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| 1 | Expanding the Range of CRISPR/Cas9 Genome Editing in Rice显示文摘 | Xixun Hu Chun Wang Yaping FU Qing Liu Xiaozhen Jiao Kejian Wang | 2016 | Molecular Plant2016，9，6： | 31 |
| 2 | An atomic force microscopy study of coal nanopore structure显示文摘Coal nanopore structure is an important factor in understanding the storage and migration of absorbed gas in coal. A new method for studying coal nanopore structures is proposed. This idea is based on the nano-level resolution of atomic force microscopy, which can be employed to observe the structural features of coal nanopores clearly, conduct quantitative three-dimensional measurements and obtain structural parameters. Analysis results show that coal nanopores are mainly metamorphic pores and intermolecular pores. The metamorphic pores are commonly rounded and elliptical, increasing quantitatively with the coalification degree. The forms of intermolecular pores change markedly. The average pore size of low-rank coal is bigger than high-rank coal, and the number of intermolecular pores decreases as the coal rank increases. Section analysis effectively characterizes the coal pore microstructure, bearing analysis is a vital approach to measure microporosity, and grain analysis can be employed to study the pore size distribution. Atomic force microscopy is a tool for the in-depth research of coal pore microstructure and the coal-bed methane adsorption mechanism. | YAO SuPing JIAO Kun ZHANG Ke HU WenXuan DING Hai LI MiaoChun PEI WenMing | 2011 | Chinese Science Bulletin2011，56，25： | 30 |
| 3 | Mapping of qGL7-2, a grain length QTL on chromosome 7 of rice显示文摘剩余在米饭染色体上带在二 SSR loci RM11 和 RM134 之间的一个异质接合的片断的异质接合的线(RHL ) 7 从一套 recombinant 被选择从生气 D50 (javanica )/HB277 (indica ) 的生来的线。以前的父母比后者生产长得多的谷物。这种选择的 Selfed 子孙 genotypically 被分析(SSR ) 并且 phenotypically (谷物长度) 。谷物长度在印射的人口是不连续地可变的,允许在 4.8 厘米间隔以内的这 QTL qGL7-2 的放置由 RM351 和 RM234 定义。在这个区域以内的一套新标记被开发,它把 QTL 缩小了到标记 Indel1 和 RM21945 定义的 278 kb 区域。这个区域包含 49 预言的基因。结果也建议为谷物长度的新奇等位基因将被用于标记的应用程序为谷物长度的改进的帮助选择。 | Gaoneng Shao Shaoqing Tang Ju Luo Guiai Jiao Xiangjin Wei Ao Tang Jianli Wu Jieyun Zhuang Peisong Hu | 2010 | Journal of Genetics and Genomics2010，37，8： | 28 |
| 4 | Structure reveals that BAK1 as a co-receptor recognizes the BRIl-bound brassinolide显示文摘 | Yadong Sun Zhlfu Han Jiao Tang Zehan Hu Chengliang Chai Bin Zhou Jijie Chai | 2013 | Cell Research2013，23，11： | 24 |
| 5 | Laparoscopic low anterior resection for rectal carcinoma:Complications and management in 132 consecutive patients显示文摘AIM:To analyze the clinical manifestations and risk fac-tors of complications in laparoscopic low anterior resection(LAR)for rectal cancer patients.METHODS:A series of 132 consecutive patients who received laparoscopic LAR for rectal cancer in our center were included.The etiology,diagnosis,treatment and prevention of rectal cancer were studied among the patients with surgery-related complications using both univariate and multivariate regression analysis.RESULTS:No conversion to open surgery was observed and 5 cases converted to hand-assisted laparoscopic operation.The overall morbidity rate was 20.5%.Complications occurred during the operation in 7 patients(5.3%),within 30 postoperative days in 24 patients(18.2%),and within 3 mo in 2 patients(1.5%).The most significant complications were anastomotic leak-age(9.1%)and anastomotic hemorrhage(5.3%).Sizeand location of tumor,pathological staging and preoperative nutrition were significant factors associated with LAR complications,while gender,age and pathological type showed no relevance.Binary logistics regression showed that the size and location of tumor,and pathological staging were independent factors of laparoscopic LAR.All the complications were treated during their onset of clinical manifestations by interventional or conservative therapy.CONCLUSION:Anastomotic leakage is a major com-plication in laparoscopic LAR.The complications may be associated with tumor size and site,and pathological stage.Interventional therapies are of value in the management of laparoscopic LAR complications. | Qian-Lin Zhu,Bo Feng,Ai-Guo Lu,Ming-Liang Wang,Wei-Guo Hu,Jian-Wen Li,Zhi-Hai Mao,Min-Hua Zheng,Department of General Surgery,Shanghai Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China Shanghai Minimally Invasive Surgery Center,Shanghai 200025,China | 2010 | World Journal of Gastroenterology2010，16，36： | 23 |
| 6 | Expert consensus on the use of human serum albumin in critically ill patients显示文摘Introduction Human serum albumin(HSA)is a non-glycosylated,negatively charged,single-chain polypeptide composed of 585 amino acid residues with a relative molecular mass of 66.438 kD.It is synthesized by the liver at a rate of approximately 200 mg·kg^(-1)·day^(-1),with a half-life of 21 days,and subjected to catabolism in the muscles,liver,and kidneys at a rate of 4%per day.[1]Albumin,accounting for 60%of the total plasma protein,has various physiological functions,[2]such as maintaining 70%to 80%of effective plasma colloid osmotic pressure,coordinating vascular endothelial integrity,anti-oxidant and anti-inflammatory activities,maintaining the acidbase balance,and participating in the transport,distribution,and metabolism of a variety of endogenous and exogenous substances. | Yue-Tian Yu Jiao Liu Bo Hu Rui-Lan Wang Xiang-Hong Yang Xiu-Ling Shang Gang Wang Chang-Song Wang Bai-Ling Li Ye Gong Sheng Zhang Xin Li Lu Wang Min Shao Mei Meng Feng Zhu You Shang Qiang-Hong Xu Zhi-Xiong Wu De-Chang Chen | 2021 | Chinese Medical Journal2021，，14： | 22 |
| 7 | IFN-γ release assay: a diagnostic assistance tool of tuberculin skin test in pediatric tuberculosis in China显示文摘Mycobacterium 的背景迅速的诊断肺结核(鱼雷快艇) 感染是在肺结核控制和消除的必要的步。然而,精确地诊断小儿科的肺结核(TB ) 经常是困难的。因为,结核菌素测试( TST )可以有低特性与在 Mycobacterium bovis 杆菌 Calmette-Guerin ( BCG )和另外的 mycobacteria 在场的抗原跨反应,特别在有主要 种牛痘BCG 的 藏匿population.Early 的 antigenic 目标 6-kDa 的中国,蛋白质( ESAT-6 )和文化过滤蛋白质 10 ( CFP-10 ),作为导致 interferon-gamma (IFN-)的合适的抗原站出来 secreting ,对感染的 T-cell-mediated 有免疫力的反应。当时,考虑了IFN-版本试金( TSPOT )的更高的费用和复杂性,我们试图在小儿科的肺结核的临床的诊断评估 TSPOT 和 TST 测试并且建立对 China.Methods 合适的一个诊断过程试金的敏感和特性与另外的疾病与活跃肺结核和 51 个 nontuberculous 孩子在总数 74 孩子被评估,然后结果与 TST.Logistic 回归模型相比习惯于 identif 独立变量包括了性别,年龄,出生地方,种痘历史,有 TSPOT 的敏感是的活跃 TB patient.Results 的靠近的合同比在活跃 TB 孩子与或没有 BCG 种痘,以及在有证实文化的 TB 的孩子的 TST 高。但是差别不统计上是重要的。TSPOT 和 TST 的联合结果改进了敏感到 94.6% 。TST 和 TSPOT 的同意是低的(77.0% , k=0.203 ) 在活跃 TB 病人。在在 TSPOT 和 TST 测试之间的特性的差别是统计上重要的(94.1%vs.70.6% , P=0.006 ) 。没有优先的 BCG 种痘历史的病人的二测试的特性是类似的(80.0%vs.60.0%) 。在在 BCG 的二测试结果之间的词语索引种牛痘题目是低的(71.7% , k=0.063 ) 。为 TSPOT,任何一个都显著地没与积极结果包括的风险因素被联系。为 TST, BCG 种痘(OR:1.78;95% CI:1.30-2.00 ) 尽管 IFN- 版本试金有相对高的敏感和特性,显著地与积极 results.Conclusions 被联系,我们应该也考虑这测试的更高的费用和复杂性。因此,当有否定 TST 的一个怀疑的病人结果时, TSPOT 能在情形被用作 TST 的互补工具,或由 BCG 种痘引起了排除积极 TST 结果。 | SUN Lin YAN Hui-min HU Ying-hui JIAO Wei-wei GU Yi XIAO Jing LI Hui-min JIAO An-xia GUO Ya-jie SHEN A-dong | 2010 | Chinese Medical Journal2010，，20： | 21 |
| 8 | GRAIN INCOMPLETE FILLING 2 regulates grain filling and starch synthesis during rice caryopsis development显示文摘Rice grain filling determines grain weight,final yield and grain quality.Here,a rice defective grain filling mutant,gif2,was identified.Grains of gif2 showed a slower filling rate and a significant lower final grain weight and yield compared to wild-type.The starch content in gif2 was noticeably decreased and its physicochemical properties were also altered.Moreover,gif2 endosperm cells showed obvious defects in compound granule formation.Positional cloning identified GIF2 to encode an ADP-glucose pyrophosphorylase(AGP) large subunit,AGPL2;consequently,AGP enzyme activity in gif2 endosperms was remarkably decreased.GIF2 is mainly expressed in developing grains and the coded protein localizes in the cytosol.Yeast two hybrid assay showed that GIF2 interacted with AGP small subunits Os AGPS1,Os AGPS2a and Os AGPS2 b.Transcript levels for granule-bound starch synthase,starch synthase,starch branching enzyme and starch debranching enzyme were distinctly elevated in gif2 grains.In addition,the level of nucleotide diversity of the GIF2 locus was extremely low in both cultivated and wild rice.All of these results suggest that GIF2 plays important roles in the regulation of grain filling and starch biosynthesis during caryopsis development,and that it has been preserved during selection throughout domestication of modern rice. | Xiangjin Wei Guiai Jiao Haiyan Lin Zhonghua Sheng Gaoneng Shao Lihong Xie Shaoqing Tang Qingguo Xu Peisong Hu | 2017 | Journal of Integrative Plant Biology2017，59，2： | 20 |
| 9 | Characteristics and triggering mechanism of Xinmo landslide on 24 June 2017 in Sichuan, China显示文摘At 5: 39 AM on 24 June 2017, a huge landslide-debris avalanche occurred on Fugui Mountain at Xinmo village, Diexi town, Maoxian county, Sichuan province, China. The debris blocked the Songpinggou River for about 2 km, resulting in a heavy loss of both human lives and properties(10 deaths, 3 injuries, 73 missing, and 103 houses completely destroyed). The objectives of this paper are to understand the overall process and triggering factors of this landslide and to explore the affecting factors for its long term evolution before failure. Post event surveys were carried out the day after the landslide occurrence. Information was gathered from literature and on-site investigation and measurement. Topography, landforms, lithology, geological setting, earthquake history, meteorological and hydrological data of the area were analysed. Aerial photographs and other remote sensing information were used for evaluation and discussion. Eye witnesses also provided a lot of helpful information for us to understand the process of initiation, development and deposition. The depositional characteristics of the moving material as well as the traces of the movement,the structural features of the main scarp and the seismic waves induced by the slide are presented and discussed in detail in this paper. The results show that the mechanism of the landslide is a sudden rupture of the main block caused by the instability of a secondary block at a higher position. After the initiation, the failed rock mass at higher position overloaded the main block at the lower elevation and collapsed in tandem. Fragmentation of the rock mass occurred later, thus forming a debris avalanche with high mobility. This landslide case indicates that such seismic events could influence geological hazards for over 80 years and this study provides reference to the long term susceptibility and risk assessment of secondary geological hazards from earthquake. | SU Li-jun HU Kai-heng ZHANG Wei-feng WANG Jiao LEI Yu ZHANG Chong-lei CUI Peng Alessandro PASUTO ZHENG Quan-hong | 2017 | Journal of Mountain Science2017，14，9： | 20 |
| 10 | Lung transplantation as therapeutic option in acute respiratory distress syndrome for coronavirus disease 2019-related pulmonary fibrosis显示文摘Background:Critical patients with the coronavirus disease 2019(COVID-19),even those whose nucleic acid test results had turned negative and those receiving maximal medical support,have been noted to progress to irreversible fatal respiratory failure.Lung transplantation(LT)as the sole therapy for end-stage pulmonary fibrosis related to acute respiratory distress syndrome has been considered as the ultimate rescue therapy for these patients.Methods:From February 10 to March 10,2020,three male patients were urgently assessed and listed for transplantation.After conducting a full ethical review and after obtaining assent from the family of the patients,we performed three LT procedures for COVID-19 patients with illness durations of more than one month and extremely high sequential organ failure assessment scores.Results:Two of the three recipients survived post-LT and started participating in a rehabilitation program.Pearls of the LT team collaboration and perioperative logistics were summarized and continually improved.The pathological results of the explanted lungs were concordant with the critical clinical manifestation,and provided insight towards better understanding of the disease.Government health affair systems,virology detection tools,and modern communication technology all play key roles towards the survival of the patients and their rehabilitation.Conclusions:LT can be performed in end-stage patients with respiratory failure due to COVID-19-related pulmonary fibrosis.If confirmed positive-turned-negative virology status without organ dysfunction that could contraindicate LT,LT provided the final option for these patients to avoid certain death,with proper protection of transplant surgeons and medical staffs.By ensuring instant seamless care for both patients and medical teams,the goal of reducing the mortality rate and salvaging the lives of patients with COVID-19 can be attained. | Jing-Yu Chen Kun Qiao Feng Liu Bo Wu Xin Xu Guo-Qing Jiao Rong-Guo Lu Hui-Xing Li Jin Zhao Jian Huang Yi Yang Xiao-Jie Lu Jia-Shu Li Shu-Yun Jiang Da-Peng Wang Chun-Xiao Hu Gui-Long Wang Dong-Xiao Huang Guo-Hui Jiao Dong Wei Shu-Gao Ye Jian-An Huang Li Zhou Xiao-Qin Zhang Jian-Xing He | 2020 | Chinese Medical Journal2020，，12： | 19 |
| 11 | Using Hourly Measurements to Explore the Role of Secondary Inorganic Aerosol in PM_(2.5)during Haze and Fog in Hangzhou, China显示文摘This paper explores the role of the secondary inorganic aerosol(SIA) species ammonium, NH+4, nitrate, NO-3, and sulfate,SO2-4, during haze and fog events using hourly mass concentrations of PM2.5measured at a suburban site in Hangzhou,China. A total of 546 samples were collected between 1 April and 8 May 2012. The samples were analyzed and classified as clear, haze or fog depending on visibility and relative humidity(RH). The contribution of SIA species to PM2.5mass increased to ~50% during haze and fog. The mass contribution of nitrate to PM2.5increased from 11% during clear to 20%during haze episodes. Nitrate mass exceeded sulfate mass during haze, while near equal concentrations were observed during fog episodes. The role of RH on the correlation between concentrations of SIA and visibility was examined, with optimal correlation at 60%–70% RH. The total acidity during clear, haze and fog periods was 42.38, 48.38 and 45.51 nmol m-3,respectively, indicating that sulfate, nitrate and chloride were not neutralized by ammonium during any period. The nitrate to sulfate molar ratio, as a function of the ammonium to sulfate molar ratio, indicated that nitrate formation during fog started at a higher ammonium to sulfate molar ratio compared to clear and haze periods. During haze and fog, the nitrate oxidation ratio increased by a factor of 1.6–1.7, while the sulfur oxidation ratio increased by a factor of 1.2–1.5, indicating that both gaseous NO2 and SO2were involved in the reduced visibility. | Roeland Cornelis JANSEN SHI Yang CHEN Jianmin HU YunJie XU Chang HONG Shengmao LI Jiao ZHANG Min | 2014 | Advances in Atmospheric Sciences2014，31，6： | 15 |
| 12 | Precise orbit determination of a maneuvered GEO satellite using CAPS ranging data显示文摘Wheel-off-loadings and orbital maneuvers of the GEO satellite result in additional accelerations to the satellite itself. Complex and difficult to model, these time varying accelerations are an important error source of precise orbit determination (POD). In most POD practices, only non-maneuver orbital arcs are treated. However, for some applications such as satellite navigation RDSS services, uninterrupted orbital ephemeris is demanded, requiring the development of POD strategies to be processed both during and after an orbital maneuver. We in this paper study the POD for a maneuvered GEO satellite, using high precision and high sampling rate ranging data obtained with Chinese Area Positioning System (CAPS). The strategy of long arc POD including maneuver arcs is studied by using telemetry data to model the maneuver thrust process. Combining the thrust and other orbital perturbations, a long arc of 6 days’ CAPS ranging data is analyzed. If the telemetry data are not available or contain significant errors, attempts are made to estimate thrusting parameters using CAPS ranging data in the POD as an alternative to properly account for the maneuver. Two strategies achieve reasonably good data fitting level in the tested arc with the maximal position difference being about 20 m. | HUANG Yong HU XiaoGong HUANG Cheng YANG QiangWen JIAO WenHai | 2009 | Science China(Physics,Mechanics & Astronomy)2009，52，3： | 13 |
| 13 | COUPLED NUMERICAL SIMULATION ON COLD ROLLER'S TEMPERATURE FIFLD-PHASE TRANSFORMATION - STRESS FIELD DURING ITS QUENCHING PSOCESS显示文摘Complicated changns occur inside the steel parts during quenching process. The abruptly changed boundary conditions make the temperature field,micro - structure and stress field change dramatically in very short time, and these variables take a contact interactions in the whole process. In this paper, a three dimensional non - linear mathematical model for queeching process has been founded and the numerical simulation on temperature field,microstructre and stress field has been realized.In the FEM analysis, the incremental iteration method is used to deal with such complicated nonlinear as boundary nonlinear, physical property nonlinear,transformation nonlinear etc.The effect of stress on transformation kinetics has been considered in the calculation of microstructure. In the stress field anal- ysis,a thermo- elasto - plastic model has been founded, which considers such factors as transforma- tion strain,transformation plastic strain, themal strain and the effect of temperature and transforma- tion on mechanical propertier etc. The transient temperature field, microstructure distribution and stress field of the roller on any time can be displayed vividly,and the cooling curve and the changes of stress on any position can also be given. | J. F. Gu J. S. Pan M. J. Hu and F. F. Shen (School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200030, China) | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，1： | 13 |
| 14 | MAJOR-EFFICIENT SOLUTIONS AND WEAKLY MAJOR-EFFICIENT SOLUTIONS OF MULTIOBJECTIVE PROGRAMMING显示文摘MAJOR-EFFICIENTSOLUTIONSANDWEAKLYMAJOR-EFFICIENTSOLUTIONSOFMULTIOBJECTIVEPROGRAMMING￥HUYUDA(Dept.ofAppl.Math.,ShanghaiJiaoTon... | HU YUDA(Dept.of Appl.Math.,Shanghai Jiao Tony Univ.,Shanghai 200030) | 1994 | Applied Mathematics(A Journal of Chinese Universities)1994，9，1： | 12 |
| 15 | Excitation and Detection of Shear Horizontal Waves with Electromagnetic Acoustic Transducers for Nondestructive Testing of Plates显示文摘The fundamental shear horizontal(SH0) wave has several unique features that are attractive for long-range nondestructive testing(NDT). By a careful design of the geometric configuration, electromagnetic acoustic transducers(EMATs) have the capability to generate a wide range of guided wave modes, such as Lamb waves and shear-horizontal(SH) waves in plates. However, the performance of EMATs is influenced by their parameters. To evaluate the performance of periodic permanent magnet(PPM) EMATs, a distributed-line-source model is developed to calculate the angular acoustic field cross-section in the far-field. Numerical analysis is conducted to investigate the performance of such EMATs with different geometric parameters, such as period and number of magnet arrays, and inner and outer coil widths. Such parameters have a great influence on the directivity of the generated SH0 waves that arises mainly in the amplitude and width of both main and side lobes. According to the numerical analysis, these parameters are optimized to obtain better directivity. Optimized PPM EMATs are designed and used for NDT of strip plates. Experimental results show that the lateral boundary of the strip plate has no perceivable influence on SH0-wave propagation, thus validating their used in NDT. The proposed model predicts the radiation pattern of PPM EMATs, and can be used for their parameter optimization. | MA Qingzeng JIAO Jingpin HU Ping ZHONG Xi WU Bin HE Cunfu | 2014 | Chinese Journal of Mechanical Engineering2014，27，2： | 12 |
| 16 | Carbon pools and fluxes in the China Seas and adjacent oceans显示文摘The China Seas include the South China Sea, East China Sea, Yellow Sea, and Bohai Sea. Located off the Northwestern Pacific margin, covering 4700000 km^2 from tropical to northern temperate zones, and including a variety of continental margins/basins and depths, the China Seas provide typical cases for carbon budget studies. The South China Sea being a deep basin and part of the Western Pacific Warm Pool is characterized by oceanic features; the East China Sea with a wide continental shelf, enormous terrestrial discharges and open margins to the West Pacific, is featured by strong cross-shelf materials transport; the Yellow Sea is featured by the confluence of cold and warm waters; and the Bohai Sea is a shallow semiclosed gulf with strong impacts of human activities. Three large rivers, the Yangtze River, Yellow River, and Pearl River, flow into the East China Sea, the Bohai Sea, and the South China Sea, respectively. The Kuroshio Current at the outer margin of the Chinese continental shelf is one of the two major western boundary currents of the world oceans and its strength and position directly affect the regional climate of China. These characteristics make the China Seas a typical case of marginal seas to study carbon storage and fluxes. This paper systematically analyzes the literature data on the carbon pools and fluxes of the Bohai Sea,Yellow Sea, East China Sea, and South China Sea, including different interfaces(land-sea, sea-air, sediment-water, and marginal sea-open ocean) and different ecosystems(mangroves, wetland, seagrass beds, macroalgae mariculture, coral reefs, euphotic zones, and water column). Among the four seas, the Bohai Sea and South China Sea are acting as CO_2 sources, releasing about0.22 and 13.86–33.60 Tg C yr^(-1) into the atmosphere, respectively, whereas the Yellow Sea and East China Sea are acting as carbon sinks, absorbing about 1.15 and 6.92–23.30 Tg C yr^(-1) of atmospheric CO_2, respectively. Overall, if only the CO_2 exchange at the sea-air interface is considered, the Chinese marginal seas appear to be a source of atmospheric CO_2, with a net release of 6.01–9.33 Tg C yr^(-1), mainly from the inputs of rivers and adjacent oceans. The riverine dissolved inorganic carbon (DIC) input into the Bohai Sea and Yellow Sea, East China Sea, and South China Sea are 5.04, 14.60, and 40.14 Tg C yr^(-1),respectively. The DIC input from adjacent oceans is as high as 144.81 Tg C yr^(-1), significantly exceeding the carbon released from the seas to the atmosphere. In terms of output, the depositional fluxes of organic carbon in the Bohai Sea, Yellow Sea, East China Sea, and South China Sea are 2.00, 3.60, 7.40, and 5.92 Tg C yr^(-1), respectively. The fluxes of organic carbon from the East China Sea and South China Sea to the adjacent oceans are 15.25–36.70 and 43.93 Tg C yr^(-1), respectively. The annual carbon storage of mangroves, wetlands, and seagrass in Chinese coastal waters is 0.36–1.75 Tg C yr^(-1), with a dissolved organic carbon(DOC) output from seagrass beds of up to 0.59 Tg C yr^(-1). Removable organic carbon flux by Chinese macroalgae mariculture account for 0.68 Tg C yr^(-1) and the associated POC depositional and DOC releasing fluxes are 0.14 and 0.82 Tg C yr^(-1), respectively. Thus, in total, the annual output of organic carbon, which is mainly DOC, in the China Seas is 81.72–104.56 Tg C yr^(-1). The DOC efflux from the East China Sea to the adjacent oceans is 15.00–35.00 Tg C yr^(-1). The DOC efflux from the South China Sea is 31.39 Tg C yr^(-1). Although the marginal China Seas seem to be a source of atmospheric CO_2 based on the CO_2 flux at the sea-air interface, the combined effects of the riverine input in the area, oceanic input, depositional export,and microbial carbon pump(DOC conversion and output) indicate that the China Seas represent an important carbon storage area. | Nianzhi JIAO Yantao LIANG Yongyu ZHANG Jihua LIU Yao ZHANG Rui ZHANG Meixun ZHAO Minhan DAI Weidong ZHAI Kunshan GAO Jinming SONG Dongliang YUAN Chao LI Guanghui LIN Xiaoping HUANG Hongqiang YAN Limin HU Zenghu ZHANG Long WANG Chunjie CAO Yawei LUO Tingwei LUO Nannan WANG Hongyue DANG Dongxiao WANG Si ZHANG | 2018 | Science China Earth Sciences2018，61，11： | 12 |
| 17 | GBSS-BINDING PROTEIN,encoding a CBM48 domain-containing protein,affects rice quality and yield^∞显示文摘The percentage of amylose in the endosperm of rice(Oryza sativa)largely determines grain cooking and eating qualities.Granule-bound starch synthase I(GBSSI)and GBSSII are responsible for amylose biosynthesis in the endosperm and leaf,respectively.Here,we identified OsGBP,a rice GBSS-binding protein that interacted with GBSSI and GBSSII in vitro and in vivo.The total starch and amylose contents in osgbp mutants were significantly lower than those of wild type in leaves and grains,resulting in reduced grain weight and quality.The carbohydrate-binding module 48(CBM48)domain present in the C-terminus of OsGBP is crucial for OsGBP binding to starch.In the osgbp mutant,the extent of GBSSI and GBSSII binding to starch in the leaf and endospermwas significantly lower than wild type.Our data suggest that OsGBP plays an important role in leaf and endosperm starch biosynthesis by mediating the binding of GBSS proteins to developing starch granules.This elucidation of the function of OsGBP enhances our understanding of the molecular basis of starch biosyn-thesis in rice and contributes information that can be potentially used for the genetic improvement of yield and grain quality. | Wei Wang Xiangjin Wei Guiai Jiao Wenqiang Chen Yawen Wu Zhonghua Sheng Shikai Hu Lihong Xie Jiayu Wang Shaoqing Tang Peisong Hu | 2020 | Journal of Integrative Plant Biology2020，62，7： | 12 |
| 18 | Targeted mutagenesis of POLYAMINE OXIDASE 5 that negatively regulates mesocotyl elongation enables the generation of direct-seeding rice with improved grain yield显示文摘Under conditions of labor or resource scarcity,direct seeding,rather than transplantation,is the preferred mode of rice(Oryza sativa)cultivation.This approach requires varieties that exhibit uniform seedling emergence.Mesocotyl elongation(ME),the main driver of rapid emergence of rice seedlings from soil,is enhanced by darkness and inhibited by light.Plant polyamine oxidases(PAOs)oxidize polyamines(PAs)and release H2O2,Here,we established that OsPAO5 expression in rice seedlings is increased in the presence of light and inhibited by darkness.To determine its role in ME,we created OsPAO5 mutants using CRISPR/Cas9.Compared with the wild type,pao5 mutants had longer mesocotyls,released less H2O2,and synthesized more ethylene.The mutant seedlings emerged at a higher and more uniform rate,indicating their potential for use in direct seeding.Nucleotide polymorphism analysis revealed that an SNP(PAO5-578G/A)located 578 bp upstream of the OsPAO5 start codon alters its expression,and was selected during rice mesocotyl domestication.The PAO5-578G genotype conferring a long mesocotyl mainly exists in wild rice,most Aus accessions,and some Geng(Japonica)accessions.Intriguingly,knocking out OsPAO5 can remarkably increase the grain weight,grain number,and yield potential.In summary,we developed a novel strategy to obtain elite rice with higher emergence vigor and yield potential,which can be conveniently and widely used to breed varieties of direct-seeding rice. | Yusong Lv Gaoneng Shao Guiai Jiao Zhonghua Sheng Lihong Xie Shikai Hu Shaoqing Tang Xiangjin Wei Peisong Hu | 2021 | Molecular Plant2021，14，2： | 12 |
| 19 | LEAFY HEAD2,which encodes a putative RNA-binding protein,regulates shoot development of rice显示文摘在植物的开发期间,更高的植物连续地在常规空间、时间的模式形成新叶子。有反常的叶的异种发展模式不仅提供大卓见进理解植物建筑学的规章的机制,而且充实方法到它庄稼产量能被改进的修正。这里,我们报导了米饭 leafy-head2 (lhd2 ) 的描述展览弄短的异种阶段的 plastochron,侏儒症,减少的 tiller 数字,和失败转移从对繁殖生长植物。而减少的 tiller 数字是 tiller 芽长出的抑制的后果,解剖、组织学的学习表明在 lhd2 的叶子的快速的出现从叶 primordia 的快速的开始被结果。分子、基因的分析证明 LHD2 与 67% 类似编码通常认为的 RNA 有约束力的蛋白质到玉米 TE1。染色体规模表示侧面在之间的比较野类型并且 lhd2 植物建议 LHD2 可以通过 KNOX 和荷尔蒙相关的基因调整米饭射击开发。LHD2 变化和 LHD2 的保存表示模式引起的类似的显型在在草控制时间的叶开始显示了保存机制。 | Guo Sheng Xiong Xing Ming Hu Yong Qing Jiao Yan Chun Yu Cheng Cai Chu Jia Yang Li Qian Qian Yong Hong Wang | 2006 | Cell Research2006，16，3： | 11 |
| 20 | Mitochondrial pathway mediated by reactive oxygen species involvement in α-hederin-induced apoptosis in hepatocellular carcinoma cells显示文摘AIM To investigate the antitumor activity of α-hederin in hepatocellular carcinoma(HCC) cells and its underlying mechanisms in vitro and in vivo.METHODS SMMC-7721, Hep G-2 and Huh-7 HCC cells were cultured in vitro and treated with α-hederin(0, 5 μmol/L, 10 μmol/L, 15 μmol/L, 20 μmol/L, 25 μmol/L, 30 μmol/L, 35 μmol/L, 40 μmol/L, 45 μmol/L, 50 μmol/L, 55 μmol/L, or 60 μmol/L) for 12 h, 24 h, or 36 h, and cell viability was then detected by the Cell Counting Kit-8. SMMC-7721cells were treated with 0, 5 μmol/L, 10 μmol/L, or 20 μmol/L α-hederin for 24 h with or without DL-buthionineS,R-sulfoximine(2 mmol/L) or N-acetylcysteine(5 mmol/L) pretreatment for 2 h, and additional assays were subsequently performed. Apoptosis was observed after Hoechst staining. Glutathione(GSH) and adenosine triphosphate(ATP) levels were measured using GSH and ATP Assay Kits. Intracellular reactive oxygen species(ROS) levels were determined by measuring the oxidative conversion of 2',7'-dichlorofluorescin diacetate. Disruption of the mitochondrial membrane potential was evaluated using JC-1 staining. The protein levels of Bax, Bcl-2, cleaved caspase-3, cleaved caspase-9, apoptosis-inducing factor and cytochrome C were detected by western blotting. The antitumor efficacy of α-hederin in vivo was evaluated in a xenograft tumor model.RESULTS The α-hederin treatment induced apoptosis of HCC cells. The apoptosis rates in the control, low-dose α-hederin(5 μmol/L), mid-dose α-hederin(10 μmol/L) and highdose α-hederin(20 μmol/L) groups were 0.90% ± 0.26%, 12% ± 2.0%, 21% ± 2.1% and 37% ± 3.8%, respectively(P < 0.05). The α-hederin treatment reduced intracellular GSH and ATP levels, induced ROS, disrupted the mitochondrial membrane potential, increased the protein levels of Bax, cleaved caspase-3, cleaved caspase-9, apoptosis-inducing factor and cytochrome C, and decreased Bcl-2 expression. The α-hederin treatment also inhibited xenograft tumor growth in vivo. CONCLUSION The α-hederin saponin induces apoptosis of HCC cells via the mitochondrial pathway mediated by increased intracellular ROS and may be an effective treatment for human HCC. | Jiao Li Dan-Dan Wu Ji-Xiang Zhang Jing Wang Jing-Jing Ma Xue Hu Wei-Guo Dong | 2018 | World Journal of Gastroenterology2018，24，17： | 11 |
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