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| 1 | Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development显示文摘Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(S.typhi) for the candidate vaccine by osmolar stress.Mothods:S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil Nadu.India.Both strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) respectively.The effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5 strains.Attenuation by osmolar stress results in deletion mutation of the.S.typhi slrains was determined by agglutination assays,precipitation method.SDS PAGE analysis and by animal models.Results:The 799 bp amplified ompR gene product from wild type S.typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly,there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl.This illustrates the occurrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore,both the wildtype and mutant S.typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins.In mice,wild type and mutant strains lethal dose(LD_(50)) were determined.The mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response.These results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis.Conclusions:This study demonstrates that the S.typhi strains were allenualed and could be good vaccine candidates in future. | Senthilkumar B Anbarasu K Senbagam D Rajasekarapandian M | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，12： | 2 |
| 2 | Antipyretic,anti-inflammatory and analgesic properties of nilavembu kudineer choornam:a classical preparation used in the treatment of chikungunya fever显示文摘Objective:To investigate the efficacy of ethanolic extract of nilavembu kudineer choornam (EENKC) in inflammation,pain and fever using animal models to support its actions.Methods: Acute toxicity study of EENKC was performed in mice to fix the effective dose.The antipyretic, anti-inflammatory and analgesic activity of EENKC was evaluated in brewer’s yeast induced pyrexia in rats,carrageenan-induced inflammation in rats and acetic-acid induced writhing in mice model.Results:Acute toxicity revealed that EENKC didn’t show death and toxic signs up to 2 000 mg/kg.In brewer’s yeast induced pyrexia and carrageenan-induced inflammation EENKC at the doses of 200 and 400 mg/kg inhibited fever and inflammation significantly(P<0.01 and<0.05) compared to control animals.In mice,the number of writhing induced by acetic-acid was significantly(P<0.01) reduced after treatment with both the dose of EENKC than control animals. EENKC 200 mg/kg inhibits inflammation higher level in carrageenan-induced paw edema,but there is no significant difference when compared to indomethacin 10 mg/kg.Conclusions:The present findings revealed that EENKC possesses antipyretic,anti-inflammatory and analgesic activity which supports nilavembu kudineer choornam efficacy in chikungunya fever. | Anbarasu K Manisenthil Kumar KT Ramachandran S | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，10： | 2 |
| 3 | Effects of ascorbic acid on the immune response of the catfish,Mystus gulio(Hamilton),to different bacterins of Ameromonas hydrophila显示文摘 | Anbarasu K Chandran M R | 2001 | Fish Shellfish Immunol2001，11，： | 1 |
| 4 | Effects of ascorbic acid on the immune response of the catfish, Mystus gulio (Hamilton), to different bacterins of Ameromonas hydrophila 显示文摘 | ANBARASU K CHANDRAN M R | 2001 | Fish & Shellfish Immunology2001，11，： | 1 |
| 5 | Mechanism of activation of the Lanta Khola landslide in Sikkim Himalayas显示文摘 | K Anbarasu A Sengupta S Guputa | 2010 | Landslides2010，7，2： | 1 |
| 6 | Effects of ascorbic acid on the immune response of the catfish,Mystus gulio (Hamilton),to different bacterins of Ameromonas hydrophila显示文摘 | Anbarasu K Chandran M R | 2001 | Fish Shellfish Immunol2001，11，： | 1 |
| 7 | Effect of growth factors and ro-inflammatory cytokines by the collagen biocomposite dressing material containing Macrotyloma uniflorum plant extract-In vivo wound healing显示文摘 | Muthukumar T Anbarasu K Prakash D | 2014 | Colloids Surf B Biointerfaces2014，121，： | 1 |
| 8 | Effect of growth factors and pro-inflammatory cytokines by thecollagen biocomposite dressing material containing Macrotylomauniflorum plant extract-in vivo wound healing显示文摘 | Muthukumar T Anbarasu K Prakash D | 2014 | Colloids and Surfaces B: Biointerfaces2014，121，： | 1 |
| 9 | Effect of growth factors and pro-inflammatory cytokines by the collagen biocom- posite dressing material containing Macrotyloma uniflorum plant extract-In vivo wound healing 显示文摘 | Muthukumar T Anbarasu K Prakash D | 2014 | Colloids Surf B Biointerfac es2014，1，121： | 1 |
| 10 | The E3ubiquitin ligase Itch regulates tumor suppressor protein RASSF5/NORE1stability in an acetylation-dependent manner显示文摘 | Suryaraja R Anitha M Anbarasu K | 2013 | Cell Death Dis2013，4，： | 1 |
| 11 | Rainfall thresholds for the initiation of landslide at Lanta Khola in north Sikkim,India显示文摘 | SENGUPTA A GUPTA S ANBARASU K | 2010 | Natural Hazards2010，52，1： | 1 |
| 12 | Bio-diesel production by alkali catalyzed transesterification of dairy waste scum显示文摘 | SIVAKUMAR P ANBARASU K RENGANATHAN S | | 0，，01： | 1 |
| 13 | Protective effect of Pisonia aculeata on thioacetamide induced hepatotoxicity in rats显示文摘Objective:To evaluate the protective effect of Pisonia aculeata(P.aculeata) on thioacetamide induced hepatotoxicity in rats.Methods:Male Wistar rats were administered 250 or 500 mg/kg p.o.of P.aculeata extract for 21 days and simultaneously administered thioacetamide(TAA) 50 mg/kg bw s.c.1 h alter the respective assigned treatments every 72 h.At the end of all experimental methods,all the animals were sacrificed by cervical decapitation.Blood samples were collected.Serum was separated and analyzed for various biochemical parameters.Results:TAA induced a significant rise in aspartate amino transferase(AST),alanine amino transferase(ALT),alkaline phosphatase(ALP),total bilirubin,gamma glutamate transpeptidase(GGTP),lipid peroxidase(LPO)with a reduction of total protein,superoxide dismutase(SOD),catalase,glutathione peroxidase(GPx) and glutathione S-transferase(GST).Treatment of rats with different does of plant extract(250 and 500 mg/kg) significantly(P<0.001) altered serum marker enzymes and antioxidant levels to near normal against TAA treated rats.The activity of the extract at a dose of 300 tug/kg was comparable to the standard drug,silymarin(50 mg/kg.p.o.).Conclusions:It can be concluded that P.aculeata extract possesses a remarkable hepaloprolective and antioxidant activity against TAA induced hepatotoxicitv.Wore research is required lo derive an optimal therapeutic dose. | Anbarasu C Rajkapoor B Bhat KS John Giridharan A Arul Amuthan Satish K | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，7： | 0 |
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