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| 1 | An Investigation of Oxidative DNA Damage in Pharmacy Technicians Exposed to Antineoplastic Drugs in Two Chinese Hospitals Using The Urinary 8-OHdG Assay显示文摘Objective To investigate oxidative DNA damage in pharmacy technicians preparing antineoplastic drugs at the PIVAS (Pharmacy Intravenous Admixture Service) in two Chinese hospitals.Methods Urinary 8-OHdG served as a biomarker.5-Fluorouracil (5-FU) concentrations in air,masks and gloves were determined.The spill exposure of each PIVAS technician to antineoplastic drugs was investigated.Eighty subjects were divided into exposed group I,II,and control group I,II.Results 5-FU concentration ratios for gloves and masks in exposed group I were significantly higher than those in exposed group II (P<0.05 or P<0.01).The average urinary 8-OHdG concentrations in exposed group I,control group I,exposed group II,and control group II were 14.69±0.93,10.68±1.07,10.57±0.55,and 11.96±0.73 ng/mg Cr,respectively.Urinary 8-OHdG concentration in exposed group I was significantly higher than that in control group I or that in exposed group II (P<0.01).There was a significant correlation between urinary 8-OHdG concentrations and spill frequencies per technician (P<0.01).Conclusion There was detectable oxidative DNA damage in PIVAS technicians exposed to antineoplastic drugs.This oxidative DNA damage may be associated with their spill exposure experience and contamination of their personal protective equipment. | HUANG Yu Wen JIAN Le ZHANG Mei Bian ZHOU Quan YAN Xiao Feng HUA Xu Dong ZHOU Ying HE Ji Liang | 2012 | Biomedical and Environmental Sciences2012，25，1： | 30 |
| 2 | Indoor Allergen Levels and Household Distributions in Nine Cities Across China显示文摘Objective Chinese allergic subjects have high levels of sensitization to house dust mite(HDM) and other indoor allergens. This study quantifies common indoor allergen levels in Chinese households. Methods Dust samples were collected from nine cities. Major allergens Der p 1 and Der f 1 from Dermatophagoides pteronyssinus and D. farinae, and specific antigens of Blomia tropicalis, Tyrophagus putrescentiae, Acarus siro, and cockroach species Blattella germanica and Periplaneta americana were measured by ELISA. Results HDM allergens were found in dust samples from bedding in 95% of the Chinese households. The median levels varied from <0.006 to 9.2 μg/g of dust, depending on the city. The percentages of households having HDM allergen levels associated with the risk of developing allergy sensitization and asthma were 65% and 25%, respectively. Specific antigens of the storage mite and cockroach were only found in samples from the southern and tropical regions of China. Levels of mite allergens were generally higher in samples from bedding compared to samples from the living room, even for storage mites, whereas levels of cockroach antigens were higher in the living room samples. Conclusion HDM allergens are present in bedding dust samples from most Chinese households. Cities in southern and central China have relatively high levels of HDM major allergens compared to cities in northern and western China. Antigens of storage mites and cockroaches are not as common as HDM allergens. | ZHENG Yi Wu LAI Xu Xin ZHAO De Yu ZHANG Chun Qing CHEN Jian Jun ZHANG Luo WEI Qing Yu CHEN Shi LIU En Mei NORBACK Dan GJESING Birgitte ZHONG Nan Shan SPANGFORT D.Michael | 2015 | Biomedical and Environmental Sciences2015，28，10： | 24 |
| 3 | Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells显示文摘AIM: To study the capacity of bone marrow mesenchymal stem cells (BM-MSCs) trans-differentiating into islet-like cells and to observe the effect of portal vein transplantation of islet-like cells in the treatment of streptozotocin-induced diabetic rat. METHODS: BM-MSCs were isolated from SD rats and induced to differentiate into islet-like cells under defined conditions. Differentiation was evaluated with electron microscopy, RT-PCR, immunofluorescence and flow cytometry. insulin release after glucose challenge was tested with ELiSA. Then allogeneic islet-like cells were transplanted into diabetic rats via portal vein. Blood glucose levels were monitored and islet hormones were detected in the liver and pancreas of the recipient by immunohistochemistry. RESULTS: BM-MSCs were spheroid adherent monolayers with high CD90, CD29 and very low CD45 expression. Typical islet-like cells clusters were formed after induction. Electron microscopy revealed that secretory granules were densely packed within the cytoplasm of the differentiated cells. The spheroid cells expressed islet related genes and hormones. The insulin-positive cells accounted for 19.8% and mean fluorescence intensity increased by 2.6 fold after induction. The cells secreted a small amount of insulin that was increased 1.5 fold after glucose challenge. After transplantation, islet-like cells could locate in the liver expressing islet hormones and lower the glucose levels of diabetic rats during d 6 to d 20.CONCLUSION: Rat BM-MSCs could be transdifferentiated into islet-like cells in vitro . Portal vein transplantation of islet-like cells could alleviate the hyperglycemia of diabetic rats. | Xiao-Hong Wu Cui-Ping Liu Kuan-Feng Xu Xiao-Dong Mao Jian Zhu Jing-Jing Jiang Dai Cui Mei Zhang Yu Xu Chao Liu | 2007 | World Journal of Gastroenterology2007，13，24： | 23 |
| 4 | Additive Benefits of Twice Forest Bathing Trips in Elderly Patients with Chronic Heart Failure显示文摘长期的心失败(CHF ) ,源于各种各样的心血管的疾病(CVD ) 的后果的临床的症候群,逐渐地正在成为病态和死亡的一个全球原因。我们更早证明了洗旅行的一个 4 天的森林能在病人上向附属治疗学的影响提供 CHF。为了进一步调查影响和森林洗澡的最佳的频率的持续时间,与 CHF 在病人摔倒,我们招募了经历了在 4 个星期以后再洗旅行的第一个森林并且也就是,随机把他们分成了二个组的那些对象城市的控制组(城市)和洗组(森林)的森林。在洗旅行的第二个 4 天的森林以后,我们象氧化应力一样在大脑 natriuretic 肽层次,心失败的 biomarker,和稀释煽动性的回答观察了稳定的衰落。因此,这探索研究表明了添加剂好处两次,森林洗澡与 CHF 在老病人摔倒,它能进一步为在 CVD 分析如此的干预的效果铺平道路。 | MAO Gen Xiang CAO Yong Bao YANG Yan CHEN Zhuo Mei DONG Jian Hua CHEN Sha Sha WU Qing LYU Xiao Ling JIA Bing Bing YAN Jing WANG Guo Fu | 2018 | Biomedical and Environmental Sciences2018，31，2： | 18 |
| 5 | Advanced Space-based Solar Observatory(ASO-S):an overview显示文摘The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C. | Wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li Yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng Yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua Yan Jun Lin Wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao Yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li Wei Chen You-Ping Li Ying-Na Su Hai-Yan Wu Mei Gu Lei Huang Xue-Jun Tang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 17 |
| 6 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 7 | Comparison of combined phacotrabeculectomy with trabeculectomy only in the treatment of primary angle-closure glaucoma显示文摘 | WANG Mei FANG Min BAI Yu-jing ZHANG Wei-zhong LIN Ming-kai LIU Bing-qian HAO Yuan-tao LING Yun-lan ZHUO Ye-hong GE Jian | 2012 | Chinese Medical Journal2012，，8： | 15 |
| 8 | Molecular Epidemiological Characteristics of Streptococcus pyogenes Strains Involved in an Outbreak of Scarlet Fever in China,2011显示文摘Objective To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.Methods Seventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011.Emm genotyping,pulsed-field gel electrophoresis (PFGE),superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.Results A total of 4 different emm types were identified.Emm12 was the most prevalent type which contained four predominating PFGE patterns corresponding to four different virulence and superantigen profiles.Emm12 (79.7%) and emml (14.9%) accounted for approximately 94%of all the isolates.The speA gene was all negative in emm12 isolates and positive in emml isolates.All strains were resistant to erythromycin,and 89.4%of them were resistant to erythromycin,tracycline,and clindamycin simultaneously.Conclusion Several highly diversified clones with a high macrolide resistance rate comprise a predominant proportion of circulating strains,though no new emm type was found in this outbreak.The data provide a baseline for further surveillance of scarlet fever,which may contribute to the explanation of the outbreak and development of a GAS vaccine in China. | YOU Yuan Hai SONG Yan Yan YAN Xiao Mei WANG Hai Bin ZHANG Meng Han TAO Xiao Xia Li Lei Lei ZHANG Yu Xin JIANG Xi Hong ZHANG Bing Hua ZHOU Hao XIAO Di JIN Lian Mei FENG Zi Jian LUO Feng Ji ZHANG Jian Zhong | 2013 | Biomedical and Environmental Sciences2013，26，11： | 15 |
| 9 | NK cell-based cancer immunotherapy: from basic biology to clinical application显示文摘Natural killer(NK) cells, which recognize and kill target cells independent of antigen specificity and major histocompatibility complex(MHC) matching, play pivotal roles in immune defence against tumors. However, tumor cells often acquire the ability to escape NK cell-mediated immune surveillance. Thus, understanding mechanisms underlying regulation of NK cell phenotype and function within the tumor environment is instrumental for designing new approaches to improve the current cell-based immunotherapy. In this review, we elaborate the main biological features and molecular mechanisms of NK cells that pertain to regulation of NK cell-mediated anti-tumor activity. We further overview current clinical approaches regarding NK cell-based cancer therapy, including cytokine infusion, adoptive transfer of autologous or allogeneic NK cells, applications of chimeric antigen receptor(CAR)-expressing NK cells and adoptive transfer of memory-like NK cells. With these promising clinical outcomes and fuller understanding the basic questions raised in this review, we foresee that NK cell-based approaches may hold great potential for future cancer immunotherapy. | LI Yang YIN Jie LI Ting HUANG Shan YAN Han LEAVENWORTH Jian Mei WANG Xi | 2015 | Science China(Life Sciences)2015，58，12： | 14 |
| 10 | Nasopharyngeal carcinoma:Advances in genomics and molecular genetics显示文摘Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma that arises in the epithelial lining of the nasopharynx [1]. This neoplasm has a notable ethnic and geographic distribution,being of high prevalence in southern China but rare in other parts of the world [2]. | ZENG ZhaoYang HUANG HongBin ZHANG WenLing XIANC Bo ZHOU Ming ZHOU YanHong MA Jian YI Mei LI XiaYu LI XiaoLing XIONG Wei LI GuiYuan | 2011 | Science China(Life Sciences)2011，54，10： | 13 |
| 11 | Effect of fixing distal radius fracture with volar locking palmar plates while preserving pronator quadratus显示文摘 | Fan Jian Chen Kai Zhu Hui Jiang Bo Yuan Feng Zhu Xiaozhong Mei Jiong Yu Guangrong | 2014 | Chinese Medical Journal2014，，16： | 13 |
| 12 | Dual-targeting and microenvironment-responsive micelles as a gene delivery system to improve the sensitivity of glioma to radiotherapy显示文摘Dbait is a small double-stranded DNA molecule that has been utilized as a radiosensitizer to enhance the sensitivity of glioma to radiotherapy(RT). However, there is no effective drug delivery system to effectively overcome the blood–brain barrier(BBB). The aim of this study was to develop a gene delivery system by using the BBB and glioma dual-targeting and microenvironment-responsive micelles(ch-Kn(s-s)R8-An) to deliver Dbait into glioma for RT. Angiopep-2 can target the low-density lipoprotein receptor-related protein-1(LRP1) that is overexpressed on brain capillary endothelial cells(BCECs) and glioma cells. In particular, due to upregulated matrix metalloproteinase 2(MMP-2) in the tumor microenvironment, we utilized MMP-2-responsive peptides as the enzymatically degradable linkers to conjugate angiopep-2. The results showed that ch-Kn(s-s)R8-An micelles maintained a reasonable size(80–160 nm) with a moderate distribution and a decreased mean diameter from the cross-linking as well as exhibited low critical micelle concentration(CMC) with positive surface charge, ranging from 15 to40 mV. The ch-K5(s-s)R8-An/pEGFP showed high gene transfection efficiency in vitro, improved uptake in glioma cells and good biocompatibility in vitro and in vivo. In addition, the combination of ch-K5(s-s)R8-An/Dbait with RT significantly inhibited the growth of U251 cells in vitro. Thus, ch-K5(s-s)R8-An/Dbait may prove to be a promising gene delivery system to target glioma and enhance the efficacy of RT on U251 cells. | Xiuxiu Jiao Yuan Yu Jianxia Meng Mei He Charles Jian Zhang Wenqian Geng Baoyue Ding Zhuo Wang Xueying Ding | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 13 |
| 13 | Association of Optic Radiation Integrity with Cortical Thickness in Children with Anisometropic Amblyopia显示文摘Previous studies have indicated regional abnormalities of both gray and white matter in amblyopia.However, alterations of cortical thickness associated with changes in white matter integrity have rarely been reported.In this study, structural magnetic resonance imaging and diffusion tensor imaging(DTI) data were obtained from 15 children with anisometropic amblyopia and 15 age- and gender-matched children with normal sight. Combining DTI and surface-based morphometry, we examined a potential linkage between disrupted white matter integrity and altered cortical thickness. The fractional anisotropy(FA) values in the optic radiations(ORs) of children with anisometropic amblyopia were lower than in controls(P \ 0.05). The cortical thickness in amblyopic children was lower than controls in the following subregions: lingual cortex, lateral occipitotemporal gyrus, cuneus, occipital lobe, inferior parietal lobe, and temporal lobe(P \ 0.05, corrected), but was higher in the calcarine gyrus(P \ 0.05, corrected). Node-by-node correlation analysis of changes in cortical thickness revealed a significant association between a lower FA value in the OR and diminished cortical thickness in the following subregions:medial lingual cortex, lateral occipitotemporal gyrus, lateral, superior, and medial occipital cortex, and lunate cortex. We also found a relationship between changes of cortical thickness and white matter OR integrity in amblyopia. These findings indicate that developmental changes occur simultaneously in the OR and visual cortex in amblyopia, and provide key information on complex damage of brain networks in anisometropic amblyopia. Our results also support the hypothesis that the pathogenesis of anisometropic amblyopia is neurodevelopmental. | Shun Qi Yun-Feng Mu Long-Biao Cui Rong Li Mei Shi Ying Liu Jun-Qing Xu Jian Zhang Jian Yang Hong Yin | 2016 | Neuroscience Bulletin2016，32，1： | 12 |
| 14 | Orthogonal Test Design for Optimization of the Extraction of Flavonid from the Fructus Gardeniae显示文摘Objective It is imperative to provide some consistent experimental results for the extraction of flavonid from Fructus Gardeniae.Methods The key extraction parameters that influenced the yield of flavonid from Fructus Gardeniae were optimized by employing an orthogonal experiment [L9(3)4],including the ratio of buffer solution (Na2B4O7·10H2O) to raw material,concentration of Fructus Gardeniae in extracting solution,extraction time and pH of buffer solution.An UV/Vis detector was used to perform the qualitative and quantitative analyses of the extracted flavonid with the using of the standard sample.Results The maximum extraction yield of the crude extract was 5.0533 (mg/g) after 20 min when the mass ratio of Na2B4O7·10H2O to raw material was 0.4%,the concentration of Fructus Gardeniae in the extraction solution was 1/12 (g/mL),and pH of buffer solution was 4.5.The positive reactions to the Molish and HCl‐Mg tests suggested that the extracted compound was flavonoid,and FTIR measurements also identified the presence of flavonoid in the extracts.Conclusion This work is expected to provide a basis for further research,development,and utilization of Fructus gardenia in flavonid extraction. | CAI Ding Jian SHU Qing XU Bao Quan PENG Li Mei HE Yan | 2011 | Biomedical and Environmental Sciences2011，24，6： | 12 |
| 15 | Ginsenoside-Rg_6, a Novel Triterpenoid Saponin from the Stem-Leaves of Panax ginseng C. A. Mey.显示文摘A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2 2. On the basis of chemical and physicochemical evidence , the structure of ginsenoside-Rg6 have been elucidated as 6-O-(-L-rhamnosyl-(1?2)-(-D-glucopyranosyl-dammarane-(E)-20(22), 24-diene-3(, 6(, 12(-triol. | Xiu Wei YANG, Long Yun LI, Jian Ming TIAN, Zhi Wei ZHANG, Jin Mei YE, Wei Fang Gu (National Research Laboratory of Natural & Biomimetic Drugs, Peking University, Beijing 100083) (Academy of Chinese Traditional Medicine and Materia Medica of Jilin Provinc | 2000 | Chinese Chemical Letters2000，11，10： | 12 |
| 16 | MicroRNA-191 targets N-deacetylase/N-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803显示文摘作为基因表示的 post-transcriptional 管理者的一个家庭, microRNAs (miRNAs ) 控制包括房间区别,增长和 apoptosis 的生物进程的一个宽数组,和 miRNAs 的 dysregulation 是癌症的一个特点。这里,我们发现 microRNA-191 (miR-191 ) 在在人的胃的腺癌房间线 MGC803 和人的胃的癌症纸巾的高表示的水平。-2,5-diphenyltetrazolium 溴化物和殖民地形成试金显示出的 3-(4,5-dimethylthiazol-2-yl ) 的结果那 miR-191 能支持房间生长并且压制 MGC803 房间的 apoptosis。N-deacetylase/N-sulfotransferase 1 (NDST1 ) 被证实是提高的绿色边的 miR-191 的直接目标基因荧光灯蛋白质记者实验。NDST1 的 mRNA 和蛋白质层次相反地在 MGC803 房间与 miR-191 被相关,建议由 miR-191 的 NDST1 的否定规定。而且, NDST1 起了一个禁止的作用并且能压制 MGC803 房间增长。我们的调查结果建议 miR-191 能在 MGC803 房间充当 oncogene,并且细胞的功能部分由于它 NDST1 的否定规定。 | Xuejun Shi Shicang SU Jian Long Bing Mei Yajun Chen | 2011 | Acta Biochimica et Biophysica Sinica2011，43，11： | 12 |
| 17 | One-stop strategy for treatment of atrial fibrillation:feasibility and safety of combining catheter ablation and left atrial appendage closure in a single procedure显示文摘Background:Catheter ablation is effective in restoring sinus rhythm and left atrial appendage closure(LAAC)is increasingly used for stroke prevention in patients with atrial fibrillation(AF).We aimed to observe the feasibility and safety of performing AF ablation and LAAC in a single(one-stop)procedure.Methods:Consecutive AF patients who underwent the combined procedure of AF ablation and LAAC with WATCHMAN device between March 2017 and September 2018 were prospectively enrolled.Baseline and intra-procedural parameters were evaluated.Three-month and subsequent 1-year follow-up were performed in all and earlier-enrolled subjects,respectively.Results:A total of 178 AF patients(94 males,68.9±8.1 years)underwent the one-stop procedure with CHA2DS2-VASc score 3.3±1.5 and HAS-BLED score 1.6±1.0,respectively.Pulmonary vein isolation was achieved in all patients while additional linear ablation was applied if the operator deemed necessary,yielding immediate ablation success rate of 98.9%(176/178).In the subsequent LAAC,satisfactory seal(residual leak<5 mm)was achieved in all patients.One stroke and four cardiac perforations occurred peri-operatively.At 3-month follow-up,sinus rhythm and satisfactory seal were maintained in 153/178(86.0%)and 178/178(100%)patients,respectively.One stroke and one delayed cardiac tamponade occurred,while no device-related thrombus or device migration was observed.During the 1-year follow-up for the earlier enrolled subjects,52/72(72.2%)of the patients maintained sinus rhythm.There was no stroke or systemic embolism observed.Conclusion:Combining catheter ablation and LAAC in a single procedure can be successfully and safely performed in non-valvular AF patients of Chinese population. | Mu Chen Zhi-Quan Wang Qun-Shan Wang Jian Sun Peng-Pai Zhang Xiang-Fei Feng Wei Li Ying Yu Bo Liu Bin-Feng Mo Rui Zhang Mei Yang Chang-Qi Gong Ming-Zhe Zhao Yi-Chi Yu Yan Zhao Qiu-Fen Lu Yi-Gang Li | 2020 | Chinese Medical Journal2020，，12： | 11 |
| 18 | A de novo Genome of a Chinese Radish Cultivar显示文摘Here, we report a high-quality draft genome of a Chinese radish(Raphanus sativus) cultivar. This draft contains 387.73 Mb of assembled scaffolds, 83.93% of the scaffolds were anchored onto nine pseudochromosomes and 95.09% of 43 240 protein-coding genes were functionally annotated. 184.75 Mb(47.65%) of repeat sequences was identified in the assembled genome. By comparative analyses of the radish genome against 10 other plant genomes, 2 275 genes in 780 gene families were found unique to R. sativus. This genome is a good reference for genomic study and of great value for genetic improvement of radish. | ZHANG Xiaohui YUE Zhen MEI Shiyong QIU Yang YANG Xinhua CHEN Xiaohua CHENG Feng WU Zhangyan SUN Yuyan JING Yi LIU Bo SHEN Di WANG Haiping CUI Na DUAN Yundan WU Jian WANG Jinglei GAN Caixia WANG Jun WANG Xiaowu LI Xixiang | 2015 | Horticultural Plant Journal2015，1，3： | 10 |
| 19 | Variation in cis-regulation of a NAC transcription factor contributes to drought tolerance in wheat显示文摘Drought is a major environmental factor limiting wheat production worldwide,and developing drought-tolerant cultivars is a central challenge for wheat breeders globally.Therefore,it is important to identify genetic components determining drought tolerance in wheat.In this study,we identified a wheat NAC gene(TaNAC071-A)that is tightly associated with drought tolerance by a genome-wide association study.Knockdown of TaNAC071-A in wheat attenuated plant drought tolerance,whereas its overexpression significantly enhanced drought tolerance through improved water-use efficiency and increased expression of stress-responsive genes.This heightened water-saving mechanism mitigated the yield loss caused by water deficit.Further candidate gene association analysis showed that a 108-bp insertion in the promoter of TaNAC071-A alters its expression level and contributes to variation in drought tolerance among wheat accessions.This insertion contains two MYB cis-regulatory elements(CREs)that can be directly bound by the MYB transcription activator,TaMYBL1,thereby leading to increased TaNAC071-A expression and plant drought tolerance.Importantly,introgression of this 108-bp insertion allele,TaNAC071-AIn-693,into drought-sensitive cultivars could improve their drought tolerance,demonstrating that it is a valuable genetic resource for wheat breeding.Taken together,our findings highlight a major breakthrough in determining the genetic basis underlying phenotypic variation in wheat drought tolerance and showcase the potential of exploiting CRE-containing indels for improving important agronomical traits. | Hude Mao Shumin Li Bin Chen Chao Jian Fangming Mei Yifang Zhang Fangfang Li Nan Chen Tian Li Linying Du Li Ding Zhongxue Wang Xinxiu Cheng Xiaojing Wang Zhensheng Kang | 2022 | Molecular Plant2022，15，2： | 9 |
| 20 | Rapid Detection of Haemophilus influenzae and Haemophilus parainfluenzae in Nasopharyngeal Swabs by Multiplex PCR显示文摘Objective To establish multiplex PCR-based assays for detecting H.influenzae and H.parainfluenzae.And the PCR-based assays were applied to detect the carriage rates of H.influenzae and H.parainfluenzae in nasopharyngeal swab specimens which were collected from healthy children.Methods Multiplex primers for species-specific PCR were designed by using DNAstar soft based on the sequences of 16S rRNA genes from genus Haemophilus to detect H.influenzae and H.parainfluenzae.Results The sensitivity of the 16S rRNA PCR assay for detecting H.influenzae and H.parainfluenzae was 97.53% and 100% respectively,and the specificity was 95.89% and 96.63% respectively.Youden's Index on the ability to detect H.influenzae and H.parainfluenzae was 0.9342 and 0.9663 respectively.666 nasopharyngeal swab specimens were collected from healthy children.The detection rates of H.influenzae and H.parainfluenzae were 14.11% and 16.07% respectively by using isolation and culture methods.The detection rates of H.influenzae and H.parainfluenzae were 43.54% and 57.96% respectively by 16S rRNA PCR assays.The carriage rates of serotypes a,b,c,d,e,f and non-typeable isolates were 0% (0/666),0.15% (1/666),1.20% (8/666),0.15% (1/666),1.20% (8/666),1.80% (12/666),95.50% (636/666) respectively.Conclusion The multiplex PCR assays were very rapid,reliable and feasible methods for detection of H.influenzae and H.parainfluenzae in pharyngeal swab specimens which were compared to conventional isolation and culture methods.95.5% of H.influenzae strains in healthy children were nontypeable.The encapsulated or typable strains were mainly three serotypes which was c,e,and f serotype. | TIAN Guo Zhong ZHANG Li Juan WANG Xiao Lei ZHANG Li LI Shu Feng GU Chang Mei SUN Jian CUI Bu Yun | 2012 | Biomedical and Environmental Sciences2012，25，3： | 8 |
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