafic rocks mostly have arc affinities and the acidic rocks mainly comprise highly-fractioned calc-alkaline to alkaline(or A-type)granites,and that this magmatism was coeval with development of extensional basins characteristic of transgressive sequences with volcanic interlayers such as in the Hutuo Group.Although the final amalgamation of the NCC was believed to occur at ~1.85 Ga,recent zircon U-Pb age dating for mica schist in the Wutai Group suggests a collisional event may have occurred at ~1.95 Ga.The metamorphic ages of ~1.85 Ga,obtained mostly from the high-grade rocks using the zircon U-Pb approach,most probably indicate uplifting and cooling of these high-grade terranes.This is because(i)phase modeling suggests that newly-grown zircon grains in highgrade rocks with a melt phase cannot date the age of peak pressure and temperature stages,but the age of melt crystallization in cooling stages;(ii)the metamorphic P-T paths with isobaric cooling under 6-7 kb for the Hengshan and Fuping granulites suggest their prolonged stay in the middle-lower crust;and(iii)the obtained metamorphic age data show a continuous distribution from 1.95 to 1.80 Ga.Thus,an alternative tectonic scenario for the Hengshan-Wutai-Fuping region involves:(i)formation of a proto-NCC at ~2.5 Ga;(ii)back-arc extension during 2.35-2.0 Ga resulting in bimodal magmatism and sedimentation in rifting basins on an Archean basement;(iii)a crustal thickening event in the extended region resulting in a kyanitetype metamorphism at ~1.95 Ga,and(iv) uplifting and cooling of the thickened crust from 1.93 to 1.80 Ga.Chunjing Wei Jiahui Qian Xiwen Zhou 2014Geoscience Frontiers2014,5,4:32
2Expression of OTUB1 in hepatocellular carcinoma and its effects on HCC cell migration and invasion显示文摘Qinggan Ni Jiahui Chen Xia Li Xiaodong Xu Nannan Zhang Ang Zhou Bin Zhou Qian Lu Zhong Chen 2017Acta Biochimica et Biophysica Sinica2017,49,8:9
3Diclofop-methyl affects microbial rhizosphere community and induces systemic acquired resistance in rice显示文摘Diclofop-methyl(DM),a widely used herbicide in food crops,may partly contaminate the soil surface of natural ecosystems in agricultural area and exert toxic effects at low dose to nontarget plants.Even though rhizosphere microorganisms strongly interact with root cells,little is known regarding their potential modulating effect on herbicide toxicity in plants.Here we exposed rice seedlings(Xiushui 63) to 100 μg/L DM for 2 to 8 days and studied the effects of DM on rice rhizosphere microorganisms,rice systemic acquired resistance(SAR) and rice-microorganisms interactions.The results of metagenomic 16 S rDNA Illumina tags show that DM increases bacterial biomass and affects their community structure in the rice rhizosphere.After DM treatment,the relative abundance of the bacterium genera Massilia and Anderseniella increased the most relative to the control.In parallel,malate and oxalate exudation by rice roots increased,potentially acting as a carbon source for several rhizosphere bacteria.Transcriptomic analyses suggest that DM induced SAR in rice seedlings through the salicylic acid(but not the jasmonic acid) signal pathway.This response to DM stress conferred resistance to infection by a pathogenic bacterium,but was not influenced by the presence of bacteria in the rhizosphere since SAR transcripts did not change significantly in xenic and axenic plant roots exposed to DM.The present study provides new insights on the response of rice and its associated microorganisms to DM stress.Si Chen Xingxing Li Michel Lavoie Yujian Jin Jiahui Xu Zhengwei Fu Haifeng Qian 2017Journal of Environmental Sciences2017,29,1:8
4Expression of reconstructive hF Ⅷ in the hrDNA by using hrDNA targeting vector显示文摘Our lab has constructed a new nonviral vec-tor—hrDNA targeting vector(pHrneo). pHrneo is a human derived vector that can target gene into human ribosomal DNA(hrDNA) locus. In this study, we inserted expression cassette of reconstructive hF Ⅷ (hFVIII-BDDAK39) to pHrneo to construct targeting vector: pHrneo-BDDAK39. Through electroporation of pHrneo-BDDAK39 into HT1080 cells, we identified the homologous recombinants by PCR and Southen blotting, and tested the expression of hFVIII- BDDAK39 in the hrDNA locus. The hFⅧ-BDDAK39 was successfully targeted into hrDNA locus of HT-1080 by pHrneo-BDDAK39, and the efficiency of site-specific inte-gration was 2.0×10?5. hFⅧ-BDDAK39 in hrDNA locus of HT-1080 is found to be able to express efficiently (32±5 ng·106 cells?1·24 h?1). Targeting vector pHrneo-BDDAK39 can find use in gene therapy for hemophilia.LIU Xionghao LIU Mujun SHE Hua WEN Lu XUE Zhigang LIANG Desheng CAI Fang PAN Qian LONG Zhigao WU Lingqian DAI Heping XIA Kun XlA Jiahui 2005Chinese Science Bulletin2005,50,19:5
5Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma显示文摘Human ribosomal DNA (hrDNA) target- ing vector (pHrn) is one of the human derived vectors, which was devised by our lab and has got patent authority. To investigate its effect on gene therapy for hepatocellular carcinoma, a double suicide fusion gene expression cassette, CDUPRT/GFP controlled by a synthetic CMV enhancer-enhanced hTERT promoter (CeTp) which was determined by luciferase assays was constructed in pHrn backbone, creating an expression vector pHr-CeTpCDUPRT/GFP. After transfer of plasmid to hepatocellular carcinoma cell line Bel7402 in vitro, the transfection efficiency reached 30%―50% by using flow cytometer. The expression of CDUPRT/GFP was detected by RT-PCR and Western Blotting. After the administra- tion of 5-FC, high performance liquid chromatography (HPLC) was applied to examine the level of 5-FU in supernatant, resulting in a concentration of 60.15 μg/mL. The Methylthiazolyl tetrazolium (MTT) assay was then utilized to investigate the antitumor effect of pHr-CeTpCDUPRT/GFP in Bel7402 cells, and rela- tive cell survival of 60%-35% was observed after 5-FC treatment. In vivo experiments, the nude mouse model of hepatocellular carcinoma was constructed and in situ gene therapy was performed. The results indicated the tumor growth of treatment group was obviously suppressed, and some even shrank, whenthe vectors and prodrugs were injected continuously. The expression of CDUPRT in tumor tissues was also identified by RT-PCR, and the concentration of 5-FU was 7.694 μg/mL in blood serum using HPLC detection. Then the pathological section of tumor tissues revealed significant tumor cell necrosis. All of these results provide important experiment evidences of the gene therapy for hepatocellular carcinoma with the utilization of our vectors.WANG Lina XUE Zhigang LI Zhuo XUE Jinfeng LIU Xionghao PAN Qian LONG Zhigao CAI Fang WU Lingqian DAI Heping XIA Kun LIANG Desheng XIA Jiahui 2006Chinese Science Bulletin2006,51,19:3
6Functional analysis of three genetic disorder related PITX2 mutants显示文摘The autosome dominant disorders, ring dermoid of the cornea (RDC), iris hypolasia (IH) and Axenfeld-Rieger syndrome (ARS), are allelic disor- ders, as all three can result from mutations of the transcriptional factor PITX2. Among three disorder phenotypes, ARS is the most severe, IH is milder than ARS, and RDC is the mildest. Missense muta- tions of the PITX2 homeodomain identified in RDC (R62H), IH (R84W) and ARS patients (T68P) were introduced into PITX2 cDNA by site-directed mutagenesis. PITX2 mutant proteins expressed in eucaryotic cells were stable and localized to the nu- cleus. Analysis of these mutant PITX2 proteins by DNA-binding shift and transactivation studies dem- onstrated that the R62H had the most activity in both studies, and the R84W still retained somewhat func- tions, whereas the T68P proved to be non-functional. These results are consistent with previous hypothesis that varying amount of residual PITX2 mutant activity could underline the severity of these phenotypes.WANG Guo LIU Xiaoping PAN Qian LIANG Desheng WU Qianling DAI Heping ZHU Feizhou XIA Kun XIA Jiahui 2006Chinese Science Bulletin2006,51,2:3
7Silica nanoparticle is a possible safe carrier for gene therapy显示文摘In order to develop a safe and effective gene therapy carrier, some toxicological and biodynamical ex- periments were carried out on silica nanoparticles (SiNPs). First we prepared SiNPs with appropriate portions of cyclo- hexane, deionized water and ethyl silicate, and then trans- fected the modified SiNPs and GFP plasmid DNA complex into the HT1080 cells to test the effectiveness of transfection for gene therapy. At the same time, we injected the SiNPs into a number of mice through tail vein. Then we made the mice crossed to evaluate the acute, long-term and reproduc- tive toxicity. In vivo distribution analysis and pathological examination were made on both adult mice and their off- spring. SiNPs were uniform and had an average diameter of 40 nm, and the modified SiNPs carried exogenous DNA molecules into target cells and the transferred GFP fusion gene was effectively expressed in the cells. The SiNPs injected via tail vein were widely distributed in almost all of tissues, and the injected mice had the ability to reproduce normally. The in vivo and in vitro results of this study clearly show that SiNPs can be used as a safe and effective carrier for gene transfection and gene therapy.XUE Zhigang LIANG Desheng LI Yumei LONG Zhigao PAN Qian LIU Xionghao WU Lingqian ZHU Shaihong CAI Fang DAI Heping TANG Baisha XIA Kun XIA Jiahui 2005Chinese Science Bulletin2005,50,20:2
8Identification and characterization of human neuronal voltage-gated calcium channel gamma 3 subunit gene显示文摘By homologous expressed sequence tag (EST) searching, one EST (GenBank: W29095) was obtained, which shows 75% identity in 435 bp overlap with the coding sequence of mouse Cacng2 gene. A 1 545 bp cDNA fragment was obtained from the nested polymerase chain reaction (PCR) and rapid applification of cDNA end (RACE) reaction in the human brain prefrontal cortex cDNA library and the human brain Ready cDNA with the primers designed on W29095. The fragment contained a 948-bp open reading frame (ORF) encoding 315 amino acids, and was named CACNG3. As it was identical to a BAC clone (GenBank: AC004125) from chromosome 16p12-p13.1, the CACNG3 gene was mapped to human chromosome 16p12-p13.1, and the coding region was composed of 4 exons. Reverse transcription PCR (RT-PCR) analysis showed that the CACNG3 gene expressed in human adult brain and fetal brain. Single strand comformation polymorphism (SSCP) analysis was performed in 3 pedigrees with autosomal recessive retinitis pigmentosa, 8 pedigrees with autosomalJiahui Xia Huali Zhang Dongsheng Tang Xixiang Tang Heping Dai Qian Pan Zhigao Long Xiaodong Liao 2000Chinese Science Bulletin2000,45,23:2
9Cigarette smoke promotes oral leukoplakia via regulating glutamine metabolism and M2 polarization of macrophage显示文摘Oral immunosuppression caused by smoking creates a microenvironment to promote the occurrence and development of oral mucosa precancerous lesions.This study aimed to investigate the role of metabolism and macrophage polarization in cigarette-promoting oral leukoplakia.The effects of cigarette smoke extract(CSE)on macrophage polarization and metabolism were studied in vivo and in vitro.The polarity of macrophages was detected by flow cytometric analysis and q PCR.Liquid chromatography-mass spectrometry(LC-MS)was used to perform a metabolomic analysis of Raw cells stimulated with CSE.Immunofluorescence and flow cytometry were used to detect the polarity of macrophages in the condition of glutamine abundance and deficiency.Cell Counting Kit-8(CCK-8),wound-healing assay,and Annexin V-FITC(fluorescein isothiocyanate)/PI(propidium iodide)double-staining flow cytometry were applied to detect the growth and transferability and apoptosis of Leuk-1 cells in the supernatant of Raw cells which were stimulated with CSE,glutamine abundance and deficiency.Hyperkeratosis and dysplasia of the epithelium were evident in smoking mice.M2 macrophages increased under CSE stimulation in vivo and in vitro.In total,162 types of metabolites were detected in the CSE group.The metabolites of nicotine,glutamate,arachidic acid,and arginine changed significantly.The significant enrichment pathways were also selected,including nicotine addiction,glutamine and glutamate metabolism,and arginine biosynthesis.The results also showed that the supernatant of Raw cells stimulated by CSE could induce excessive proliferation of Leuk-1 and inhibit apoptosis.Glutamine abundance can facilitate this process.Cigarette smoke promotes oral leukoplakia via regulating glutamine metabolism and macrophage M2 polarization.Yanan Zhu Shuo Zhang Jiahui Sun Tingting Wang Qin Liu Guanxi Wu Yajie Qian Weidong Yang Yong Wang Wenmei Wang 2021International Journal of Oral Science2021,13,3:2
10Environmental pollution,a hidden culprit for health issues显示文摘The environmental and health impacts from the massive discharge of chemicals and subsequent pollution have been gaining increasing public concern.The unintended exposure to different pollutants,such as heavy metals,air pollutants and organic chemicals,may cause diverse deleterious effects on human bodies,resulting in the incidence and progression of different diseases.The article reviewed the outbreak of environmental pollution-related public health emergencies,the epidemiological evidence on certain pollution-correlated health effects,and the pathological studies on specific pollutant exposure.By recalling the notable historical life-threatening disasters incurred by local chemical pollution,the damning evidence was presented to criminate certain pollutants as the main culprit for the given health issues.The epidemiological data on the prevalence of some common diseases revealed a variety of environmental pollutants to blame,such as endocrine-disrupting chemicals(EDCs),fine particulate matters(PMs)and heavy metals.The retrospection of toxicological studies provided illustrative clues for evaluating ambient pollutant-induced health risks.Overall,environmental pollution,as the hidden culprit,should answer for the increasing public health burden,and more efforts are highly encouraged to strive to explore the cause-and-effect relationships through extensive epidemiological and pathological studies.Hanqing Xu Yang Jia Zhendong Sun Jiahui Su Qian S.Liu Qunfang Zhou Guibin Jiang 2022Eco-Environment & Health2022,1,1:1
11A high-efficiency grid-tie battery energy storage system显示文摘Qian Hao Zhang Jiahui Lai J S 0,,03:1
12Effects of krill oil on serum lipids of hyperlipidemic rats and human SW480 cells显示文摘ZHU Jiajin SHI Jiahui QIAN Wenbin 2008Lipids in Health and Disease2008,7,:1
13Zircon U-Pb Ages for TTG Gneiss and a Concomitant Felsic Vein from the South Hengshan Complex,Trans-North China Orogen:New Evidence for Late Archean Metamorphism显示文摘Objective The Trans-North China Orogen(TNCO)is widely considered as a Paleoproterozoic(1.95-1.85 Ga)continental collisional belt along which the Eastern and Western blocks amalgamated to form the North China Craton.In recent years,several late Archean(~2.50 Ga)metamorphic ages were reported in this belt(Yang et al.,2015;Xiao et al.,2019;Wang et al.,2020),arousing widespread discussion on late Archean metamorphism.In the South Hengshan Complex(SHC)of the TNCO,an interesting outcrop has been discovered(Fig.1),which shows a clear truncation relationship between a strongly deformed tonal ite-trondhjemite-granodiorite(TTG)gneiss and a weakly deformed mafic dyke with'red-eye socket'texture(Fig.la).QIAN Jiahui LI Shun 2021Acta Geologica Sinica(English Edition)2021,95,5:1
14Infinite-and K-Step Opacity Verification of Discrete-Event Systems Under Nondeterministic Observations显示文摘In some practical applications modeled by discrete-event systems(DES),the observations of events may be no longer deterministic due to sensor faults/failures,packet loss,and/or measurement uncertainties.In this context,it is interesting to reconsider the infinite-step opacity(∞-SO)and K-step opacity(K-SO)of a DES under abnormal conditions as mentioned.In this paper,the authors extend the notions of∞-SO and K-SO defined in the standard setting to the framework of nondeterministic observations(i.e.,the event-observation mechanism is state-dependent and nondeterministic).Obviously,the extended notions of∞-SO and K-SO are more general than the previous standard ones.To effectively verify them,a matrix-based current state estimator in the context of this advanced framework is constructed using the Boolean semi-tensor product(BSTP)technique.Accordingly,the necessary and sufficient conditions for verifying these two extended versions of opacity are provided as well as their complexity analysis.Finally,several examples are given to illustrate the obtained theoretical results.CHU Qian ZHANG Jiahui HAN Xiaoguang LI Zhiwu CHEN Zengqiang 2023Journal of Systems Science & Complexity2023,36,5:0
15New Discovery of ~1866 Ma High-temperature Mylonite in the Helanshan Complex: Marking a Late-stage Ductile Shearing in the Khondalite Belt, North China Craton显示文摘Objective The Khondalite Belt is a nearly E–W-trending Paleoproterozoic Himalayan-type orogen in the Western Block of the North China Craton. It is regarded as a result of the collision between the northern Yinshan Block and the southern Ordos Block at ~1.95 Ga, which led to the formation of the united basement of the Western Block(e.g., Zhao et al., 2005;Yin et al., 2011). The Khondalite Belt has undergone long-term continent-continen collisional orogenic processes, and a remarkable series of late-stage orogen-parallel ductile shear zones developed(e.g., Huang et al., 2013;Gong et al., 2014).QIAO Hengzhong YIN Changqing ZHANG Jian QIAN Jiahui WU Shangjing 2021Acta Geologica Sinica(English Edition)2021,95,4:0
16Atomic-resolution characterization on the structure of strontium doped barium titanate nanoparticles显示文摘Ferroelectric barium titanate nanoparticles(BTO NPs)may play critical roles in miniaturized passive electronic devices such as multi-layered ceramic capacitors.While increasing experimental and theoretical understandings on the structure of BTO and doped BTO have been developed over the past decade,the majority of the investigation was carried out in thin-film materials;therefore,the doping effect on nanoparticles remains unclear.Especially,doping-induced local composition and structure fluctuation across single nanoparticles have yet to be unveiled.In this work,we use electron microscopy-based techniques including high-angle annular dark-field scanning transmission electron microscopy(HAADF-STEM),integrated differential phase contrast(iDPC)-STEM,and energy dispersive X-ray spectroscopy(EDX)mapping to reveal atomically resolved chemical and crystal structure of BTO and strontium doped BTO nanoparticles.Powder X-ray diffraction(PXRD)results indicate that the increasing strontium doping causes a structural transition from tetragonal to cubic phase,but the microscopic data validate substantial compositional and microstructural inhomogeneities in strontium doped BTO nanoparticles.Our work provides new insights into the structure of doped BTO NPs and will facilitate the materials design for next-generation high-density nano-dielectric devices.Haoyu Jiang Jizhen Qi Dongchang Wu Wei Lu Jiahui Qian Haifeng Qu Yixiao Zhang Pei Liu Xi Liu Liwei Chen 2021Nano Research2021,14,12:0
17Effect of wall jet on the bubble dynamics near arigid wall显示文摘The bubble dynamic near a rigid wall with a wall jet was investigated by codynamics(CFD)method with the volume of fluid(VOF)model,which had been validated by vious experimental data.The effects of different velocities of the wall jet and ditances on the bubble dynamics were studied.The results show that the bubble is squjet due to more force added on the bubble.When the velocity of the wall jet increa,the wall anthe pressure along the wall at collapse time increase because of the extra push indAs the stand-off distance increases,the pressure along the wall first increases then decrethe distance from the bubble to the wall increases.LU Jie QIAN Zhongdong GUO Zhiwei WANG Chihang SU Jiahui 2017排灌机械工程学报2017,35,6:0
18Different patterns of association between white matter microstructure and plasma unsaturated fatty acids in those with high risk for psychosis and healthy participants显示文摘Background Disrupted white matter(WM)microstructure has been commonly identified in youth at clinical high risk(CHR)for psychosis.Several lines of evidence suggest that fatty acids,especially unsaturated fatty acids(UFAs),might play a crucial role in the WM pathology of early onset psychosis.However,evidence linking UFA and WM microstructure in CHR is quite sparse.Aims We investigated the relationship between the plasma UFA level and WM microstructure in CHR participants and healthy controls(HC).Methods Plasma fatty acids were assessed and diffusion tensor imaging(DTI)data were performed with tract-based spatial statistics(TBSS)analysis for 66 individuals at CHR for psychosis and 70 HC.Results Both the global and regional diffusion measures showed significant between-group differences,with decreased fractional anisotropy(FA)but increased mean diffusivity(MD)and radial diffusivity(RD)found in the CHR group compared with the HC group.On top of that,we found that in the HC group,plasma arachidic acid showed obvious trend-level associations with higher global FA,lower global MD and lower global RD,which regionally spread over the corpus callosum,right anterior and superior corona radiata,bilateral anterior and posterior limb of the internal capsule,and bilateral superior longitudinal fasciculus.However,there were no associations between global WM measures and any UFA in the CHR group.Conversely,we even found negative associations between arachidic acid levels and regional FA values in the right superior longitudinal fasciculus and right retrolenticular part of the internal capsule in the CHR group.Conclusions Compared with the HC group,CHR subjects exhibited a different pattern of association between WM microstructure and plasma UFA,with a neuroprotective effect found in the HC group but not in the CHR group.Such discrepancy could be due to the excessively upregulated UFAs accumulated in the plasma of the CHR group,highlighting the role of balanced plasma-membrane fatty acids homeostasis in WM development.Wenjun Su Zhixing Li Lihua Xu Jiahui Zeng Yingying Tang Xiaochen Tang Yanyan Wei Qian Guo Tianhong Zhang Jijun Wang 2022General Psychiatry2022,35,2:0
19Newly discovered youngest UHT metamorphism on Earth,Western Sulawesi,Indonesia显示文摘Ultrahigh-temperature(UHT)metamorphism represents an extreme crustal thermal event with peak conditions exceeding 900C at 7–13 kbar.In the modern-style plate tectonic system,records of the UHT metamorphism are relatively rare due to the secular cooling of Earth.In the Palu region of Western Sulawesi,we newly discovered a series of HT-UHT metamorphic rocks including amphibolite,granulite,eclogites and gneiss.Of them,two granulite samples(18CS14-2,18CS14-4)with high garnet content(>50 mol%)are chosen for petrographic observation,phase equilibrium modelling,and zircon U-Pb dating.These rocks are characterized by a relic M1 assemblage of Grt+Ky+Bt+Rt and a M2 assemblage of Grt+Sil+Pl+Spl+Crd±Qtz+Ilm+melt.Phase equilibrium modelling based on effective bulk compositions yields UHT conditions of 7.2–8.5 kbar/940–1080C(18CS14-2)and 7.0–7.3 kbar/1000–1040C(18CS14-4).U-Pb analysis reveals two generations of metamorphic zircon with evolving REE content that is intimately related to garnet growth and decomposition.Zircon age of 36–5.3 Ma is ascribed to syn-to post-M1 metamorphism,whereas the young zircon age of 5.1–3.8 Ma is linked to syn-and post-M2 stage.The UHT metamorphism was probably the consequence of the upwelling of asthenospheric mantle triggered by post-collisional delamination of lithosphere in the MiocenePliocene(ca.5 Ma).It could represent the youngest known UHT metamorphism on Earth.Juiyen Hsia Jian Zhang Guochun Zhao Jiahui Qian Jin Liu Min Sun Changqing Yin Peng Gao Welson Weisheng Xian Minjie Guo 2023Geoscience Frontiers2023,14,2:0
20The opportunistic relaying scheme design and symbolerror rate analysis for PLC networks in smart homes显示文摘Dear editor,Power line communication(PLC)is treated as a retrofit technology,transmitting messages via conductors without extra infrastructure,which reduces the cost of implementation and maintenance[1].Therefore,PLC is extensively adopted in smart grid applications.However,PLC encounters a hostile communication environment caused by the fast signal attenuation and severe impulsive noise[2].These factors limit the reliability of PLC networks in long-distance transmission.In general,reliability is a key factor for PLC networks,measured with the symbol error rate(SER).Thus,the research on SER analysis for PLC networks has attracted great attention.Linlin SUN Jiahui YAN Yuwen QIAN Feng SHU Xiangwei ZHOU 2021Science China(Information Sciences)2021,64,8:0
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