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| 1 | Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors显示文摘Multipotent 神经干细胞为房间治疗保持大诺言。到导致的 pluripotent 干细胞以及成熟神经原的成纤维细胞的 reprogramming 建议可能性没有首先建立 pluripotency,把一个严重地区分的体的房间变换成一个 multipotent 状态。这里,我们证明房间从中层导出的那 Sertoli 能直接被变换成拥有神经干细胞性质的一个 multipotent 状态。导致的神经干细胞(iNSCs ) 表示多重 NSC 特定的标记,展出类似于正常 NSC 的全球基因表示侧面,并且能够自强并且区分功能的神经原进神经胶质并且 electrophysiologically。为酷氨酸 hydroxylase (TH ) 积极的导出 iNSC 的神经原污点, γ -aminobutyric 酸,和胆碱 acetyltransferase。另外, iNSCs 能熬过并且产生跟随移植进有牙齿的回转的触处。iNSCs 的产生可以为疾病建模和再生药有重要含意。 | Chao Sheng Qinyuan Zheng Jianyu Wu Zhen Xu Libin Wang Wei Li Haijiang Zhang Xiao-Yang Zhao Lei Liu Ziwei Wang Changlong Guo Hua-Jun Wu Zhonghua Liu Liu Wang Shigang He Xiu-Jie Wang Zhiguo Chen Qi Zhou | 2012 | Cell Research2012，22，1： | 31 |
| 2 | GRAIN INCOMPLETE FILLING 2 regulates grain filling and starch synthesis during rice caryopsis development显示文摘Rice grain filling determines grain weight,final yield and grain quality.Here,a rice defective grain filling mutant,gif2,was identified.Grains of gif2 showed a slower filling rate and a significant lower final grain weight and yield compared to wild-type.The starch content in gif2 was noticeably decreased and its physicochemical properties were also altered.Moreover,gif2 endosperm cells showed obvious defects in compound granule formation.Positional cloning identified GIF2 to encode an ADP-glucose pyrophosphorylase(AGP) large subunit,AGPL2;consequently,AGP enzyme activity in gif2 endosperms was remarkably decreased.GIF2 is mainly expressed in developing grains and the coded protein localizes in the cytosol.Yeast two hybrid assay showed that GIF2 interacted with AGP small subunits Os AGPS1,Os AGPS2a and Os AGPS2 b.Transcript levels for granule-bound starch synthase,starch synthase,starch branching enzyme and starch debranching enzyme were distinctly elevated in gif2 grains.In addition,the level of nucleotide diversity of the GIF2 locus was extremely low in both cultivated and wild rice.All of these results suggest that GIF2 plays important roles in the regulation of grain filling and starch biosynthesis during caryopsis development,and that it has been preserved during selection throughout domestication of modern rice. | Xiangjin Wei Guiai Jiao Haiyan Lin Zhonghua Sheng Gaoneng Shao Lihong Xie Shaoqing Tang Qingguo Xu Peisong Hu | 2017 | Journal of Integrative Plant Biology2017，59，2： | 20 |
| 3 | GBSS-BINDING PROTEIN,encoding a CBM48 domain-containing protein,affects rice quality and yield^∞显示文摘The percentage of amylose in the endosperm of rice(Oryza sativa)largely determines grain cooking and eating qualities.Granule-bound starch synthase I(GBSSI)and GBSSII are responsible for amylose biosynthesis in the endosperm and leaf,respectively.Here,we identified OsGBP,a rice GBSS-binding protein that interacted with GBSSI and GBSSII in vitro and in vivo.The total starch and amylose contents in osgbp mutants were significantly lower than those of wild type in leaves and grains,resulting in reduced grain weight and quality.The carbohydrate-binding module 48(CBM48)domain present in the C-terminus of OsGBP is crucial for OsGBP binding to starch.In the osgbp mutant,the extent of GBSSI and GBSSII binding to starch in the leaf and endospermwas significantly lower than wild type.Our data suggest that OsGBP plays an important role in leaf and endosperm starch biosynthesis by mediating the binding of GBSS proteins to developing starch granules.This elucidation of the function of OsGBP enhances our understanding of the molecular basis of starch biosyn-thesis in rice and contributes information that can be potentially used for the genetic improvement of yield and grain quality. | Wei Wang Xiangjin Wei Guiai Jiao Wenqiang Chen Yawen Wu Zhonghua Sheng Shikai Hu Lihong Xie Jiayu Wang Shaoqing Tang Peisong Hu | 2020 | Journal of Integrative Plant Biology2020，62，7： | 12 |
| 4 | Targeted mutagenesis of POLYAMINE OXIDASE 5 that negatively regulates mesocotyl elongation enables the generation of direct-seeding rice with improved grain yield显示文摘Under conditions of labor or resource scarcity,direct seeding,rather than transplantation,is the preferred mode of rice(Oryza sativa)cultivation.This approach requires varieties that exhibit uniform seedling emergence.Mesocotyl elongation(ME),the main driver of rapid emergence of rice seedlings from soil,is enhanced by darkness and inhibited by light.Plant polyamine oxidases(PAOs)oxidize polyamines(PAs)and release H2O2,Here,we established that OsPAO5 expression in rice seedlings is increased in the presence of light and inhibited by darkness.To determine its role in ME,we created OsPAO5 mutants using CRISPR/Cas9.Compared with the wild type,pao5 mutants had longer mesocotyls,released less H2O2,and synthesized more ethylene.The mutant seedlings emerged at a higher and more uniform rate,indicating their potential for use in direct seeding.Nucleotide polymorphism analysis revealed that an SNP(PAO5-578G/A)located 578 bp upstream of the OsPAO5 start codon alters its expression,and was selected during rice mesocotyl domestication.The PAO5-578G genotype conferring a long mesocotyl mainly exists in wild rice,most Aus accessions,and some Geng(Japonica)accessions.Intriguingly,knocking out OsPAO5 can remarkably increase the grain weight,grain number,and yield potential.In summary,we developed a novel strategy to obtain elite rice with higher emergence vigor and yield potential,which can be conveniently and widely used to breed varieties of direct-seeding rice. | Yusong Lv Gaoneng Shao Guiai Jiao Zhonghua Sheng Lihong Xie Shikai Hu Shaoqing Tang Xiangjin Wei Peisong Hu | 2021 | Molecular Plant2021，14，2： | 12 |
| 5 | Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation显示文摘Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease 9(CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T_0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1(type 1, with an adenine insertion) and cr-isa1-2(type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding. | CHAO Shufen CAI Yicong FENG Baobing JIAO Guiai SHENG Zhonghua LUO Ju TANG Shaoqing WANG Jianlong HU Peisong WEI Xiangjin | 2019 | Rice science2019，26，2： | 10 |
| 6 | OsVP1 activates Sdr4 expression to control rice seed dormancy via the ABA signaling pathway显示文摘Pre-harvest sprouting(PHS)is a disadvantageous trait in cereal production worldwide,causing large economic losses each year.Its regulation mechanism is still unclear.We generated the Oryza sativa Viviparous1(OsVP1)mutant using gene editing technique,which shows increased PHS compared with that of the wild type Nipponbare.OsVP1 is localized mainly in the nucleus and expressed in various tissues and organs.Expression of Seed dormancy 4(Sdr4),a key gene controlling PHS,was sharply reduced in OsVP1 mutants.OsVP1 bound to the specific motif CACCTG in the promoter of Sdr4 and activated its expression in rice protoplasts.Overexpression of Sdr4 reduced the high seed germination rate of OsVP1 mutant cr-osvp1-1,showing that Sdr4 acts as a downstream target of OsVP1.Both OsVP1 and Sdr4 loss-of-function mutants were insensitive to exogenous ABA and employed the ABA signaling pathway in regulating seed dormancy.These findings shed light on the control of seed dormancy aimed at preventing PHS in rice. | Wenqiang Chen Wei Wang Yusong Lyu Yawen Wu Pingliang Huang Shikai Hu Xiangjin Wei Guiai Jiao Zhonghua Sheng Shaoqing Tang Gaoneng Shao Ju Luo | 2021 | The Crop Journal2021，9，1： | 5 |
| 7 | Generation of Tripotent Neural Progenitor Cells from Rat Embryonic Stem Cells显示文摘老鼠是为药理学和生理的研究的一个珍贵模型。胚胎的茎(物件) 房间衬里的 Germline 能干的老鼠成功地被建立了,维持物件细胞的自我更新的、无差别的状态的分子的网络也好揭开。然而,很少对区别策略和这些真老鼠 pluripotent 干细胞怎么产生特定的房间类型的内在的机制被知道。这研究的目的是调查物件房间的神经区别能力。借助于一个修改过程基于 激活mitogen 的蛋白质 kinase ( MAPK )和肝糖 synthase kinase 的以前的出版物联合 3 ( GSK3 )禁止者(二个禁止者, 2i )与喂调节中等,我们成功地从物件房间获得了高质量的老鼠胚胎植物或动物身体(美国南北战争时南军士兵)然后区分了他们到 tripotent 神经祖先。这些物件导出房间的神经祖先房间(rNPCs ) 能够自我更新并且产生所有三个神经的系,包括的星形细胞, oligodendrocytes,和神经原。而且,这些导出房间的神经原染色了的物件为 -aminobutyric 酸(伽马氨基丁酸) 和酷氨酸 hydroxylase (TH ) 积极。在摘要,我们为区分物件房间到 tripotent 开发一个试验性的系统神经祖先,它可以为学习象 Parkinson 的疾病和老鼠神经系统的开发那样的许多 neurodegenerative 混乱的致病为药理学测试和一个珍贵平台提供一个强大的工具。 | Zhenkun Wang Chao Sheng Tianda Li Fei Teng Lisi Sang Fenglin Cao Ziwei Wang Wanwan Zhu Wei Li Xiaoyang Zhao Zhonghua Liu Liu Wang Qi Zhou | 2012 | Journal of Genetics and Genomics2012，39，12： | 2 |
| 8 | CDE4 encodes a pentatricopeptide repeat protein involved in chloroplast RNA splicing and affects chloroplast development under low-temperature conditions in rice显示文摘Pentatricopeptide repeat(PPR)proteins play important roles in the post-transcriptional modification of organellar RNAs in plants.However,the function of most PPR proteins remains unknown.Here,we characterized the rice(Oryza sativa L.)chlorophyll deficient 4(cde4)mutant which exhibits an albino phenotype during early leaf development,with decreased chlorophyll contents and abnormal chloroplasts at low-temperature(20℃).Positional cloning revealed that CDE4 encodes a P-type PPR protein localized in chloroplasts.In the cde4 mutant,plastid-encoded polymerase(PEP)-dependent transcript levels were significantly reduced,but transcript levels of nuclear-encoded genes were increased compared to wild-type plants at 20℃.CDE4 directly binds to the transcripts of the chloroplast genes rpl2,ndhA,and ndhB.Intron splicing of these transcripts was defective in the cde4 mutant at 20℃,but was normal at 32℃.Moreover,CDE4 interacts with the guanylate kinase VIRESCENT 2(V2);overexpression of V2 enhanced CDE4 protein stability,thereby rescuing the cde4 phenotype at 20℃.Our results suggest that CDE4 participates in plastid RNA splicing and plays an important role in rice chloroplast development under lowtemperature conditions. | Xinyong Liu Xichun Zhang Ruijie Cao Guiai Jiao Shikai Hu Gaoneng Shao Zhonghua Sheng Lihong Xie Shaoqing Tang Xiangjin Wei Peisong Hu | 2021 | Journal of Integrative Plant Biology2021，63，10： | 2 |
| 9 | Editing of Rice Endosperm Plastidial Phosphorylase Gene OsPho1 Advances Its Function in Starch Synthesis显示文摘OsPho1 in Zhonghua 11(ZH11)was edited using the clustered regularly interspaced short palindromic repeatsassociated endonuclease 9(CRISPR/Cas9)system.Two homozygous T1 mutants(cr-pho1-34 and cr-pho1-37)displayed a chalky endosperm with a white core,which significantly decreased 1000-grain weight.In addition,many rounded starch granules and abnormal amyloplasts were present in the central region of mutant endosperm cells with increased amylose and lipid contents,decreased total protein content,and altered physicochemical properties of starch.The OsPho1 protein is localized in chloroplasts,and quantitative real-time PCR(qRT-PCR)andβ-glucuronidase(GUS)staining indicated that OsPho1 was highly expressed in seeds at 5 d after fertilization(DAF).OsPho1 mutations displayed close relationships with plastidial phosphoglucomutase and ADPGlc pyrophosphorylase based onα-D-glucose-1P at different temperatures.Moreover,the expressions of starch metabolismrelated genes were also altered in the mutant,and the overexpression of OsPho1 may cause grain chalkiness. | LIU Song SHAO Gaoneng JIAO Guiai ZHU Maodi WU Jiamin CAO Ruijie CHEN Yujuan XIE Lihong SHENG Zhonghua TANG Shaoqing | 2021 | Rice science2021，28，3： | 1 |
| 10 | Overview of Medici- nal Functions of Tea Extracts 显示文摘 | Liu Zhonghua Zhang Sheng HuangJianan | 2002 | Natural Products and Human Health2002，，1： | 1 |
| 11 | Beam test of a full-length prototype of the BES 显示文摘 | QIN Zhonghua CHEN Yuanbo SHENG Hua yi | 2007 | Nuclear Instruments and Methods in Physics Research A2007，571，3： | 1 |
| 12 | Characteristicsand origin of underpressure system in the Shiwu fault depression, south-east Songliao Basin, China显示文摘 | SHENG H Middleton ZHONGHUA T | 2000 | Ba- sin Research2000，12，： | 1 |
| 13 | Expert consensus on the prevention and treatment of substance use and addictive behaviour-related disorders during the COVID-19 pandemic显示文摘In early 2020,the COVID-19 outbreak complicated the diagnosis,treatm ent and rehabilitation of patients with substance use disorders and increased the risks of substance abuse and addictive behaviours,such as online gaming disorders,in the general public.Substance use disorder is a chronic recurrent brain disease characterised by strong cravings,high recurrence rates,and a high proportion of comorbidity of mental and physical disorders.1 Therefore,regular long-term therapeutic interventions are critical to preventing dm g relapses while maintaining withdrawal. | Jiang Du Ni Fan Min Zhao Wei Hao Tieqiao Liu Lin Lu Jie Shi Haifeng Jiang Na Zhong Xiaochang Lan Shichao Xu Hongxian Chen Xiaojun Xiang Xuyi Wang Hongqiang Sun Bing Li Yu-Ping Ning Jing Li Wanjun Guo Yajuan Niu Lixia Sheng Yi Li Xuebin Liu Xuhui Zhou Mincai Qian Wenhua Zhou Ruiling Zhang Hongxing Hu Yan Xia Zhonghua Su Ruimin Zhang Mei Yang Fen Liu Wei Yuan | 2020 | General Psychiatry2020，33，4： | 1 |
| 14 | Integrated transcriptome, small RNA, and degradome analysis to elucidate the regulation of rice seedling mesocotyl development during the passage from darkness to light显示文摘The mesocotyl,a structure located between the basal part of the seminal root and the coleoptile node of seedlings,contributes to pushing the shoot tip through the soil surface,a function that is essential for the uniform emergence of direct-seeded rice.Its elongation is inhibited by light and induced in darkness.This investigation of an indica rice(P25)with vigorous mesocotyl elongation was aimed at identifying the'omics'basis of its lightinduced growth inhibition.A transcriptomic comparison between mesocotyl tissues that had developed in the dark and then been exposed to light identified many differentially expressed genes(DEGs)and differentially abundant micro RNAs(mi RNAs).Degradome sequencing analysis revealed 27 negative mi RNA-target pairs.A co-expression regulatory network was constructed based on the mi RNAs,their corresponding targets,and DEGs with a common Gene Ontology term.It suggested that auxin and light,probably antagonistically,affect mesocotyl elongation by regulating polyamine oxidase activity. | Yusong Lyu Xiangjin Wei Min Zhong Shipeng Niu Shakeel Ahmad Gaoneng Shao Guiai Jiao Zhonghua Sheng Lihong Xie Shikai Hu Yawen Wu Shaoqing Tang Peisong Hu | 2020 | The Crop Journal2020，8，6： | 0 |
| 15 | Approaching the binding between Cu(Ⅱ) and aerobic granules by a modified titration and p-XRF显示文摘 | Hongwei LUO Longfei WANG Zhonghua TONG Hanqing YU Guoping SHENG | 2016 | Frontiers of Environmental Science & Engineering2016，10，2： | 0 |
| 16 | OPAQUE3, encoding a transmembrane bZIP transcription factor, regulates endosperm storage protein and starch biosynthesis in rice显示文摘Starch and storage proteins are the main components of rice(Oryza sativa L.)grains.Despite their importance,the molecular regulatory mechanisms of storage protein and starch biosynthesis remain largely elusive.Here,we identified a rice opaque endosperm mutant,opaque3(o3),that overaccumulates 57-kDa proglutelins and has significantly lower protein and starch contents than the wild type.The o3 mutant also has abnormal protein body structures and compound starch grains in its endosperm cells.OPAQUE3(O3)encodes a transmembrane basic leucine zipper(bZIP)transcription factor(OsbZIP60)and is localized in the endoplasmic reticulum(ER)and the nucleus,but it is localized mostly in the nucleus under ER stress.We demonstrated that O3 could activate the expression of several starch synthesis-related genes(GBSSI,AGPL2,SBEI,and ISA2)and storage protein synthesis-related genes(OsGluA2,Prol14,and Glb1).O3 also plays an important role in protein processing and export in the ER by directly binding to the promoters and activating the expression of OsBIP1 and PDIL1-1,two major chaperones that assist with folding of immature secretory proteins in the ER of rice endosperm cells.High-temperature conditions aggravate ER stress and result in more abnormal grain development in o3 mutants.We also revealed that OsbZIP50 can assist O3 in response to ER stress,especially under high-temperature conditions.We thus demonstrate that O3 plays a central role in rice grain development by participating simultaneously in the regulation of storage protein and starch biosynthesis and the maintenance of ER homeostasis in endosperm cells. | Ruijie Cao Shaolu Zhao Guiai Jiao Yingqing Duan Liuyang Ma Nannan Dong Feifei Lu Mingdong Zhu Gaoneng Shao Shikai Hu Zhonghua Sheng Jian Zhang Shaoqing Tang Xiangjin Wei Peisong Hu | 2022 | Plant Communications2022，3，6： | 0 |
| 17 | Romidepsin(FK228) improves the survival of allogeneic skin grafts through downregulating the production of donor-specific antibody via suppressing the IRE1α-XBP1 pathway显示文摘Antibody-mediated rejection(AMR)is one of the major causes of graft loss after transplantation.Recently,the regulation of B cell differentiation and the prevention of donor-specific antibody(DSA)production have gained increased attention in transplant research.Herein,we established a secondary allogeneic in vivo skin transplant model to study the effects of romidepsin(FK228)on DSA.The survival of grafted skins was monitored daily.The serum levels of DSA and the number of relevant immunocytes in the recipient spleens were evaluated by flow cytometry.Then,we isolated and purified B cells from B6mouse spleens in vitro by magnetic bead sorting.The B cells were cultured with interleukin-4(IL-4)and anti-clusters of differentiation 40(CD40)antibody with or without FK228 treatment.The immunoglobulin G1(IgG1)and IgM levels in the supernatant were evaluated by enzyme-linked immunosorbent assay(ELISA).Quantitative reverse transcription-polymerase chain reaction(RT-q PCR)and western blotting were conducted to determine the corresponding levels of messenger RNA(mRNA)and protein expression in cultured cells and the recipient spleens.The results showed that FK228 significantly improved the survival of allogeneic skin grafts.Moreover,FK228 inhibited DSA production in the serum along with the suppression of histone deacetylase 1(HADC1)and HDAC2 and the upregulation of the acetylation of histones H2A and H3.It also inhibited the differentiation of B cells to plasma cells,decreased the transcription of positive regulatory domain-containing 1(Prdm1)and X-box-binding protein 1(Xbp1),and decreased the expression of phosphorylated inositol-requiring enzyme 1α(p-IRE1α),XBP1,and B lymphocyte-induced maturation protein-1(Blimp-1).In conclusion,FK228 could decrease the production of antibodies by B cells via inhibition of the IRE1α-XBP1 signaling pathway.Thus,FK228 is considered as a promising therapeutic agent for the clinical treatment of AMR. | Yuliang GUO Siyu SONG Xiaoxiao DU Li TIAN Man ZHANG Hongmin ZHOU Zhonghua Klaus CHEN Sheng CHANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，5： | 0 |
| 18 | CACA Guidelines for Holistic Integrative Management of Breast Cancer显示文摘Purpose:Breast cancer is now the most common malignant tumor worldwide.About one-fourth of female cancer patients all over the world sufer from breast cancer.And about one in six female cancer deaths worldwide is caused by breast cancer.In terms of absolute numbers of cases and deaths,China ranks frst in the world.The CACA Guidelines for Holistic Integrative Management of Breast Cancer were edited to help improve the diagnosis and comprehensive treatment in China.Methods:The Grading of Recommendations Assessment,Development and Evaluation(GRADE)was used to classify evidence and consensus.Results:The CACA Guidelines for Holistic Integrative Management of Breast Cancer include the epidemiology of breast cancer,breast cancer screening,breast cancer diagnosis,early breast cancer treatment,advanced breast cancer treatment,follow-up,rehabilitation,and traditional Chinese medicine treatment of breast cancer patients.Conclusion:We to standardize the diagnosis and treatment of breast cancer in China through the formulation of the CACA Guidelines. | Jiong Wu Daiming Fan Zhimin Shao Binghe Xu Guosheng Ren Zefei Jiang Yongsheng Wang Feng Jin Jin Zhang Qingyuan Zhang Fei Ma Jinli Ma Zhonghua Wang Shusen Wang Xiaojia Wang Shu Wang Haibo Wang Tao Wang Xiang Wang Jing Wang Jia Wang Biyun Wang Li Fu Hongyuan Li Yehui Shi Lu Gan Yunjiang Liu Jian Liu Zhenzhen Liu Qiang Liu Qiang Sun Wenwu Cheng Keda Yu Zhongsheng Tong Xinhong Wu Chuangui Song Jianguo Zhang Jian Zhang Junjie Li Bin Li Man Li Huiping Li Wentao Yang Hongjian Yang Benlong Yang Hong Bu Juping Shen Zhenzhou Shen Yiding Chen Ceshi Chen Da Pang Zhimin Fan Ying Zheng Xiaoli Yu Guangyu Liu Xichun Hu Yiqun Ling Jinhai Tang Yongmei Yin Cuizhi Geng Peng Yuan Yajia Gu Cai Chang Xuchen Cao Yuan Sheng Yuanxi Huang Jian Huang Weijun Peng Xiaohua Zeng Yuntao Xie Ning Liao Committee of Breast Cancer Society,Chinese Anti-Cancer Association | 2022 | Holistic Integrative Oncology2022，1，1： | 0 |
| 19 | Thermal Backstripping from Vitrinite Reflectance Profiles in Comparison with an Extensional Modelfor Passive Continental Margin Basins on NorthernContinental Shelf of South China Sea显示文摘Three typical vitrinite reflectance (R0) proriles from Pearl River Mouth, Qiongdongnan and Beibuwan basins on the nortbern condsental shelf or the South China Sea display two or more different sub-linear slope relationships between IgR0 and deptb. According to the reconstruction of sedimentary and burial histories for these wells, a relatively large R0 gradient corresponds to a high depositional rate, while a relatively small R0 gradieut represeuts a low depositional rate. In this study, a modiried thermal backstripping model for paleokeat fIow using vitrinite reflectance data has been used to reconstruct paleotbermal history by the linear-segmeut regression. Tke study results indicate that the gentle parts of a IgR0 profile rerlect abnormai periods or high paleoheat flow, in contrast, the steep parts of a IgR0 profile reflect periods of peleoheat flow decay.In order to have an alternative approach and correlative study, an improved two-layer extensional model for the formation and evolution or rifting basin iu pesive contiueutal margin bas been applied. The model simulates basin subsidence process due to stretcbing and thinuing of continental lithospbere and thermal effects by asthenospbere upwelling, and determines the relationship between subsidence and paleoheat flux through the geological time. The simulation results suggest that these basins have undergone two or three rirting and thermal events, and it is clear that the large R0 gradient segment reflects a response to high paleoheat flow during the early, rapid subsidence stage, while the low R. gradient segmeut is a result or the tbermal decay during the thermal subsidence because of thermal contraction of a cooling Iitbospbere. The results also suggest that the maturity profiles of these basins can be employed to visual understanding tke paleogeothermal characteristics of rifting basins. | Tang Zhonghua(Faculty of Engineering, China University of Geosciences, Wuhan 430074, China)He Sheng(Curtin University of Technology, GPO Box U1987, Perth, W. A. 6001, Australia)Wu Jingfu(Research Center of China Offshore Petroleum Exploration and Developm | 1999 | Journal of Earth Science1999，18，3： | 0 |
| 20 | NRL3 Interacts with OsK4 to Regulate Heading Date in Rice显示文摘NRL3 is essential for the growth and development of rice leaves.In this study,we found that the loss function of NRL3 also delayed heading date under natural long daylight and short daylight conditions.The yeast two-hybrid and the bimolecular fluorescence complementation proved that NRL3interacts with OsK4,a Snf1-related kinase.OsK4 localized to the nucleus and expressed in various rice tissues.The rhythmic expression pattern of Os K4 was similar to NRL3 under long daylight and short daylight conditions.Knock-out mutants of Os K4 exhibited early heading under long daylight conditions,indicating that it acts as a negative regulator of heading date in rice.Interestingly,the OsK4 mutant under the nrl3 mutant background rescued the late heading phenotype of nrl3 under long daylight conditions,suggesting that Os K4 functions downstream of NRL3.Moreover,both NRL3 and Os K4 controlled heading date through regulating the expression of Hd3a and RFT1 genes.These findings shed light on the heading date regulation in rice and provide a sound theoretical base to improve regional adaptability of rice. | CHEN Wei CAI Yicong Shakeel AHMAD WANG Yakun AN Ruihu TANG Shengjia GUO Naihui WEI Xiangjin TANG Shaoqing SHAO Gaoneng JIAO Guiai XIE Lihong HU Shikai SHENG Zhonghua HU Peisong | 2022 | Rice science2022，29，3： | 0 |
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